{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T17:15:23Z","timestamp":1783703723314,"version":"3.55.0"},"reference-count":92,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T00:00:00Z","timestamp":1603411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Adlerbertska Forskningsstiftelsen","award":["09\/2019"],"award-info":[{"award-number":["09\/2019"]}]},{"DOI":"10.13039\/501100008584","name":"Stiftelsen L\u00e4ngmanska Kulturfonden","doi-asserted-by":"publisher","award":["BA18-0708"],"award-info":[{"award-number":["BA18-0708"]}],"id":[{"id":"10.13039\/501100008584","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>An accumulating body of evidence reports the synthesis and biomedical applications of silver nanoparticles. However, the studies regarding the use of maleic acid and citric acid in the synthesis of nano-sized silver particles (AgNPs) and micro-sized silver particles (AgMPs) as well as their antibacterial, antifungal, and anticancer activities have not been reported. In the current study, we synthesized AgNPs and AgMPs using maleic acid and citric acid as capping agents and have characterized them by UV-Vis, energy-dispersive X-Ray spectroscopy (EDS), X-Ray diffraction (XRD), and scanning electron microscope (SEM) analysis. The capped silver particles were examined for their antimicrobial activity and cytotoxicity against bacteria, fungi, and brine shrimp. Additionally, the anticancer activity of these particles was tested against human breast and liver cancer cell lines. The free radical scavenging activity of capped silver particles was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. SEM analysis revealed a round plate-like morphology of maleic acid capped particles with an average size of 39 \u00b1 4 nm, whereas citric acid capped particles display flower-shaped morphology with rough surfaces and an average size of 250 \u00b1 5 nm. The uncapped AgMPs were hexagonal with 500 \u00b1 4 nm size. EDS and XRD analysis confirmed the presence of Ag and face-centered cubic crystalline nature, respectively. Functionally, capped silver particles exhibited antibacterial activity against Gram-positive (Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus) and Gram-negative bacteria (Salmonella setubal, Enterobacter aerogenes, and Agrobacterium tumefaciens). The bactericidal activity was more active against Gram-negative bacteria with minimum inhibitory concentration (MIC) as low as 5 ppm as compared to 25 ppm for Gram-positive. Similarly, the silver particles demonstrated antifungal activity by inhibiting the growth of five fungal strains (Mucor species, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, and Fusarium solani) up to 50% at the concentration of 500 ppm. Additionally, these particles showed substantial toxicity against brine shrimp and also significantly inhibited the proliferation of breast cancer (MCF7) and liver cancer (HePG2) cell lines (IC50 8.9\u201318.56 \u00b5M). Uncapped AgMPs were less effective, inhibiting only the proliferation of MCF7 cells with IC50 46.54 \u00b5M. Besides cytotoxicity, these particles acted as potential antioxidants, showing free radical scavenging up to 74.4% in a concentration-dependent manner. Taken together, our results showed that the modifiers affect the shape and size of silver particles and may, in part, contribute to the antimicrobial and antioxidant activity of silver particles. However, the contribution of maleic acid and citric acid in enhancing the antimicrobial, anticancer, and antioxidant potential independent of silver nano and microparticles needs to be studied further. In vivo experiments may determine the therapeutic effectiveness of silver particles capped with these modifiers.<\/jats:p>","DOI":"10.3390\/jfb11040076","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T08:59:28Z","timestamp":1603443568000},"page":"76","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Synthesis of Functional Silver Nanoparticles and Microparticles with Modifiers and Evaluation of Their Antimicrobial, Anticancer, and Antioxidant Activity"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0854-7428","authenticated-orcid":false,"given":"Erum","family":"Dilshad","sequence":"first","affiliation":[{"name":"Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology (CUST), Islamabad 44000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmoona","family":"Bibi","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Biosciences, Faculty of Health and Life Sciences, Capital University of Science and Technology (CUST), Islamabad 44000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2405-1358","authenticated-orcid":false,"given":"Nadeem Ahmed","family":"Sheikh","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, Faculty of Engineering and Technology, International Islamic University, Islamabad 44000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2180-9610","authenticated-orcid":false,"given":"Khairul Fikri","family":"Tamrin","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Manufacturing