{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T21:17:41Z","timestamp":1773263861016,"version":"3.50.1"},"reference-count":19,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,7,31]],"date-time":"2022-07-31T00:00:00Z","timestamp":1659225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fulbright Senior Grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Polymeric nanoparticles have been introduced as a delivery vehicle for active compounds in a broad range of medical applications due to their biocompatibility, stability, controlled release of active compounds, and reduced toxicity. The oral route is the most used approach for delivery of biologics to the body. The homeostasis and function of oral cavity tissues are dependent on the activity of stem cells. The present work focuses, for the first time, on the interaction between two types of polymeric nanoparticles, poly (lactic-co-glycolic acid) or PLGA and PLGA\/chitosan, and two stem cell populations, oral keratinocyte stem cells (OKSCs) and stem cells from human exfoliated deciduous teeth (SHEDs). The main results show that statistical significance was observed in OKSCs uptake when compared with normal keratinocytes and transit amplifying cells after 24 h of incubation with 5 and 10 \u00b5g\/mL PLGA\/chitosan. The CD117+ SHED subpopulation incorporated more PLGA\/chitosan nanoparticles than nonseparated SHED. The uptake for PLGA\/chitosan particles was better than for PLGA particles with longer incubation times, yielding better results in both cell types. The present results demonstrate that nanoparticle uptake depends on stem cell type, incubation time, particle concentration, and surface properties.<\/jats:p>","DOI":"10.3390\/jfb13030109","type":"journal-article","created":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T02:06:42Z","timestamp":1659319602000},"page":"109","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["PLGA Nanoparticles Uptake in Stem Cells from Human Exfoliated Deciduous Teeth and Oral Keratinocyte Stem Cells"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5202-4657","authenticated-orcid":false,"given":"Maria","family":"Tizu","sequence":"first","affiliation":[{"name":"Centre for Immunogenetics and Virology, Fundeni Clinical Institute, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Road, 022328 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4284-8654","authenticated-orcid":false,"given":"Ion","family":"M\u0103run\u021belu","sequence":"additional","affiliation":[{"name":"Centre for Immunogenetics and Virology, Fundeni Clinical Institute, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Road, 022328 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bogdan Mihai","family":"Cristea","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Carol Davila University of Medicine and Pharmacy, 8 Blvd Eroii Sanitari, 050474 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudiu","family":"Nistor","sequence":"additional","affiliation":[{"name":"Central Military Hospital, Carol Davila University of Medicine and Pharmacy, 134 Stefan Furtuna Street, 010899 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikolay","family":"Ishkitiev","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Medical Univerity of Sofia, Blvd Akademik Ivan Evstratiev Geshov 15, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zornitsa","family":"Mihaylova","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Medical Univerity of Sofia, Blvd Akademik Ivan Evstratiev Geshov 15, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rozaliya","family":"Tsikandelova","sequence":"additional","affiliation":[{"name":"Biosciences Institute, Newcastle University, Catherine Cookson Building, Newcastle upon Tyne NE2 4HH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Miteva","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Medical Univerity of Sofia, Blvd Akademik Ivan Evstratiev Geshov 15, 1431 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4200-3069","authenticated-orcid":false,"given":"Ana","family":"Caruntu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, \u201cCarol Davila\u201d Central Military Emergency Hospital, 010825 Bucharest, Romania"},{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, \u201cTitu Maiorescu\u201d University, 031593 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Sabliov","sequence":"additional","affiliation":[{"name":"Biological and Agricultural Engineering Department, Louisiana State University and LSU Agricultural Center, 141 E. 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Doran Bldg, Baton Rouge, LA 70803, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bogdan","family":"Calenic","sequence":"additional","affiliation":[{"name":"Centre for Immunogenetics and Virology, Fundeni Clinical Institute, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Road, 022328 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ileana","family":"Constantinescu","sequence":"additional","affiliation":[{"name":"Centre for Immunogenetics and Virology, Fundeni Clinical Institute, Carol Davila University of Medicine and Pharmacy, 258 Fundeni Road, 022328 Bucharest, Romania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1701035","DOI":"10.1002\/adhm.201701035","article-title":"Functionalizing Plga and PLGA Derivatives for DrugDelivery and Tissue Regeneration Applications","volume":"7","author":"Martins","year":"2017","journal-title":"Adv. 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