{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T08:24:00Z","timestamp":1768638240753,"version":"3.49.0"},"reference-count":49,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T00:00:00Z","timestamp":1728777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Slovenian Research and Innovation Agency","award":["P2-0424"],"award-info":[{"award-number":["P2-0424"]}]},{"name":"Slovenian Research and Innovation Agency","award":["BI-RS 18\/19-043"],"award-info":[{"award-number":["BI-RS 18\/19-043"]}]},{"name":"Slovenian Research and Innovation Agency","award":["451-03-66\/2024-03\/200017"],"award-info":[{"award-number":["451-03-66\/2024-03\/200017"]}]},{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["P2-0424"],"award-info":[{"award-number":["P2-0424"]}]},{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["BI-RS 18\/19-043"],"award-info":[{"award-number":["BI-RS 18\/19-043"]}]},{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["451-03-66\/2024-03\/200017"],"award-info":[{"award-number":["451-03-66\/2024-03\/200017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Silver nanoparticles (Ag NPs) conjugated with amino-functionalized cellulose nanofibrils (NH2\u2212CNFs) were in situ-prepared by reducing silver ions with free amino groups from NH2\u2212CNFs. The spectroscopy and transmission electron microscopy measurements confirmed the presence of non-agglomerated nanometer-in-size Ag NPs within micrometer-large NH2\u2212CNFs of high (20 wt.-%) content. Although the consumption of amino groups during the formation of Ag NPs lowers the \u03b6-potential and surface charge of prepared inorganic\u2013organic hybrids (from +31.3 to +19.9 mV and from 2.4 to 1.0 mmol\/g at pH 7, respectively), their values are sufficiently positive to ensure electrostatic interaction with negatively charged cell walls of pathogens in acidic and slightly (up to pH ~8.5) alkaline solutions. The antimicrobial activity of hybrid microparticles against various pathogens (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans) is comparable with pristine NH2\u2212CNFs. However, a long-timescale use of hybrids ensures the slow and controlled release of Ag+ ions to surrounding media (less than 1.0 wt.-% for one month).<\/jats:p>","DOI":"10.3390\/jfb15100304","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T06:46:33Z","timestamp":1728888393000},"page":"304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antimicrobial Activity of Amino-Modified Cellulose Nanofibrils Decorated with Silver Nanoparticles"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6440-6577","authenticated-orcid":false,"given":"Vesna","family":"Lazi\u0107","sequence":"first","affiliation":[{"name":"Vin\u010da Institute of Nuclear Sciences\u2014National Institute of the Republic of Serbia, University of Belgrade, Centre of Excellence for Photoconversion, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jovan M.","family":"Nedeljkovi\u0107","sequence":"additional","affiliation":[{"name":"Vin\u010da Institute of Nuclear Sciences\u2014National Institute of the Republic of Serbia, University of Belgrade, Centre of Excellence for Photoconversion, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8521-0941","authenticated-orcid":false,"given":"Vanja","family":"Kokol","sequence":"additional","affiliation":[{"name":"Faculty of Mechanical Engineering, University of Maribor, 2000 Maribor, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Trache, D., Tarchoun, A.F., Derradji, M., Hamidon, T.S., Masruchin, N., Brosse, N., and Hussin, M.H. (2020). Nanocellulose: From Fundamentals to Advanced Applications. Front. Chem., 8.","DOI":"10.3389\/fchem.2020.00392"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"11575","DOI":"10.1021\/acs.chemrev.7b00627","article-title":"Nanocellulose, a versatile green platform: From biosources to materials and their applications","volume":"118","author":"Thomas","year":"2018","journal-title":"Chem. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Thomas, P., Duolikun, T., Rumjit, N.P., Moosavi, S., Lai, C.W., Johan, M.R.B., and Fen, L.B. (2020). Comprehensive review on nanocellulose: Recent developments, challenges and future prospects. J. Mech. Behav. Biomed., 110.","DOI":"10.1016\/j.jmbbm.2020.103884"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5429","DOI":"10.1007\/s10570-022-04619-1","article-title":"Dialdehyde cellulose as a niche material for versatile applications: An overview","volume":"29","author":"Dalei","year":"2022","journal-title":"Cellulose"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1872","DOI":"10.1039\/D1MA00049G","article-title":"Recent advances in nanocellulose processing, functionalization and applications: A review","volume":"2","author":"Thakur","year":"2021","journal-title":"Mater. Adv."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.carbpol.2009.05.015","article-title":"The influence of silver content on antimicrobial activity and color of cotton fabrics functionalized with Ag nanoparticles","volume":"78","author":"Vodnik","year":"2009","journal-title":"Carbohydr. Polym."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2897","DOI":"10.1021\/am3007609","article-title":"Preparation of copper nanoparticles coated cellulose films with antibacterial properties through one-step reduction","volume":"4","author":"Jia","year":"2012","journal-title":"ACS Appl. Mater. Inter."},{"key":"ref_8","first-page":"2150","article-title":"Review of silver nanoparticles (AgNPs)-cellulose antibacterial composites","volume":"13","author":"Xu","year":"2018","journal-title":"BioResources"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1007\/s10570-011-9510-6","article-title":"Functionalization of cotton fabrics with corona\/air RF plasma and colloidal TiO2 nanoparticles","volume":"18","author":"Baily","year":"2011","journal-title":"Cellulose"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2482","DOI":"10.1021\/acs.biomac.5b00758","article-title":"Synthesis, characterization, and antimicrobial efficacy of photomicrobicidal cellulose paper","volume":"16","author":"Carpenter","year":"2015","journal-title":"Biomacromolecules"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.cej.2014.09.096","article-title":"Simple method of deposition of CuO nanoparticles on a cellulose paper and its antibacterial activity","volume":"262","author":"Booshehri","year":"2015","journal-title":"Chem. Eng. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.carbpol.2017.02.020","article-title":"Extraction of nanocellulose and in-situ casting of ZnO\/cellulose nanocomposite with enhanced photocatalytic and antibacterial activity","volume":"164","author":"Lefatshe","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1515\/chem-2018-0006","article-title":"The preparation and antibacterial activity of cellulose\/ZnO composite: A review","volume":"16","author":"Zhao","year":"2018","journal-title":"Open Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1007\/s10570-019-02889-w","article-title":"Cellulose nanomaterials: New generation materials for solving global issues","volume":"27","author":"Mokhena","year":"2020","journal-title":"Cellulose"},{"key":"ref_15","first-page":"1105","article-title":"Preparation of gentamicin conjugated cellulose nanocrystals and evaluation of efficacy on different microorganisms","volume":"27","year":"2021","journal-title":"Eur. J. Sci. Tech."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"100512","DOI":"10.1016\/j.cogsc.2021.100512","article-title":"Nanocellulose-based composites for packaging applications","volume":"31","author":"Amara","year":"2021","journal-title":"Curr. Opin. Green Sust."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"112126","DOI":"10.1016\/j.indcrop.2020.112126","article-title":"Preparation and antibacterial activity of a cellulose-based schiff base derived from dialdehyde cellulose and L-lysine","volume":"145","author":"Zhang","year":"2020","journal-title":"Ind. Crop Prod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1007\/s10570-021-03692-2","article-title":"Preparation of,3-dialdehyde microcrystalline cellulose particles crosslinked with \u03b5-poly-L-lysine and their antibacterial activity","volume":"28","author":"He","year":"2021","journal-title":"Cellulose"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1007\/s00253-010-2920-9","article-title":"Antimicrobial polymers: Mechanism of action, factors of activity, and applications","volume":"89","author":"Timofeeva","year":"2011","journal-title":"Appl. Microbiol. Biot."