{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T11:23:58Z","timestamp":1772882638936,"version":"3.50.1"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,2,23]],"date-time":"2019-02-23T00:00:00Z","timestamp":1550880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>The autoclaving process is one of the sterilization procedures of implantable devices. Therefore, it is important to assess the impact of hot steam at high pressure on the morphology, structure, and properties of implants modified by nanocomposite coatings. In our works, we focused on studies on amorphous titania nanotubes produced by titanium alloy (Ti6Al4V) electrochemical oxidation in the potential range 5\u201360 V. Half of the samples were drying in argon stream at room temperature, and the second ones were drying additionally with the use of immersion in acetone and drying at 396 K. Samples were subjected to autoclaving and after sterilization they were structurally and morphologically characterized using Raman spectroscopy, diffuse reflectance infrared Fourier transform spectroscopy (DRIFT) and scanning electron microscopy (SEM). They were characterized in terms of wettability, mechanical properties, and biocompatibility. Obtained results proved that the autoclaving of amorphous titania nanotube coatings produced at lower potentials (5\u201315 V) does not affect their morphology and structure regardless of the drying method before autoclaving. Nanotubular coatings produced using higher potentials (20\u201360 V) require removal of adsorbed water particles from their surface. Otherwise, autoclaving leads to the destruction of the architecture of nanotubular coatings, which is associated with the changing of their mechanical and biointegration properties.<\/jats:p>","DOI":"10.3390\/jcm8020272","type":"journal-article","created":{"date-parts":[[2019,2,25]],"date-time":"2019-02-25T03:06:52Z","timestamp":1551064012000},"page":"272","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The Morphology, Structure, Mechanical Properties and Biocompatibility of Nanotubular Titania Coatings before and after Autoclaving Process"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2526-0779","authenticated-orcid":false,"given":"Aleksandra","family":"Radtke","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Nicolaus Copernicus University in Toru\u0144, Gagarina 7, 87-100 Toru\u0144, Poland"},{"name":"Nano-implant Ltd., Gagarina 5\/102, 87-100 Toru\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michalina","family":"Ehlert","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Nicolaus Copernicus University in Toru\u0144, Gagarina 7, 87-100 Toru\u0144, Poland"},{"name":"Nano-implant Ltd., Gagarina 5\/102, 87-100 Toru\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8950-5643","authenticated-orcid":false,"given":"Tomasz","family":"J\u0119drzejewski","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Environmental Protection, Nicolaus Copernicus University in Toru\u0144, Lwowska 1, 87-100 Toru\u0144, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0185-6460","authenticated-orcid":false,"given":"Micha\u0142","family":"Bartma\u0144ski","sequence":"additional","affiliation":[{"name":"Faculty of Mechanical Engineering, Gda\u0144sk University of Technology, Gabriela Narutowicza 11\/12, 80-233 Gda\u0144sk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,23]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Osteogenic and bactericidal surfaces from hydrothermal titania nanowires on titanium substrates","volume":"18","author":"Tsimbouri","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/2041731418789838","article-title":"Antibacterial surface modification of titanium implants in orthopaedics","volume":"9","author":"Orapiriyakul","year":"2018","journal-title":"J. Tissue Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"591","DOI":"10.5312\/wjo.v5.i5.591","article-title":"Survival outcomes of cemented compared to uncemented stems in primary total hip replacement","volume":"5","author":"Wyatt","year":"2014","journal-title":"World J. Orthop."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/srep19047","article-title":"Ti nanorod arrays with a medium density significantly promote osteogenesis and osteointegration","volume":"6","author":"Ning","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.apsusc.2014.04.207","article-title":"Hydroxyapatite electrodeposition on anodized titanium nanotubes for orthopedic applications","volume":"311","author":"Parcharoen","year":"2014","journal-title":"Appl. Surf. