{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T15:15:58Z","timestamp":1790781358545,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,3,26]],"date-time":"2019-03-26T00:00:00Z","timestamp":1553558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["DK82370, DK82342, DK82315, DK82344, DK82325, DK82345, DK82333, and DK82316"],"award-info":[{"award-number":["DK82370, DK82342, DK82315, DK82344, DK82325, DK82345, DK82333, and DK82316"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Health (NIH) MAPP Network Awards","award":["DK82370, DK82342, DK82315, DK82344, DK82325, DK82345, DK82333, and DK82316"],"award-info":[{"award-number":["DK82370, DK82342, DK82315, DK82344, DK82325, DK82345, DK82333, and DK82316"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>We surveyed urine microbiota of females diagnosed with interstitial cystitis\/bladder pain syndrome (IC\/BPS) and matched control participants enrolled in the National Institutes of Health (NIH) Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network using the culture-independent methodology. Midstream urine specimens were analyzed with the Plex-ID molecular diagnostic platform that utilizes polymerase chain reaction\u2013electrospray ionization\u2013time-of-flight\u2013mass spectrometry (PCR-ESI-TOF MS) to provide a comprehensive identification of bacterial and select fungal species. IC\/BPS and control participants were evaluated for differences (presence, diversity, and abundance) in species and genus. Urine specimens obtained from 181 female IC\/BPS and 182 female control participants detected a total of 92 species (41 genera). Mean (SD) species count was 2.49 (1.48) and 2.30 (1.28) among IC\/BPS and control participants, respectively. Overall species composition did not significantly differ between IC\/BPS and control participants at any level (p = 0.726 species level, p = 0.222 genus level). IC\/BPS participants urine trended to an overabundance of Lactobacillus gasseri (p = 0.09) detected but had a lower prevalence of Corynebacterium compared with control participants (p = 0.002). The relative abundance data analysis mirrored the prevalence data differences with no significant differences in most species or genus abundance other than Lactobacillus gasseri and Corynebacterium (p = 0.08 and p = 0.001, respectively). No cause and\/or effect conclusion can be drawn from this observation, but it suggests that a more comprehensive evaluation (vaginal, bowel, catheterized bladder and\/or tissue-based specimens) of the lower urinary tract microbiota in IC\/BPS patients is warranted.<\/jats:p>","DOI":"10.3390\/jcm8030415","type":"journal-article","created":{"date-parts":[[2019,3,27]],"date-time":"2019-03-27T05:03:12Z","timestamp":1553662992000},"page":"415","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["A Culture-Independent Analysis of the Microbiota of Female Interstitial Cystitis\/Bladder Pain Syndrome Participants in the MAPP Research Network"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4865-7293","authenticated-orcid":false,"given":"J.","family":"Nickel","sequence":"first","affiliation":[{"name":"Department of Urology, Queen\u2019s University, Kingston, ON K0H 2T0, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisa","family":"Stephens-Shields","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8099-0988","authenticated-orcid":false,"given":"J.","family":"Landis","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Epidemiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Mullins","sequence":"additional","affiliation":[{"name":"National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrie","family":"van Bokhoven","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Lucia","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Henderson","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhaswati","family":"Sen","sequence":"additional","affiliation":[{"name":"Departments of Microbiology &amp; Immunology; Drexel University College of Medicine, Philadephia, PA 19102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaroslaw","family":"Krol","sequence":"additional","affiliation":[{"name":"Departments of Microbiology &amp; Immunology; Drexel University College of Medicine, Philadephia, PA 19102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garth","family":"Ehrlich","sequence":"additional","affiliation":[{"name":"Departments of Microbiology &amp; Immunology; Drexel University College of Medicine, Philadephia, PA 19102, USA"},{"name":"Department of Otolaryngology-Head and Neck Surgery, Drexel University College of Medicine, Philadelphia, PA 19102, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"The MAPP Research Network","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1016\/j.juro.2011.03.064","article-title":"AUA guideline for the diagnosis and treatment of interstitial cystitis\/bladder pain syndrome","volume":"185","author":"Hanno","year":"2011","journal-title":"J. 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