Engineering, Faculty of Engineering, University Malaysia Sarawak (UNIMAS), Kota Samarahan 94300, Sarawak, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qaisar","family":"Mansoor","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Genetic Engineering (IBGE), Islamabad 44000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5882-2117","authenticated-orcid":false,"given":"Qaisar","family":"Maqbool","sequence":"additional","affiliation":[{"name":"National Institute of Vacuum Science and Technology (NINVAST), Islamabad 44000, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0792-8296","authenticated-orcid":false,"given":"Muhammad","family":"Nawaz","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 413 46 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2350","DOI":"10.1002\/anie.200200562","article-title":"Higher-order organization by mesoscale self-assembly and transformation of hybrid nanostructures","volume":"42","author":"Mann","year":"2003","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s12598-010-0139-7","article-title":"Preparation of microsized silver crystals with different morphologies by a wet-chemical method","volume":"29","author":"Cai","year":"2010","journal-title":"Rare Met."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1002\/adma.200390087","article-title":"One-dimensional nanostructures: Synthesis, characterization, and applications","volume":"15","author":"Xia","year":"2003","journal-title":"Adv. Mater."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2969","DOI":"10.1021\/cm0527773","article-title":"Surface functionalized silver nanoparticles for ultrahigh conductive polymer composites","volume":"18","author":"Jiang","year":"2006","journal-title":"Chem. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8333","DOI":"10.1021\/jp001803b","article-title":"Structure and stability of silver nanoparticles in aqueous solution produced by laser ablation","volume":"104","author":"Kohno","year":"2000","journal-title":"J. Phys. Chem. B"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1021\/ar7002804","article-title":"Noble metals on the nanoscale: Optical and photothermal properties and some applications in imaging, sensing, biology, and medicine","volume":"41","author":"Jain","year":"2008","journal-title":"Acc. Chem. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"16248","DOI":"10.1021\/jp063826h","article-title":"Silver colloid nanoparticles: Synthesis, characterization, and their antibacterial activity","volume":"110","author":"Kvitek","year":"2006","journal-title":"J. Phys. Chem. B"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.nano.2015.11.016","article-title":"Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity","volume":"12","author":"Duran","year":"2016","journal-title":"Nanomedicine"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.actbio.2007.11.006","article-title":"Strain specificity in antimicrobial activity of silver and copper nanoparticles","volume":"4","author":"Ruparelia","year":"2008","journal-title":"Acta Biomater."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ijfoodmicro.2018.11.012","article-title":"Antifungal activity of silver nanoparticles and simvastatin against toxigenic species of Aspergillus","volume":"291","author":"Bocate","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s13568-019-0857-7","article-title":"Antifungal activity of silver nanoparticles in combination with ketoconazole against Malassezia furfur","volume":"9","author":"Mussin","year":"2019","journal-title":"AMB Express"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.matlet.2014.05.133","article-title":"Silver and silver oxide nanoparticles: Synthesis and characterization by thermal decomposition","volume":"130","author":"Ramezani","year":"2014","journal-title":"Mater. Lett."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"De Matteis, V., Cascione, M., Toma, C.C., and Leporatti, S. (2018). Silver Nanoparticles: Synthetic Routes, In Vitro Toxicity and Theranostic Applications for Cancer Disease. Nanomaterials (Basel), 8.","DOI":"10.3390\/nano8050319"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1080\/21691401.2018.1449118","article-title":"Role of gold and silver nanoparticles in cancer nano-medicine","volume":"46","author":"Chugh","year":"2018","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Wiemann, M., Vennemann, A., Blaske, F., Sperling, M., and Karst, U. (2017). Silver Nanoparticles in the Lung: Toxic Effects and Focal Accumulation of Silver in Remote Organs. Nanomaterials (Basel), 7.","DOI":"10.3390\/nano7120441"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4924","DOI":"10.1166\/jnn.2009.1269","article-title":"Distribution, translocation and accumulation of silver nanoparticles in rats","volume":"9","author":"Tang","year":"2009","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/1743-8977-10-36","article-title":"Biopersistence of silver nanoparticles in tissues from Sprague-Dawley rats","volume":"10","author":"Lee","year":"2013","journal-title":"Part. Fibre Toxicol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.nano.2007.02.001","article-title":"Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli","volume":"3","author":"Shahverdi","year":"2007","journal-title":"Nanomedicine"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.msec.2014.08.031","article-title":"Antimicrobial activity of the metals and metal oxide nanoparticles","volume":"44","author":"Dizaj","year":"2014","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1002\/chem.200400927","article-title":"Shape-controlled synthesis of metal nanostructures: The case of silver","volume":"11","author":"Wiley","year":"2005","journal-title":"Chem. A Eur. