{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:29:30Z","timestamp":1760149770978,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T00:00:00Z","timestamp":1694995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET)","award":["322096-5"],"award-info":[{"award-number":["322096-5"]}]},{"name":"Ministry of Agriculture, Food and Rural Affairs (MAFRA)","award":["322096-5"],"award-info":[{"award-number":["322096-5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Blood group mismatch in veterinary medicine is a significant problem in blood transfusion, sometimes leading to severe transfusion reactions and even patient death. Blood groups vary from species to species and there are three known blood groups in cats: A, B and AB. While A-type cats are most common, there is a shortage of feline B-type blood groups in cats. By using methoxy polyethylene glycol (mPEG) to protect antigenic epitopes on red blood cells (RBCs), we aimed to find the optimal conditions for the production of feline universal RBCs. The surfaces of feline A-type RBCs were treated with mPEG at various molecular weights and concentrations. Agglutination tests showed that the coating of feline A-type RBCs with mPEG of 20 kDa and 2 mM blocked hemagglutination to feline anti-A alloantibodies over 8 h. While no differences in RBC size and shape between intact and mPEG-treated RBCs were seen, coating RBCs with mPEG inhibited the binding of feline anti-A alloantibodies. Furthermore, the mPEG-treated RBCs did not cause spontaneous hemolysis or osmotic fragility, compared to control RBCs. According to a monocyte monolayer assay, mPEG treatment significantly reduced feline anti-A antibody-mediated phagocystosis of RBCs. These results confirm the potential of using activated mPEG on feline A-type RBC to create universal erythrocytes for transfusion to B-type cats.<\/jats:p>","DOI":"10.3390\/jfb14090476","type":"journal-article","created":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T23:32:27Z","timestamp":1694993547000},"page":"476","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Production of Feline Universal Erythrocytes with Methoxy Polyethylene Glycol"],"prefix":"10.3390","volume":"14","author":[{"given":"Hyung Kyu","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"},{"name":"Department of Oral Physiology, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan Bi","family":"Ahn","sequence":"additional","affiliation":[{"name":"Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han Byeol","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juping","family":"Xing","sequence":"additional","affiliation":[{"name":"Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joo Won","family":"Yoon","sequence":"additional","affiliation":[{"name":"Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"KABB, Daegu 42212, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong Min","family":"Lee","sequence":"additional","affiliation":[{"name":"KABB, Daegu 42212, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"KABB, Daegu 42212, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Physiology, Yonsei University College of Medicine, Seoul 03722, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1111\/vec.13044","article-title":"Association of Veterinary Hematology and Transfusion Medicine (AVHTM) Transfusion Reaction Small Animal Consensus Statement (TRACS). 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