{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T06:06:32Z","timestamp":1769061992403,"version":"3.49.0"},"reference-count":64,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Despite the advent of promising technologies in tissue engineering, finding a biomimetic 3D bio-construct capable of enhancing cell attachment, maintenance, and function is still a challenge in producing tailorable scaffolds for bone regeneration. Here, osteostimulatory effects of the butterfly wings as a naturally porous and non-toxic chitinous scaffold on mesenchymal stromal cells are assessed. The topographical characterization of the butterfly wings implied their ability to mimic bone tissue microenvironment, whereas their regenerative potential was validated after a 14-day cell culture. In vivo analysis showed that the scaffold induced no major inflammatory response in Wistar rats. Topographical features of the bioconstruct upregulated the osteogenic genes, including COL1A1, ALP, BGLAP, SPP1, SP7, and AML3 in differentiated cells compared to the cells cultured in the culture plate. However, butterfly wings were shown to provide a biomimetic microstructure and proper bone regenerative capacity through a unique combination of various structural and material properties. Therefore, this novel platform can be confidently recommended for bone tissue engineering applications.<\/jats:p>","DOI":"10.3390\/jfb13020068","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T03:33:18Z","timestamp":1654054398000},"page":"68","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Crossing Phylums: Butterfly Wing as a Natural Perfusable Three-Dimensional (3D) Bioconstruct for Bone Tissue Engineering"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4922-6628","authenticated-orcid":false,"given":"Fatemeh","family":"Mostofi","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran 19839-69411, Iran"},{"name":"Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, National Cell Bank, Tehran 13169-43551, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2574-9758","authenticated-orcid":false,"given":"Marzieh","family":"Mostofi","sequence":"additional","affiliation":[{"name":"Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, National Cell Bank, Tehran 13169-43551, Iran"},{"name":"Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran 31979-37551, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Behnaz","family":"Niroomand","sequence":"additional","affiliation":[{"name":"Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, National Cell Bank, Tehran 13169-43551, Iran"},{"name":"School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran 19839-63113, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2411-7903","authenticated-orcid":false,"given":"Saadi","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, National Cell Bank, Tehran 13169-43551, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atefeh","family":"Alipour","sequence":"additional","affiliation":[{"name":"Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran 13169-43551, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2426-6690","authenticated-orcid":false,"given":"Shahin","family":"Homaeigohar","sequence":"additional","affiliation":[{"name":"School of Science and Engineering, University of Dundee, Dundee DD1 4HN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javad","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Life Science, Faculty of New Science and Technology, University of Tehran, Tehran 14399-57131, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Ali","family":"Shokrgozar","sequence":"additional","affiliation":[{"name":"Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, National Cell Bank, Tehran 13169-43551, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8123-0857","authenticated-orcid":false,"given":"Hosein","family":"Shahsavarani","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran 19839-69411, Iran"},{"name":"Laboratory of Regenerative Medicine and Biomedical Innovations, Pasteur Institute of Iran, National Cell Bank, Tehran 13169-43551, Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Niinikoski, J., and Hunt, T. 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