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4736","DOI":"10.1021\/cm020587b","article-title":"Uniform silver nanowires synthesis by reducing AgNO3 with ethylene glycol in the presence of seeds and poly (vinyl pyrrolidone)","volume":"14","author":"Sun","year":"2002","journal-title":"Chem. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s40824-020-00191-6","article-title":"Synthesis and characterization of a silver nanoparticle-containing polymer composite with antimicrobial abilities for application in prosthetic and orthotic devices","volume":"24","author":"Botero","year":"2020","journal-title":"Biomater. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1021\/cm0348949","article-title":"Synthesis and characterization of porous and nonporous monodisperse colloidal TiO2 particles","volume":"16","author":"Widoniak","year":"2004","journal-title":"Chem. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0021-9797(02)00205-9","article-title":"Preparation of highly concentrated stable dispersions of uniform silver nanoparticles","volume":"260","author":"Sondi","year":"2003","journal-title":"J. Colloid Interface Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3149","DOI":"10.1021\/am400044e","article-title":"Citrate-capped silver nanoparticles showing good bactericidal effect against both planktonic and sessile bacteria and a low cytotoxicity to osteoblastic cells","volume":"5","author":"Flores","year":"2013","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40824-019-0177-7","article-title":"Oleic acid conjugated polymeric photosensitizer for metastatic cancer targeting in photodynamic therapy","volume":"24","author":"Lee","year":"2020","journal-title":"Biomater. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.clae.2016.09.004","article-title":"Synthesis and characterization of nanosilver-silicone hydrogel composites for inhibition of bacteria growth","volume":"40","author":"Helaly","year":"2017","journal-title":"Contact Lens Anterior Eye"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s40580-017-0126-x","article-title":"In situ synthesis of silver nanoparticles on the surface of PDMS with high antibacterial activity and biosafety toward an implantable medical device","volume":"4","author":"Kim","year":"2017","journal-title":"Nano Converg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3395","DOI":"10.1021\/jp806683m","article-title":"Copolymers of maleic acid and their amphiphilic derivatives as stabilizers of silver nanoparticles","volume":"113","author":"Samoilova","year":"2009","journal-title":"J. Phys. Chem. B"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4400","DOI":"10.1021\/la903470f","article-title":"Role of citric acid in the formation of silver nanoplates through a synergistic reduction approach","volume":"26","author":"Jiang","year":"2010","journal-title":"Langmuir"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.jmst.2013.02.002","article-title":"A Novel Synthesis of Malic Acid Capped Silver Nanoparticles using Solanum lycopersicums Fruit Extract","volume":"29","author":"Umadevi","year":"2013","journal-title":"J. Mater. Sci. Technol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1550","DOI":"10.1016\/S1003-6326(09)60337-X","article-title":"Preparation of micro-sized and uniform spherical Ag powders by novel wet-chemical method","volume":"20","author":"Bing","year":"2010","journal-title":"Trans. Nonferrous Met. Soc. China"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"5015","DOI":"10.2147\/IJN.S113508","article-title":"Antimicrobial potential of green synthesized CeO2 nanoparticles from Olea europaea leaf extract","volume":"11","author":"Maqbool","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/2228-5326-2-9","article-title":"Chemical synthesis and antibacterial activity of novel-shaped silver nanoparticles","volume":"2","author":"Ha","year":"2012","journal-title":"Int. Nano Lett."