{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:47:35Z","timestamp":1787161655471,"version":"build-2736575974"},"reference-count":49,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Maryland School of Dentistry"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Root caries caused by cariogenic bacteria are a burden on a large number of individuals worldwide, especially the elderly. Applying a protective coating to exposed root surfaces has the potential to inhibit the development of caries, thus preserving natural teeth. This study aimed to develop a novel antibacterial coating to combat root caries and evaluate its effectiveness using the antibacterial monomer dimethylaminohexadecyl methacrylate (DMAHDM). DMAHDM was synthesized and incorporated into a resin consisting of 55.8% urethane dimethacrylate (UDMA) and 44.2% TEG-DVBE (UV) at a 10% mass fraction of glass filler. Multiple concentrations of DMAHDM were tested for their impact on the resin\u2019s mechanical and physical properties. S. mutans biofilms grown on resin disks were analyzed for antibacterial efficacy. Cytotoxicity was assessed against human gingival fibroblasts (HGFs). The results showed an 8-log reduction in colony-forming units (CFUs) against S. mutans biofilm (mean \u00b1 sd; n = 6) (p &lt; 0.05) when 5% DMAHDM was incorporated into the UV resin. There was a 90% reduction in metabolic activity and lactic acid production. A low level of cytotoxicity against HGF was observed without compromising the physical and mechanical properties of the resin. This coating material demonstrated promising physical properties, potent antibacterial effects, and low toxicity, suggesting its potential to protect exposed roots from caries in various dental procedures and among elderly individuals with gingival recession.<\/jats:p>","DOI":"10.3390\/jfb15060168","type":"journal-article","created":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T08:06:06Z","timestamp":1718784366000},"page":"168","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Novel Resin-Based Antibacterial Root Surface Coating Material to Combat Dental Caries"],"prefix":"10.3390","volume":"15","author":[{"given":"Nader","family":"Almutairi","sequence":"first","affiliation":[{"name":"PhD Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA"},{"name":"Department of Conservative Dental Sciences, College of Dentistry, Prince Sattam bin Abdulaziz University, Alkharj 16245, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7926-2673","authenticated-orcid":false,"given":"Abdullah","family":"Alhussein","sequence":"additional","affiliation":[{"name":"Department of Restorative Dental Science, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Alenizy","sequence":"additional","affiliation":[{"name":"PhD Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA"},{"name":"Department of Restorative Dental Sciences, University of Hail, Hail 55475, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3524-2515","authenticated-orcid":false,"given":"Ibrahim","family":"Ba-Armah","sequence":"additional","affiliation":[{"name":"PhD Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA"},{"name":"Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jirun","family":"Sun","sequence":"additional","affiliation":[{"name":"The ADA Forsyth Institute, Cambridge, MA 02142, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Weir","sequence":"additional","affiliation":[{"name":"Department of Biomaterials and Regenerative Dental Medicine, University of Maryland School of Dentistry, Baltimore, MD 21201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hockin H. K.","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biomaterials and Regenerative Dental Medicine, University of Maryland School of Dentistry, Baltimore, MD 21201, USA"},{"name":"Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA"},{"name":"Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1111\/prd.12338","article-title":"Prevention and Management of Periodontal Diseases and Dental Caries in the Older Adults","volume":"84","author":"Lamster","year":"2020","journal-title":"Periodontol. 