{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:37:14Z","timestamp":1790696234673,"version":"4.1.0"},"reference-count":133,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,8]],"date-time":"2020-02-08T00:00:00Z","timestamp":1581120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Saliva is a fascinating biological fluid which has all the features of a perfect diagnostic tool. In fact, its collection is rapid, simple, and noninvasive. Thanks to several transport mechanisms and its intimate contact with crevicular fluid, saliva contains hundreds of proteins deriving from plasma. Advances in analytical techniques have opened a new era\u2014called \u201csalivaomics\u201d\u2014that investigates the salivary proteome, transcriptome, microRNAs, metabolome, and microbiome. In recent years, researchers have tried to find salivary biomarkers for oral and systemic diseases with various protocols and technologies. The review aspires to provide an overall perspective of salivary biomarkers concerning oral diseases such as lichen planus, oral cancer, blistering diseases, and psoriasis. Saliva has proved to be a promising substrate for the early detection of oral diseases and the evaluation of therapeutic response. However, the wide variation in sampling, processing, and measuring of salivary elements still represents a limit for the application in clinical practice.<\/jats:p>","DOI":"10.3390\/jcm9020466","type":"journal-article","created":{"date-parts":[[2020,2,10]],"date-time":"2020-02-10T11:48:51Z","timestamp":1581335331000},"page":"466","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["Saliva and Oral Diseases"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0815-172X","authenticated-orcid":false,"given":"Emanuela","family":"Martina","sequence":"first","affiliation":[{"name":"Dermatology Clinic, Department of Clinical and Molecular Sciences, Polytechnic Marche University, via Conca 71, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3740-0839","authenticated-orcid":false,"given":"Anna","family":"Campanati","sequence":"additional","affiliation":[{"name":"Dermatology Clinic, Department of Clinical and Molecular Sciences, Polytechnic Marche University, via Conca 71, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Diotallevi","sequence":"additional","affiliation":[{"name":"Dermatology Clinic, Department of Clinical and Molecular Sciences, Polytechnic Marche University, via Conca 71, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annamaria","family":"Offidani","sequence":"additional","affiliation":[{"name":"Dermatology Clinic, Department of Clinical and Molecular Sciences, Polytechnic Marche University, via Conca 71, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/j.clindermatol.2017.06.005","article-title":"Oral mucosa biology and salivary biomarkers","volume":"35","author":"Qin","year":"2017","journal-title":"Clin. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000358776","article-title":"Anatomy, Biogenesis and Regeneration in Salivary Glands","volume":"24","author":"Holmberg","year":"2014","journal-title":"Monogr. Oral Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1111\/j.1365-2842.2007.01794.x","article-title":"Salivary secretion, taste and hyposalivation","volume":"34","author":"Mese","year":"2007","journal-title":"J. Oral Rehabil."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1177\/1535370216681550","article-title":"Saliva diagnostics\u2014Current views and directions","volume":"242","author":"Aro","year":"2017","journal-title":"Exp. Biol. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1373\/clinchem.2010.153767","article-title":"Diagnostic potential of saliva: Current state and future applications","volume":"57","author":"Pfaffe","year":"2011","journal-title":"Clin. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jobcr.2015.08.006","article-title":"Saliva as a diagnostic tool for oral and systemic diseases","volume":"6","author":"Javaid","year":"2016","journal-title":"J. Oral Biol. Craniofacial Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1016\/j.jpba.2017.07.009","article-title":"Two-dimensional liquid chromatography in pharmaceutical analysis. Instrumental aspects, trends and applications","volume":"145","author":"Iguiniz","year":"2017","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1016\/S1368-8375(03)00102-7","article-title":"Pooled estimate of world leukoplakia prevalence: A systematic review","volume":"39","author":"Petti","year":"2003","journal-title":"Oral Oncol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/odi.13041","article-title":"World Workshop on Oral Medicine VII: Biomarkers predicting lymphoma in the salivary glands of patients with Sj\u00f6gren\u2019s syndrome\u2014A systematic review","volume":"25","author":"Delli","year":"2019","journal-title":"Oral Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1002\/prca.201100046","article-title":"The salivary proteome: Challenges and perspectives","volume":"5","author":"Siqueira","year":"2011","journal-title":"PROTEOMICS Clin. Appl."