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Corneal transplantation is currently considered to be one of the common treatments for vision loss. This paper presents a novel approach utilizing dual-crosslinked membranes composed of polyrotaxane multiple aldehydes (PRAs), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and N-hydroxysuccinimide (NHS) in the development process. Collagen was crosslinked, respectively, by EDC\/NHS and PRAs to form stable amide bonds and imine groups. Through the formation of a double interpenetrating network, dual-crosslinked (Col-EDC-PRA) membranes exhibited enhanced resistance to collagenase degradation and superior mechanical properties compared to membranes crosslinked with a single crosslinker. Furthermore, Col-EDC-PRA membranes display favorable light transmittance and water content characteristics. Cell experiments showed that Col-EDC-PRA membranes were noncytotoxic and were not significantly different from other membranes. In a rabbit keratoplasty model, corneal stromal repair occurred at 5 months, evidenced by the presence of stromal cells and neo-stroma, as depicted in hematoxylin\u2013eosin-stained histologic sections and optical coherence tomography images of the anterior segment. Moreover, there was no inflammation and corneal neovascularization, as well as no corneal rejection reaction in the surgical area. Overall, the results demonstrated that the dual-crosslinked membranes served effectively for corneal tissue regeneration after corneal defect.<\/jats:p>","DOI":"10.3390\/jfb14070360","type":"journal-article","created":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T01:42:58Z","timestamp":1689039778000},"page":"360","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Properties of Dual-Crosslinked Collagen-Based Membranes as Corneal Repair Material"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1313-334X","authenticated-orcid":false,"given":"Lulu","family":"Wang","sequence":"first","affiliation":[{"name":"Henan Provincial People\u2019s Hospital, Henan Eye Hospital, Zhengzhou University People\u2019s Hospital, Zhengzhou 450003, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuehai","family":"Peng","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China"},{"name":"Guangzhou Proud Seeing Biotechnology Co., Ltd., Guangzhou 510623, China"},{"name":"School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfang","family":"Liu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China"},{"name":"School of Materials Science and Engineering, South China University of Technology, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Ren","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China"},{"name":"School of Materials Science and Engineering, South China University of Technology, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,10]]},"reference":[{"key":"ref_1","first-page":"214","article-title":"Corneal blindness: A global perspective","volume":"79","author":"Whitcher","year":"2001","journal-title":"Bull. 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