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.poly.2017.05.046","article-title":"Pharmacological, electrochemical and drug\u2013DNA interaction aspects of tridentate Schiff bases and their triphenylphosphine nickel (II) complexes","volume":"133","author":"Shabbir","year":"2017","journal-title":"Polyhedron"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"79","DOI":"10.5455\/jice.20160124113632","article-title":"Biological synthesis of silver nanoparticles from Adansonia digitata L. fruit pulp extract, characterization, and its antimicrobial properties","volume":"5","author":"Kumar","year":"2016","journal-title":"J. Intercult. Ethnopharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2017\/9658598","article-title":"Evaluation of the antibacterial and antifungal properties of Phragmanthera capitata (Sprengel) Balle (Loranthaceae), a mistletoe growing on rubber tree, using the dilution techniques","volume":"2017","author":"Ohikhena","year":"2017","journal-title":"Sci. World J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1111\/j.1747-0285.2008.00668.x","article-title":"Di-and Triorganotin (IV) Esters of 3, 4-Methylenedioxyphenylpropenoic Acid: Synthesis, Spectroscopic Characterization and Biological Screening for Antimicrobial, Cytotoxic and Antitumor Activities","volume":"71","author":"Ahmad","year":"2008","journal-title":"Chem. Biol. Drug Des."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1007\/s12010-016-2077-9","article-title":"Effect of rol genes on polyphenols biosynthesis in Artemisia annua and their effect on antioxidant and cytotoxic potential of the plant","volume":"179","author":"Dilshad","year":"2016","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.sjbs.2015.09.006","article-title":"Green synthesis of silver nanoparticles using Andean blackberry fruit extract","volume":"24","author":"Kumar","year":"2017","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1007\/s13205-017-0670-4","article-title":"Green synthesis of silver oxide nanoparticles and its antibacterial activity against dental pathogens","volume":"7","author":"Manikandan","year":"2017","journal-title":"3 Biotech."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Raza, M.A., Kanwal, Z., Rauf, A., Sabri, A.N., Riaz, S., and Naseem, S. (2016). Size-and shape-dependent antibacterial studies of silver nanoparticles synthesized by wet chemical routes. Nanomaterials, 6.","DOI":"10.3390\/nano6040074"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Zhang, X.-F., Liu, Z.-G., Shen, W., and Gurunathan, S. (2016). Silver nanoparticles: Synthesis, characterization, properties, applications, and therapeutic approaches. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17091534"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1016\/j.matlet.2009.02.061","article-title":"Synthesis of silver particles with different sizes and morphologies","volume":"63","author":"Ruiz","year":"2009","journal-title":"Mater. Lett."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.bej.2015.02.019","article-title":"Robust and fluorescent silver nanoparticles using Artemisia annua: Biosynthesis, characterization and antibacterial activity","volume":"102","author":"Khatoon","year":"2015","journal-title":"Biochem. Eng. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1021\/nl010093y","article-title":"Crystalline silver nanowires by soft solution processing","volume":"2","author":"Sun","year":"2002","journal-title":"Nano Lett."},{"key":"ref_47","first-page":"1","article-title":"Antioxidant and antibacterial potential of silver nanoparticles: Biogenic synthesis utilizing apple extract","volume":"2016","author":"Nagaich","year":"2016","journal-title":"J. Pharm."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1750018","DOI":"10.1142\/S0219581X17500181","article-title":"Optimization of biosynthesis of silver oxide nanoparticles and its anticancer activity","volume":"16","author":"Karunagaran","year":"2017","journal-title":"Int. J. Nanosci."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1111\/j.1365-2672.2012.05253.x","article-title":"Silver nanoparticles: The powerful nanoweapon against multidrug-resistant bacteria","volume":"112","author":"Rai","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1002\/jps.24001","article-title":"Silver nanoparticles: Therapeutical uses, toxicity, and safety issues","volume":"103","author":"Rai","year":"2014","journal-title":"J. Pharm. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"46","DOI":"10.3109\/1040841X.2013.879849","article-title":"Metal nanoparticles: The protective nanoshield against virus infection","volume":"42","author":"Rai","year":"2016","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1477-3155-9-30","article-title":"Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds","volume":"9","author":"Lara","year":"2011","journal-title":"J. Nanobiotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.msec.2014.03.037","article-title":"Release of silver and copper nanoparticles from polyethylene nanocomposites and their penetration into Listeria monocytogenes","volume":"40","author":"Tamayo","year":"2014","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.joen.2013.08.022","article-title":"Evaluation of the antibacterial efficacy of silver nanoparticles against Enterococcus faecalis biofilm","volume":"40","author":"Wu","year":"2014","journal-title":"J. Endod."