{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:04:01Z","timestamp":1792872241529,"version":"4.5.0"},"reference-count":61,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JDB"],"abstract":"<jats:p>The proportion of spermatozoa with progressive motility is widely used to evaluate the quality of a single ejaculate. However, the cellular and physiological mechanisms underlying this trait remain unclear. The present study examined the association between the progressive motility of bovine spermatozoa, their quality and their fertilization competence in vitro, and subsequently the association with the developmental morphokinetics of the formed embryos. Fresh ejaculates were classified and divided into groups with high (HPM), medium (MPM), or low (LPM) progressive motility. Then, spermatozoa were evaluated for their morphology, plasma membrane integrity, mitochondrial membrane potential, oxidative status, and acrosome integrity. The findings revealed that spermatozoa from HPM ejaculates enhanced motility in association with higher mitochondrial membrane potential relative to the LPM group, suggesting higher metabolic potential. No differences were recorded in fertilization competence among groups; however, the developmental kinetics of the formed embryos, determined by a time-lapse system, differed; embryos derived from HPM spermatozoa cleaved earlier to the two-, three-, and four-cell stages than embryos derived from MPM spermatozoa, suggesting that HPM-derived embryos are of good quality. Our findings suggest that progressive motility is not only a motility characteristic; it also reflects cellular quality of spermatozoa and the formed embryo.<\/jats:p>","DOI":"10.3390\/jdb14020024","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T12:30:51Z","timestamp":1779280251000},"page":"24","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Association Between the Progressive Motility of Bovine Spermatozoa and the Developmental Morphokinetics of In Vitro-Derived Embryos"],"prefix":"10.3390","volume":"14","author":[{"given":"Shir","family":"Maizus","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorit","family":"Kalo","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7826-9702","authenticated-orcid":false,"given":"Tanya","family":"Kogan","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 7610001, Israel"},{"name":"Sion Artificial Insemination Center, Gedera 7057102, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel","family":"Michaelov","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3026-4915","authenticated-orcid":false,"given":"Zvi","family":"Roth","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.3168\/jds.S0022-0302(01)70158-0","article-title":"Reproductive loss in high-producing dairy cattle: Where will it end?","volume":"84","author":"Lucy","year":"2001","journal-title":"J. Dairy Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3168\/jdsc.2023-0375","article-title":"JDS Communications special issue: Advances in Dairy Cow Fertility\u2014Introduction","volume":"4","author":"Lucy","year":"2023","journal-title":"JDS Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1186\/2046-0481-57-3-158","article-title":"Temporal trends in reproductive performance in Irish dairy herds and associated risk factors","volume":"57","author":"Mee","year":"2004","journal-title":"Ir. Vet. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"10272","DOI":"10.3168\/jds.2017-13399","article-title":"A 100-Year Review: Historical development of female reproductive physiology in dairy cattle","volume":"100","author":"Thatcher","year":"2017","journal-title":"J. Dairy Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Gatius, F. (2024). Advances in Dairy Cattle Reproduction\u2014A Foreword. Animals, 14.","DOI":"10.3390\/ani14182650"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8670","DOI":"10.3168\/jds.2023-24376","article-title":"Reproductive physiological outcomes of dairy cows with different genomic merit for fertility: Biomarkers, uterine health, endocrine status, estrus features, and response to ovarian synchronization","volume":"107","author":"Sitko","year":"2024","journal-title":"J. Dairy Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/j.1939-4640.1993.tb03247.x","article-title":"In Vitro Evaluation of Sperm Quality: An Opinion","volume":"14","author":"Amann","year":"1993","journal-title":"J. Androl."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1093\/tas\/txz174","article-title":"Selection for bull fertility: A review","volume":"4","author":"Butler","year":"2020","journal-title":"Transl. Anim. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1111\/j.1439-0531.2010.01603.x","article-title":"Objective Sperm Motion Analysis to Assess Dairy Bull Fertility Using Computer-Aided System\u2014A Review","volume":"46","author":"Kathiravan","year":"2011","journal-title":"Reprod. Domest. Anim."