{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T06:50:43Z","timestamp":1762066243628,"version":"build-2065373602"},"reference-count":34,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T00:00:00Z","timestamp":1663200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Ministry of Economy, Trade and Industry","award":["2004-5"],"award-info":[{"award-number":["2004-5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>This clinical report describes the immediate autograft of primary (milk) teeth-derived demineralized dentin matrix (DDM) granules for a 6-year-old boy with unilateral alveolar cleft. First, four primary teeth were extracted, crushed in an electric mill for 1 min, and the crushed granules were demineralized in 2% HNO3 solution for 20 min. Simultaneously, the nasal mucoperiosteum was pushed upwards above the apices of the permanent central incisor adjacent to the cleft. The nasal and palatal openings were closed by suturing the mucoperiosteum on both sides of the cleft with absorbable threads. The wet DDM granules were grafted into the managed cleft triangle space, and a labial flap was repositioned. The radiographic images at 6 months showed the continuous hard tissues in the cleft area and DDM granules onto lateral incisor (22) and impacted canine (23). The 3D-CT views at 2 years showed impacted tooth (22) blocked by primary canine and the replacement of DDM granules by bone near teeth (22,23). At 4 years, tooth crown (22) was situated just under the mucous membrane, and teeth (22,23) erupted spontaneously until 6 years without a maxillary expansion and a tow guidance of canine. The DDM granules contributed to bone formation without the inhibition of spontaneous tooth eruption. We concluded that autogenous primary teeth DDM graft should become a minimally invasive procedure without bone harvesting and morbidities for unilateral alveolar cleft.<\/jats:p>","DOI":"10.3390\/jfb13030153","type":"journal-article","created":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T01:35:10Z","timestamp":1663292110000},"page":"153","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Primary Teeth-Derived Demineralized Dentin Matrix Autograft for Unilateral Maxillary Alveolar Cleft during Mixed Dentition"],"prefix":"10.3390","volume":"13","author":[{"given":"Yusuke","family":"Matsuzawa","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan"},{"name":"Division of Oral and Maxillofacial Surgery, Keiyukai Sapporo Hospital, Sapporo 003-0026, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0233-9839","authenticated-orcid":false,"given":"Naoto","family":"Okubo","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Medicine, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-8586, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soichi","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruhiko","family":"Kashiwazaki","sequence":"additional","affiliation":[{"name":"Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimasa","family":"Kitagawa","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis and Medicine, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1831-3454","authenticated-orcid":false,"given":"Yoichi","family":"Ohiro","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Mikoya","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Graduate School of Dental Medicine, Hokkaido University, Sapporo 060-8586, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2740-0413","authenticated-orcid":false,"given":"Toshiyuki","family":"Akazawa","sequence":"additional","affiliation":[{"name":"Industrial Technology and Environment Research Development, Hokkaido Research Organization, Sapporo 060-0819, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6442-9266","authenticated-orcid":false,"given":"Masaru","family":"Murata","sequence":"additional","affiliation":[{"name":"Division of Oral Regenerative Medicine, School of Dentistry, Health Sciences University of Hokkaido, Tobetsu 061-0293, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,15]]},"reference":[{"key":"ref_1","first-page":"87","article-title":"Secondary bone grafting of residual and palatal clefts","volume":"30","author":"Boyne","year":"1972","journal-title":"J. Oral Surg."},{"key":"ref_2","first-page":"174","article-title":"Secondary bone graft and eruption of the permanent canine in patients with alveolar clefts: Literature review and case report","volume":"70","author":"Teles","year":"2000","journal-title":"Angle Orthod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1597\/09-172","article-title":"Monocortical mandibular bone grafting for reconstruction of alveolar cleft","volume":"47","author":"Mikoya","year":"2010","journal-title":"Cleft Palate Craniofac. