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Comminuted fractures that exceed the critical size may heal poorly due to deficient or interrupted vascularization, resulting in an insufficient number of progenitor cells necessary for bone regeneration. In this context, 3D printing techniques using poly-L-lactic acid\/graphene oxide (PLLA\/GO) aim to address this issue by creating customized scaffolds combined with canine placenta hydrogel and mesenchymal stem cells for use in goat mandibles, compared to a control group using titanium plate fixation. Ten canine placentas were decellularized and characterized using histological techniques. A hydrogel derived from the canine placenta extracellular matrix (cpECM) was produced to improve cell attachment to the scaffolds. In vitro cytotoxicity and cell adhesion to the cpECM hydrogel were assessed by scanning electron microscopy (SEM). The resulting biomaterials, cpECM hydrogel and PLLA\/GO scaffolds, maintained their functional structure and supported cell adhesion, maintenance, and proliferation in vitro. Thermography showed that PLLA\/GO scaffolds with cpECM hydrogel performed effectively, similar to the control group. Computed tomography scans revealed bone calluses, suggesting an ongoing repair process. These findings demonstrate the innovative technological potential of these materials for use in surgical interventions. Future studies on PLLA\/GO scaffolds will provide further insights into their effects on goat models.<\/jats:p>","DOI":"10.3390\/jfb15100311","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T08:53:11Z","timestamp":1729500791000},"page":"311","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["PLLA\/GO Scaffolds Filled with Canine Placenta Hydrogel and Mesenchymal Stem Cells for Bone Repair in Goat Mandibles"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7848-8558","authenticated-orcid":false,"given":"Thamires","family":"Santos-Silva","sequence":"first","affiliation":[{"name":"Department of Surgery, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e1cio Silva","family":"Viana","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal Campus 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0199-6105","authenticated-orcid":false,"given":"Andrea Barros Piazzon S.","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Department of Animal Morphology and Physiology, School of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal Campus 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabr\u00edcio Singaretti","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Department of Animal Morphology and Physiology, School of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal Campus 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca de Oliveira","family":"Horvath-Pereira","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2628-8746","authenticated-orcid":false,"given":"Leandro Norberto","family":"da Silva-J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"},{"name":"Department of Animal Anatomy, University of Marilia, Mirante, Mar\u00edlia 17525-902, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1709-0984","authenticated-orcid":false,"given":"Michelle Silva","family":"Araujo","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4354-6487","authenticated-orcid":false,"given":"Paulo Alescio","family":"Canola","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal Campus 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu\u00eds Gustavo Gosuen G.","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal Campus 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7143-2255","authenticated-orcid":false,"given":"Marcelo Melo","family":"Soares","sequence":"additional","affiliation":[{"name":"Institute of Orofacial Osteogenesis Rehabilitation S\/S Ltda., Vila Ol\u00edmpia 04532-060, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4979-115X","authenticated-orcid":false,"given":"Maria Angelica","family":"Miglino","sequence":"additional","affiliation":[{"name":"Department of Animal Anatomy, University of Marilia, Mirante, Mar\u00edlia 17525-902, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1016\/j.jcms.2015.06.041","article-title":"Regenerative medicine in the treatment of alveolar cleft defect: A systematic review of the literature","volume":"43","author":"Khojasteh","year":"2015","journal-title":"J. Cranio-Maxillofac. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.bone.2016.02.020","article-title":"Inflammation, fracture and bone repair","volume":"86","author":"Loi","year":"2016","journal-title":"Bone"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s12663-017-1043-6","article-title":"Efficacy of Titanium Mesh Osteosynthesis in Maxillofacial Fractures","volume":"17","author":"Kaur","year":"2018","journal-title":"J. Maxillofac. Oral Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.jormas.2020.10.003","article-title":"Usage of stem cells in oral and maxillofacial region","volume":"122","author":"Pourlak","year":"2021","journal-title":"J. Stomatol. Oral Maxillofac. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1016\/j.bjps.2016.05.003","article-title":"A systematic review of functional outcome and quality of life following reconstruction of maxillofacial defects using vascularized free fibula flaps and dental rehabilitation reveals poor data quality","volume":"69","author":"Wijbenga","year":"2016","journal-title":"J. Plast. Reconstr. Aesthetic Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S193","DOI":"10.1038\/550S193a","article-title":"Non-union bone fracture: A quicker fix","volume":"550","author":"Holmes","year":"2017","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.biomaterials.2018.07.017","article-title":"Bone regeneration strategies: Engineered scaffolds, bioactive molecules and stem cells Current stage and future perspectives","volume":"180","author":"Bolander","year":"2018","journal-title":"Biomaterials"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1111\/jcmm.17096","article-title":"Biological and molecular profile of fracture non-union tissue: A systematic review and an update on current insights","volume":"26","author":"Panteli","year":"2022","journal-title":"J. Cell. Mol. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1016\/j.injury.2011.03.035","article-title":"Angiogenesis in Bone Regeneration","volume":"42","author":"Hankenson","year":"2011","journal-title":"Injury"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"100","DOI":"10.22203\/eCM.v015a08","article-title":"Osteogenesis and angiogenesis: The potential for engineering bone","volume":"15","author":"Kanczler","year":"2008","journal-title":"Eur. Cells Mater."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.bone.2015.11.011","article-title":"Tissue engineering strategies for promoting vascularized bone regeneration","volume":"83","author":"Almubarak","year":"2016","journal-title":"Bone"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Grosso, A., Burger, M.G., Lunger, A., Schaefer, D.J., Banfi, A., and Di Maggio, N. (2017). It takes two to tango: Coupling of angiogenesis and osteogenesis for bone regeneration. Front. Bioeng. Biotechnol., 5.","DOI":"10.3389\/fbioe.2017.00068"},{"key":"ref_13","first-page":"224","article-title":"Bone grafts and biomaterials substitutes for bone defect repair: A review","volume":"2","author":"Wang","year":"2017","journal-title":"Bioact. Mater."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Alonzo, M., Alvarez Primo, F., Anil Kumar, S., Mudloff, J.A., Dominguez, E., Fregoso, G., Ortiz, N., Weiss, W.M., and Joddar, B. (2021). Bone tissue engineering techniques, advances, and scaffolds for treatment of bone defects. Curr. Opin. Biomed. Eng., 17.","DOI":"10.1016\/j.cobme.2020.100248"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1111\/prd.12414","article-title":"Complications in bone-grafting procedures: Classification and management","volume":"88","author":"Molina","year":"2022","journal-title":"Periodontology 2000"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"25","DOI":"10.14302\/issn.2576-6694.jbbs-18-2143","article-title":"Calcium Orthophosphate (CaPO4) Scaffolds for Bone Tissue Engineering Applications","volume":"1","author":"Dorozhkin","year":"2018","journal-title":"J. Biotechnol. Biomed. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Kargozar, S., Milan, P.B., Baino, F., and Mozafari, M. (2019). Nanoengineered biomaterials for bone\/dental regeneration. Nanoengineered Biomaterials for Regenerative Medicine, Elsevier.","DOI":"10.1016\/B978-0-12-813355-2.00002-8"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Rastogi, P., and Kandasubramanian, B. (2019). Review of alginate-based hydrogel bioprinting for application in tissue engineering. Biofabrication, 11.","DOI":"10.1088\/1758-5090\/ab331e"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Rasyida, A., Halimah, S., Wijayanti, I.D., Wicaksono, S.T., Nurdiansah, H., Silaen, Y.M.T., Ni\u2019mah, Y.L., Ardhyananta, H., and Purniawan, A. (2023). A Composite of Hydrogel Alginate\/PVA\/r-GO for Scaffold Applications with Enhanced Degradation and Biocompatibility Properties. Polymers, 15.","DOI":"10.3390\/polym15030534"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Jun, I., Han, H.S., Edwards, J.R., and Jeon, H. (2018). Electrospun Fibrous Scaffolds for Tissue Engineering: Viewpoints on Architecture and Fabrication. