{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T11:28:45Z","timestamp":1787225325789,"version":"build-2736575974"},"reference-count":26,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009594","name":"University of P\u00e9cs","doi-asserted-by":"publisher","award":["PTE-LJDKO-2025-149"],"award-info":[{"award-number":["PTE-LJDKO-2025-149"]}],"id":[{"id":"10.13039\/501100009594","id-type":"DOI","asserted-by":"publisher"}]},{"award":["PTE-LJDKO-2025-149"],"award-info":[{"award-number":["PTE-LJDKO-2025-149"]}],"id":[{"id":"https:\/\/ror.org\/037b5pv06","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Clin. Med.","JCM"],"abstract":"<jats:p>Objectives: The aim of this study was to evaluate the reliability, accuracy, and morbidity of dynamic sentinel lymph node biopsy (DSNB) in patients with intermediate- and high-risk penile cancer who had clinically negative lymph nodes, with a focus on long-term follow-up outcomes. Methods: Between January 2005 and January 2024, 287 patients with histologically confirmed penile cancer underwent radioisotope-guided DSNB at the Department of Urology, University of P\u00e9cs. Patients lost to follow-up within 24 months were excluded. Of the remaining patients, 211 underwent primary DSNB and 37 secondary DSNB. Thirty-nine patients in the primary DSNB group with Tis, T1a, or G1 tumours were additionally excluded, leaving 209 patients for analysis. Variables included tumour stage and grade, sentinel lymph node (SLN) yield and histology, complications, inguinal progression, false-negative rate, and radical lymphadenectomy findings. Results: A total of 573 SLNs were removed from 209 patients, with a median of 2.7 nodes per patient. SLN metastases were identified in 31 patients, involving 37 groins. Among 178 patients with negative SLNs, three developed inguinal metastases. Sensitivity was 91% per patient and 92.5% per groin, with false-negative rates of 8% and 7.5%, respectively. All false-negative cases occurred within two years, with no later inguinal recurrences. DSNB-related complications occurred in 24 patients (11.4%); most were mild or moderate and managed on an outpatient basis. Two patients required surgery and one required hospital readmission. Conclusions: In a high-volume centre, DSNB is a safe and reliable staging procedure with low morbidity in clinically node-negative intermediate- and high-risk penile cancer. Further studies should assess radiotracer injection sites and compare one-day versus two-day protocols.<\/jats:p>","DOI":"10.3390\/jcm15166449","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T10:55:18Z","timestamp":1787223318000},"page":"6449","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long-Term Follow-Up of the Reliability, Accuracy, and Morbidity of Dynamic Sentinel Lymph Node Biopsy in Patients with Intermediate- and High-Risk Penile Cancer and Clinically Negative Lymph Node Status"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0381-5174","authenticated-orcid":false,"given":"\u00c1kos","family":"Pytel","sequence":"first","affiliation":[{"name":"Department of Urology, Medical School, University of P\u00e9cs, Munkacsy Mihaly u. 2., H-7621 Pecs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9903-5006","authenticated-orcid":false,"given":"Bence","family":"Pytel","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical School, University of P\u00e9cs, Szigeti u. 12., H-7624 Pecs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u00e1vid","family":"Semj\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Pathology, Medical School, University of P\u00e9cs, Szigeti u. 12., H-7624 Pecs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2898-1571","authenticated-orcid":false,"given":"Zsombor","family":"Ritter","sequence":"additional","affiliation":[{"name":"Department of Medical Imaging, Division of Nuclear Medicine, Medical School, University of P\u00e9cs, Ifjusag u. 13., H-7624 Pecs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"ref_1","first-page":"e117","article-title":"Incidence of penile cancer worldwide: Systematic review and meta-analysis","volume":"41","author":"Cardona","year":"2018","journal-title":"Rev. Panam. Salud P\u00fablica"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.eururo.2026.03.022","article-title":"European Association of Urology and American Society of Clinical Oncology Guidelines on Penile Cancer: A Summary of the 2026 Guidelines Update","volume":"90","author":"Parnham","year":"2026","journal-title":"Eur. Urol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S66","DOI":"10.1016\/j.urology.2010.04.008","article-title":"Prognostic factors in penile cancer","volume":"76","author":"Ficarra","year":"2010","journal-title":"Urology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1200\/OP.23.00585","article-title":"Penile Cancer: EAU-ASCO Collaborative Guidelines Update Q and A","volume":"20","author":"Brouwer","year":"2024","journal-title":"JCO Oncol. Pract."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1097\/01.ju.0000154565.37397.4d","article-title":"Patients with penile carcinoma benefit from immediate resection of clinically occult lymph node metastases","volume":"173","author":"Kroon","year":"2005","journal-title":"J. Urol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1002\/1097-0142(197702)39:2<456::AID-CNCR2820390214>3.0.CO;2-I","article-title":"An approach for the treatment of penile carcinoma","volume":"39","author":"Cabanas","year":"1977","journal-title":"Cancer"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1007\/s00345-019-02866-9","article-title":"Making surgery safer by centralization of care: Impact of case load in penile cancer","volume":"38","author":"Vanthoor","year":"2020","journal-title":"World J. Urol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"879","DOI":"10.21037\/tau.2018.08.01","article-title":"Detection