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1712","DOI":"10.1128\/AEM.02218-06","article-title":"Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli","volume":"73","author":"Pal","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3974","DOI":"10.1039\/C3RA44507K","article-title":"Size-controlled silver nanoparticles synthesized over the range 5\u2013100 nm using the same protocol and their antibacterial efficacy","volume":"4","author":"Agnihotri","year":"2014","journal-title":"RSC Adv."},{"key":"ref_57","unstructured":"World Health Organization (1998). Surface Decontamination of Fruits and Vegetables Eaten Raw: A Review, World Health Organization."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.tripleo.2011.05.023","article-title":"In vitro antimicrobial activity of maleic acid and ethylenediaminetetraacetic acid on endodontic pathogens","volume":"112","author":"Ballal","year":"2011","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"FMS79","DOI":"10.1111\/j.1365-2621.2004.tb13375.x","article-title":"Antimicrobial activity of citric, lactic, malic, or tartaric acids and nisin-incorporated soy protein film against Listeria monocytogenes, Escherichia coli O157: H7, and Salmonella gaminara","volume":"69","author":"Eswaranandam","year":"2004","journal-title":"J. Food Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/jfs.12025","article-title":"Antimicrobial activities of acetic acid, citric acid and lactic acid against S higella species","volume":"33","author":"In","year":"2013","journal-title":"J. Food Saf."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"8856","DOI":"10.3390\/molecules20058856","article-title":"Silver nanoparticles as potential antibacterial agents","volume":"20","author":"Franci","year":"2015","journal-title":"Molecules"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1073\/pnas.1115006109","article-title":"How Staphylococcus aureus biofilms develop their characteristic structure","volume":"109","author":"Periasamy","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.anaerobe.2011.03.023","article-title":"Quorum sensing in biofilms\u2013how to destroy the bacterial citadels or their cohesion\/power?","volume":"17","author":"Lazar","year":"2011","journal-title":"Anaerobe"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jphotobiol.2011.10.001","article-title":"The antimicrobial activity of photodynamic therapy against Streptococcus mutans using different photosensitizers","volume":"106","author":"Rolim","year":"2012","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1951","DOI":"10.1007\/s00253-013-5473-x","article-title":"Broad-spectrum bioactivities of silver nanoparticles: The emerging trends and future prospects","volume":"98","author":"Rai","year":"2014","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2346","DOI":"10.1088\/0957-4484\/16\/10\/059","article-title":"The bactericidal effect of silver nanoparticles","volume":"16","author":"Morones","year":"2005","journal-title":"Nanotechnology"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1128\/AEM.02001-07","article-title":"Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli","volume":"74","author":"Jung","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"R1385","DOI":"10.1152\/ajpregu.1999.277.5.R1385","article-title":"Mechanism of branchial apical silver uptake by rainbow trout is via the proton-coupled Na+ channel","volume":"277","author":"Bury","year":"1999","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1007\/s11051-008-9428-6","article-title":"Synthesis and antibacterial activity of silver nanoparticles with different sizes","volume":"10","author":"Ruiz","year":"2008","journal-title":"J. Nanopart. Res."},{"key":"ref_70","first-page":"1","article-title":"Effect of some organic acids on some fungal growth and their toxins production","volume":"2","author":"Hassan","year":"2015","journal-title":"Int. J. Adv. Biol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1002\/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO;2-3","article-title":"A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus","volume":"52","author":"Feng","year":"2000","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4061\/2011\/454090","article-title":"Biosynthesis of silver nanoparticles from Desmodium triflorum: A novel approach towards weed utilization","volume":"2011","author":"Ahmad","year":"2011","journal-title":"Biotechnol. Res. Int."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1080\/17458080.2015.1077534","article-title":"A novel approach for the biosynthesis of silver oxide nanoparticles using aqueous leaf extract of Callistemon lanceolatus (Myrtaceae) and their therapeutic potential","volume":"11","author":"Ravichandran","year":"2016","journal-title":"J. Exp. Nanosci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jgeb.2016.12.002","article-title":"Antimicrobial, antioxidant and antitumor activities of silver nanoparticles synthesized by Allium cepa extract: A green approach","volume":"15","author":"Gomaa","year":"2017","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1111\/dth.12262","article-title":"Topical malic acid in combination with citric acid: An option to treat recalcitrant warts","volume":"28","author":"Chiriac","year":"2015","journal-title":"Dermatol. Ther."