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1097\/SCS.0000000000001132","article-title":"Chin bone graft for maxillary alveolar cleft: Indications and limitations","volume":"25","author":"Rahpeym","year":"2014","journal-title":"J Craniofac Surg."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Pignatello, R. (2013). Autograft of dentin materials for bone regeneration. Advances in Biomaterials Science and Biomedical Applications, Intech Publisher.","DOI":"10.5772\/56420"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1126\/science.150.3698.893","article-title":"Bone: Formation by autoinduction","volume":"12","author":"Urist","year":"1965","journal-title":"Science"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1001\/archsurg.1967.01330120035008","article-title":"Bone induction in excavation chambers in matrix of decalcified dentin","volume":"94","author":"Bang","year":"1964","journal-title":"Arch. Surg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/0003-9969(67)90095-7","article-title":"Bone induction by decalcified dentine implanted into oral, osseous and muscle tissues","volume":"12","author":"Yeomans","year":"1964","journal-title":"Arch. Oral. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Shakya, M., Murata, M., Yokozeki, K., Akazawa, T., Nagayasu, H., Adhikari, B.R., and Upadhyaya, C. (2021). Accelerated bone induction of adult rat compact bone plate scratched by ultrasonic scaler using acidic electrolyzed water. Materials, 17.","DOI":"10.3390\/ma14123347"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Murata, M., Okubo, N., Shakya, M., Kabir, M.A., Yokozeki, K., Zhu, B.W., Ishikawa, M., Kitamura, R., and Akazawa, T. (2019). Dentin materials as biological scaffolds for tissue engineering. Biomaterial-Supported Tissue Reconstruction or Regeneration, IntechOpen.","DOI":"10.5772\/intechopen.85452"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"442","DOI":"10.2109\/jcersj2.118.442","article-title":"Autograft of human tooth and demineralized dentin matrices for bone augmentation","volume":"118","author":"Tazaki","year":"2010","journal-title":"J. Ceram. Soc. Jpn."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"363","DOI":"10.2485\/jhtb.23.363","article-title":"Radiological evaluation of human dentin autografts in Bangladesh","volume":"23","author":"Kabir","year":"2014","journal-title":"J. Hard Tissue Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1111\/cid.12667","article-title":"Autogenous DDM versus Bio-Oss granules in GBR for immediate implantation in periodontal postextraction sites: A prospective clinical study","volume":"20","author":"Li","year":"2018","journal-title":"Clin. Implant. Dent. Relat. Res."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Um, I.W., Ku, J.K., Kim, Y.M., Yun, P.Y., Chang, N.H., Kim, Y.K., and Choi, Y. (2020). Allogenic demineralized dentin matrix graft for guided bone regeneration in dental implants. Appl. Sci., 10.","DOI":"10.3390\/app10134661"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1563\/aaid-joi-D-19-00315","article-title":"Full regeneration of maxillary alveolar bone using autogenous partially demineralized dentin matrix and particulate cancellous bone and marrow for implant-supported full arch rehabilitation","volume":"1","author":"Umebayashi","year":"2020","journal-title":"J. Oral. Implantol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"47","DOI":"10.2485\/jhtb.31.47","article-title":"Autograft of demineralized dentin matrix prepared immediately after extraction for horizontal bone augmentation of the anterior atrophic maxilla: A first case of non-vital tooth-derived dentin","volume":"31","author":"Okubo","year":"2022","journal-title":"J. Hard Tissue Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"37","DOI":"10.5125\/jkaoms.2014.40.1.37","article-title":"Familial tooth bone graft for ridge and sinus augmentation: A report of two cases","volume":"40","author":"Kim","year":"2014","journal-title":"J. Korean Assoc. Oral Maxillofac. Surg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.ijom.2017.02.1279","article-title":"Clinical application of autogenous partially demineralized dentin matrix prepared immediately after extraction for alveolar bone regeneration in implant dentistry: A pilot study","volume":"47","author":"Minamizato","year":"2018","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Murata, M., Kabir, M.A., Hirose, Y., Ochi, M., Okubo, N., Akazawa, T., and Kashiwazaki, H. (2022). Histological evidences of autograft of dentin\/cementum granules into unhealed socket at 5 months after tooth extraction for implant placement. J. Funct. Biomater., 25.","DOI":"10.3390\/jfb13020066"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Murata, M., Nakanishi, Y., Kusano, K., Hirose, Y., Tazaki, J., Akazawa, T., and