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/j.1749-6632.1993.tb18343.x","article-title":"Methods for Collecting Saliva","volume":"694","author":"Navazesh","year":"1993","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_12","first-page":"6141845","article-title":"Protein-Based Salivary Profiles as Novel Biomarkers for Oral Diseases","volume":"2018","author":"Bravo","year":"2018","journal-title":"Dis. Markers"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1159\/000358781","article-title":"Salivary Secretion: Mechanism and Neural Regulation","volume":"Volume 24","author":"Proctor","year":"2014","journal-title":"Monographs in Oral Science"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Henson, B.S., and Wong, D.T. (2010). Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications. Oral Biology. Methods in Molecular Biology (Methods and Protocols), Humana Press.","DOI":"10.1007\/978-1-60761-820-1_2"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1177\/154411130201300209","article-title":"The diagnostic applications of saliva\u2014A review","volume":"13","author":"Kaufman","year":"2002","journal-title":"Crit. Rev. Oral Biol. Med."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Khurshid, Z., Zohaib, S., Najeeb, S., Zafar, M.S., Slowey, P.D., and Almas, K. (2016). Human Saliva Collection Devices for Proteomics: An Update. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17060846"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.jchromb.2006.10.005","article-title":"SELDI-TOF-MS of saliva: Methodology and pre-treatment effects","volume":"847","author":"Schipper","year":"2007","journal-title":"J. Chromatogr. B"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ai, J., Smith, B., and Wong, D.T. (2010). Saliva Ontology: An ontology-based framework for a Salivaomics Knowledge Base. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-302"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1158\/1055-9965.EPI-05-0706","article-title":"Quality and Quantity of Saliva DNA Obtained from the Self-administrated Oragene Method\u2014A Pilot Study on the Cohort of Swedish Men","volume":"15","author":"Tybring","year":"2006","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Abraham, J.E., Maranian, M.J., Spiteri, I., Russell, R., Ingle, S., Luccarini, C., Earl, H.M., Pharoah, P.P.D., Dunning, A.M., and Caldas, C. (2012). Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping. BMC Med. Genom., 5.","DOI":"10.1186\/1755-8794-5-19"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ga\u017adzicka, J., Go\u0142\u0105bek, K., Strzelczyk, J.K., and Ostrowska, Z. (2019). Epigenetic Modifications in Head and Neck Cancer. Biochem. Genet.","DOI":"10.1007\/s10528-019-09941-1"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1373\/clinchem.2010.159210","article-title":"Direct Saliva Transcriptome Analysis","volume":"57","author":"Lee","year":"2011","journal-title":"Clin. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"429","DOI":"10.2174\/138920101505140828161335","article-title":"Editorial (Thematic Issue: \u201cmiRNA and Cancer; Computational and Experimental Approaches\u201d)","volume":"15","author":"Tutar","year":"2014","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1111\/jcmm.13007","article-title":"Emerging technologies for salivaomics in cancer detection","volume":"21","author":"Kaczor","year":"2017","journal-title":"J. Cell. Mol. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"11945","DOI":"10.18632\/oncotarget.7858","article-title":"Anti-TNF-\u03b1 treatment modulates SASP and SASP-related microRNAs in endothelial cells and in circulating angiogenic cells","volume":"7","author":"Prattichizzo","year":"2016","journal-title":"Oncotarget"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"8676","DOI":"10.3390\/ijms16048676","article-title":"Non-coding RNAs in saliva: Emerging biomarkers for molecular diagnostics","volume":"16","author":"Majem","year":"2015","journal-title":"Int. J. Mol. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: Target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"R762","DOI":"10.1016\/j.cub.2014.06.043","article-title":"Aberrant regulation and function of microRNAs in cancer","volume":"24","author":"Adams","year":"2014","journal-title":"Curr. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"783","DOI":"10.4155\/bio.13.35","article-title":"Analysis of human saliva metabolome by direct immersion solid-phase microextraction LC and benchtop orbitrap MS","volume":"5","author":"Bessonneau","year":"2013","journal-title":"Bioanalysis"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1007\/s11306-009-0178-y","article-title":"Capillary electrophoresis mass spectrometry-based saliva metabolomics identified oral, breast and pancreatic cancer-specific profiles","volume":"6","author":"Sugimoto","year":"2010","journal-title":"Metabolomics"},{"key":"ref_31","first-page":"6795","article-title":"Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer","volume":"16","author":"Mikkonen","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_32","unstructured":"Bjerrum, J.T. (2015). The Strengths and Weaknesses of NMR Spectroscopy and Mass Spectrometry with Particular Focus on Metabolomics Research. Metabonomics: Methods and Protocols, Springer."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.chroma.2019.04.071","article-title":"Rapid liquid chromatography tandem mass spectrometry method for targeted quantitation of human performance metabolites in saliva","volume":"1601","author":"McBride","year":"2019","journal-title":"J. Chromatogr. A"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Beale, D.J., Jones, O.A.H., Karpe, A.V., Dayalan, S., Oh, D.Y., Kouremenos, K.A., Ahmed, W., and Palombo, E.A. (2017). A review of analytical techniques and their application in disease diagnosis in breathomics and salivaomics research. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18010024"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Esteves, C.V., de Campos, W.G., de Souza, M.M., Louren\u00e7o, S.V., Siqueira, W.L., and Lemos-J\u00fanior, C.A. (2019). Diagnostic potential of saliva proteome analysis: A review and guide to clinical practice. Braz. Oral Res., 33.","DOI":"10.1590\/1807-3107bor-2019.vol33.0043"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.aca.2012.02.017","article-title":"Method development for proteome stabilization in human saliva","volume":"722","author":"Xiao","year":"2012","journal-title":"Anal. Chim. Acta"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.bios.2016.02.040","article-title":"Recent advances in salivary cancer diagnostics enabled by biosensors and bioelectronics","volume":"81","author":"Mishra","year":"2016","journal-title":"Biosens. Bioelectron."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.bbapap.2018.07.002","article-title":"Cancer biomarker development and two-dimensional difference gel electrophoresis (2D-DIGE)","volume":"1867","author":"Kondo","year":"2019","journal-title":"Biochim. Biophys. Acta Proteins Proteom."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Keohavong, P., Singh, K.P., and Gao, W. (2020). Fast and Efficient Measurement of Clinical and Biological Samples Using Immunoassay-Based Multiplexing Systems. Molecular Toxicology Protocols, Springer.","DOI":"10.1007\/978-1-0716-0223-2"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Hnasko, R. (2015). V Direct ELISA. ELISA: Methods and Protocols, Springer.","DOI":"10.1007\/978-1-4939-2742-5"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1038\/ismej.2016.199","article-title":"On the ecosystemic network of saliva in healthy young adults","volume":"11","author":"Zaura","year":"2017","journal-title":"ISME J."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Ge, X., Rodriguez, R., Trinh, M., Gunsolley, J., and Xu, P. (2013). Oral microbiome of deep and shallow dental pockets in chronic periodontitis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065520"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1177\/0022034512449462","article-title":"Progress dissecting the oral microbiome in caries and health","volume":"24","author":"Burne","year":"2012","journal-title":"Adv. Dent. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1038\/nrc3610","article-title":"The microbiome and cancer","volume":"13","author":"Schwabe","year":"2013","journal-title":"Nat. Rev. Cancer"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e1373","DOI":"10.7717\/peerj.1373","article-title":"Characterization of the salivary microbiome in patients with pancreatic cancer","volume":"3","author":"Torres","year":"2015","journal-title":"PeerJ"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.5740\/jaoacint.17-0207","article-title":"Identification of microorganisms by modern analytical techniques","volume":"100","author":"Buszewski","year":"2017","journal-title":"J. AOAC Int."