{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:22:55Z","timestamp":1791840175920,"version":"4.3.4"},"reference-count":62,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T00:00:00Z","timestamp":1739836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key research and development program of Ningxia Hui Autonomous Region","award":["2024BEE02022"],"award-info":[{"award-number":["2024BEE02022"]}]},{"name":"Key research and development program of Ningxia Hui Autonomous Region","award":["69112004"],"award-info":[{"award-number":["69112004"]}]},{"name":"Research and Development of Key Technologies for Soya Protein Processing and Creation of New Products","award":["2024BEE02022"],"award-info":[{"award-number":["2024BEE02022"]}]},{"name":"Research and Development of Key Technologies for Soya Protein Processing and Creation of New Products","award":["69112004"],"award-info":[{"award-number":["69112004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Hydrogel network structures play a crucial role in determining mechanical properties and have broad applications in biomedical and industrial fields. Therefore, their rational design is essential. Herein, we developed a Schiff base-crosslinked hydrogel through the reaction of Tetra-armed polyethylene glycol with aldehyde end groups (Tetra-PEG-CHO) and bovine serum albumin (BSA) under alkaline conditions. In addition, the Tetra-PEG-BSA hydrogel showed a rapid gelation time of around 11 s, much faster than that of the GLU-BSA, HT-BSA, and GDL-BSA hydrogels. It had high optical transmittance (92.92% at 600 nm) and swelling ratios superior to the other gels in different solutions, maintaining structural integrity even in denaturing environments such as guanidine hydrochloride and SDS. Mechanical tests showed superior strain at break (84.12 \u00b1 0.76%), rupture stress (28.64 \u00b1 1.21 kPa), and energy dissipation ability (468.0 \u00b1 34.9 kJ\u00b7m\u22123), surpassing all control group hydrogels. MTT cytotoxicity assays indicated that cell viability remained &gt;80% at lower concentrations, confirming excellent biocompatibility. These findings suggest that Tetra-PEG-BSA hydrogels may serve as effective materials for drug delivery, tissue engineering, and 3D printing.<\/jats:p>","DOI":"10.3390\/jfb16020069","type":"journal-article","created":{"date-parts":[[2025,2,18]],"date-time":"2025-02-18T03:47:06Z","timestamp":1739850426000},"page":"69","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Schiff Base-Crosslinked Tetra-PEG-BSA Hydrogel: Design, Properties, and Multifunctional Functions"],"prefix":"10.3390","volume":"16","author":[{"given":"Yuanyuan","family":"Qu","sequence":"first","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua Donglu, Haidian District, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2279-9506","authenticated-orcid":false,"given":"Jinlong","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chemistry and Materials Engineering, Beijing Technology and Business University, 11 Fucheng Road, Haidian District, Beijing 100048, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7644-5813","authenticated-orcid":false,"given":"Xin","family":"Jia","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua Donglu, Haidian District, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4508-3906","authenticated-orcid":false,"given":"Lijun","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua Donglu, Haidian District, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.ijbiomac.2019.08.258","article-title":"Natural protein-based hydrogels with high strength and rapid self-recovery","volume":"141","author":"Liu","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1089\/ten.teb.2012.0458","article-title":"Protein\u2013hydrogel interactions in tissue engineering: Mechanisms and applications","volume":"19","author":"Zustiak","year":"2013","journal-title":"Tissue Eng. Part B Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2314079","DOI":"10.1002\/adfm.202314079","article-title":"Electroactive biomaterials regulate the electrophysiological microenvironment to promote bone and cartilage tissue regeneration","volume":"34","author":"Chen","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2315509","DOI":"10.1002\/adfm.202315509","article-title":"Protein-Based Controllable Nanoarchitectonics for Desired Applications","volume":"34","author":"Li","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1021\/cm202640w","article-title":"Peptide-and protein-based hydrogels","volume":"24","author":"Jonker","year":"2011","journal-title":"Chem. Mater."