{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T03:25:29Z","timestamp":1779247529395,"version":"3.51.4"},"reference-count":33,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T00:00:00Z","timestamp":1663027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tissue Bank of France (TBF)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>A preclinical study was performed to investigate the efficacy and safety of a new viral inactivated, devitalized, freeze-dried and gamma-sterilized human umbilical cord amniotic membrane (lhUC-AM) for the treatment of deep scleral and corneal defects with or without perforation. Firstly, lhUC-AM was investigated on experimental deep sclerectomy in rabbit eyes (n = 12) and compared to autograft (n = 4) on cross section histology. Secondly, lhUC-AM was studied on a selected series of uncontrolled cases of corneal defects (n = 18) with or without perforation, in dogs and cats. lhUC-AM tolerance, reconstruction of the deep corneal lesion and recovery of the structural aspect of the tissue were followed post-surgery. In experimental deep sclerectomy, histology showed that the lhUC-AM was well tolerated and degraded completely in 45 days while allowing an overall quality and kinetic of scleral regeneration, similar to autograft. In the clinical situations, lhUC-AM was well tolerated, with ocular inflammatory signs quickly decreasing after surgery. Mean follow-up was 16.40 \u00b1 11.43 months. In 15 out of 18 cases, lhUC-AM allowed ocular surface wound healing. The ocular surface was fully reconstructed three months after surgery. This study suggests a good safety and efficacy profile of lhUC-AM in the treatment of deep corneal or scleral defect in animals. This new tissue should now facilitate the treatment of severe ocular surface diseases in humans.<\/jats:p>","DOI":"10.3390\/jfb13030150","type":"journal-article","created":{"date-parts":[[2022,9,13]],"date-time":"2022-09-13T22:37:28Z","timestamp":1663108648000},"page":"150","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["New Devitalized Freeze-Dried Human Umbilical Cord Amniotic Membrane as an Innovative Treatment of Ocular Surface Defects: Preclinical Results"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3015-8648","authenticated-orcid":false,"given":"Sophie","family":"Cognard","sequence":"first","affiliation":[{"name":"Clinique V\u00e9t\u00e9rinaire des Ducs de Bourgogne, 11 Ter Rue Paul Langevin, 21300 Chenove, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence","family":"Barnouin","sequence":"additional","affiliation":[{"name":"Tissue Bank of France, 6 rue d\u2019Italie, 69780 Mions, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justine","family":"Bosc","sequence":"additional","affiliation":[{"name":"Tissue Bank of France, 6 rue d\u2019Italie, 69780 Mions, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3463-6093","authenticated-orcid":false,"given":"Florelle","family":"Gindraux","sequence":"additional","affiliation":[{"name":"Service de Chirurgie Orthop\u00e9dique, Traumatologique et Plastique, CHU Besan\u00e7on, F-25000 Besan\u00e7on, France"},{"name":"Laboratoire de Nanom\u00e9decine, Imagerie, Th\u00e9rapeutique EA 4662, Universit\u00e9 Bourgogne Franche-Comt\u00e9, F-25000 Besan\u00e7on, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Claire","family":"Robin","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Universit\u00e9 de Toulouse, ENVT, 31300 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0426-4957","authenticated-orcid":false,"given":"Jean-Yves","family":"Douet","sequence":"additional","affiliation":[{"name":"Small Animal Clinic, Universit\u00e9 de Toulouse, ENVT, 31300 Toulouse, France"},{"name":"IHAP, Universit\u00e9 de Toulouse, INRAE, ENVT, 31300 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Thuret","sequence":"additional","affiliation":[{"name":"Biology, Engineering and IMAGING of Corneal Graft, BiiGC, Faculty of Medicine, Health Innovation Campus, 10 rue de la Marandi\u00e8re, 42270 Saint-Priest en Jarez, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/S0195-5616(97)50104-5","article-title":"Surgery of the cornea","volume":"27","author":"Wilkie","year":"1997","journal-title":"Vet. 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