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1007\/s40257-019-00461-7","article-title":"Oral Lesions in Autoimmune Bullous Diseases: An Overview of Clinical Characteristics and Diagnostic Algorithm","volume":"20","author":"Rashid","year":"2019","journal-title":"Am. J. Clin. Dermatol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/eos.12536","article-title":"Current concepts on Sj\u00f6gren\u2019s syndrome\u2013classification criteria and biomarkers","volume":"126","author":"Jonsson","year":"2018","journal-title":"Eur. J. Oral Sci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"104550","DOI":"10.1016\/j.oraloncology.2019.104550","article-title":"Oral potentially malignant disorders: A comprehensive review on clinical aspects and management","volume":"102","author":"Warnakulasuriya","year":"2020","journal-title":"Oral Oncol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/j.1600-0714.2008.00662.x","article-title":"The reported prevalence of oral lichen planus: A review and critique","volume":"37","author":"McCartan","year":"2008","journal-title":"J. Oral Pathol. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1600-0714.1988.tb01527.x","article-title":"Course of various clinical forms of oral lichen planus. A prospective follow-up study of 611 patients","volume":"17","author":"Thorn","year":"1988","journal-title":"J. Oral Pathol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1111\/j.1365-4632.2008.03654.x","article-title":"Lichen striatus in adults and pimecrolimus: Open, off-label clinical study","volume":"47","author":"Campanati","year":"2008","journal-title":"Int. J. Dermatol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.oooo.2016.05.004","article-title":"Diagnosis of oral lichen planus: A position paper of the American Academy of Oral and Maxillofacial Pathology","volume":"122","author":"Cheng","year":"2016","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol."},{"key":"ref_54","first-page":"ZC34","article-title":"Direct Immunofluorescence in Oral Lichen Planus","volume":"9","author":"Buajeeb","year":"2015","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.tripleo.2004.06.077","article-title":"Current controversies in oral lichen planus: Report of an international consensus meeting. Part 1. Viral infections and etiopathogenesis","volume":"100","author":"Lodi","year":"2005","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/S1079-2104(03)00305-6","article-title":"The possible premalignant character of oral lichen planus and oral lichenoid lesions: A prospective study","volume":"96","author":"Schepman","year":"2003","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol."},{"key":"ref_57","first-page":"e20170146","article-title":"Psychopathological profile and quality of life of patients with oral lichen planus","volume":"26","author":"Zwyrtek","year":"2018","journal-title":"J. Appl. Oral Sci."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Talungchit, S., Buajeeb, W., Lerdtripop, C., Surarit, R., Chairatvit, K., Roytrakul, S., Kobayashi, H., Izumi, Y., and Khovidhunkit, S.-o.P. (2018). Putative salivary protein biomarkers for the diagnosis of oral lichen planus: A case-control study. BMC Oral Health, 18.","DOI":"10.1186\/s12903-018-0504-8"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1111\/odi.12837","article-title":"Salivary proteomics in lichen planus: A relationship with pathogenesis?","volume":"24","author":"Souza","year":"2018","journal-title":"Oral Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"485","DOI":"10.2340\/00015555-2306","article-title":"Up-regulation of Proinflammatory Genes and Cytokines Induced by S100A8 in CD8 + T Cells in Lichen Planus","volume":"96","author":"Domingues","year":"2016","journal-title":"Acta Derm. Venereol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1007\/s12011-019-01702-w","article-title":"Role of Zinc in Zinc-\u03b12-Glycoprotein Metabolism in Obesity: A Review of Literature","volume":"193","author":"Severo","year":"2020","journal-title":"Biol. Trace Elem. Res."},{"key":"ref_62","first-page":"885","article-title":"Salivary oxidative status in patients with oral lichen planus","volume":"67","author":"Darczuk","year":"2016","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"58","DOI":"10.4068\/cmj.2014.50.2.58","article-title":"Levels of salivary antioxidant vitamins and lipid peroxidation in patients with oral lichen planus and healthy individuals","volume":"50","author":"Abdolsamadi","year":"2014","journal-title":"Chonnam Med. J."