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19030745"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Wang, C., Fu, L., Ye, S., Wang, M., and Zhou, Y. (2019). Fabrication and Application of Novel Porous Scaffold in Situ-Loaded Graphene Oxide and Osteogenic Peptide by Cryogenic 3D Printing for Repairing Critical-Sized Bone Defect. Molecules, 24.","DOI":"10.3390\/molecules24091669"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"110595","DOI":"10.1016\/j.msec.2019.110595","article-title":"Development of new biocompatible 3D printed graphene oxide-based scaffolds","volume":"110","author":"Belaid","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"da Silva, T.S., Horvath-Pereira, B.d.O., da Silva-J\u00fanior, L.N., Ten\u00f3rio Fireman, J.V.B., Mattar, M., F\u00e9lix, M., Buchaim, R.L., Carreira, A.C.O., Miglino, M.A., and Soares, M.M. (2023). Three-Dimensional Printing of Graphene Oxide\/Poly-L-Lactic Acid Scaffolds Using Fischer\u2013Koch Modeling. Polymers, 15.","DOI":"10.3390\/polym15214213"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Santos Silva, T., Melo Soares, M., Oliveira Carreira, A.C., de S\u00e1 Schiavo Matias, G., Coming Tegon, C., Massi, M., de Aguiar Oliveira, A., da Silva J\u00fanior, L.N., Costa de Carvalho, H.J., and Don\u00e1 Rodrigues Almeida, G.H. (2021). Biological Characterization of Polymeric Matrix and Graphene Oxide Biocomposites Filaments for Biomedical Implant Applications: A Preliminary Report. Polymers, 13.","DOI":"10.3390\/polym13193382"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100422","DOI":"10.1016\/j.mtchem.2021.100422","article-title":"Synthesis and applications of graphene oxide aerogels in bone tissue regeneration: A review","volume":"20","author":"Berrio","year":"2021","journal-title":"Mater. Today Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2849","DOI":"10.1021\/acsbiomaterials.2c00396","article-title":"Application of Graphene Oxide-Based Hydrogels in Bone Tissue Engineering","volume":"8","author":"Zhihui","year":"2022","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_27","first-page":"963","article-title":"Advanced fabrication for electrospun three-dimensional nanofiber aerogels and scaffolds","volume":"5","author":"Chen","year":"2020","journal-title":"Bioact. Mater."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1515\/ntrev-2020-0093","article-title":"Degradation modeling of poly-L-lactide acid (PLLA) bioresorbable vascular scaffold within a coronary artery","volume":"9","author":"Lin","year":"2020","journal-title":"Nanotechnol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Melo, P., Naseem, R., Corvaglia, I., Montalbano, G., Pontremoli, C., Azevedo, A., Quadros, P., Gentile, P., Ferreira, A.M., and Dalgarno, K. (2020). Processing of Sr2+ Containing Poly L-Lactic Acid-Based Hybrid Composites for Additive Manufacturing of Bone Scaffolds. Front. Mater., 7.","DOI":"10.3389\/fmats.2020.601645"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1097\/00003086-199907000-00030","article-title":"Critical size defect in the goat\u2019s os ilium. A model to evaluate bone grafts and substitutes","volume":"364","author":"Anderson","year":"1999","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"9452","DOI":"10.1016\/j.biomaterials.2010.08.036","article-title":"Osteogenesis and angiogenesis of tissue-engineered bone constructed by prevascularized \u03b2-tricalcium phosphate scaffold and mesenchymal stem cells","volume":"31","author":"Wang","year":"2010","journal-title":"Biomaterials"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2367","DOI":"10.1007\/s10856-007-3348-3","article-title":"Repair of goat tibial defects with bone marrow stromal cells and \u03b2-tricalcium phosphate","volume":"19","author":"Liu","year":"2008","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1525-1594.2010.01042.x","article-title":"Repair of Bone Defect in Caprine Tibia Using a Laminated Scaffold With Bone Marrow Stromal Cells Loaded Poly (L-Lactic Acid)\/\u03b2-Tricalcium Phosphate","volume":"35","author":"Huang","year":"2011","journal-title":"Artif. Organs"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"104497","DOI":"10.1016\/j.jprot.2022.104497","article-title":"Proteomic profile of extracellular matrix from native and decellularized chorionic canine placenta","volume":"256","author":"Carreira","year":"2022","journal-title":"J. Proteomics"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1021\/ja01539a017","article-title":"Preparation of Graphitic Oxide","volume":"80","author":"Hummers","year":"1958","journal-title":"J. Am. Chem. Soc."