{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T20:58:48Z","timestamp":1788901128206,"version":"build-2803163510"},"reference-count":37,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2018,12,20]],"date-time":"2018-12-20T00:00:00Z","timestamp":1545264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>This report presents the case of a woman aged 40 who has experienced premature menopause from the age of 35. Having rejected oocyte donation, she opted for intraovarian injection of autologous platelet-rich plasma with the aim to rejuvenate the ovarian tissue and enable the employment of her own gametes through in-vitro fertilization. Six weeks following the autologous platelet-rich plasma treatment, a significant reduction in the patient\u2019s follicle-stimulating hormone (FSH) levels were noted. A natural in-vitro fertilization cycle led to a biochemical pregnancy, resulting in a spontaneous abortion at the 5th week of pregnancy. This is the first report of a successful autologous platelet-rich plasma application leading to pregnancy in menopause. This report uniquely contributes to the medical knowledge and challenges current practice in the context of infertility. The efficiency and safety of this treatment with regard to the reproductive system merits further investigation.<\/jats:p>","DOI":"10.3390\/jcm8010001","type":"journal-article","created":{"date-parts":[[2018,12,20]],"date-time":"2018-12-20T12:54:36Z","timestamp":1545310476000},"page":"1","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Autologous Platelet-Rich Plasma Treatment Enables Pregnancy for a Woman in Premature Menopause"],"prefix":"10.3390","volume":"8","author":[{"given":"Konstantinos","family":"Sfakianoudis","sequence":"first","affiliation":[{"name":"Centre for Human Reproduction, Genesis Athens Clinic, 14-16, Papanikoli, 15232 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1000-9100","authenticated-orcid":false,"given":"Mara","family":"Simopoulou","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical School, National and Kapodistrian University of Athens, 75, Mikras Asias, 11527 Athens, Greece"},{"name":"Assisted Conception Unit, 2nd Department of Obstetrics and Gynecology, Aretaieion Hospital, Medical School, National and Kapodistrian University of Athens, 76, Vasilisis Sofias Avenue, 11528 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaos","family":"Nitsos","sequence":"additional","affiliation":[{"name":"Centre for Human Reproduction, Genesis Athens Clinic, 14-16, Papanikoli, 15232 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7636-5848","authenticated-orcid":false,"given":"Anna","family":"Rapani","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical School, National and Kapodistrian University of Athens, 75, Mikras Asias, 11527 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athanasios","family":"Pappas","sequence":"additional","affiliation":[{"name":"Centre for Human Reproduction, Genesis Athens Clinic, 14-16, Papanikoli, 15232 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agni","family":"Pantou","sequence":"additional","affiliation":[{"name":"Centre for Human Reproduction, Genesis Athens Clinic, 14-16, Papanikoli, 15232 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Chronopoulou","sequence":"additional","affiliation":[{"name":"Centre for Human Reproduction, Genesis Athens Clinic, 14-16, Papanikoli, 15232 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efthymios","family":"Deligeoroglou","sequence":"additional","affiliation":[{"name":"Assisted Conception Unit, 2nd Department of Obstetrics and Gynecology, Aretaieion Hospital, Medical School, National and Kapodistrian University of Athens, 76, Vasilisis Sofias Avenue, 11528 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Koutsilieris","sequence":"additional","affiliation":[{"name":"Department of Physiology, Medical School, National and Kapodistrian University of Athens, 75, Mikras Asias, 11527 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos","family":"Pantos","sequence":"additional","affiliation":[{"name":"Centre for Human Reproduction, Genesis Athens Clinic, 14-16, Papanikoli, 15232 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1097\/01.AOG.0000270153.59102.40","article-title":"Symptoms associated with menopausal transition and reproductive hormones in midlife women","volume":"110","author":"Freeman","year":"2007","journal-title":"Obstet. Gynecol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1093\/humrep\/deg005","article-title":"Premature menopause in a multi-ethnic population study of the menopause transition","volume":"18","author":"Luborsky","year":"2003","journal-title":"Hum. Reprod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.maturitas.2009.08.003","article-title":"Premature menopause or early menopause: Long-term health consequences","volume":"65","author":"Shuster","year":"2010","journal-title":"Maturitas"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.ecl.2015.05.004","article-title":"Premature menopause","volume":"44","author":"Torrealday","year":"2015","journal-title":"Endocrinol. Metab. Clin."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1097\/AOG.0b013e3181a66843","article-title":"Premature ovarian failure","volume":"113","author":"Rebar","year":"2009","journal-title":"Obstet. Gynecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/j.rbmo.2017.04.007","article-title":"Platelet-rich plasma promotes the development of isolated human primordial and primary follicles to the preantral stage","volume":"35","author":"Hosseini","year":"2017","journal-title":"Reprod. Biomed. Online"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1159\/000440617","article-title":"Protective effect of platelet rich plasma on experimental ischemia\/reperfusion injury in rat ovary","volume":"81","author":"Bakacak","year":"2016","journal-title":"Gynecol. Obstet. Investig."},{"key":"ref_8","first-page":"1286","article-title":"Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization","volume":"8","author":"Chang","year":"2015","journal-title":"Int. J. Clin. Exp. Med."