{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:01:38Z","timestamp":1790996498566,"version":"4.1.0"},"reference-count":33,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T00:00:00Z","timestamp":1594598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["JP18bm0204002, JP17bk0104002"],"award-info":[{"award-number":["JP18bm0204002, JP17bk0104002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"name":"KAKENHI","award":["25293357, 18H02959, 16K21633"],"award-info":[{"award-number":["25293357, 18H02959, 16K21633"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Immune attacks are key issues for cell transplantation. To assess the safety and the immune reactions after iPS cells-derived retinal pigment epithelium (iPS-RPE) transplantation, we transplanted HLA homozygote iPS-RPE cells established at an iPS bank in HLA-matched patients with exudative age-related macular degeneration. In addition, local steroids without immunosuppressive medications were administered. We monitored immune rejections by routine ocular examinations as well as by lymphocytes-graft cells immune reaction (LGIR) tests using graft RPE and the patient\u2019s blood cells. In all five of the cases that underwent iPS-RPE transplantation, the presence of graft cells was indicated by clumps or an area of increased pigmentation at 6 months, which became stable with no further abnormal growth in the graft during the 1-year observation period. Adverse events observed included corneal erosion, epiretinal membrane, retinal edema due to epiretinal membrane, elevated intraocular pressure, endophthalmitis, and mild immune rejection in the eye. In the one case exhibiting positive LGIR tests along with a slight fluid recurrence, we administrated local steroid therapy that subsequently resolved the suspected immune attacks. Although the cell delivery strategy must be further optimized, the present results suggest that it is possible to achieve stable survival and safety of iPS-RPE cell transplantation for a year.<\/jats:p>","DOI":"10.3390\/jcm9072217","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T11:03:23Z","timestamp":1594724603000},"page":"2217","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":168,"title":["HLA-Matched Allogeneic iPS Cells-Derived RPE Transplantation for Macular Degeneration"],"prefix":"10.3390","volume":"9","author":[{"given":"Sunao","family":"Sugita","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"},{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michiko","family":"Mandai","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"},{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiko","family":"Hirami","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Takagi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, School of Medicine, Toho University, Tokyo 153-8515, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadao","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Fujihara","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4618-3696","authenticated-orcid":false,"given":"Mitsuhiro","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Midori","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Iseki","sequence":"additional","affiliation":[{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayumi","family":"Hono","sequence":"additional","affiliation":[{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoko","family":"Fujino","sequence":"additional","affiliation":[{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoshi","family":"Koide","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"},{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Sakai","sequence":"additional","affiliation":[{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumiko","family":"Shibata","sequence":"additional","affiliation":[{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoki","family":"Terada","sequence":"additional","affiliation":[{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiro","family":"Nishida","sequence":"additional","affiliation":[{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Dohi","sequence":"additional","affiliation":[{"name":"Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8567, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1277-9122","authenticated-orcid":false,"given":"Masaki","family":"Nomura","sequence":"additional","affiliation":[{"name":"Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8567, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Amano","sequence":"additional","affiliation":[{"name":"Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8567, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Sakaguchi","sequence":"additional","affiliation":[{"name":"Department of Advanced Device Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chikako","family":"Hara","sequence":"additional","affiliation":[{"name":"Department of Advanced Device Medicine, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6283-5466","authenticated-orcid":false,"given":"Kazuichi","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Department of Innovative Visual