Mizoguchi, I. (2022). Immediate tooth autotransplantation with root canal filling and partially demineralized dentin\/cementum matrix into congenital Missing tooth region. J Funct Biomater., 16.","DOI":"10.3390\/jfb13020082"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/j.injury.2011.06.015","article-title":"Complications following autologous bone graft harvesting from the iliac crest and using the RIA: A systematic review","volume":"42","author":"Dimitriou","year":"2011","journal-title":"Injury"},{"key":"ref_22","first-page":"B243","article-title":"Autogenous demineralized dentin matrix for maxillary sinus augmentation in humans: The first clinical report","volume":"82","author":"Murata","year":"2003","journal-title":"J. Dent. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Koga, T., Minamizato, T., Kawai, Y., Miura, K., Nakatani, Y., Sumita, Y., and Asahina, I. (2016). Bone regeneration using dentin matrix depends on the degree of demineralization and particle size. PLoS ONE., 11.","DOI":"10.1371\/journal.pone.0147235"},{"key":"ref_24","unstructured":"Murata, M., and Um, I.W. (2014). Familial tooth bone graft. Advances in Oral Tissue Engineering, Quintessence Publishing Co., Inc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1597\/1545-1569_2001_038_0155_mbgmfr_2.0.co_2","article-title":"Mandibular bone graft material for reconstruction of alveolar cleft defects: Long-term results","volume":"38","author":"Enemark","year":"2001","journal-title":"Cleft Palate Craniofac. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1597\/03-158.1","article-title":"Morbidity of chin bone transplants used for reconstructing alveolar defects in cleft patients","volume":"42","author":"Booij","year":"2005","journal-title":"Cleft Palate Craniofac. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"85","DOI":"10.5125\/jkaoms.2022.48.2.85","article-title":"Cone-beam computed tomographic comparison of chin symphysis bone particles and allograft versus iliac crest bone graft alone for reconstruction of alveolar bone defects in cleft patients","volume":"30","author":"Attar","year":"2022","journal-title":"J. Korean Assoc. Oral Maxillofac. Surg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.ijom.2005.12.007","article-title":"Alveolar cleft osteoplasty using tissue-engineered osteogenic material","volume":"35","author":"Hibi","year":"2006","journal-title":"Int. J. Oral Maxillofac. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e65","DOI":"10.1097\/SCS.0b013e3182700997","article-title":"Bone transport and bone graft using auto-tooth bone for alveolar cleft repair","volume":"24","author":"Hara","year":"2013","journal-title":"J. Craniofac. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1097\/PRS.0000000000003686","article-title":"Three-dimensional cone beam computed tomography volumetric outcomes of rhBMP-2\/demineralized bone matrix versus iliac crest bone graft for alveolar cleft reconstruction","volume":"140","author":"Liang","year":"2017","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"318e","DOI":"10.1097\/PRS.0000000000003519","article-title":"A comparative analysis of recombinant human bone morphogenetic protein-2 with a demineralized bone matrix versus iliac crest bone graft for secondary alveolar bone grafts in patients with cleft lip and palate: Review of 501 cases","volume":"140","author":"Hammoudeh","year":"2017","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2462","DOI":"10.1016\/j.joms.2021.09.017","article-title":"A follow-up study on the clinical outcomes of alveolar reconstruction using octacalcium phosphate granules and atelocollagen complex","volume":"79","author":"Kibe","year":"2021","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1002\/jbm.b.30531","article-title":"Octacalcium phosphate combined with collagen orthotopically enhances bone regeneration","volume":"79","author":"Kamakura","year":"2006","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e3611","DOI":"10.1097\/GOX.0000000000003611","article-title":"Bone morphogenetic protein-2 and demineralized bone matrix in difficult bony reconstructions in cleft patients","volume":"22","author":"Makar","year":"2021","journal-title":"Plast. Reconstr. Surg. Glob. Open."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/13\/3\/153\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:32:08Z","timestamp":1760142728000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/13\/3\/153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,15]]},"references-count":34,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["jfb13030153"],"URL":"https:\/\/doi.org\/10.3390\/jfb13030153","relation":{},"ISSN":["2079-4983"],"issn-type":[{"type":"electronic","value":"2079-4983"}],"subject":[],"published":{"date-parts":[[2022,9,15]]}}}