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/j.theriogenology.2007.04.005","article-title":"Overview of sexing sperm","volume":"68","author":"Seidel","year":"2007","journal-title":"Theriogenology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/and.12093","article-title":"Computer-aided sperm analysis: Past, present and future","volume":"46","author":"Lu","year":"2014","journal-title":"Andrologia"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kogan, T., Dahan, D.G., Laor, R., Argov-Argaman, N., Zeron, Y., Komsky-Elbaz, A., Kalo, D., and Roth, Z. (2021). Association between Fatty Acid Composition, Cryotolerance and Fertility Competence of Progressively Motile Bovine Spermatozoa. Animals, 11.","DOI":"10.3390\/ani11102948"},{"key":"ref_13","first-page":"e58622","article-title":"Fluorimetric techniques for the assessment of sperm membranes","volume":"141","author":"Roth","year":"2018","journal-title":"JoVE"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/(SICI)1098-2795(199906)53:2<222::AID-MRD11>3.0.CO;2-L","article-title":"Organelle-specific probe JC-1 identifies membrane potential differences in the mitochondrial function of bovine sperm","volume":"53","author":"Garner","year":"1999","journal-title":"Mol. Reprod. Dev. Inc. Gamete Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"11226","DOI":"10.3168\/jds.2021-20319","article-title":"Comprehensive functional analysis reveals that acrosome integrity and viability are key variables distinguishing artificial insemination bulls of varying fertility","volume":"104","author":"Bernecic","year":"2021","journal-title":"J. Dairy Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1262\/jrd.2014-140","article-title":"Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality","volume":"61","author":"Takeda","year":"2015","journal-title":"J. Reprod. Dev."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kalo, D., Reches, D., Netta, N., Komsky-Elbaz, A., Zeron, Y., Moallem, U., and Roth, Z. (2022). Carryover effects of feeding bulls with an omega-3-enriched-diet-From spermatozoa to developed embryos. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0265650"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.theriogenology.2022.10.021","article-title":"Membrane remodulation and hyperactivation are impaired in frozen-thawed sperm of low-fertility bulls","volume":"195","author":"Kenny","year":"2023","journal-title":"Theriogenology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1530\/REP-20-0286","article-title":"Effect of aflatoxin B1 on bovine spermatozoa\u2019s proteome and embryo\u2019s transcriptome","volume":"160","author":"Kalo","year":"2020","journal-title":"Reproduction"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"107630","DOI":"10.1016\/j.anireprosci.2024.107630","article-title":"Transcriptomics of bovine sperm and oocytes","volume":"271","author":"Kasimanickam","year":"2024","journal-title":"Anim. Reprod. Sci."},{"key":"ref_21","first-page":"e240051","article-title":"Seminal proteoforms from bulls with contrasting semen freezability: A story deciphered by top-down mass spectrometry","volume":"169","author":"Gomes","year":"2025","journal-title":"Reproduction"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1530\/RAF-22-0037","article-title":"Extracellular vesicles from seminal plasma to improve fertilizing capacity of bulls","volume":"3","author":"Capra","year":"2022","journal-title":"Reprod. Fertil."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/S0093-691X(97)00310-5","article-title":"Relationship between sperm-zona pellucida binding assays and the 56-day nonreturn rate of cattle inseminated with frozen-thawed bull semen","volume":"48","author":"Fazeli","year":"1997","journal-title":"Theriogenology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1530\/REP-13-0123","article-title":"Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome","volume":"146","author":"Feitosa","year":"2013","journal-title":"Reproduction"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4765","DOI":"10.1038\/s41598-023-31268-6","article-title":"Embryo morphokinetics derived from fresh and vitrified bovine oocytes predict blastocyst development and nuclear abnormalities","volume":"13","author":"Benedetti","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Yaacobi-Artzi, S., Kalo, D., and Roth, Z. (2022). Association between the morphokinetics of in-vitro-derived bovine embryos and the transcriptomic profile of the derived blastocysts. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0276642"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1262\/jrd.10-149N","article-title":"Season-induced changes in bovine sperm motility following a freeze-thaw procedure","volume":"58","author":"Orgal","year":"2012","journal-title":"J. Reprod. Dev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.reprotox.2016.11.001","article-title":"Effect of the herbicide atrazine and its metabolite DACT on bovine sperm quality","volume":"67","author":"Roth","year":"2017","journal-title":"Reprod. Toxicol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1071\/RD10330","article-title":"Involvement of the sphingolipid ceramide in heat-shock-induced apoptosis of bovine oocytes","volume":"23","author":"Kalo","year":"2011","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/j.1939-4640.2002.tb02279.x","article-title":"Bicarbonate stimulation of boar sperm motility via a protein kinase A-dependent pathway: Between-cell and between-ejaculate differences are not due to deficiencies in protein kinase A activation","volume":"23","author":"Holt","year":"2002","journal-title":"J. Androl."},{"key":"ref_31","unstructured":"Barth, A.D., and Oko, R.J. (1989). Abnormal Morphology of Bovine Spermatozoa, Iowa State University Press."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Dementieva, N.V., Dysin, A.P., Shcherbakov, Y.S., Nikitkina, E.V., Musidray, A.A., Petrova, A.V., Mitrofanova, O.V., Plemyashov, K.V., Azovtseva, A.I., and Griffin, D.K. (2024). Risk of sperm disorders and impaired fertility in frozen\u2013thawed bull semen: A genome-wide association study. Animals, 14.","DOI":"10.3390\/ani14020251"},{"key":"ref_33","first-page":"525","article-title":"Bovine sperm abnormalities: Prevalence, etiology and mechanisms leading to infertility","volume":"6","author":"Thundathil","year":"2014","journal-title":"Clin. Theriogenol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1038\/aja.2010.97","article-title":"The epididymis, cytoplasmic droplets and male fertility","volume":"13","author":"Cooper","year":"2010","journal-title":"Asian J. Androl."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.theriogenology.2006.08.002","article-title":"Roles of lipid peroxidation and cytoplasmic droplets on in vitro fertilization capacity of sperm collected from bovine epididymides stored at 4 and 34 C","volume":"67","author":"Nichi","year":"2007","journal-title":"Theriogenology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1071\/RD05120","article-title":"Moving to the beat: A review of mammalian sperm motility regulation","volume":"18","author":"Turner","year":"2005","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1002\/rmb2.12381","article-title":"Saturated fatty acids accelerate linear motility through mitochondrial ATP production in bull sperm","volume":"20","author":"Islam","year":"2021","journal-title":"Reprod. Med. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/biolre\/ioaf081","article-title":"Improved evaluation of mitochondrial membrane potential in bovine spermatozoa using JC-1 with flow cytometry","volume":"113","author":"Zezeski","year":"2025","journal-title":"Biol. Reprod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"R163","DOI":"10.1530\/REP-13-0178","article-title":"Mitochondria functionality and sperm quality","volume":"146","author":"Amaral","year":"2013","journal-title":"Reproduction"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Gualtieri, R., Kalthur, G., Barbato, V., Di Nardo, M., Adiga, S.K., and Talevi, R. (2021). Mitochondrial dysfunction and oxidative stress caused by cryopreservation in reproductive cells. Antioxidants, 10.","DOI":"10.3390\/antiox10030337"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1017\/S0967199414000720","article-title":"Progressive motility\u2014A potential predictive parameter for semen fertilization capacity in bovines","volume":"24","author":"Li","year":"2016","journal-title":"Zygote"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"17931","DOI":"10.1038\/s41598-017-18123-1","article-title":"Single-cell-based evaluation of sperm progressive motility via fluorescent assessment of mitochondria membrane potential","volume":"7","author":"Moscatelli","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.urology.2011.12.042","article-title":"Mitochondrial respiratory efficiency is positively correlated with human sperm motility","volume":"79","author":"Ferramosca","year":"2012","journal-title":"Urology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/978-1-60327-517-0_8","article-title":"Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5\u2032,6,6\u2032-tetrachloro-1,1\u2032,3,3\u2032-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) by using fluorescence-activated flow cytometry","volume":"477","author":"Guthrie","year":"2008","journal-title":"Methods Mol. Biol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s12958-024-01252-4","article-title":"The impact of mitochondrial impairments on sperm function and male fertility: A systematic review","volume":"22","author":"Firouza","year":"2024","journal-title":"Reprod. Biol. Endocrinol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Xu, Z., Yan, Q., Zhang, K., Lei, Y., Zhou, C., Ren, T., Gao, N., Wen, F., and Li, X. (2025). Mitochondrial Regulation of Spermatozoa Function: Metabolism, Oxidative Stress and Therapeutic Insights. Animals, 15.","DOI":"10.3390\/ani15152246"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"703","DOI":"10.21451\/1984-3143-AR2018-0041","article-title":"Impacts of oxidative stress on bovine sperm function and subsequent in vitro embryo development","volume":"15","author":"Bollwein","year":"2018","journal-title":"Anim. Reprod."