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.msec.2017.03.148","article-title":"TiO2 nanorod arrays modified Ti substrates promote the adhesion, proliferation and osteogenic differentiation of human periodontal ligament stem cells","volume":"76","author":"Li","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3467","DOI":"10.1016\/j.biomaterials.2013.01.071","article-title":"Osteogenic activity and antibacterial effects on titanium surfaces modified with Zn-incorporated nanotube arrays","volume":"34","author":"Huo","year":"2013","journal-title":"Biomaterials"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2813","DOI":"10.1016\/j.biomaterials.2012.01.018","article-title":"Balancing osteoblast functions and bacterial adhesion on functionalized titanium surfaces","volume":"33","author":"Neoh","year":"2012","journal-title":"Biomaterials"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5257","DOI":"10.1002\/adma.201301762","article-title":"Cell-material interactions revealed via material techniques of surface patterning","volume":"25","author":"Yao","year":"2013","journal-title":"Adv. Mater."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1034\/j.1600-0501.1997.080601.x","article-title":"Osseointegration enhanced by chemical etching of the titanium surface. A torque removal study in the rabbit","volume":"8","author":"Klokkevold","year":"1997","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1111\/j.1600-0501.2004.01054.x","article-title":"A systematic review of the influence of different titanium surfaces on proliferation, differentiation and protein synthesis of osteoblast-like MG63 cells","volume":"15","author":"Kohal","year":"2004","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Radtke, A., Topolski, A., J\u0119drzejewski, T., Kozak, W., Sadowska, B., Wi\u0119ckowska-Szakiel, M., and Piszczek, P. (2017). Bioactivity Studies on Titania Coatings and the Estimation of Their Usefulness in the Modification of Implant Surfaces. Nanomaterials, 7.","DOI":"10.3390\/nano7040090"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1016\/j.electacta.2018.03.180","article-title":"Electrochemical surface engineering of titanium-based alloys for biomedical application","volume":"271","author":"Gao","year":"2018","journal-title":"Electrochim. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1039\/C7TB03251J","article-title":"Micro- and Nano-structured 3D Printed Titanium Implants with Hydroxyapatite Coating for Improved Osseointegration","volume":"6","author":"Qin","year":"2018","journal-title":"J. Mater. Chem. B"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/j.dental.2006.06.025","article-title":"Surface treatments of titanium dental implants for rapid osseointegration","volume":"23","author":"Soueidan","year":"2007","journal-title":"Dent. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3331","DOI":"10.1557\/JMR.2001.0457","article-title":"Titanium oxide nanotube arrays prepared by anodic oxidation","volume":"16","author":"Gong","year":"2001","journal-title":"J. Mater. Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Radtke, A., Bal, M., and J\u0119drzejewski, T. (2018). Novel Titania Nanocoatings Produced by Anodic Oxidation with the Use of Cyclically Changing Potential: Their Photocatalytic Activity and Biocompatibility. Nanomaterials, 8.","DOI":"10.3390\/nano8090712"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1021\/cm802384y","article-title":"TiO2 Nanotubes with Tunable Morphology, Diameter and Length: Synthesis and Photo-Elecrtical\/Catalytic Performance","volume":"21","author":"Wang","year":"2009","journal-title":"Chem. Mater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.jphotochem.2017.04.015","article-title":"TiO2 nanotube arrays formation in fluoride\/ethylene glycol electrolyte containing LiOH or KOH as photoanode for dye-sensitized solar cell","volume":"343","author":"Nyein","year":"2017","journal-title":"J. Photochem. Photobiol. A Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.matdes.2017.04.097","article-title":"Initial growth study of TiO2 nanotube arrays anodised in KOH\/fluoride\/ethylene glycol electrolyte","volume":"128","author":"Tai","year":"2017","journal-title":"Mater. Des."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1016\/j.surfcoat.2017.05.016","article-title":"Preparation and characterization of TiO2 nanotube arrays on Ti6Al4V surface for enhancement of cell treatment","volume":"321","author":"Mansoorianfar","year":"2017","journal-title":"Surf. Coat. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3215","DOI":"10.1016\/j.actbio.2009.05.008","article-title":"Improved bone forming functionality on diameter-controlled TiO2 nanotube surface","volume":"5","author":"Brammer","year":"2009","journal-title":"Acta Biomater."