},{"key":"ref_64","first-page":"e459","article-title":"Topical treatment of oral lichen planus with anthocyanins","volume":"19","author":"Lanfranchi","year":"2014","journal-title":"Med. Oral Patol. Oral Cir. Bucal"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Skrinjar, I., Vidranski, V., Brzak, B.L., Juras, D.V., Rogulj, A.A., Brailo, V., and Boras, V.V. (2019). Salivary cortisol levels in patients with oral lichen planus\u2014A pilot case-control study. Dent. J., 7.","DOI":"10.3390\/dj7020059"},{"key":"ref_66","first-page":"19","article-title":"Salivary Cortisol and Salivary Flow Rate in Clinical Types of Oral Lichen Planus","volume":"16","author":"Mansourian","year":"2018","journal-title":"Skinmed"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Lopez-Jornet, P., Zavattaro, E., Mozaffari, H.R., Ramezani, M., and Sadeghi, M. (2019). Evaluation of the salivary level of cortisol in patients with oral lichen planus: A meta-analysis. Medicina, 55.","DOI":"10.3390\/medicina55050213"},{"key":"ref_68","first-page":"ZC01","article-title":"Association of salivary cortisol and anxiety levels in lichen planus patients","volume":"8","author":"Nadendla","year":"2014","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1034\/j.1600-0714.2001.300501.x","article-title":"Erosive oral lichen planus and salivary cortisol","volume":"30","author":"Jontell","year":"2001","journal-title":"J. Oral Pathol. Med."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1034\/j.1601-0825.2003.00960.x","article-title":"The evaluation of anxiety and salivary cortisol levels in patients with oral lichen planus","volume":"9","author":"Koray","year":"2003","journal-title":"Oral Dis."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"A271","DOI":"10.1016\/j.autrev.2009.11.014","article-title":"Stress and autoimmunity","volume":"9","author":"Stojanovich","year":"2010","journal-title":"Autoimmun. Rev."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.oooo.2018.02.013","article-title":"Mixed and inhomogeneous expression profile of Th1\/Th2 related cytokines detected by cytometric bead array in the saliva of patients with oral lichen planus","volume":"126","author":"Wei","year":"2018","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1158\/0008-5472.CAN-04-3589","article-title":"Interleukin 6 Supports the Maintenance of p53 Tumor Suppressor Gene Promoter Methylation","volume":"65","author":"Hodge","year":"2005","journal-title":"Cancer Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"103","DOI":"10.5114\/ceji.2018.74880","article-title":"Interleukin-6 levels in the serum and saliva of patients with oral lichen planus compared with healthy controls: A meta-analysis study","volume":"43","author":"Mozaffari","year":"2018","journal-title":"Cent. Eur. J. Immunol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0014-5793(92)80909-Z","article-title":"Interleukin-8, a chemotactic and inflammatory cytokine","volume":"307","author":"Baggiolini","year":"1992","journal-title":"FEBS Lett."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"599","DOI":"10.5114\/ada.2018.77611","article-title":"A systematic review and meta-analysis study of salivary and serum interleukin-8 levels in oral lichen planus","volume":"35","author":"Mozaffari","year":"2018","journal-title":"Postep. Dermatol. Alergol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1002\/mc.20113","article-title":"The feasibility of monitoring NF-\u03baB associated cytokines: TNF-\u03b1, IL-1\u03b1, IL-6, and IL-8 in whole saliva for the malignant transformation of oral lichen planus","volume":"44","author":"Rhodus","year":"2005","journal-title":"Mol. Carcinog."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1111\/j.1601-0825.2005.01165.x","article-title":"Proinflammatory cytokine levels in saliva before and after treatment of (erosive) oral lichen planus with dexamethasone","volume":"12","author":"Rhodus","year":"2006","journal-title":"Oral Dis."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.oraloncology.2008.06.002","article-title":"Global epidemiology of oral and oropharyngeal cancer","volume":"45","author":"Warnakulasuriya","year":"2009","journal-title":"Oral Oncol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"e767","DOI":"10.1016\/S1470-2045(17)30460-6","article-title":"Defining a global research and policy agenda for betel quid and areca nut","volume":"18","author":"Mehrtash","year":"2017","journal-title":"Lancet Oncol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1159\/000485322","article-title":"High-Risk Human Papillomavirus in Oral Cancer: Clinical Implications","volume":"94","author":"Yete","year":"2018","journal-title":"Oncology"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1016\/S1368-8375(03)00006-X","article-title":"Is there a natural limit of the transformation rate of oral leukoplakia?","volume":"39","author":"Scheifele","year":"2003","journal-title":"Oral Oncol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1159\/000063887","article-title":"General Practitioners\u2019 Accuracy in Diagnosing Skin Cancers","volume":"205","author":"Offidani","year":"2002","journal-title":"Dermatology"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1097\/00008469-200108000-00014","article-title":"Oral and pharyngeal cancer: Lack of prevention and early detection by health care providers","volume":"10","author":"Mignogna","year":"2001","journal-title":"Eur. J. Cancer Prev."