{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T11:40:18Z","timestamp":1791632418296,"version":"4.3.4"},"reference-count":51,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,8]],"date-time":"2023-06-08T00:00:00Z","timestamp":1686182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","award":["59307082\u2014TRR67"],"award-info":[{"award-number":["59307082\u2014TRR67"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","award":["497439310"],"award-info":[{"award-number":["497439310"]}]},{"name":"TU Dresden \u201cZukunftskonzept\u201d","award":["59307082\u2014TRR67"],"award-info":[{"award-number":["59307082\u2014TRR67"]}]},{"name":"TU Dresden \u201cZukunftskonzept\u201d","award":["497439310"],"award-info":[{"award-number":["497439310"]}]},{"name":"funds of the excellence initiative of the federal and state governments of Germany","award":["59307082\u2014TRR67"],"award-info":[{"award-number":["59307082\u2014TRR67"]}]},{"name":"funds of the excellence initiative of the federal and state governments of Germany","award":["497439310"],"award-info":[{"award-number":["497439310"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Biopolymer hydrogels have become an important group of biomaterials in experimental and clinical use. However, unlike metallic or mineral materials, they are quite sensitive to sterilization. The aim of this study was to compare the effects of gamma irradiation and supercritical carbon dioxide (scCO2) treatment on the physicochemical properties of different hyaluronan (HA)- and\/or gelatin (GEL)-based hydrogels and the cellular response of human bone marrow-derived mesenchymal stem cells (hBMSC). Hydrogels were photo-polymerized from methacrylated HA, methacrylated GEL, or a mixture of GEL\/HA. The composition and sterilization methods altered the dissolution behavior of the biopolymeric hydrogels. There were no significant differences in methacrylated GEL release but increased methacrylated HA degradation of gamma-irradiated samples. Pore size\/form remained unchanged, while gamma irradiation decreased the elastic modulus from about 29 kPa to 19 kPa compared to aseptic samples. HBMSC proliferated and increased alkaline phosphatase activity (ALP) particularly in aseptic and gamma-irradiated methacrylated GEL\/HA hydrogels alike, while scCO2 treatment had a negative effect on both proliferation and osteogenic differentiation. Thus, gamma-irradiated methacrylated GEL\/HA hydrogels are a promising base for multi-component bone substitute materials.<\/jats:p>","DOI":"10.3390\/jfb14060317","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T02:03:18Z","timestamp":1686276198000},"page":"317","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Effects of Gamma Irradiation and Supercritical Carbon Dioxide Sterilization on Methacrylated Gelatin\/Hyaluronan Hydrogels"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1343-4938","authenticated-orcid":false,"given":"Christiane","family":"Heinemann","sequence":"first","affiliation":[{"name":"Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universit\u00e4t Dresden, Budapester Str. 27, D-01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frauke","family":"Buchner","sequence":"additional","affiliation":[{"name":"Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universit\u00e4t Dresden, Budapester Str. 27, D-01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2285-3621","authenticated-orcid":false,"given":"Poh Soo","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universit\u00e4t Dresden, Budapester Str. 27, D-01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1903-1929","authenticated-orcid":false,"given":"Anne","family":"Bernhardt","sequence":"additional","affiliation":[{"name":"Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine, Technische Universit\u00e4t Dresden, D-01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0659-0238","authenticated-orcid":false,"given":"Benjamin","family":"Kruppke","sequence":"additional","affiliation":[{"name":"Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universit\u00e4t Dresden, Budapester Str. 27, D-01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Peter","family":"Wiesmann","sequence":"additional","affiliation":[{"name":"Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universit\u00e4t Dresden, Budapester Str. 27, D-01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5611-9903","authenticated-orcid":false,"given":"Vera","family":"Hintze","sequence":"additional","affiliation":[{"name":"Institute of Materials Science, Max Bergmann Center of Biomaterials, Technische Universit\u00e4t Dresden, Budapester Str. 27, D-01069 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3904","DOI":"10.1016\/j.bioactmat.2021.03.040","article-title":"Biofabrication of Natural Hydrogels for Cardiac, Neural, and Bone Tissue Engineering Applications","volume":"6","author":"Elkhoury","year":"2021","journal-title":"Bioact. 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