2000"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1159\/000526797","article-title":"Development of a Root Caries Prediction Model in a Population of Dental Attenders","volume":"56","author":"Fee","year":"2022","journal-title":"Caries Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Su, W.S., and Chang, C.Y. (2024). Root Caries in Older Adults: A Co-Citation Network Analysis (1980\u20132023). Gerodontology.","DOI":"10.1111\/ger.12745"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.adaj.2021.09.004","article-title":"Update on the Prevalence of Untreated Caries in the US Adult Population, 2017\u20132020","volume":"153","year":"2022","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/ger.12511","article-title":"The Burden of Root Caries: Updated Perspectives and Advances on Management Strategies","volume":"38","author":"AlQranei","year":"2021","journal-title":"Gerodontology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1111\/adj.12550","article-title":"Status and Progress of Treatment Methods for Root Caries in the Last Decade: A Literature Review","volume":"63","author":"Cai","year":"2018","journal-title":"Aust. Dent. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"711","DOI":"10.2741\/e281","article-title":"Dentin: Structure, Composition and Mineralization: The Role of Dentin ECM in Dentin Formation and Mineralization","volume":"3","author":"Goldberg","year":"2011","journal-title":"Front. Biosci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4743","DOI":"10.2147\/IJN.S107624","article-title":"Demineralisation-Remineralisation-Dynamics-in-Teeth-and-Bone","volume":"11","author":"Neel","year":"2016","journal-title":"Int. J. Nanomed. Dovepress"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1600-0757.1997.tb00095.x","article-title":"Dental Cementum: The Dynamic Tissue Covering of the Root","volume":"13","author":"Bosshardt","year":"1997","journal-title":"Periodontol. 2000"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sarna-Bo\u015b, K., Boguta, P., Skic, K., Wi\u0105cek, D., Maksymiuk, P., Sobieszcza\u0144ski, J., and Cha\u0142as, R. (2022). Physicochemical Properties and Surface Characteristics of Ground Human Teeth. Molecules, 27.","DOI":"10.3390\/molecules27185852"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/0003-9969(87)90085-9","article-title":"The Mineral Solubility of Human Tooth Roots","volume":"32","author":"Hoppenbrouwers","year":"1987","journal-title":"Archs Oral Bid."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1159\/000479303","article-title":"Specificities of Caries on Root Surface","volume":"26","author":"Parolo","year":"2017","journal-title":"Monogr. Oral. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/j.1754-4505.2012.00318.x","article-title":"Prevention of Root Caries: A Literature Review of Primary and Secondary Preventive Agents","volume":"33","author":"Gluzman","year":"2013","journal-title":"Spec. Care Dent."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1177\/00220345231189992","article-title":"A Remineralizing and Antibacterial Coating for Arresting Caries","volume":"102","author":"Lu","year":"2023","journal-title":"J. Dent. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.dental.2017.10.007","article-title":"High Performance Dental Resin Composites with Hydrolytically Stable Monomers","volume":"34","author":"Wang","year":"2018","journal-title":"Dent. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1016\/j.dental.2022.08.005","article-title":"Novel Antibacterial Low-Shrinkage-Stress Resin-Based Cement","volume":"38","author":"AlSahafi","year":"2022","journal-title":"Dent. Mater."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Mitwalli, H., Balhaddad, A.A., AlSahafi, R., Oates, T.W., Melo, M.A.S., Xu, H.H.K., and Weir, M.D. (2020). Novel CaF2 nanocomposites with Antibacterial Function and Fluoride and Calcium Ion Release to Inhibit Oral Biofilm and Protect Teeth. J. Funct. Biomater., 11.","DOI":"10.3390\/jfb11030056"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.msec.2016.12.084","article-title":"Physical, Chemical and Antimicrobial Evaluation of a Composite Material Containing Quaternary Ammonium Salt for Braces Cementation","volume":"73","author":"Sugii","year":"2017","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Liang, X., Yu, B., Ye, L., Lin, D., Zhang, W., Zhong, H.