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/s13402-018-0372-x","article-title":"Diagnostic and prognostic relevance of salivary microRNA-21, -125a, -31 and -200a levels in patients with oral lichen planus\u2014A short report","volume":"41","author":"Mehdipour","year":"2018","journal-title":"Cell. Oncol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"5473","DOI":"10.1158\/1078-0432.CCR-09-0736","article-title":"Salivary microRNA: Discovery, characterization, and clinical utility for oral cancer detection","volume":"15","author":"Park","year":"2009","journal-title":"Clin. Cancer Res."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1756-8722-6-6","article-title":"Diverse functions of miR-125 family in different cell contexts","volume":"6","author":"Sun","year":"2013","journal-title":"J. Hematol. Oncol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/odi.13209","article-title":"Discrimination of oral squamous cell carcinoma from oral lichen planus by salivary metabolomics","volume":"26","author":"Ishikawa","year":"2020","journal-title":"Oral Dis."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"158R","DOI":"10.1093\/glycob\/cwl040","article-title":"Fucosylation in prokaryotes and eukaryotes","volume":"16","author":"Ma","year":"2006","journal-title":"Glycobiology"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1093\/jb\/mvn011","article-title":"Biological Function of Fucosylation in Cancer Biology","volume":"143","author":"Miyoshi","year":"2008","journal-title":"J. Biochem."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"288","DOI":"10.4103\/0970-9290.44529","article-title":"Evaluation of salivary sialic acid, total protein, and total sugar in oral cancer: A preliminary report","volume":"19","author":"Sanjay","year":"2008","journal-title":"Indian J. Dent. Res."},{"key":"ref_92","first-page":"343","article-title":"Total sialic acid, total protein and total sugar levels in serum and saliva of oral squamous cell carcinoma patients: A case control study","volume":"10","author":"Dhakar","year":"2013","journal-title":"Dent. Res. J."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"214","DOI":"10.4103\/0019-509X.146720","article-title":"Serum and salivary sialic acid as a biomarker in oral potentially malignant disorders and oral cancer","volume":"51","author":"Dadhich","year":"2014","journal-title":"Indian J. Cancer"},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Panda, M., Rai, A.K., Rahman, T., Das, A., Das, R., Sarma, A., Kataki, A.C., and Chattopadhyay, I. (2019). Alterations of salivary microbial community associated with oropharyngeal and hypopharyngeal squamous cell carcinoma patients. Arch. Microbiol.","DOI":"10.1007\/s00203-019-01790-1"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.oraloncology.2018.12.003","article-title":"The microbiome and oral cancer: More questions than answers","volume":"89","author":"Healy","year":"2019","journal-title":"Oral Oncol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.oooo.2013.02.009","article-title":"Acetaldehyde production and microbial colonization in oral squamous cell carcinoma and oral lichenoid disease","volume":"116","author":"Marttila","year":"2013","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1016\/j.oooo.2015.01.019","article-title":"Site-specific acetaldehyde production and microbial colonization in relation to oral squamous cell carcinoma and oral lichenoid disease","volume":"119","author":"Marttila","year":"2015","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1111\/odi.12565","article-title":"Candida virulence and ethanol-derived acetaldehyde production in oral cancer and non-cancer subjects","volume":"22","author":"Alnuaimi","year":"2016","journal-title":"Oral Dis."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1111\/odi.12341","article-title":"Acetaldehyde production by major oral microbes","volume":"21","author":"Moritani","year":"2015","journal-title":"Oral Dis."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1038\/ismej.2016.37","article-title":"Cigarette smoking and the oral microbiome in a large study of American adults","volume":"10","author":"Wu","year":"2016","journal-title":"ISME J."