{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:24:26Z","timestamp":1790745866756,"version":"4.1.0"},"reference-count":38,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T00:00:00Z","timestamp":1594080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Systemic sclerosis (SSc) is a systemic disease characterized by a great clinical and immunological heterogeneity whose pathophysiology is still being unraveled. Recently, innate immunity has been proposed to participate to the pathogenesis of SSc. In this study, we investigated the release of neutrophil extracellular traps (NETs) according to patient phenotype. Polymorphonuclear neutrophils (PMN) from 34 SSc patients and 26 healthy controls were stimulated by serum from SSc or healthy subject. NETs were visualized using epifluorescence microscope after DNA, myeloperoxidase, and Histone H3 tagging. Area of NETs were quantified using an original macro running in ImageJ\u00ae software. PMN from SSc patients were significantly more prone to releasing NETs than control PMN after autologous stimulation. PMN from patients with severe vascular complications (pulmonary arterial hypertension, digital ulcers) produced more NETs than PMN from other SSc patients and their aberrant NET production appeared to be sustained over time. In patients with pulmonary interstitial disease or extensive cutaneous fibrosis, NET production was high at an early stage of the disease before progressively decreasing. Both serum factors and PMN activation status were involved in the enhanced production of NETs in SSc. Consequently, neutrophils and especially NETosis represent new physiopathological and therapeutic fields in SSc.<\/jats:p>","DOI":"10.3390\/jcm9072136","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T10:41:09Z","timestamp":1594118469000},"page":"2136","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Neutrophil Extracellular Traps Generation Relates with Early Stage and Vascular Complications in Systemic Sclerosis"],"prefix":"10.3390","volume":"9","author":[{"given":"Kevin","family":"Didier","sequence":"first","affiliation":[{"name":"EA7509 IRMAIC, University of Reims-Champagne-Ardenne, 51100 Reims, France"},{"name":"Department of Internal Medicine, Infectious Diseases, and Clinical Immunology, Reims Teaching Hospitals, Robert Debr\u00e9 Hospital, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Giusti","sequence":"additional","affiliation":[{"name":"EA7509 IRMAIC, University of Reims-Champagne-Ardenne, 51100 Reims, France"},{"name":"Laboratory of Immunology, Reims University Hospital, University of Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4027-2855","authenticated-orcid":false,"given":"Sebastien","family":"Le Jan","sequence":"additional","affiliation":[{"name":"EA7509 IRMAIC, University of Reims-Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Terryn","sequence":"additional","affiliation":[{"name":"PICT Platform, University of Reims Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine","family":"Muller","sequence":"additional","affiliation":[{"name":"EA7509 IRMAIC, University of Reims-Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bach Nga","family":"Pham","sequence":"additional","affiliation":[{"name":"EA7509 IRMAIC, University of Reims-Champagne-Ardenne, 51100 Reims, France"},{"name":"Laboratory of Immunology, Reims University Hospital, University of Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Le Naour","sequence":"additional","affiliation":[{"name":"EA7509 IRMAIC, University of Reims-Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank D.","family":"Antonicelli","sequence":"additional","affiliation":[{"name":"EA7509 IRMAIC, University of Reims-Champagne-Ardenne, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelie","family":"Servettaz","sequence":"additional","affiliation":[{"name":"EA7509 IRMAIC, University of Reims-Champagne-Ardenne, 51100 Reims, France"},{"name":"Department of Internal Medicine, Infectious Diseases, and Clinical Immunology, Reims Teaching Hospitals, Robert Debr\u00e9 Hospital, 51100 Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2737","DOI":"10.1002\/art.38098","article-title":"2013 classification criteria for systemic sclerosis: An American College of Rheumatology\/European League against Rheumatism collaborative initiative","volume":"65","author":"Khanna","year":"2013","journal-title":"Arthr. 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