{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:44:45Z","timestamp":1790840685383,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T00:00:00Z","timestamp":1591228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>One thousand and eighty patients, having prolonged bleeding times, frequent epistaxis, menorrhagia or easy bruising or other bleeding manifestations, and excluding those with von Willebrand\u2019s disease, were evaluated for platelet dense granule deficiency. The mean diameter of platelet dense granules was determined for all patients using image analysis. Four hundred and ninety-nine had \u201cclassic\u201d dense (delta) granule storage pool deficiency (\u03b4-SPD). Five hundred and eighty-one individuals (53.8%) were found to have a normal mean number of dense granules, but for some of these patients, the dense granules were smaller than for the controls. Of the patients having a normal number of dense granules, 165 (28.4%) were found to have significantly smaller granules than the platelets obtained from the control subjects. Their average granule diameter was 123.35 \u00b1 0.86 nm, that is more than three standard deviations below the mean of the control data. Total \u03b4-granule storage pool volumes (TDGV)\/platelet were calculated using these measurements. Individuals with \u03b4-SPD had half the number of granules (2.25 \u00b1 0.04 DG\/PL) and storage pool volume (3.88 \u00b1 1.06 \u00d7 106 nm3) when compared to our control data (4.64 \u00b1 0.11 DG\/PL; 10.79 \u00d7 106 nm3 \u00b1 0.42). Individuals having a bleeding history but a normal average of small dense granules had a calculated storage pool volume statistically different than controls and essentially the same storage pool volume as patients with \u03b4-SPD. We have identified a sub-classification of \u03b4-SPD that we have defined as micro-granular storage pool deficiency (\u03b4-MGSPD).<\/jats:p>","DOI":"10.3390\/jcm9061734","type":"journal-article","created":{"date-parts":[[2020,6,5]],"date-time":"2020-06-05T03:32:21Z","timestamp":1591327941000},"page":"1734","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A Morphometric Analysis of Platelet Dense Granules of Patients with Unexplained Bleeding: A New Entity of Delta-Microgranular Storage Pool Deficiency"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4190-2479","authenticated-orcid":false,"suffix":"III","given":"William T.","family":"Gunning","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Ohio, Toledo, OH 43614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meera","family":"Raghavan","sequence":"additional","affiliation":[{"name":"Kansas City University of Medicine and Biosciences, Kansas City, MO 64106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6664-0479","authenticated-orcid":false,"given":"Edward P.","family":"Calomeni","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Ohio, Toledo, OH 43614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James N.","family":"Turner","sequence":"additional","affiliation":[{"name":"New York State Department of Health, Albany, NY 12203, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bodri","family":"Roysam","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering, University of Houston, Houston, TX 77204, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantala","family":"Roysam","sequence":"additional","affiliation":[{"name":"Independent Researcher, Houston, TX 77002, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary R.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Toledo, Toledo, OH 43614, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Kouides","sequence":"additional","affiliation":[{"name":"Mary Gooley Hemophilia Center, Rochester General Hospital, Rochester, NY 14621, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4637-4211","authenticated-orcid":false,"given":"Neil A.","family":"Lachant","sequence":"additional","affiliation":[{"name":"Wilmot Cancer Institute, University of Rochester Medical Center, Rochester, NY 14642, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/j.1365-2141.1985.tb07334.x","article-title":"The storage pool deficiency in platelets from humans with the Chediak-Higashi syndrome: Study of six patients","volume":"59","author":"Cruz","year":"1985","journal-title":"Br. 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