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1159\/000478078","article-title":"Bullous Diseases","volume":"Volume 53","author":"Corbaux","year":"2017","journal-title":"Current Problems in Dermatology"},{"key":"ref_102","unstructured":"Murrell, D.F., Pe\u00f1a, S., Joly, P., Marinovic, B., Hashimoto, T., Diaz, L.A., Sinha, A.A., Payne, A.S., Daneshpazhooh, M., and Eming, R. (2019). Diagnosis and Management of Pemphigus: Recommendations by an International Panel of Experts. J. Am. Acad. Dermatol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/j.1600-0722.2006.00394.x","article-title":"Detection of pemphigus desmoglein 1 and desmoglein 3 autoantibodies and pemphigoid BP180 autoantibodies in saliva and comparison with serum values","volume":"114","author":"Andreadis","year":"2006","journal-title":"Eur. J. Oral Sci."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1111\/bjd.14410","article-title":"Serum and salivary IgG and IgA antibodies to desmoglein 3 in mucosal pemphigus vulgaris","volume":"175","author":"Ali","year":"2016","journal-title":"Br. J. Dermatol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/j.1468-3083.2009.03408.x","article-title":"Serum and salivary desmoglein 1 and 3 enzyme-linked immunosorbent assay in pemphigus vulgaris: Correlation with phenotype and severity","volume":"24","author":"Hallaji","year":"2010","journal-title":"J. Eur. Acad. Dermatology Venereol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1111\/ced.13124","article-title":"Correlation between salivary and serum anti-desmoglein 1 and 3 antibody titres using ELISA and between anti-desmoglein levels and disease severity in pemphigus vulgaris","volume":"42","author":"De","year":"2017","journal-title":"Clin. Exp. Dermatol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"97","DOI":"10.21873\/invivo.11030","article-title":"Salivary samples for the diagnosis of Pemphigus vulgaris using the BIOCHIP approach: A pilot study","volume":"31","author":"Russo","year":"2017","journal-title":"In Vivo"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"16047","DOI":"10.1038\/nrdp.2016.47","article-title":"Sj\u00f6gren syndrome","volume":"2","author":"Baldini","year":"2016","journal-title":"Nat. Rev. Dis. Prim."},{"key":"ref_109","first-page":"33","article-title":"Sj\u00f6gren\u2019s syndrome and systemic lupus erythematosus: Links and risks","volume":"11","author":"Pasoto","year":"2019","journal-title":"Open Access Rheumatol. Res. Rev."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Fern\u00e1ndez-Mart\u00ednez, G., Zamora-Legoff, V., and Hern\u00e1ndez Molina, G. (2019). Oral health-related quality of life in primary Sj\u00f6gren\u2019s syndrome. Reumatol. Cl\u00ednica (Engl. Ed.).","DOI":"10.1016\/j.reumae.2018.04.001"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/BF02231773","article-title":"Anti-SSA\/Ro and anti-SSB\/La antibodies in serum and saliva of patients with Sjogren\u2019s syndrome","volume":"12","author":"Fischel","year":"1993","journal-title":"Clin Rheumatol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1177\/0022034510390811","article-title":"Salivary anti-Ro60 and anti-Ro52 antibody profiles to diagnose Sjogren\u2019s Syndrome","volume":"90","author":"Ching","year":"2011","journal-title":"J. Dent. Res."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1002\/pmic.201000206","article-title":"Identification of autoantibody biomarkers for primary Sj\u00f6gren\u2019s syndrome using protein microarrays","volume":"11","author":"Hu","year":"2011","journal-title":"Proteomics"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1111\/odi.12252","article-title":"Saliva as a potential tool for diagnosis of dry mouth including Sj\u00f6gren\u2019s syndrome","volume":"21","author":"Ohyama","year":"2015","journal-title":"Oral Dis."},{"key":"ref_115","first-page":"970","article-title":"Salivary cytokine profiles in primary Sj\u00f6gren\u2019s syndrome differ from those in non-Sj\u00f6gren sicca in terms of TNF-\u03b1 levels and Th-1\/Th-2 ratios","volume":"29","author":"Kang","year":"2011","journal-title":"Clin. Exp. Rheumatol."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.rdc.2016.03.004","article-title":"The Proteomics of Saliva in Sj\u00f6gren\u2019s Syndrome","volume":"42","author":"Katsiougiannis","year":"2016","journal-title":"Rheum. Dis. Clin. N. Am."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jaut.2019.03.008","article-title":"Soluble siglec-5 is a novel salivary biomarker for primary Sjogren\u2019s syndrome","volume":"100","author":"Lee","year":"2019","journal-title":"J. Autoimmun."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1601-0825.2011.01849.x","article-title":"Deep sequencing of short RNAs reveals novel microRNAs in minor salivary glands of patients with Sj\u00f6gren\u2019s syndrome","volume":"18","author":"Tandon","year":"2012","journal-title":"Oral Dis."