{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:00:45Z","timestamp":1792112445194,"version":"4.4.0"},"reference-count":39,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T00:00:00Z","timestamp":1590364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Clinical evidence indicates that innate immune cells may contribute to acute coronary syndrome (ACS). Our prospective study aimed at investigating the association of neutrophil phenotypes with ACS. 108 patients were categorized into chronic stable coronary artery disease (n = 37), unstable angina (UA) (n = 19), Non-ST-Elevation Myocardial Infarction (NSTEMI) (n = 25), and ST-Elevation Myocardial Infarction (STEMI) (n = 27). At the time of inclusion, blood neutrophil subpopulations were analysed by flow cytometry. Differential blood cell count and plasma levels of neutrophilic soluble markers were recorded at admission and, for half of patients, at six-month follow-up. STEMI and NSTEMI patients displayed higher neutrophil count and neutrophil-to-lymphocyte ratio than stable and UA patients (p &lt; 0.0001), which normalized at six-month post-MI. Atypical low-density neutrophils were detected in the blood of the four patient groups. STEMI patients were characterized by elevated percentages of band cells compared to the other patients (p = 0.019). Multivariable logistic regression analysis revealed that plasma levels of total myeloperoxidase was associated with STEMI compared to stable (OR: 1.434; 95% CI: 1.119\u20131.837; P &lt; 0.0001), UA (1.47; 1.146\u20131.886; p = 0.002), and NSTEMI (1.213; 1.1\u20131.134; p = 0.0001) patients, while increased neutrophil side scatter (SSC) signal intensity was associated with NSTEMI compared to stable patients (3.828; 1.033\u201314.184; p = 0.045). Hence, changes in neutrophil phenotype are concomitant to ACS.<\/jats:p>","DOI":"10.3390\/jcm9051602","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T11:42:02Z","timestamp":1590406922000},"page":"1602","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Neutrophil Phenotypes in Coronary Artery Disease"],"prefix":"10.3390","volume":"9","author":[{"given":"Patrick","family":"Mar\u00e9chal","sequence":"first","affiliation":[{"name":"Department of Cardiology, University of Li\u00e8ge Hospital, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4255-0786","authenticated-orcid":false,"given":"Julien","family":"Tridetti","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University of Li\u00e8ge Hospital, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai-Linh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University of Li\u00e8ge Hospital, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odile","family":"W\u00e9ra","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiology, GIGA Cardiovascular Sciences, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4914-7041","authenticated-orcid":false,"given":"Zheshen","family":"Jiang","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiology, GIGA Cardiovascular Sciences, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Gustin","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiology, GIGA Cardiovascular Sciences, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Fran\u00e7oise","family":"Donneau","sequence":"additional","affiliation":[{"name":"Biostatistics Unit, Department of Public Health, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7561-0132","authenticated-orcid":false,"given":"C\u00e9cile","family":"Oury","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiology, GIGA Cardiovascular Sciences, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizio","family":"Lancellotti","sequence":"additional","affiliation":[{"name":"Department of Cardiology, University of Li\u00e8ge Hospital, 4000 Li\u00e8ge, Belgium"},{"name":"Laboratory of Cardiology, GIGA Cardiovascular Sciences, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"},{"name":"Gruppo Villa Maria Care and Research, Anthea Hospital, 70123 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1161\/CIRCULATIONAHA.118.035107","article-title":"Low-grade inflammation modifies cardiovascular risk even at very low ldl-c levels: Are we aiming for a dual target concept?","volume":"138","author":"Koenig","year":"2018","journal-title":"Circulation"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1001\/jamacardio.2019.0179","article-title":"Association of initial and serial c-reactive protein levels with adverse cardiovascular events and death after acute coronary syndrome: A secondary analysis of the vista-16 trial","volume":"4","author":"Mani","year":"2019","journal-title":"JAMA Cardiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1056\/NEJMoa1707914","article-title":"Antiinflammatory therapy with canakinumab for atherosclerotic disease","volume":"377","author":"Ridker","year":"2017","journal-title":"N. 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