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1080\/17435390.2016.1196251","article-title":"Multilaboratory evaluation of 15 bioassays for (eco) toxicity screening and hazard ranking of engineered nanomaterials: FP7 project NANOVALID","volume":"10","author":"Bondarenko","year":"2016","journal-title":"Nanotoxicology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s40820-020-0408-4","article-title":"A review on surface-functionalized cellulosic nanostructures as biocompatible antibacterial materials","volume":"12","author":"Tavakolian","year":"2020","journal-title":"Nano-Micro Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"5296","DOI":"10.1021\/la700553d","article-title":"Silver nanoparticles capped by oleylamine: Formation, growth, and self-organization","volume":"23","author":"Chen","year":"2007","journal-title":"Langmuir"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1021\/cm4000476","article-title":"Oleylamine in Nanoparticle Synthesis","volume":"25","author":"Mourdikoudis","year":"2013","journal-title":"Chem. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6838","DOI":"10.1007\/s10853-014-8386-x","article-title":"Synthesis, characterization, and antimicrobial activity of poly(GMA-co-EGDMA) polymer decorated with silver nanoparticles","volume":"49","author":"Vukoje","year":"2014","journal-title":"J. Mater. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1002\/pc.23684","article-title":"Synthesis, characterization, and antimicrobial activity of silver nanoparticles on poly(GMA-co-EGDMA) polymer support","volume":"38","author":"Vukoje","year":"2017","journal-title":"Polym. Compos."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2901","DOI":"10.1002\/pc.25120","article-title":"Size-Dependent Antibacterial Properties of Ag Nanoparticles Supported by Amino-Functionalized Poly(GMA-co-EGDMA) Polymer","volume":"40","author":"Vukoje","year":"2019","journal-title":"Polym. Compos."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.vacuum.2017.10.039","article-title":"Antibacterial ability of supported silver nanoparticles by functionalized hydroxyapatite with 5-aminosalicylic acid","volume":"148","author":"Ahrenkiel","year":"2018","journal-title":"Vacuum"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4018","DOI":"10.1002\/slct.201900628","article-title":"Antimicrobial activity of silver nanoparticles supported by magnetite","volume":"4","author":"Mihajlovski","year":"2019","journal-title":"ChemistrySelect"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"100575","DOI":"10.1016\/j.fpsl.2020.100575","article-title":"Dextran-coated silver nanoparticles for improved barrier and controlled antimicrobial properties of nanocellulose films used in food packaging","volume":"26","author":"Vivod","year":"2020","journal-title":"Food Packag. Shelf"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3589","DOI":"10.1007\/s10570-024-05847-3","article-title":"Slot-die coating of cellulose nanocrystals and chitosan for improved barrier properties of paper","volume":"31","author":"Ruberto","year":"2024","journal-title":"Cellulose"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6545","DOI":"10.1007\/s10570-021-03946-z","article-title":"Antimicrobial properties of viscose yarns ring-spun with integrated amino-functionalized nanocellulose","volume":"28","author":"Kokol","year":"2021","journal-title":"Cellulose"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"120603","DOI":"10.1016\/j.carbpol.2023.120603","article-title":"Antibacterial and degradation properties of dialdehyded and aminohexamethylated nanocelluloses","volume":"311","author":"Kokol","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Xu, Q., Huang, X., Guo, L., Wang, Y., and Jin, L. (2021). Enhancing Removal of Cr(VI), Pb2+, and Cu2+ from Aqueous Solutions Using Amino-Functionalized Cellulose Nanocrystal. Molecules, 26.","DOI":"10.3390\/molecules26237315"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7805","DOI":"10.1007\/s10570-023-05339-w","article-title":"Antibacterial properties of functionalized cellulose extracted from deproteinized soybean hulls","volume":"30","author":"Tummino","year":"2023","journal-title":"Cellulose"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Park, S., Baker, J.O., Himmel, M.E., Parilla, P.A., and Johnson, D.K. (2010). Cellulose crystallinity index: Measurement techniques and their impact on interpreting cellulase