{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:30:21Z","timestamp":1791646221061,"version":"4.3.4"},"reference-count":40,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,11,15]],"date-time":"2020-11-15T00:00:00Z","timestamp":1605398400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Calcium phosphates (CPs) used as biomaterials have been intensively studied in recent years. In most studies, the determination of the chemical composition is mandatory. Due to the versatility and possibilities of performing qualitative and quantitative compositional analyses, energy dispersive spectrometry (EDS) is a widely used technique in this regard. The range of calcium phosphates is very diverse, the first method of approximating the type of compound being EDS microanalysis, by assessing the atomic Ca\/P ratio. The value of this ratio can be influenced by several factors correlated with instrumental parameters and analysed samples. This article highlights the influence of the electron beam acceleration voltage (1 kV\u201330 kV) and of the particle size of calcium phosphate powders on the EDS analysis results. The characterised powders were obtained from bovine bones heat-treated at 1200 \u00b0C for 2 h, which have been ground and granulometrically sorted by mechanical vibration. The granulometric sorting generated three types of samples, with particle sizes &lt; 20 \u03bcm, &lt; 40 \u03bcm and &lt; 100 \u03bcm, respectively. These were morphologically and dimensionally analysed by scanning electron microscopy (SEM) and compositionally by EDS, after the spectrometer was calibrated with a standard reference material (SRM) from NIST (National Institute of Standards and Technology). The results showed that the adjusting of acceleration voltage and of the powder particle size significantly influences the spectrum profile and the results of EDS analyses, which can lead to an erroneous primary identification of the analysed calcium phosphate type.<\/jats:p>","DOI":"10.3390\/jfb11040082","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T11:04:20Z","timestamp":1605524660000},"page":"82","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Considerations and Influencing Parameters in EDS Microanalysis of Biogenic Hydroxyapatite"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6757-5616","authenticated-orcid":false,"given":"Florin","family":"Miculescu","sequence":"first","affiliation":[{"name":"Faculty of Materials Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Lu\u021b\u0103","sequence":"additional","affiliation":[{"name":"Faculty of Materials Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8430-8931","authenticated-orcid":false,"given":"Andreea Elena","family":"Constantinescu","sequence":"additional","affiliation":[{"name":"Faculty of Materials Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5886-6478","authenticated-orcid":false,"given":"Andreea","family":"Maidaniuc","sequence":"additional","affiliation":[{"name":"S.C. Nuclear NDT Research &amp; Services S.R.L, 104 Berceni St., Central Laboratory Building, 041919 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aura-C\u0103t\u0103lina","family":"Mocanu","sequence":"additional","affiliation":[{"name":"Faculty of Materials Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2723-6713","authenticated-orcid":false,"given":"Marian","family":"Miculescu","sequence":"additional","affiliation":[{"name":"Faculty of Materials Science and Engineering, University Politehnica of Bucharest, 313 Splaiul Independentei, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7191-7471","authenticated-orcid":false,"given":"\u0218tefan Ioan","family":"Voicu","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry and Environmental Engineering, University Politehnica of Bucharest, 011061 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucian Toma","family":"Ciocan","sequence":"additional","affiliation":[{"name":"Department of Prosthetics Technology and Dental Materials, University of Medicine and Pharmacy, 020022 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,15]]},"reference":[{"key":"ref_1","first-page":"335","article-title":"A study on the influence of the primary electron beam on nanodimensional layer analysis","volume":"6","author":"Miculescu","year":"2011","journal-title":"Dig. 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