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1002\/jbm.a.32088","article-title":"TiO2 nanotubes on Ti: Influence of nanoscale morphology on bone cell\u2013materials interaction","volume":"90","author":"Das","year":"2009","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1002\/smll.200801476","article-title":"TiO2 nanotube surfaces: 15 nm\u2014An optimal length scale of surface topography for cell adhesion and differentiation","volume":"5","author":"Park","year":"2009","journal-title":"Small"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Radtke, A., Topolski, A., J\u0119drzejewski, T., Sadowska, B., Wi\u0119ckowska-Szakiel, M., Szubka, M., Talik, E., Nielsen, L.P., and Piszczek, P. (2017). The bioactivity and photocatalytic properties of titania nanotube coatings produced with the use of the low-potential anodization of Ti6Al4V alloy surface. Nanomaterials, 7.","DOI":"10.3390\/nano7080197"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1016\/j.biomaterials.2009.11.103","article-title":"The role of sterilization in the cytocompatibility of titania nanotubes","volume":"31","author":"Zhao","year":"2010","journal-title":"Biomaterials"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"274","DOI":"10.7150\/ijms.22644","article-title":"Risks of Using Sterilization by Gamma Radiation: The Other Side of the Coin","volume":"15","author":"Harrell","year":"2018","journal-title":"Int. J. Med. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"731","DOI":"10.3892\/etm.2010.136","article-title":"Gas plasma sterilization of microorganisms and mechanisms of action","volume":"1","author":"Shintani","year":"2010","journal-title":"Exp. Ther. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2517","DOI":"10.1039\/C3TB21453B","article-title":"The effect of residual endotoxin contamination on the neuroinflammatory response to sterilized intracortical microelectrodes","volume":"2","author":"Ravikumar","year":"2014","journal-title":"J. Mater. Chem. B"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1600331","DOI":"10.1002\/mame.201600331","article-title":"Sterilization of Thiol-ene\/Acrylate Based Shape Memory Polymers for Biomedical Applications","volume":"302","author":"Ecker","year":"2017","journal-title":"Macromol. Mater. Eng."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Bracco, G., and Holst, B. (2013). Chapter 1 Contact Angle and Wetting Properties. Surface Science Techniques, Springer Series in Surface Sciences, Springer.","DOI":"10.1007\/978-3-642-34243-1"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10856-015-5495-2","article-title":"The Evaluation of the Impact of Titania Nanotube Covers Morphology and Crystal Phase on Their Biological Properties","volume":"26","author":"Lewandowska","year":"2015","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1002\/app.1969.070130815","article-title":"Estimation of the surface free energy of polymers","volume":"13","author":"Owens","year":"1969","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1002\/jbm.a.30320","article-title":"High surface energy enhances cell response to titanium substrate microstructure","volume":"74","author":"Zhao","year":"2005","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_35","first-page":"61","article-title":"Biology of implant osseointegration","volume":"9","author":"Mavrogenis","year":"2009","journal-title":"J. Musculoskelet. Neuronal. Interact."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Radtke, A.T., J\u0119drzejewski, W., Kozak, B., Sadowska, M., Wi\u0119ckowska-Szakiel, E., Talik, M., M\u00e4kel\u00e4, M., and Leskel\u00e4, P. (2017). Optimization of the silver clusters PEALD process on the surface of 1-D titania coatings. Nanomaterials, 7.","DOI":"10.3390\/nano7070193"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Piszczek, P., Lewandowska, \u017b., Radtke, A., J\u0119drzejewski, T., Kozak, W., Sadowska, B., Szubka, M., Talik, E., and Fiori, F. (2017). Biocompatibility of Titania Nanotube Coatings Enriched with Silver Nanograins by Chemical Vapor Depositiom. Nanomaterials, 7.","DOI":"10.3390\/nano7090274"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.electacta.2012.06.006","article-title":"Water annealing and other low temperature treatments of anodic TiO2 nanotubes: A comparison of properties and efficiencies in dye sensitized solar cells and for water splitting","volume":"82","author":"Liu","year":"2012","journal-title":"Electrochim. Acta"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.bioelechem.2016.02.001","article-title":"Influence of various sterilization procedures on TiO2 nanotubes used for bimedical devices","volume":"109","author":"Junkar","year":"2016","journal-title":"Bioelectrochemistry"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"7808","DOI":"10.1038\/srep07808","article-title":"Ultrafast room-temperature crystallization of TiO2 Nanotubes exploiting water-vapor treatment","volume":"5","author":"Lamberti","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2800","DOI":"10.1021\/am200685s","article-title":"A facile method to crystallize amorphous anodized TiO2 nanotubes at low temperature","volume":"3","author":"Liao","year":"2011","journal-title":"Acs Appl. Mater. Interfaces"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6139","DOI":"10.1039\/c3nr01286g","article-title":"Low-temperature crystallization of anodized TiO2 nanotubes at the solid-gas interface and their photoelectrochemical properties","volume":"5","author":"Liu","year":"2013","journal-title":"Nanoscale"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4663","DOI":"10.1016\/S0142-9612(03)00366-1","article-title":"Characterization of surface oxide films on titanium and adhesion of osteoblast","volume":"24","author":"Feng","year":"2003","journal-title":"Biomaterials"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"209","DOI":"10.5185\/amlett.2016.6014","article-title":"Titanium dioxide nanotube arrays: A novel approach into periodontal tissue regeneration on the surface of titanium implants","volume":"7","author":"Goodarzi","year":"2016","journal-title":"Adv. Mater. Lett."