performance. Biotechnol. Biofuels, 3.","DOI":"10.1186\/1754-6834-3-10"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3899","DOI":"10.1007\/s10570-018-1871-7","article-title":"Morphology of the nanocellulose produced by periodate oxidation and reductive treatment of cellulose fibers","volume":"25","author":"Errokh","year":"2018","journal-title":"Cellulose"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/0301-4622(95)00004-H","article-title":"Difference in surface properties between Escherichia coli and Staphylococcus aureus as revealed by electrophoretic mobility measurements","volume":"55","author":"Sonohara","year":"1995","journal-title":"Biophys. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.ijggc.2016.11.023","article-title":"Kinetics of CO2 absorption in aqueous hexamethylenediamine","volume":"56","author":"Mondal","year":"2017","journal-title":"Int. J. Greenh. Gas Control"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Rashki, S., Shakour, N., Yousefi, Z., Rezaei, M., Homayoonfal, M., Khabazian, E., Atyabi, F., Aslanbeigi, F., Lapavandani, R.S., and Mazaheri, S. (2021). Cellulose-Based Nanofibril Composite Materials as a New Approach to Fight Bacterial Infections. Front. Bioeng. Biotech., 9.","DOI":"10.3389\/fbioe.2021.732461"},{"key":"ref_40","first-page":"1","article-title":"Envelope structures of gram-positive bacteria","volume":"404","author":"Rajagopal","year":"2017","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"16248","DOI":"10.1021\/jp063826h","article-title":"Silver Colloid Nanoparticles: Synthesis, Characterization, and Their Antibacterial Activity","volume":"110","author":"Prucek","year":"2006","journal-title":"J. Phys. Chem. B"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1002\/1097-4636(20001215)52:4<662::AID-JBM10>3.0.CO;2-3","article-title":"A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus","volume":"15","author":"Feng","year":"2000","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3974","DOI":"10.1039\/C3RA44507K","article-title":"Size-controlled silver nanoparticles synthesized over the range 5\u2013100 nm using the same protocol and their antibacterial Efficacy","volume":"4","author":"Agnihotri","year":"2014","journal-title":"RSC Adv."},{"key":"ref_44","first-page":"105","article-title":"The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus","volume":"7","author":"Parvekar","year":"2020","journal-title":"Biomater. Investig. Dent."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s12030-009-9029-1","article-title":"Silver Nanoparticles Toxicity and Bactericidal Effect against Methicillin-Resistant Staphylococcus aureus: Nanoscale Does Matter","volume":"5","author":"Villegas","year":"2009","journal-title":"NanoBiotechnology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"7328","DOI":"10.1039\/c3nr00024a","article-title":"Immobilized silver nanoparticles enhance contact killing and show highest efficacy: Elucidation of the mechanism of bactericidal action of silver","volume":"5","author":"Agnihotri","year":"2013","journal-title":"Nanoscale"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1002\/adma.201001934","article-title":"Long-Term Antimicrobial Effect of Silicon Nanowires Decorated with Silver Nanoparticles","volume":"22","author":"Lv","year":"2010","journal-title":"Adv. Mater."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Kokol, V., Kos, M., Vivod, V., and Gunde-Cimerman, N. (2023). Cationised Fibre-Based Cellulose Multi-Layer Membranes for Sterile and High-Flow Bacteria Retention and Inactivation. Membranes, 13.","DOI":"10.3390\/membranes13030284"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6333","DOI":"10.1016\/j.biomaterials.2009.07.065","article-title":"Antifungal activity of silver nanoparticles against Candida spp","volume":"30","author":"Prucek","year":"2009","journal-title":"Biomaterials"}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/15\/10\/304\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:12:29Z","timestamp":1760112749000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/15\/10\/304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,13]]},"references-count":49,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["jfb15100304"],"URL":"https:\/\/doi.org\/10.3390\/jfb15100304","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,13]]}}}