-J., He, J., Ortega, V., Liang, X., and Yu, B. (2024). Recent Advances in Quaternary Ammonium Monomers for Dental Applications. Materials, 17.","DOI":"10.3390\/ma17020345"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.dental.2011.10.004","article-title":"Synthesis and Characterization of Dimethacrylates Containing Quaternary Ammonium Functionalities for Dental Applications","volume":"28","author":"Antonucci","year":"2012","journal-title":"Dent. Mater."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1021\/acs.jchemed.8b00659","article-title":"Analysis of Double Bond Conversion of Photopolymerizable Monomers by FTIR-ATR Spectroscopy","volume":"96","year":"2019","journal-title":"J. Chem. Educ."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1021\/acsbiomaterials.3c01786","article-title":"Mechanical and Antimicrobial Properties of Boron Nitride\/Methacrylic Acid Quaternary Ammonium Composites Reinforced Dental Flowable Resins","volume":"10","author":"Wang","year":"2024","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_23","unstructured":"(2012). Dentistry: Root Canal Sealing Materials. Standard No. ISO 6876:2012."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/j.jds.2021.09.034","article-title":"Physicochemical Properties of a Novel Bioceramic Silicone-Based Root Canal Sealer","volume":"17","author":"Lyu","year":"2022","journal-title":"J. Dent. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1080\/09205063.2021.1878805","article-title":"Long-Term Antibacterial Activity and Cytocompatibility of Novel Low-Shrinkage-Stress, Remineralizing Composites","volume":"32","author":"Bhadila","year":"2021","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0300-5712(97)00013-4","article-title":"Antibacterial Activity of Dentine Primer Containing MDPB after Curing","volume":"26","author":"Imazato","year":"1998","journal-title":"J. Dent."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"102890","DOI":"10.1016\/j.ijadhadh.2021.102890","article-title":"Application of Silver Nanoparticles in Situ Synthesized in Dental Adhesive Resin","volume":"108","author":"Yang","year":"2021","journal-title":"Int. J. Adhes. Adhes."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"7251","DOI":"10.1007\/s00253-022-12211-7","article-title":"Candida Albicans CHK1 Gene Regulates Its Cross-Kingdom Interactions with Streptococcus Mutans to Promote Caries","volume":"106","author":"Liu","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Zhang, L., Shen, Y., Qiu, L., Yu, F., Hu, X., Wang, M., Sun, Y., Pan, Y., and Zhang, K. (2022). The Suppression Effect of SCH-79797 on Streptococcus Mutans Biofilm Formation. J. Oral. Microbiol., 14.","DOI":"10.1080\/20002297.2022.2061113"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Zhang, K., Xiang, Y., Peng, Y., Tang, F., Cao, Y., Xing, Z., Li, Y., Liao, X., Sun, Y., and He, Y. (2022). Influence of Fluoride-Resistant Streptococcus Mutans Within Antagonistic Dual-Species Biofilms Under Fluoride In Vitro. Front. Cell Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.801569"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Hu, X., Wang, M., Shen, Y., Zhang, L., Pan, Y., Sun, Y., and Zhang, K. (2022). Regulatory Effect of Irresistin-16 on Competitive Dual-Species Biofilms Composed of Streptococcus Mutans and Streptococcus Sanguinis. Pathogens, 11.","DOI":"10.3390\/pathogens11010070"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/j.adaj.2018.06.004","article-title":"Original Contributions Variation in Composite Degree of Conversion and Microflexural Strength for Different Curing Lights and Surface Locations","volume":"149","author":"Eshmawi","year":"2018","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1002\/jbm.b.33212","article-title":"Wear of Nanofilled Dental Composites at Varying Filler Concentrations","volume":"103","author":"Lawson","year":"2015","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1177\/0022034510379602","article-title":"The Role of Bacteria in the Caries Process: Ecological Perspectives","volume":"90","author":"Takahashi","year":"2011","journal-title":"J. Dent. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e428","DOI":"10.1016\/j.dental.2017.09.012","article-title":"ScienceDirect Flowable Composites for Restoration of Non-Carious Cervical Lesions: Results after Five Years","volume":"33","author":"Cieplik","year":"2017","journal-title":"Dent. Mater."