{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T20:50:17Z","timestamp":1783198217721,"version":"3.54.6"},"reference-count":28,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation","award":["# 20-14-00074"],"award-info":[{"award-number":["# 20-14-00074"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JDB"],"abstract":"<jats:p>The Notch pathway is an important and evolutionarily conserved signaling system involved in the development of multicellular organisms. Notch signaling plays an important role in the regulation of proliferation and differentiation of many cell types. In this study, we report new aspects of Notch gene participation in oogenesis using our previously generated mutations. The mutations consist of an insertion of an auxiliary element of a transgene construct into the first intron of the gene and a series of directed deletions within the 5\u2032 regulatory region of Notch. We showed that some of these mutations affect Drosophila oogenesis. This insertion, either alone or in combination with the deletion of an insulator sequence, led to lower expression of Notch in the ovaries. As a result, the formation of egg chambers was disturbed in middle oogenesis. These abnormalities have not been described previously and imply one more function of Notch in oogenesis. It can be assumed that Notch is associated with not only follicular epithelium morphogenesis but also cellular mechanisms of oocyte growth.<\/jats:p>","DOI":"10.3390\/jdb10030032","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T02:42:53Z","timestamp":1660099373000},"page":"32","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["New Mutations in the 5\u2032 Region of the Notch Gene Affect Drosophila melanogaster Oogenesis"],"prefix":"10.3390","volume":"10","author":[{"given":"Elena I.","family":"Volkova","sequence":"first","affiliation":[{"name":"Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalya V.","family":"Dorogova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (SB RAS), 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleg V.","family":"Andreyenkov","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saveliy A.","family":"Tikhomirov","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey A.","family":"Demakov","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.gde.2009.04.005","article-title":"Endocytic regulation of Notch signaling","volume":"19","author":"Fortini","year":"2009","journal-title":"Curr. 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