Science, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Daimon","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Hyogo College of Medicine, Nishinomiya 663-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Igeta","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Hyogo College of Medicine, Nishinomiya 663-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Oda","sequence":"additional","affiliation":[{"name":"Center for Clinical Research and Innovation, Kobe City Medical Center General Hospital, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Utako","family":"Shirono","sequence":"additional","affiliation":[{"name":"Center for Clinical Research and Innovation, Kobe City Medical Center General Hospital, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misato","family":"Tozaki","sequence":"additional","affiliation":[{"name":"Center for Clinical Research and Innovation, Kobe City Medical Center General Hospital, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kota","family":"Totani","sequence":"additional","affiliation":[{"name":"Tomey Corporation, Nagoya 451-0051, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Tomey Corporation, Nagoya 451-0051, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohji","family":"Nishida","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Kurimoto","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayo","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Kobe City Eye Hospital, Kobe 650-0047, Japan"},{"name":"Department of Ophthalmology, Kobe City Medical Center General Hospital Kobe 650-0047, Japan"},{"name":"Laboratory for Retinal Regeneration, RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.stemcr.2016.08.010","article-title":"Successful Transplantation of Retinal Pigment Epithelial Cells from MHC Homozygote iPSCs in MHC-Matched Models","volume":"7","author":"Sugita","year":"2016","journal-title":"Stem Cell Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/j.stemcr.2016.08.011","article-title":"Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors","volume":"7","author":"Sugita","year":"2016","journal-title":"Stem Cell Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1016\/j.stemcr.2017.10.003","article-title":"Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models","volume":"9","author":"Sugita","year":"2017","journal-title":"Stem Cell Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1056\/NEJM200002173420707","article-title":"Age-related macular degeneration","volume":"342","author":"Fine","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/S0161-6420(92)31871-8","article-title":"Prevalence of age-related maculopathy. The Beaver Dam Eye Study","volume":"99","author":"Klein","year":"1992","journal-title":"Ophthalmology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/S0161-6420(95)31034-2","article-title":"The prevalence of age-related maculopathy in the Rotterdam Study","volume":"102","author":"Vingerling","year":"1995","journal-title":"Ophthalmology"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1450","DOI":"10.1016\/S0161-6420(95)30846-9","article-title":"Prevalence of age-related maculopathy in Australia. The Blue Mountains Eye Study","volume":"102","author":"Mitchell","year":"1995","journal-title":"Ophthalmology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2292","DOI":"10.1016\/j.ophtha.2013.03.046","article-title":"Seven-year outcomes in ranibizumab-treated patients in ANCHOR, MARINA, and HORIZON: A multicenter cohort study (SEVEN-UP)","volume":"120","author":"Rofagha","year":"2013","journal-title":"Ophthalmology"},{"key":"ref_9","first-page":"102","article-title":"A technique for retinal pigment epithelium transplantation for age-related macular degeneration secondary to extensive subfoveal scarring","volume":"22","author":"Peyman","year":"1991","journal-title":"Ophthalmic Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1006\/exer.1994.1069","article-title":"Immunologic consequences of intraocular implantation of retinal pigment epithelial allografts","volume":"58","author":"Jiang","year":"1994","journal-title":"Exp. Eye Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1600-0420.1997.tb00239.x","article-title":"Clinical possibilities in retinal pigment epithelial transplantations","volume":"75","author":"Algvere","year":"1997","journal-title":"Acta Ophthalmol. Scand."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1177\/112067219900900310","article-title":"Long-term outcome of RPE allografts in non-immunosuppressed patients with AMD","volume":"9","author":"Algvere","year":"1999","journal-title":"Eur. J. Ophthalmol."},{"key":"ref_13","first-page":"540","article-title":"Allogenic fetal retinal pigment epithelial cell transplant in a patient with geographic atrophy","volume":"19","author":"Weisz","year":"1999","journal-title":"Retina"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1167\/iovs.14-15619","article-title":"Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells","volume":"56","author":"Sugita","year":"2015","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1167\/iovs.17-22703","article-title":"Natural Killer Cell Inhibition by HLA-E Molecules on Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells","volume":"59","author":"Sugita","year":"2018","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1038\/nri1224","article-title":"Ocular immune privilege: Therapeutic opportunities from an experiment of nature","volume":"3","author":"Streilein","year":"2003","journal-title":"Nat. Rev. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1615\/CritRevImmunol.v22.i1.20","article-title":"Immune privilege in the anterior chamber of the eye","volume":"22","author":"Niederkorn","year":"2002","journal-title":"Crit. Rev. Immunol."},{"key":"ref_18","first-page":"381","article-title":"Patch transplants of human fetal retinal pigment epithelium in rabbit and monkey retina","volume":"36","author":"Sheng","year":"1995","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1136\/bjo.88.1.110","article-title":"Autologous peripheral retinal pigment epithelium translocation in patients with subfoveal neovascular membranes","volume":"88","author":"Hofland","year":"2004","journal-title":"Br. J. Ophthalmol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1056\/NEJMoa1608368","article-title":"Autologous Induced Stem-Cell-Derived Retinal Cells for Macular Degeneration","volume":"376","author":"Mandai","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1038\/nmeth.1591","article-title":"A more efficient method to generate integration-free human iPS cells","volume":"8","author":"Okita","year":"2011","journal-title":"Nat. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1007\/s10384-018-0611-8","article-title":"Frequencies of human leukocyte antigen alleles and haplotypes among Japanese patients with age-related macular degeneration","volume":"62","author":"Takagi","year":"2018","journal-title":"Jpn. J. Ophthalmol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3594","DOI":"10.1038\/srep03594","article-title":"A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells","volume":"4","author":"Nakagawa","year":"2014","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.stemcr.2013.12.007","article-title":"Characterization of human induced pluripotent stem cell-derived retinal pigment epithelium cell sheets aiming for clinical application","volume":"2","author":"Kamao","year":"2014","journal-title":"Stem Cell Rep."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1038\/s41421-018-0053-y","article-title":"Human embryonic stem cell-derived retinal pigment epithelium transplants as a potential treatment for wet age-related macular degeneration","volume":"4","author":"Liu","year":"2018","journal-title":"Cell Discov."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1038\/nbt.4114","article-title":"Phase 1 clinical study of an embryonic stem cell-derived retinal pigment epithelium patch in age-related macular degeneration","volume":"36","author":"Fynes","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1016\/j.ophtha.2018.04.037","article-title":"Transplantation of Human Embryonic Stem Cell-Derived Retinal Pigment Epithelial Cells in Macular Degeneration","volume":"125","author":"Mehat","year":"2018","journal-title":"Ophthalmology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/S0140-6736(12)60028-2","article-title":"Embryonic stem cell trials for macular degeneration: A preliminary report","volume":"379","author":"Schwartz","year":"2012","journal-title":"Lancet"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0140-6736(14)61376-3","article-title":"Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt\u2019s macular dystrophy: Follow-up of two open-label phase 1\/2 studies","volume":"385","author":"Schwartz","year":"2015","journal-title":"Lancet"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1126\/scitranslmed.aao4097","article-title":"A bioengineered retinal pigment epithelial monolayer for advanced, dry age-related macular degeneration","volume":"10","author":"Kashani","year":"2018","journal-title":"Sci. Transl. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1002\/jnr.490330205","article-title":"Growth control of cultured microglia","volume":"33","author":"Ganter","year":"1992","journal-title":"J. Neurosci. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1038\/nature10135","article-title":"Immunogenicity of induced pluripotent stem cells","volume":"474","author":"Zhao","year":"2011","journal-title":"Nature"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.stemcr.2017.07.020","article-title":"NK Cell Alloreactivity against KIR-Ligand-Mismatched HLA-Haploidentical Tissue Derived from HLA Haplotype-Homozygous iPSCs","volume":"9","author":"Ichise","year":"2017","journal-title":"Stem Cell Rep."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/9\/7\/2217\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:51:00Z","timestamp":1760176260000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/9\/7\/2217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,13]]},"references-count":33,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2020,7]]}},"alternative-id":["jcm9072217"],"URL":"https:\/\/doi.org\/10.3390\/jcm9072217","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,13]]}}}