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1007\/s00784-018-2509-8","article-title":"One-Year Clinical Evaluation of Bulk-Fill Flowable vs. Regular Nanofilled Composite in Non-Carious Cervical Lesions","volume":"23","author":"Canali","year":"2019","journal-title":"Clin. Oral. Investig."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Battancs, E., Fr\u00e1ter, M., S\u00e1ry, T., G\u00e1l, E., Braunitzer, G., Bal\u00e1zs, S.P., and Garoushi, S. (2021). Fracture Behavior and Integrity of Different Direct Restorative Materials to Restore Noncarious Cervical Lesions. Polymers, 13.","DOI":"10.3390\/polym13234170"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.actbio.2020.07.057","article-title":"Bioactive Low-Shrinkage-Stress Nanocomposite Suppresses S. Mutans Biofilm and Preserves Tooth Dentin Hardness","volume":"114","author":"Bhadila","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Alhussein, A., Alsahafi, R., Wang, X., Mitwalli, H., Filemban, H., Hack, G.D., Oates, T.W., Sun, J., Weir, M.D., and Xu, H.H.K. (2023). Novel Dental Low-Shrinkage-Stress Composite with Antibacterial Dimethylaminododecyl Methacrylate Monomer. J. Funct. Biomater., 14.","DOI":"10.3390\/jfb14070335"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/j.dental.2020.05.010","article-title":"Novel Pit and Fissure Sealant Containing Nano-CaF2 and Dimethylaminohexadecyl Methacrylate with Double Benefits of Fluoride Release and Antibacterial Function","volume":"36","author":"Fei","year":"2020","journal-title":"Dent. Mater."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0109-5641(00)00062-2","article-title":"Determination of Double Bond Conversion in Dental Resins by near Infrared Spectroscopy","volume":"17","author":"Stansbury","year":"2001","journal-title":"Dent. Mater."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.jdent.2018.12.001","article-title":"Novel Multifunctional Nanocomposite for Root Caries Restorations to Inhibit Periodontitis-Related Pathogens","volume":"81","author":"Xiao","year":"2019","journal-title":"J. Dent."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1016\/j.dental.2019.07.014","article-title":"Novel Root Canal Sealer with Dimethylaminohexadecyl Methacrylate, Nano-Silver and Nano-Calcium Phosphate to Kill Bacteria inside Root Dentin and Increase Dentin Hardness","volume":"35","author":"Baras","year":"2019","journal-title":"Dent. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5509","DOI":"10.1038\/s41598-018-23831-3","article-title":"Drug Resistance of Oral Bacteria to New Antibacterial Dental Monomer Dimethylaminohexadecyl Methacrylate","volume":"8","author":"Wang","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3637","DOI":"10.1007\/s00784-021-04334-4","article-title":"Evaluation of the Ability of Adhesives with Antibacterial and Remineralization Functions to Prevent Secondary Caries in Vivo","volume":"26","author":"Liu","year":"2022","journal-title":"Clin. Oral. Investig."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ibrahim, M.S., Ibrahim, A.S., Balhaddad, A.A., Weir, M.D., Lin, N.J., Tay, F.R., Oates, T.W., Xu, H.H.K., and Melo, M.A.S. (2019). A Novel Dental Sealant Containing Dimethylaminohexadecyl Methacrylate Suppresses the Cariogenic Pathogenicity of Streptococcus Mutans Biofilms. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20143491"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Alsahafi, R., Balhaddad, A.A., Mitwalli, H., Ibrahim, M.S., Melo, M.A.S., Oates, T.W., Xu, H.H.K., and Weir, M.D. (2020). Novel Crown Cement Containing Antibacterial Monomer and Calcium Phosphate Nanoparticles. Nanomaterials, 10.","DOI":"10.3390\/nano10102001"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1067\/mpr.2001.113778","article-title":"A Review of Saliva: Normal Composition, Flow, and Function","volume":"85","author":"Humphrey","year":"2001","journal-title":"J. Prosthet. Dent."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/j.mseb.2011.04.008","article-title":"Improved Cytotoxicity Testing of Magnesium Materials","volume":"176","author":"Fischer","year":"2011","journal-title":"Mater. Sci. Eng. 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