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1053\/j.ro.2017.04.006","article-title":"Computed Tomography and Magnetic Resonance Imaging of Bone Tumors","volume":"52","author":"Ladd","year":"2017","journal-title":"Semin. Roentgenol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"8625325","DOI":"10.1155\/2019\/8625325","article-title":"Crystallinity and Reinforcement in Poly-L-Lactic Acid Scaffold Induced by Carbon Nanotubes","volume":"2019","author":"Wang","year":"2019","journal-title":"Adv. Polym. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3233","DOI":"10.1016\/j.biomaterials.2011.01.057","article-title":"An overview of tissue and whole organ decellularization processes","volume":"32","author":"Crapo","year":"2011","journal-title":"Biomaterials"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s10561-019-09755-w","article-title":"The use of an acellular matrix derived from human dermis for the treatment of full-thickness skin wounds","volume":"20","author":"Bondioli","year":"2019","journal-title":"Cell Tissue Bank."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1126\/science.1189345","article-title":"Tissue-engineered lungs for in vivo implantation","volume":"329","author":"Petersen","year":"2010","journal-title":"Science"},{"key":"ref_41","first-page":"3675","article-title":"Decellularization of tissues and organs","volume":"27","author":"Gilbert","year":"2006","journal-title":"Biomaterials"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Gilpin, A., and Yang, Y. (2017). Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications. BioMed Res. Int., 2017.","DOI":"10.1155\/2017\/9831534"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.actbio.2016.12.027","article-title":"Human placenta hydrogel reduces scarring in a rat model of cardiac ischemia and enhances cardiomyocyte and stem cell cultures","volume":"52","author":"Francis","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Leonel, L.C.P.C., Miranda, C.M.F.C., Coelho, T.M., Ferreira, G.A.S., Ca\u00e3ada, R.R., Miglino, M.A., and Lobo, S.E. (2018). Decellularization of placentas: Establishing a protocol. Braz. J. Med. Biol. Res., 51.","DOI":"10.1590\/1414-431x20176382"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Barreto, R.S.N., Romagnolli, P., Fratini, P., Mess, A.M., and Miglino, M.A. (2019). Mouse placental scaffolds: A three-dimensional environment model for recellularization. J. Tissue Eng., 10.","DOI":"10.1177\/2041731419867962"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/cyto.a.23242","article-title":"Isolation, cultivation, and characterization of human mesenchymal stem cells","volume":"93","author":"Mushahary","year":"2018","journal-title":"Cytometry. A"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2173","DOI":"10.1002\/sctm.17-0129","article-title":"Concise Review: Multifaceted Characterization of Human Mesenchymal Stem Cells for Use in Regenerative Medicine","volume":"6","author":"Samsonraj","year":"2017","journal-title":"Stem Cells Transl. Med."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Vasanthan, J., Gurusamy, N., Rajasingh, S., Sigamani, V., Kirankumar, S., Thomas, E.L., and Rajasingh, J. (2021). Role of Human Mesenchymal Stem Cells in Regenerative Therapy. Cells, 10.","DOI":"10.3390\/cells10010054"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1111\/j.1532-950X.2006.00197.x","article-title":"Cell Growth Characteristics and Differentiation Frequency of Adherent Equine Bone Marrow\u2013Derived Mesenchymal Stromal Cells: Adipogenic and Osteogenic Capacity","volume":"35","author":"Vidal","year":"2006","journal-title":"Vet. Surg."},{"key":"ref_50","first-page":"14077","article-title":"3D Graphene Oxide-Polyethylenimine Scaffolds for Cardiac Tissue Engineering","volume":"15","author":"Pilato","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.cden.2007.03.004","article-title":"Bone Graft Materials","volume":"51","author":"Precheur","year":"2007","journal-title":"Dent. Clin. N. Am."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1016\/j.cden.2017.06.003","article-title":"Biomaterials For Craniofacial Bone Regeneration","volume":"61","author":"Thrivikraman","year":"2017","journal-title":"Dent. Clin. N. Am."