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1002\/eji.201040757","article-title":"Altered miR-146a expression in Sj\u00f6gren\u2019s syndrome and its functional role in innate immunity","volume":"41","author":"Pauley","year":"2011","journal-title":"Eur. J. Immunol."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1002\/art.30131","article-title":"MicroRNA expression profiles as biomarkers of minor salivary gland inflammation and dysfunction in Sj\u00f6gren\u2019s syndrome","volume":"63","author":"Alevizos","year":"2011","journal-title":"Arthritis Rheum."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.3389\/fgene.2019.01104","article-title":"Epigenetic Regulation in the Pathogenesis of Sj\u00f6gren Syndrome and Rheumatoid Arthritis","volume":"10","year":"2019","journal-title":"Front. Genet."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"71","DOI":"10.3389\/fgene.2014.00071","article-title":"The contribution of epigenetics in Sjogren\u2019s Syndrome","volume":"5","author":"Konsta","year":"2014","journal-title":"Front. Genet."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.jaut.2013.02.002","article-title":"Epigenetic dysregulation in salivary glands from patients with primary Sj\u00f6gren\u2019s syndrome may be ascribed to infiltrating B cells","volume":"41","author":"Thabet","year":"2013","journal-title":"J. Autoimmun."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1186\/1479-5876-9-188","article-title":"Correspondence between salivary proteomic pattern and clinical course in primary Sj\u00f6gren syndrome and non-Hodgkin\u2019s lymphoma: A case report","volume":"9","author":"Baldini","year":"2011","journal-title":"J. Transl. Med."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"30039","DOI":"10.18632\/oncotarget.15613","article-title":"Investigation of three potential autoantibodies in Sjogren\u2019s syndrome and associated MALT lymphoma","volume":"8","author":"Cui","year":"2017","journal-title":"Oncotarget"},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Sharma, D., Sandhya, P., Vellarikkal, S.K., Surin, A.K., Jayarajan, R., Verma, A., Kumar, A., Ravi, R., Danda, D., and Sivasubbu, S. (2019). Saliva microbiome in primary Sj\u00f6gren\u2019s syndrome reveals distinct set of disease-associated microbes. Oral Dis.","DOI":"10.1111\/odi.13191"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"31119","DOI":"10.3402\/jom.v8.31119","article-title":"Microbiological and bioinformatics analysis of primary Sj\u00f6gren\u2019s syndrome patients with normal salivation","volume":"8","author":"Siddiqui","year":"2016","journal-title":"J. Oral Microbiol."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1038\/cmi.2018.7","article-title":"The microbiome and autoimmunity: A paradigm from the gut\u2013liver axis","volume":"15","author":"Li","year":"2018","journal-title":"Cell. Mol. Immunol."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/ijd.12706","article-title":"Serum levels of adipocytokines in psoriasis patients receiving tumor necrosis factor-\u03b1 inhibitors: results of a retrospective analysis","volume":"54","author":"Campanati","year":"2015","journal-title":"Int. J. Dermatol."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1111\/jdv.13023","article-title":"Homocysteine plasma levels in psoriasis patients: our experience and review of the literature","volume":"29","author":"Campanati","year":"2015","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"S56","DOI":"10.1002\/ddr.21194","article-title":"Periodontal disease: an oral manifestation of psoriasis or an occasional finding?","volume":"75","author":"Ganzetti","year":"2014","journal-title":"Drug Dev. Res."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1111\/bjd.13201","article-title":"Involvement of the oral cavity in psoriasis: Results of a clinical study","volume":"172","author":"Ganzetti","year":"2015","journal-title":"Br. J. Dermatol."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1177\/0300060515598902","article-title":"Salivary interleukin-1\u03b2: Oral inflammatory biomarker in patients with psoriasis","volume":"44","author":"Ganzetti","year":"2016","journal-title":"J. Int. Med. Res."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/9\/2\/466\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:55:56Z","timestamp":1760172956000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/9\/2\/466"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,8]]},"references-count":133,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["jcm9020466"],"URL":"https:\/\/doi.org\/10.3390\/jcm9020466","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,8]]}}}