{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T08:26:49Z","timestamp":1793176009251,"version":"4.5.0"},"reference-count":68,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T00:00:00Z","timestamp":1661817600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gatton Academy of Mathematics and Science in Kentucky","award":["None"],"award-info":[{"award-number":["None"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JDB"],"abstract":"<jats:p>Reliable animal model systems are an integral part of biological research. Ever since Thomas Hunt Morgan won a Nobel Prize for genetic work done using the fruit fly (Drosophila melanogaster) as a model organism, it has played a larger and more important role in genetic research. Drosophila models have long been used to study neurodegenerative diseases and have aided in identifying key disease progression biological pathways. Due to the availability of a vast array of genetic manipulation tools, its relatively short lifespan, and its ability to produce many progenies, D. melanogaster has provided the ability to conduct large-scale genetic screens to elucidate possible genetic and molecular interactions in neurodegenerative diseases such as Alzheimer\u2019s disease, Parkinson\u2019s disease, Huntington\u2019s Disease, and Amyotrophic Lateral Sclerosis (ALS). With regards to ALS, many of the gene mutations that have been discovered to be linked to the disease have been modeled in Drosophila to provide a look into a detailed model of pathogenesis. The aim of this review is to summarize key and newer developments in ALS research that have utilized Drosophila and to provide insight into the profound use of Drosophila as a tool for modeling this disease.<\/jats:p>","DOI":"10.3390\/jdb10030036","type":"journal-article","created":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:13:56Z","timestamp":1661904836000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Drosophila melanogaster as a Tool for Amyotrophic Lateral Sclerosis Research"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3349-5738","authenticated-orcid":false,"given":"Krupa N.","family":"Hegde","sequence":"first","affiliation":[{"name":"Gatton Academy of Mathematics and Sciences at Western Kentucky University, Bowling Green, KY 42101, USA"},{"name":"Department of Cell Biology, Yale College, New Haven, CT 06520, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5503-350X","authenticated-orcid":false,"given":"Ajay","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Biology, Western Kentucky University, Bowling Green, KY 42101, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/978-1-4939-6371-3_3","article-title":"The Q-system: A versatile expression system for Drosophila","volume":"1478","author":"Riabinina","year":"2016","journal-title":"Methods Mol. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/gene.10150","article-title":"GAL4 system in Drosophila: A fly geneticist\u2019s Swiss army knife","volume":"34","author":"Duffy","year":"2002","journal-title":"Genesis"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/nmeth.1662","article-title":"MiMIC: A highly versatile transposon insertion resource for engineering Drosophila melanogaster genes","volume":"8","author":"Venken","year":"2011","journal-title":"Nat. Methods"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"31.32.1","DOI":"10.1002\/0471142727.mb3102s111","article-title":"CRISPR-Cas9 genome editing in Drosophila","volume":"111","author":"Gratz","year":"2015","journal-title":"Curr. Protoc. Mol. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1101\/gr.169101","article-title":"A systematic analysis of human disease-associated gene sequences in Drosophila melanogaster","volume":"11","author":"Reiter","year":"2001","journal-title":"Genome Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1146\/annurev.pathol.3.121806.151529","article-title":"Drosophila models of neurodegenerative diseases","volume":"4","author":"Lu","year":"2009","journal-title":"Annu. Rev. Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"91","DOI":"10.31887\/DCNS.2016.18.1\/pnopoulos","article-title":"Huntington disease: A single-gene degenerative disorder of the striatum","volume":"18","author":"Nopoulos","year":"2016","journal-title":"Dialogues Clin. Neurosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"229","DOI":"10.4161\/fly.26279","article-title":"Modeling Huntington disease in Drosophila: Insights into axonal transport defects and modifiers of toxicity","volume":"7","author":"Krench","year":"2013","journal-title":"Fly"},{"key":"ref_9","first-page":"504","article-title":"Parkinson\u2019s disease and its management: Part 1: Disease entity, risk factors, patho-physiology, clinical presentation, and diagnosis","volume":"40","author":"DeMaagd","year":"2015","journal-title":"Pharm. Ther."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"228","DOI":"10.3389\/fneur.2018.00228","article-title":"Modeling Parkinson\u2019s disease in Drosophila: What have we learned for dominant traits?","volume":"9","author":"Xiong","year":"2018","journal-title":"Front. Neurol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Arsac, J.-N., Sedru, M., Dartiguelongue, M., Vulin, J., Davoust, N., Baron, T., and Mollereau, B. (2021). Chronic exposure to paraquat induces alpha-synuclein pathogenic modifications in Drosophila. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222111613"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"91","DOI":"10.31887\/DCNS.2000.2.2\/asschachter","article-title":"Alzheimer\u2019s disease","volume":"2","author":"Schachter","year":"2000","journal-title":"Dialogues Clin. Neurosci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.tibs.2009.11.004","article-title":"Alzheimer\u2019s disease: Insights from Drosophila melanogaster models","volume":"35","author":"Moloney","year":"2010","journal-title":"Trends Biochem. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Jeon, Y., Lee, J.H., Choi, B., Won, S.Y., and Cho, K.S. (2020). Genetic dissection of Alzheimer\u2019s disease using Drosophila models. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21030884"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"171","DOI":"10.4103\/2152-7806.169561","article-title":"A comprehensive review of amyotrophic lateral sclerosis","volume":"6","author":"Zarei","year":"2015","journal-title":"Surg. Neurol. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3109\/17482960802566824","article-title":"Prognostic factors in ALS: A critical review","volume":"10","author":"Logroscino","year":"2009","journal-title":"Amyotroph. Lateral Scler."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1038\/nature10353","article-title":"Mutations in UBQLN2 cause dominant X-linked juvenile and adult-onset ALS and ALS\/dementia","volume":"477","author":"Deng","year":"2011","journal-title":"Nature"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1016\/j.neuron.2018.02.027","article-title":"Genome-wide analyses identify KIF5A as a novel ALS gene","volume":"97","author":"Nicolas","year":"2018","journal-title":"Neuron"},{"key":"ref_19","first-page":"252","article-title":"Current knowledge and recent insights into the genetic basis of amyotrophic lateral sclerosis","volume":"30","author":"Volk","year":"2018","journal-title":"Med. Genet."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1007\/s10072-020-05037-6","article-title":"NEK1 mutations and the risk of amyotrophic lateral sclerosis (ALS): A meta-analysis","volume":"42","author":"Yao","year":"2021","journal-title":"Neurol. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1016\/j.neurobiolaging.2013.03.024","article-title":"Expression of zinc-deficient human superoxide dismutase in Drosophila neurons produces a locomotor defect linked to mitochondrial dysfunction","volume":"34","author":"Bahadorani","year":"2013","journal-title":"Neurobiol. Aging"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1080\/15548627.2019.1580103","article-title":"VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway","volume":"15","author":"Mao","year":"2019","journal-title":"Autophagy"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1007\/s12035-007-0034-x","article-title":"Alsin and the molecular pathways of amyotrophic lateral sclerosis","volume":"36","author":"Chandran","year":"2007","journal-title":"Mol. Neurobiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.devcel.2011.12.009","article-title":"Secreted VAPB\/ALS8 major sperm protein domains modulate mitochondrial localization and morphology via growth cone guidance receptors","volume":"22","author":"Han","year":"2012","journal-title":"Dev. Cell"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wang, P., Deng, J., Dong, J., Liu, J., Bigio, E.H., Mesulam, M., Wang, T., Sun, L., Wang, L., and Lee, A.Y. (2019). TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response. PLoS Genet., 15.","DOI":"10.1371\/journal.pgen.1007947"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"11068","DOI":"10.1074\/jbc.C109.078527","article-title":"Ubiquilin modifies TDP-43 toxicity in a Drosophila model of amyo-trophic lateral sclerosis (ALS)","volume":"285","author":"Hanson","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_27","first-page":"4103","article-title":"FUS-induced neurotoxicity in Drosophila is prevented by downregulating nucleocytoplasmic transport proteins","volume":"27","author":"Steyaert","year":"2018","journal-title":"Hum. Mol. Genet."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Deng, J., Yang, M., Chen, Y., Chen, X., Liu, J., Sun, S., Cheng, H., Li, Y., Bigio, E.H., and Mesulam, M. (2015). FUS interacts with HSP60 to promote mitochondrial damage. PLoS Genet, 11.","DOI":"10.1371\/journal.pgen.1005357"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1126\/science.1256800","article-title":"C9orf72 repeat expansions cause neurodegeneration in Drosophila through arginine-rich proteins","volume":"345","author":"Mizielinska","year":"2014","journal-title":"Science"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/nature14973","article-title":"The C9orf72 repeat expansion disrupts nucleocytoplasmic transport","volume":"525","author":"Zhang","year":"2015","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1038\/364362c0","article-title":"Mutations in Cu\/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis","volume":"364","author":"Rosen","year":"1993","journal-title":"Nature"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/nn.3584","article-title":"State of play in amyotrophic lateral sclerosis genetics","volume":"17","author":"Renton","year":"2014","journal-title":"Nat. Neurosci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"8574","DOI":"10.1073\/pnas.92.19.8574","article-title":"Subunit-destabilizing mutations in Drosophila copper\/zinc superoxide dismutase: Neuropathology and a model of dimer dysequilibrium","volume":"92","author":"Phillips","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1038\/534","article-title":"Extension of Drosophila lifespan by overexpression of human SOD1 in motorneurons","volume":"19","author":"Parkes","year":"1998","journal-title":"Nat. Genet."