},{"key":"ref_9","first-page":"179","article-title":"Use of platelet rich plasma in human infertility","volume":"31","author":"Colombo","year":"2017","journal-title":"J. Biol. Regul. Homeost. Agents"},{"key":"ref_10","first-page":"42","article-title":"Platelet-rich plasma as an adjuvant in the endometrial preparation of patients with refractory endometrium","volume":"22","author":"Molina","year":"2018","journal-title":"JBRA Assist. Reprod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"54","DOI":"10.5935\/1518-0557.20170013","article-title":"Treatment of thin endometrium with autologous platelet-rich plasma: A pilot study","volume":"21","author":"Zadehmodarres","year":"2017","journal-title":"JBRA Assist. Reprod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1159\/000046610","article-title":"The role of vascular endothelial growth factor in angiogenesis","volume":"106","author":"Ferrara","year":"2001","journal-title":"Acta Haematol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"173","DOI":"10.3109\/09537104.2012.684730","article-title":"The role of platelet-rich plasma in tissue regeneration","volume":"24","author":"Alsousou","year":"2013","journal-title":"Platelets"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1002\/jor.20278","article-title":"Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons","volume":"25","author":"Schnabel","year":"2007","journal-title":"J. Orthop. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2714","DOI":"10.1089\/ten.tea.2015.0159","article-title":"The Effects of Platelet-Rich Plasma on Cell Proliferation and Adipogenic Potential of Adipose-Derived Stem Cells","volume":"21","author":"Liao","year":"2015","journal-title":"Tissue Eng Part A"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.mvr.2013.04.008","article-title":"Platelet rich plasma extract promotes angiogenesis through the angiopoietin1-Tie2 pathway","volume":"89","author":"Mammoto","year":"2013","journal-title":"Microvasc. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1007\/s10815-018-1159-8","article-title":"Platelet-rich plasma therapy and reproductive medicine","volume":"35","author":"Frantz","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1159\/000356329","article-title":"Bioactive Factors in Platelet-Rich Plasma Obtained by Apheresis","volume":"40","author":"Freymannx","year":"2013","journal-title":"Transfus. Med. Hemother."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1038\/nm.2669","article-title":"Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women","volume":"18","author":"White","year":"2012","journal-title":"Nat. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.1016\/j.fertnstert.2011.03.045","article-title":"Influence of follicular fluid GDF9 and BMP15 on embryo quality","volume":"95","author":"Gode","year":"2011","journal-title":"Fertil. Steril."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/mrd.21265","article-title":"Integral Role of GDF-9 and BMP-15 in Ovarian Function","volume":"78","author":"Otsuka","year":"2011","journal-title":"Mol. Reprod. Dev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1080\/09513590.2018.1445219","article-title":"First data on in vitro fertilization and blastocyst formation after intraovarian injection of calcium gluconate-activated autologous platelet rich plasma","volume":"34","author":"Sills","year":"2018","journal-title":"Gynecol. Endocrinol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2459","DOI":"10.1007\/s00167-013-2743-1","article-title":"Platelet-rich plasma: Why intra-articular? A systematic review of preclinical studies and clinical evidence on PRP for joint degeneration","volume":"23","author":"Filardo","year":"2015","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/jocd.12167","article-title":"Systematic review of the use of platelet-rich plasma in aesthetic dermatology","volume":"14","author":"Leo","year":"2015","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2546","DOI":"10.1056\/NEJMc1402340","article-title":"Platelet-rich plasma injections in acute muscle injury","volume":"370","author":"Reurink","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s12958-016-0195-4","article-title":"Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro","volume":"14","author":"Marini","year":"2016","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sfakianoudis, K., Simopoulou, M., Nitsos, N., Rapani, A., Pantou, A., Vaxevanoglou, T., Kokkali, G., Koutsilieris, M., and Pantos, K. (2018). A Case Series on Platelet-Rich Plasma Revolutionary Management of Poor Responder Patients. Gynecol. Obstet. Investig., 1\u20138.","DOI":"10.1159\/000491697"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Veeck, L.L. (1999). An Atlas of Human Gametes and Conceptuses: An Illustrated Reference for Assisted Reproductive Technology, CRC Press.","DOI":"10.1201\/b14639"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1757-2215-6-33","article-title":"Live birth in a woman without ovaries after autograft of frozen-thawed ovarian tissue combined with growth factors","volume":"6","author":"Callejo","year":"2013","journal-title":"J. Ovarian Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1002\/ajh.21732","article-title":"Normal ranges of angiogenesis regulatory proteins in human platelets","volume":"85","author":"Peterson","year":"2010","journal-title":"Am. J. Hematol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/1477-7827-3-17","article-title":"Oogenesis in cultures derived from adult human ovaries","volume":"3","author":"Bukovsky","year":"2005","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1111\/j.1432-0436.2008.00268.x","article-title":"Putative stem cells with an embryonic character isolated from the ovarian surface epithelium of women with no naturally present follicles and oocytes","volume":"76","author":"Zech","year":"2008","journal-title":"Differentiation"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1007\/s10815-018-1141-5","article-title":"Multi-center clinical evaluation of the Access AMH assay to determine AMH levels in reproductive age women during normal menstrual cycles","volume":"35","author":"Gracia","year":"2018","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0378-5122(95)00969-8","article-title":"The endocrinology of the menopause","volume":"23","author":"Burger","year":"1996","journal-title":"Maturitas"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1016\/j.bpobgyn.2014.09.003","article-title":"Transplantation of ovarian tissue","volume":"28","author":"Donnez","year":"2014","journal-title":"Best Prac. Res. Clin. Obstet. Gynaecol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"769","DOI":"10.3109\/09513590903004126","article-title":"Hormone replacement therapy and successful pregnancy in a patient with premature ovarian failure","volume":"25","author":"Popovac","year":"2009","journal-title":"Gynecol. Endocrinol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1093\/humupd\/5.5.483","article-title":"Premature ovarian failure: A systematic review on therapeutic interventions to restore ovarian function and achieve pregnancy","volume":"5","author":"Schoemaker","year":"1999","journal-title":"Hum. Reprod. 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