},{"key":"ref_76","first-page":"4411","article-title":"Citric acid induces cell-cycle arrest and apoptosis of human immortalized keratinocyte cell line (HaCaT) via caspase-and mitochondrial-dependent signaling pathways","volume":"33","author":"Ying","year":"2013","journal-title":"Anticancer Res."},{"key":"ref_77","first-page":"1","article-title":"Citrate suppresses tumor growth in multiple models through inhibition of glycolysis, the tricarboxylic acid cycle and the IGF-1R pathway","volume":"7","author":"Ren","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"290","DOI":"10.4103\/1119-3077.181362","article-title":"The antifungal activity and cytotoxicity of silver containing denture base material","volume":"20","author":"Kurt","year":"2017","journal-title":"Niger. J. Clin. Pract."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"13608","DOI":"10.1021\/jp712087m","article-title":"Unique cellular interaction of silver nanoparticles: Size-dependent generation of reactive oxygen species","volume":"112","author":"Carlson","year":"2008","journal-title":"J. Phys. Chem. B"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1016\/j.tiv.2009.06.001","article-title":"Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells","volume":"23","author":"Kim","year":"2009","journal-title":"Toxicol. Vitr."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"9810","DOI":"10.1016\/j.biomaterials.2011.08.085","article-title":"The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles","volume":"32","author":"Park","year":"2011","journal-title":"Biomaterials"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"4382","DOI":"10.1039\/c1cc10357a","article-title":"Size dependent macrophage responses and toxicological effects of Ag nanoparticles","volume":"47","author":"Park","year":"2011","journal-title":"Chem. Commun."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1159\/000453446","article-title":"A novel approach of synthesizing and evaluating the anticancer potential of silver oxide nanoparticles in vitro","volume":"62","author":"Banerjee","year":"2017","journal-title":"Chemotherapy"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/j.tiv.2009.12.001","article-title":"Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism","volume":"24","author":"Park","year":"2010","journal-title":"Toxicol. Vitr."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1553","DOI":"10.1002\/smll.200900126","article-title":"Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes","volume":"5","author":"Yen","year":"2009","journal-title":"Small"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1007\/s11051-016-3624-6","article-title":"Effect of capping agents on the cytotoxicity of silver nanoparticles in human normal and cancer skin cell lines","volume":"18","author":"Netchareonsirisuk","year":"2016","journal-title":"J. Nanopart. Res."},{"key":"ref_87","first-page":"1","article-title":"Antitumor activity of colloidal silver on MCF-7 human breast cancer cells","volume":"29","year":"2010","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cis.2013.12.002","article-title":"Organic-coated silver nanoparticles in biological and environmental conditions: Fate, stability and toxicity","volume":"204","author":"Sharma","year":"2014","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Nguyen, K.C., Seligy, V.L., Massarsky, A., Moon, T.W., Rippstein, P., Tan, J., and Tayabali, A.F. (2013). Comparison of toxicity of uncoated and coated silver nanoparticles. Proceedings of Journal of Physics: Conference Series, IOP Publishing.","DOI":"10.1088\/1742-6596\/429\/1\/012025"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"246","DOI":"10.3762\/bjnano.7.23","article-title":"Surface coating affects behavior of metallic nanoparticles in a biological environment","volume":"7","author":"Capjak","year":"2016","journal-title":"Beilstein J. Nanotechnol."},{"key":"ref_91","first-page":"899","article-title":"Green synthesis of silver nanoparticles using Artemisia annua and Sida acuta leaves extract and their antimicrobial, antioxidant and corrosion inhibition potentials","volume":"5","author":"Johnson","year":"2014","journal-title":"J. Mater. Environ. Sci."},{"key":"ref_92","first-page":"2053","article-title":"Effect of salicylic acid, malic acid, citric acid and sucrose on antioxidant activity, membrane stability and ACC-oxidase activity in relation to vase life of carnation cut flowers","volume":"8","author":"Kazemi","year":"2012","journal-title":"J. Agric. Technol."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/11\/4\/76\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:26:44Z","timestamp":1760178404000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/11\/4\/76"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,23]]},"references-count":92,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["jfb11040076"],"URL":"https:\/\/doi.org\/10.3390\/jfb11040076","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,23]]}}}