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1838","DOI":"10.1177\/08853282211067646","article-title":"3D printed biocompatible graphene oxide, attapulgite, and collagen composite scaffolds for bone regeneration","volume":"36","author":"Qin","year":"2022","journal-title":"J. Biomater. Appl."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Kyriakides, T.R., Raj, A., Tseng, T.H., Xiao, H., Nguyen, R., Mohammed, F.S., Halder, S., Xu, M., Wu, M.J., and Bao, S. (2021). Biocompatibility of nanomaterials and their immunological properties. Biomed. Mater., 16.","DOI":"10.1088\/1748-605X\/abe5fa"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"35387","DOI":"10.1021\/acsomega.2c03171","article-title":"An Update on Graphene Oxide: Applications and Toxicity","volume":"7","author":"Yadav","year":"2022","journal-title":"ACS Omega"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Biru, E.I., Necolau, M.I., Zainea, A., and Iovu, H. (2022). Graphene Oxide\u2013Protein-Based Scaffolds for Tissue Engineering: Recent Advances and Applications. Polymers, 14.","DOI":"10.3390\/polym14051032"},{"key":"ref_57","first-page":"930","article-title":"Recent advances in two-dimensional nanomaterials for bone tissue engineering","volume":"9","author":"Cao","year":"2023","journal-title":"J. Mater."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.1016\/j.biomaterials.2007.01.046","article-title":"Assessment of bone ingrowth into porous biomaterials using MICRO-CT","volume":"28","author":"Jones","year":"2007","journal-title":"Biomaterials"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1590\/S1806-83242010000400005","article-title":"Evaluation of bone ingrowth into porous titanium implant: Histomorphometric analysis in rabbits","volume":"24","author":"Leite","year":"2010","journal-title":"Braz. Oral Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.progpolymsci.2015.02.004","article-title":"Advancing biomaterials of human origin for tissue engineering","volume":"53","author":"Chen","year":"2016","journal-title":"Prog. Polym. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Capuana, E., Lopresti, F., Ceraulo, M., and La Carrubba, V. (2022). Poly-l-Lactic Acid (PLLA)-Based Biomaterials for Regenerative Medicine: A Review on Processing and Applications. Polymers, 14.","DOI":"10.3390\/polym14061153"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1002\/elsc.202100040","article-title":"The pro-inflammatory response of macrophages regulated by acid degradation products of poly(lactide-co-glycolide) nanoparticles","volume":"21","author":"Ma","year":"2021","journal-title":"Eng. Life Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"S48","DOI":"10.5213\/inj.1734882.441","article-title":"Biodegradation and Biocompatibility of Poly L-lactic Acid Implantable Mesh","volume":"21","author":"Yoon","year":"2017","journal-title":"Int. Neurourol. J."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Liao, C., Li, Y., and Tjong, S.C. (2018). Graphene Nanomaterials: Synthesis, Biocompatibility, and Cytotoxicity. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113564"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.carbon.2018.11.026","article-title":"Properties and behavior of carbon nanomaterials when interfacing neuronal cells: How far have we come?","volume":"143","author":"Rauti","year":"2019","journal-title":"Carbon"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Guo, Z., Chakraborty, S., Monikh, A., Varsou, D.-D., Chetwynd, A.J., Afantitis, A., Lynch, I., Zhang, P., Guo, Z., and Chetwynd, A.J. (2021). Surface Functionalization of Graphene-Based Materials: Biological Behavior, Toxicology, and Safe-By-Design Aspects. Adv. Biol., 5.","DOI":"10.1002\/adbi.202100637"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"115049","DOI":"10.1016\/j.addr.2023.115049","article-title":"Biosafety of mesoporous silica nanoparticles; towards clinical translation","volume":"201","year":"2023","journal-title":"Adv. Drug Deliv. Rev."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/15\/10\/311\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:17:02Z","timestamp":1760113022000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/15\/10\/311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,20]]},"references-count":67,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["jfb15100311"],"URL":"https:\/\/doi.org\/10.3390\/jfb15100311","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,20]]}}}