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2308","DOI":"10.1016\/j.febslet.2009.06.023","article-title":"Manipulation of Sod1 expression ubiquitously, but not in the nervous system or muscle, impacts age-related parameters in Drosophila","volume":"583","author":"Martin","year":"2009","journal-title":"FEBS Lett."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"24972","DOI":"10.1074\/jbc.M804817200","article-title":"A Drosophila model for amyotrophic lateral sclerosis reveals motor neuron damage by human SOD1","volume":"283","author":"Watson","year":"2008","journal-title":"J. Biol. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1534\/genetics.116.190850","article-title":"Human SOD1 ALS mutations in a Drosophila knock-in model cause severe phenotypes and reveal dosage-sensitive gain- and loss-of-function components","volume":"205","author":"Held","year":"2017","journal-title":"Genetics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2347","DOI":"10.1523\/JNEUROSCI.1771-18.2019","article-title":"Circuit dysfunction in SOD1-ALS model first detected in sensory feedback prior to motor neuron degeneration is alleviated by BMP signaling","volume":"39","author":"Held","year":"2019","journal-title":"J. Neurosci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1038\/ng1001-166","article-title":"A gene encoding a putative GTPase regulator is mutated in familial amyotrophic lateral sclerosis 2","volume":"29","author":"Hadano","year":"2001","journal-title":"Nat. Genet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1093\/hmg\/ddg184","article-title":"ALS2, a novel guanine nucleotide exchange factor for the small GTPase Rab5, is implicated in endosomal dynamics","volume":"12","author":"Otomo","year":"2003","journal-title":"Hum. Mol. Genet."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1111\/gtc.12146","article-title":"Age-dependent deterioration of locomotion in Drosophila melanogaster deficient in the homologue of amyotrophic lateral sclerosis 2","volume":"19","author":"Takayama","year":"2014","journal-title":"Genes Cells"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.brainres.2014.09.064","article-title":"A fruitful endeavor: Modeling ALS in the fruit fly","volume":"1607","author":"Casci","year":"2015","journal-title":"Brain Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1086\/425287","article-title":"A mutation in the vesicle-trafficking protein VAPB causes late-onset spinal muscular atrophy and amyotrophic lateral sclerosis","volume":"75","author":"Nishimura","year":"2004","journal-title":"Am. J. Hum. Genet."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1093\/brain\/awm190","article-title":"Oxidative and endoplasmic reticulum stress interplay in sporadic amyotrophic lateral sclerosis","volume":"130","author":"Ilieva","year":"2007","journal-title":"Brain"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1093\/hmg\/ddm303","article-title":"hVAPB, the causative gene of a heterogeneous group of motor neuron diseases in humans, is functionally interchangeable with its Drosophila homologue DVAP-33A at the neuromuscular junction","volume":"17","author":"Chai","year":"2008","journal-title":"Hum. Mol. Genet."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ratnaparkhi, A., Lawless, G.M., Schweizer, F.E., Golshani, P., and Jackson, G.R. (2008). A Drosophila model of ALS: Human ALS-associated mutation in VAP33A suggests a dominant negative mechanism. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0002334"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3389\/fnmol.2019.00025","article-title":"Molecular mechanisms of TDP-43 misfolding and pathology in amyotrophic lateral sclerosis","volume":"12","author":"Prasad","year":"2019","journal-title":"Front. Mol. Neurosci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1073\/pnas.0913602107","article-title":"A Drosophila model for TDP-43 proteinopathy","volume":"107","author":"Li","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"7729","DOI":"10.1523\/JNEUROSCI.5894-09.2010","article-title":"TDP-43 mediates degeneration in a novel Drosophila model of disease caused by mutations in VCP\/p97","volume":"30","author":"Ritson","year":"2010","journal-title":"J. Neurosci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Zhan, L., Hanson, K.A., Kim, S.H., Tare, A., and Tibbetts, R.S. (2013). Identification of genetic modifiers of TDP-43 neurotoxicity in Drosophila. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057214"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1126\/science.1166066","article-title":"Mutations in the FUS\/TLS gene on chromosome 16 cause familial amyotrophic lateral sclerosis","volume":"323","author":"Kwiatkowski","year":"2009","journal-title":"Science"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1208","DOI":"10.1126\/science.1165942","article-title":"Mutations in FUS, an RNA processing protein, cause familial amyotrophic lateral sclerosis type 6","volume":"323","author":"Vance","year":"2009","journal-title":"Science"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1007\/s13238-011-1065-7","article-title":"Expression of human FUS protein in Drosophila leads to progressive neurodegeneration","volume":"2","author":"Chen","year":"2011","journal-title":"Protein Cell"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2510","DOI":"10.1093\/hmg\/ddr150","article-title":"A Drosophila model of FUS-related neurodegeneration reveals genetic interaction between FUS and TDP-43","volume":"20","author":"Lanson","year":"2011","journal-title":"Hum. Mol. Genet."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1750-1326-7-10","article-title":"Motor neuron apoptosis and neuromuscular junction perturbation are prominent features in a Drosophila model of Fus-mediated ALS","volume":"7","author":"Xia","year":"2012","journal-title":"Mol. Neurodegener."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3810","DOI":"10.1093\/hmg\/ddu094","article-title":"The ALS gene FUS regulates synaptic transmission at the Drosophila neuromuscular junction","volume":"23","author":"Machamer","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"5583","DOI":"10.1038\/s41467-019-13383-z","article-title":"Muscleblind acts as a modifier of FUS toxicity by modulating stress granule dynamics and SMN localization","volume":"10","author":"Casci","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1007\/s00401-021-02333-z","article-title":"DDX17 is involved in DNA damage repair and modifies FUS toxicity in an RGG-domain dependent manner","volume":"142","author":"Fortuna","year":"2021","journal-title":"Acta Neuropathol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1038\/s41593-021-00859-9","article-title":"Interactions be-tween ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway","volume":"24","author":"Lin","year":"2021","journal-title":"Nat. Neurosci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"104837","DOI":"10.1016\/j.nbd.2020.104837","article-title":"Inactivation of hippo and cJun-N-terminal Kinase (JNK) signaling mitigate FUS mediated neurodegeneration in vivo","volume":"140","author":"Gogia","year":"2020","journal-title":"Neurobiol. Dis."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Scaramuzzino, C., Monaghan, J., Milioto, C., Lanson, N.A., Maltare, A., Aggarwal, T., Casci, I., Fackelmayer, F.O., Pennuto, M., and Pandey, U.B. (2013). Protein arginine methyltransferase 1 and 8 interact with FUS to modify its sub-cellular distribution and toxicity in vitro and in vivo. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0061576"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"7778","DOI":"10.1073\/pnas.1219643110","article-title":"Expanded GGGGCC repeat RNA associated with amyotrophic lateral sclerosis and frontotemporal dementia causes neurodegeneration","volume":"110","author":"Xu","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e08881","DOI":"10.7554\/eLife.08881","article-title":"GGGGCC microsatellite RNA is neuritically localized, induces branching defects, and perturbs transport granule function","volume":"4","author":"Burguete","year":"2015","journal-title":"Elife"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/s40478-018-0555-8","article-title":"Dipeptide repeat proteins activate a heat shock response found in C9ORF72-ALS\/FTLD patients","volume":"6","author":"Mordes","year":"2018","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1038\/nature14974","article-title":"GGGGCC repeat expansion in C9orf72 compromises nucleocytoplasmic transport","volume":"525","author":"Freibaum","year":"2015","journal-title":"Nature"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1750-1326-8-35","article-title":"Drosophila melanogaster as a model organism for Alzheimer\u2019s disease","volume":"8","author":"Voigt","year":"2013","journal-title":"Mol. Neurodegener."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"407","DOI":"10.3390\/ijms10020407","article-title":"Drosophila melanogaster as a model organism of brain diseases","volume":"10","author":"Jeibmann","year":"2009","journal-title":"Int. J. Mol. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"279","DOI":"10.3389\/fneur.2020.00279","article-title":"The peripheral immune system and amyotrophic lateral sclerosis","volume":"11","author":"McCombe","year":"2020","journal-title":"Front. Neurol."}],"container-title":["Journal of Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2221-3759\/10\/3\/36\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:20:14Z","timestamp":1760142014000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2221-3759\/10\/3\/36"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,30]]},"references-count":68,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["jdb10030036"],"URL":"https:\/\/doi.org\/10.3390\/jdb10030036","relation":{},"ISSN":["2221-3759"],"issn-type":[{"value":"2221-3759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,30]]}}}