of lymph node metastases in penile cancer","volume":"7","author":"Bloom","year":"2018","journal-title":"Transl. Androl. Urol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1364","DOI":"10.1016\/S0022-5347(01)66863-0","article-title":"Carcinoma of penis: Improved survival by early regional lymphadenectomy based on histological grade and depth of invasion of primary lesion","volume":"154","author":"McDougal","year":"1995","journal-title":"J. Urol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1638","DOI":"10.1016\/S0022-5347(05)65169-5","article-title":"Contemporary morbidity from lymphadenectomy for penile squamous cell carcinoma: The MD Anderson Cancer Center Experience","volume":"167","author":"Slaton","year":"2002","journal-title":"J. Urol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"486","DOI":"10.1016\/j.eururo.2013.02.037","article-title":"Early wound complications after inguinal lymphadenectomy in penile cancer: A historical cohort study and risk-factor analysis","volume":"64","author":"Stuiver","year":"2013","journal-title":"Eur. Urol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/S0022-5347(05)67982-7","article-title":"Detection of occult metastasis in squamous cell carcinoma of the penis using a dynamic sentinel node procedure","volume":"163","author":"Horenblas","year":"2000","journal-title":"J. Urol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1007\/s002400000125","article-title":"First experience with gamma probe guided sentinel lymph node surgery in penile cancer","volume":"28","author":"Wawroschek","year":"2000","journal-title":"Urol. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e393","DOI":"10.1016\/j.juro.2011.02.986","article-title":"975 Advantages of Dynamic Sentinel Lymphnode Biopsy Technique in the Management of Penile Cancer","volume":"185","author":"Pytel","year":"2011","journal-title":"J. Urol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Cuesta, M.A., and Bonjer, H.J. (2014). The Clavien\u2013Dindo Classification of Surgical Complications. Treatment of Postoperative Complications After Digestive Surgery, Springer.","DOI":"10.1007\/978-1-4471-4354-3"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.euf.2022.11.018","article-title":"Diagnostic accuracy of dynamic sentinel lymph node biopsy for penile cancer: A systematic review and meta-analysis","volume":"9","author":"Fallara","year":"2023","journal-title":"Eur. Urol. Focus"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1007\/s11255-016-1405-x","article-title":"Radiocolloid-based dynamic sentinel lymph node biopsy in penile cancer with clinically negative inguinal lymph node: An updated systematic review and meta-analysis","volume":"48","author":"Zou","year":"2016","journal-title":"Int. Urol. Nephrol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3325","DOI":"10.1200\/JCO.2008.20.6870","article-title":"Two-center evaluation of dynamic sentinel node biopsy for squamous cell carcinoma of the penis","volume":"27","author":"Leijte","year":"2009","journal-title":"J. Clin. Oncol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1016\/j.eururo.2012.10.035","article-title":"Dynamic sentinel lymph node biopsy in patients with invasive squamous cell carcinoma of the penis: A prospective study of the long-term outcome of 500 inguinal basins assessed at a single institution","volume":"63","author":"Lam","year":"2013","journal-title":"Eur. Urol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Nemitz, L., Vincke, A., Michalik, B., Engels, S., Meyer, L.-M., Henke, R.-P., Wawroschek, F., and Winter, A. (2022). Radioisotope-guided sentinel lymph node biopsy in penile cancer: A long-term follow-up study. Front. Oncol., 12.","DOI":"10.3389\/fonc.2022.850905"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1111\/bju.13389","article-title":"Dynamic sentinel lymph node biopsy for penile cancer: A comparison between 1-and 2-day protocols","volume":"117","author":"Dimopoulos","year":"2016","journal-title":"BJU Int."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Murta, C.B., Furuya, T.K., Carrasco, A.G.M., Uno, M., Sichero, L., Villa, L.L., Faraj, S.F., Coelho, R.F., Guglielmetti, G.B., and Cordeiro, M.D. (2022). miRNA and mRNA Expression Profiles Associated with Lymph Node Metastasis and Prognosis in Penile Carcinoma. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23137103"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.1007\/s00259-023-06290-5","article-title":"(99m)Tc-Tilmanocept performance for sentinel node mapping in breast cancer, melanoma, and head and neck cancer: A systematic review and meta-analysis from a European expert panel","volume":"50","author":"Rovera","year":"2023","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.eururo.2020.09.007","article-title":"Hybrid Indocyanine Green-(99m)Tc-nanocolloid for Single-photon Emission Computed Tomography and Combined Radio- and Fluorescence-guided Sentinel Node Biopsy in Penile Cancer: Results of 740 Inguinal Basins Assessed at a Single Institution","volume":"78","author":"Mazzone","year":"2020","journal-title":"Eur. Urol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1016\/j.euo.2022.09.004","article-title":"Sentinel Node Identification with Hybrid Tracer-guided and Conventional Dynamic Sentinel Node Biopsy in Penile Cancer: A Prospective Study in 130 Patients from the Two National Referral Centres in Sweden","volume":"5","author":"Torbrand","year":"2022","journal-title":"Eur. Urol. Oncol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Michalik, B., Engels, S., Otterbach, M.C., Maurer, M.H., Wawroschek, F., and Winter, A. (2025). Bimodal inguinal sentinel lymph node imaging using a radiation-free fluorescent magnetic hybrid tracer in penile cancer patients. Front. 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