{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T04:59:21Z","timestamp":1785560361412,"version":"3.56.0"},"reference-count":42,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Autonomous University of Mexico"},{"id":[{"id":"https:\/\/ror.org\/01tmp8f25","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Conde de Valenciana Foundation IAP"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Clin. Med.","JCM"],"abstract":"<jats:p>Background\/Objectives: Allergic conjunctivitis (AC) is a frequent inflammatory ocular surface disease that significantly affects quality of life, particularly in children. Current treatments mainly provide temporary symptom relief and often require prolonged use. Bacterial suspensions have emerged as potential immunomodulatory treatments for other allergies but have yet to be completely explored for ocular allergies. Our study\u2019s objective was to describe the clinical, ophthalmological, and quality-of-life changes in patients with AC treated with a bacterial suspension (BS) as complementary therapy. Methods: A before-and-after open, non-controlled clinical study was conducted in five children aged 7 to 9 years with a diagnosis of moderate-to-severe persistent allergic conjunctivitis and negative skin prick test results. Clinical ocular signs and symptoms, quality of life, and changes in CD19+IL-10+ cells were assessed. Results: After 90 days of BS treatment, reductions in allergic symptoms, including itching, light sensitivity, and burning, were observed, along with a marked reduction in ocular inflammation. An evaluation of quality-of-life revealed improvements across all domains and an increase in CD19+IL-10+ cells. Conclusions: BS therapy demonstrated favorable clinical and immune-modulatory effects in children with AC, supporting its potential as a promising complementary therapeutic option.<\/jats:p>","DOI":"10.3390\/jcm15155962","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:55:11Z","timestamp":1785419711000},"page":"5962","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Clinical Effect of Bacterial Suspension in Patients with Moderate-to-Severe Allergic Conjunctivitis"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0399-651X","authenticated-orcid":false,"given":"Israel","family":"Casanova-M\u00e9ndez","sequence":"first","affiliation":[{"name":"Department of Non-Ophthalmological Emergencies, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"},{"name":"Department of Immunology and Research Unit, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillermo A.","family":"Quintana-Mexiac","sequence":"additional","affiliation":[{"name":"Department of Immunology and Research Unit, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 L.","family":"Alcal\u00e1-Gallegos","sequence":"additional","affiliation":[{"name":"Department of Non-Ophthalmological Emergencies, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"},{"name":"Department of Immunology and Research Unit, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7405-4875","authenticated-orcid":false,"given":"Henry","family":"Velazquez-Soto","sequence":"additional","affiliation":[{"name":"Department of Immunology and Research Unit, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5960-5715","authenticated-orcid":false,"given":"Lorenzo","family":"Islas-V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Department of Immunology and Research Unit, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"},{"name":"Pharmacovigilance Unit, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Pacheco-Quito","sequence":"additional","affiliation":[{"name":"Department of Cornea and Refractive Surgery, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concepci\u00f3n","family":"Santacruz-Vald\u00e9s","sequence":"additional","affiliation":[{"name":"Department of Cornea and Refractive Surgery, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3982-9097","authenticated-orcid":false,"given":"Mar\u00eda C.","family":"Jim\u00e9nez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Immunology and Research Unit, Instituto de Oftalmolog\u00eda \u201cConde de Valenciana\u201d, Ciudad de Mexico 06800, Mexico"},{"name":"Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico, Ciudad de Mexico 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Tariq, F. 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