{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:53:44Z","timestamp":1791917624572,"version":"4.3.4"},"reference-count":50,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T00:00:00Z","timestamp":1597363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["51673029"],"award-info":[{"award-number":["51673029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["61875015"],"award-info":[{"award-number":["61875015"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Talent Fund","award":["2016000012113ZK34"],"award-info":[{"award-number":["2016000012113ZK34"]}]},{"name":"Beijing Municipal Health Commission","award":["PXM2018026275000001"],"award-info":[{"award-number":["PXM2018026275000001"]}]},{"name":"Beijing Municipal Health Commission","award":["BMC2018-4"],"award-info":[{"award-number":["BMC2018-4"]}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/K029592\/1"],"award-info":[{"award-number":["EP\/K029592\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Preventing the development of osteomyelitis while enhancing bone regeneration is challenging, with relatively little progress to date in translating promising technologies to the clinic. Nanoscale hydroxyapatite (nHA) has been employed as a bone graft substitute, and recent work has shown that it may be modified with silver to introduce antimicrobial activity against known pathogens. The aim of this study was to incorporate silver-doped nHA into electrospun scaffolds for applications in bone repair. Silver-doped nHA was produced using a modified, rapid mixing, wet precipitation method at 2, 5, 10 mol.% silver. The silver-doped nHA was added at 20 wt.% to a polycaprolactone solution for electrospinning. Bacteria studies demonstrated reduced bacterial presence, with Escherichia coli and Staphylococcus aureus undetectable after 96 h of exposure. Mesenchymal stem cells (MSCs) were used to study both toxicity and osteogenicity of the scaffolds using PrestoBlue\u00ae and alkaline phosphatase (ALP) assays. Innovative silver nHA scaffolds significantly reduced E. coli and S. aureus bacterial populations while maintaining cytocompatibility with mammalian cells and enhancing the differentiation of MSCs into osteoblasts. It was concluded that silver-doped nHA containing scaffolds have the potential to act as an antimicrobial device while supporting bone tissue healing for applications in orthopedic and dental bone surgery.<\/jats:p>","DOI":"10.3390\/jfb11030058","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T13:00:18Z","timestamp":1597410018000},"page":"58","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Electrospun Scaffolds Containing Silver-Doped Hydroxyapatite with Antimicrobial Properties for Applications in Orthopedic and Dental Bone Surgery"],"prefix":"10.3390","volume":"11","author":[{"given":"Thomas E.","family":"Paterson","sequence":"first","affiliation":[{"name":"School of Clinical Dentistry, University of Sheffield, Shefield 0114, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Shi","sequence":"additional","affiliation":[{"name":"Beijing Laboratory of Biomedical Materials, Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Tian","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, China"},{"name":"Central Laboratory, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline J.","family":"Harrison","sequence":"additional","affiliation":[{"name":"School of Clinical Dentistry, University of Sheffield, Shefield 0114, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mailys","family":"De Sousa Mendes","sequence":"additional","affiliation":[{"name":"Certara, Simcyp division, Sheffield 0114, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5234-1104","authenticated-orcid":false,"given":"Paul V.","family":"Hatton","sequence":"additional","affiliation":[{"name":"School of Clinical Dentistry, University of Sheffield, Shefield 0114, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9952-7296","authenticated-orcid":false,"given":"Zhou","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Micro-nano Energy and Sensor, Beijing institute of Nanoenergy and Nanosystems, Chinese Academy of Sciences, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilida","family":"Ortega","sequence":"additional","affiliation":[{"name":"School of Clinical Dentistry, University of Sheffield, Shefield 0114, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1053\/berh.1999.0004","article-title":"Micro-organisms responsible for osteo-articular infections","volume":"13","author":"Bouza","year":"1999","journal-title":"Best Pract. 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