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/S0093-691X(99)00129-6","article-title":"Reactive oxygen species requirements for bovine sperm capacitation and acrosome reaction","volume":"52","author":"Beorlegui","year":"1999","journal-title":"Theriogenology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.redox.2015.02.004","article-title":"Advancing age increases sperm chromatin damage and impairs fertility in peroxiredoxin 6 null mice","volume":"5","author":"Ozkosem","year":"2015","journal-title":"Redox Biol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1007\/s11626-024-00944-w","article-title":"The protective effects of antioxidants against endogenous and exogenous oxidative stress on bull sperm","volume":"60","author":"Younus","year":"2024","journal-title":"Vitr. Cell. Dev. Biol. Anim."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1093\/humupd\/dmad006","article-title":"Contribution of semen to early embryo development: Fertilization and beyond","volume":"29","author":"Mecca","year":"2023","journal-title":"Hum. Reprod. Update"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"469","DOI":"10.17221\/8437-VETMED","article-title":"Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa","volume":"60","author":"Reckova","year":"2015","journal-title":"Vet. Med. Czech"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Y\u00e1niz, J.L., Palac\u00edn, I., Silvestre, M.A., Hidalgo, C.O., Tamargo, C., and Santolaria, P. (2021). Ability of the ISAS3Fun method to detect sperm acrosome integrity and its potential to discriminate between high and low field fertility bulls. Biology, 10.","DOI":"10.3390\/biology10111135"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s12610-016-0045-4","article-title":"Sperm quality and paternal age: Effect on blastocyst formation and pregnancy rates","volume":"27","author":"Chapuis","year":"2017","journal-title":"Basic Clin. Androl."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.theriogenology.2011.03.007","article-title":"Relationship between sperm motility, morphology and the fertility of stallions","volume":"76","author":"Love","year":"2011","journal-title":"Theriogenology"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Sugimura, S., Akai, T., Hashiyada, Y., Somfai, T., Inaba, Y., Hirayama, M., Yamanouchi, T., Matsuda, H., Kobayashi, S., and Aikawa, Y. (2012). Promising system for selecting healthy in vitro\u2013fertilized embryos in cattle. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0036627"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2652","DOI":"10.1093\/humrep\/16.12.2652","article-title":"Early embryo cleavage is a strong indicator of embryo quality in human IVF","volume":"16","author":"Lundin","year":"2001","journal-title":"Hum. Reprod."},{"key":"ref_58","first-page":"540","article-title":"Early cleavage of human embryos to the two-cell stage. A simple, effective indicator of implantation and pregnancy in intracytoplasmic sperm injection","volume":"47","author":"Isiklar","year":"2002","journal-title":"J. Reprod. Med."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e20200041","DOI":"10.1590\/1984-3143-ar2020-0041","article-title":"Effect of environmental contamination on female and male gametes\u2013A lesson from bovines","volume":"17","author":"Roth","year":"2020","journal-title":"Anim. Reprod."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1111\/andr.12781","article-title":"Impact of sperm characteristics on time-lapse embryo morphokinetic parameters and clinical outcome of conventional in vitro fertilization","volume":"8","author":"Nikolova","year":"2020","journal-title":"Andrology"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e12997","DOI":"10.1111\/and.12997","article-title":"Is there any correlation between sperm parameters and chromatin quality with embryo morphokinetics in patients with male infertility?","volume":"50","author":"Mangoli","year":"2018","journal-title":"Andrologia"}],"container-title":["Journal of Developmental Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2221-3759\/14\/2\/24\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T13:05:38Z","timestamp":1779282338000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2221-3759\/14\/2\/24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":61,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["jdb14020024"],"URL":"https:\/\/doi.org\/10.3390\/jdb14020024","relation":{},"ISSN":["2221-3759"],"issn-type":[{"value":"2221-3759","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]}}}