},{"key":"ref_45","first-page":"36","article-title":"Titanium dioxide and modified titanium dioxide by silver nanoparticles as an anti-biofilm filler content for composite resins","volume":"35","author":"Bezerra","year":"2018","journal-title":"Dent. Mater."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3888","DOI":"10.1016\/j.actbio.2012.06.037","article-title":"Development of new metallic alloys for biomedical applications","volume":"8","author":"Niinomi","year":"2012","journal-title":"Acta Biomater."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.jmbbm.2012.11.014","article-title":"Biocompatibility of Ti-alloys for long-term implantation","volume":"20","author":"Niinomi","year":"2013","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.msea.2016.03.023","article-title":"Microstructure and mechanical properties of nanostructured and microstructured TiO2 films","volume":"661","author":"Dikici","year":"2016","journal-title":"Mater. Sci. Eng. A"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jelechem.2016.03.032","article-title":"Role of crystallinity on the nanomechanical and electrochemical properties of TiO2 nanotubes","volume":"770","author":"Munirathinam","year":"2016","journal-title":"J. Electroanal. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2655","DOI":"10.1016\/j.surfcoat.2011.11.010","article-title":"Nanoindentation study of the mechanical and damage behaviour of suspension plasma sprayed TiO2 coatings","volume":"206","author":"Bonache","year":"2012","journal-title":"Surf. Coat. Technol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.apsusc.2017.07.199","article-title":"Hybrid biocomposites based on titania nanotubes and a hydroxyapatite coating deposited by RF-magnetron sputtering: Surface topography, structure, and mechanical properties","volume":"426","author":"Chernozem","year":"2017","journal-title":"Appl. Surf. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1016\/j.surfcoat.2005.07.077","article-title":"Biomedical applications of diamond-like carbon (DLC) coatings: A review","volume":"200","author":"Dearnaley","year":"2005","journal-title":"Surf. Coat. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1080\/02670844.2017.1305657","article-title":"Properties of composite oxide layers on the Ti13Nb13Zr alloy","volume":"33","author":"Ossowska","year":"2017","journal-title":"Surf. Eng."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.msec.2016.12.046","article-title":"Modification of Ti6Al4V implant surfaces by biocompatible TiO2\/PCL hybrid layers prepared via sol-gel dip coating: Structural characterization, mechanical and corrosion behavior","volume":"74","author":"Catauro","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.phpro.2012.12.003","article-title":"Improvement of the adhesion between TiO2 nanofilm and glass substrate by roughness modifications","volume":"40","author":"Montorosi","year":"2013","journal-title":"Phys. Procedia"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s10266-011-0029-y","article-title":"The difference of fibroblast behavior on titanium substrata with different surface characteristics","volume":"100","author":"Furuhashi","year":"2012","journal-title":"Odontology"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.nantod.2010.01.001","article-title":"Influence of nanoscale surface topography on protein adsorption and cellular response","volume":"5","author":"Lord","year":"2010","journal-title":"Nano Today"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1083\/jcb.200508130","article-title":"Osteoblast differentiation and skeletal development are regulated by Mdm2-p53 signaling","volume":"172","author":"Lengner","year":"2006","journal-title":"J. Cell. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1002\/jbm.b.32979","article-title":"In vitro biocompatibility analysis of novel nano-biphasic calcium phosphate scaffolds in different composition ratios","volume":"102","author":"Ebrahimi","year":"2014","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/8\/2\/272\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:34:22Z","timestamp":1760186062000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/8\/2\/272"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,23]]},"references-count":59,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2]]}},"alternative-id":["jcm8020272"],"URL":"https:\/\/doi.org\/10.3390\/jcm8020272","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,23]]}}}