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1002\/jbm.a.36534","article-title":"Preparation, characterization and application of a protein hydrogel with rapid self-healing and unique autofluoresent multi-functionalities","volume":"107","author":"Chen","year":"2019","journal-title":"J. Biomed. Mater. Res. A"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"127867","DOI":"10.1016\/j.colsurfa.2021.127867","article-title":"Dual-network sodium alginate\/polyacrylamide\/laponite nanocomposite hydrogels with high toughness and cyclic mechano-responsiveness","volume":"633","author":"Chen","year":"2022","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"107737","DOI":"10.1016\/j.foodhyd.2022.107737","article-title":"A combined enzymatic and ionic cross-linking strategy for pea protein\/sodium alginate double-network hydrogel with excellent mechanical properties and freeze-thaw stability","volume":"131","author":"YWang","year":"2022","journal-title":"Food Hydrocoll."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"124842","DOI":"10.1016\/j.polymer.2022.124842","article-title":"Dramatically enhancing mechanical properties of hydrogels by drying reactive polymers at elevated temperatures to introduce strong physical and chemical crosslinks","volume":"249","author":"Wu","year":"2022","journal-title":"Polymer"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"105865","DOI":"10.1016\/j.foodhyd.2020.105865","article-title":"Corn fiber gum-soybean protein isolate double network hydrogel as oral delivery vehicles for thermosensitive bioactive compounds","volume":"107","author":"Yan","year":"2020","journal-title":"Food Hydrocoll."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.ijbiomac.2022.03.128","article-title":"Tea polyphenol\/glycerol-treated double-network hydrogel with enhanced mechanical stability and anti-drying, antioxidant and antibacterial properties for accelerating wound healing","volume":"208","author":"Dong","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.ijbiomac.2022.01.057","article-title":"Bio-based poly (\u03b3-glutamic acid)-gelatin double-network hydrogel with high strength for wound healing","volume":"202","author":"Dou","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.matlet.2019.06.102","article-title":"Preparing antibacterial and in-situ formable double crosslinking chitosan\/hyaluronan composite hydrogels","volume":"254","author":"Hu","year":"2019","journal-title":"Mater. Lett."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"102757","DOI":"10.1016\/j.mtcomm.2021.102757","article-title":"Research progress on double-network hydrogels","volume":"29","author":"Huang","year":"2021","journal-title":"Mater. Today Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"111590","DOI":"10.1016\/j.msec.2020.111590","article-title":"Thermoresponsive BSA hydrogels with phase tunability","volume":"119","author":"Khanna","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_16","first-page":"104457","article-title":"Development of BSA gel\/Pectin\/Chitosan polyelectrolyte complex microcapsules for Berberine delivery and evaluation of their inhibitory effect on Cutibacterium acnes","volume":"147","author":"Bogdan","year":"2019","journal-title":"React. Funct. Polym."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0308-8146(96)00009-X","article-title":"A novel titration methodology for elucidation of the structure of preformed cooked cured-meat pigment by visible spectroscopy","volume":"56","author":"Pegg","year":"1996","journal-title":"Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.jconrel.2022.01.035","article-title":"PCL-PEG copolymer based injectable thermosensitive hydrogels","volume":"343","author":"Dethe","year":"2022","journal-title":"J. Control. Release"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1016\/j.reactfunctpolym.2013.03.015","article-title":"Gelation mechanism and mechanical properties of Tetra-PEG gel","volume":"73","author":"Sakai","year":"2013","journal-title":"React. Funct. Polym."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.ijbiomac.2021.03.071","article-title":"An in situ mechanical adjustable double crosslinking hyaluronic acid\/poly-lysine hydrogel matrix: Fabrication, characterization and cell morphology","volume":"180","author":"Guo","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.jiec.2021.01.041","article-title":"Development of bioresorbable smart injectable hydrogels based on thermo-responsive copolymer integrated bovine serum albumin bioconjugates for accelerated healing of excisional wounds","volume":"96","author":"Phan","year":"2021","journal-title":"J. Ind. Eng. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Ma, X., Zhang, Z., Zheng, Y., and Liu, J. (2024). Solid-Phase Electrochemiluminescence Enzyme Electrodes Based on Nanocage Arrays for Highly Sensitive Detection of Cholesterol. Biosensors, 14.","DOI":"10.3390\/bios14080403"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"109591","DOI":"10.1016\/j.jfluchem.2020.109591","article-title":"Role of percent grafting and chain length of fully fluorinated pendant units in the grafted acrylic compound on crucial characteristic properties of high density polyethylene","volume":"236","author":"Soykan","year":"2020","journal-title":"J. Fluor. Chem."},{"key":"ref_24","unstructured":"Brotherton, E. (2022). Synthesis and Applications of New Hydrophilic Aldehyde-Functional Methacrylic Polymers, University of Sheffield."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e00170","DOI":"10.1016\/j.bprint.2021.e00170","article-title":"3D printing of gelatin\/chitosan biodegradable hybrid hydrogel: Critical issues due to the crosslinking reaction, degradation phenomena and process parameters","volume":"24","author":"Giorleo","year":"2021","journal-title":"Bioprinting"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1021\/acs.bioconjchem.9b00236","article-title":"Protein PEPylation: A new paradigm of protein\u2013polymer conjugation","volume":"30","author":"Hou","year":"2019","journal-title":"Bioconjugate Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"12864","DOI":"10.1021\/acs.chemrev.1c01015","article-title":"Engineered Hemostatic Biomaterials for Sealing Wounds","volume":"122","author":"Montazerian","year":"2022","journal-title":"Chem. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"31519","DOI":"10.1038\/s41598-024-83275-w","article-title":"Preparation and characterization of PEG-Dex macromolecular schiff base particles and their application on the stabilization of water-in-water emulsion","volume":"14","author":"Yang","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_29","unstructured":"Alaneed, R. (2022). Enzymatic Synthesis of Polyesters and Their Application in Protein-Polymer Conjugation and Networks Formation: Kumulative Dissertation. [Ph.D. Thesis, Martin-Luther-Universit\u00e4t Halle-Wittenberg]."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"107997","DOI":"10.1016\/j.foodhyd.2022.107997","article-title":"Effect of calcium ions concentration on the properties and microstructures of doubly induced sorghum arabinoxylan\/soy protein isolate mixed gels","volume":"133","author":"Yan","year":"2022","journal-title":"Food Hydrocoll."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Chen, G., Tang, W., Wang, X., Zhao, X., Chen, C., and Zhu, Z. (2019). Applications of hydrogels with special physical properties in biomedicine. Polymers, 11.","DOI":"10.3390\/polym11091420"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"128956","DOI":"10.1016\/j.foodchem.2020.128956","article-title":"Structural, gelation properties and microstructure of rice glutelin\/sugar beet pectin composite gels: Effects of ionic strengths","volume":"346","author":"Wang","year":"2021","journal-title":"Food Chem."},{"key":"ref_33","first-page":"6","article-title":"Swell gel for natural drug delivery","volume":"19","author":"Bradley","year":"2015","journal-title":"Mater. Today"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"105200","DOI":"10.1016\/j.reactfunctpolym.2022.105200","article-title":"Hydrogel-based microneedles of chitosan derivatives for drug delivery","volume":"172","author":"Wei","year":"2022","journal-title":"React. Funct. Polym."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"13165","DOI":"10.1021\/acs.chemrev.5b00299","article-title":"Supramolecular Hydrogelators and Hydrogels: From Soft Matter to Molecular Biomaterials","volume":"115","author":"Du","year":"2015","journal-title":"Chem. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"126728","DOI":"10.1016\/j.foodchem.2020.126728","article-title":"Gel properties of myofibrillar proteins heated at different heating rates under a low-frequency magnetic field","volume":"321","author":"Wang","year":"2020","journal-title":"Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"133596","DOI":"10.1016\/j.ijbiomac.2024.133596","article-title":"Preparation of bovine serum albumin-arabinoxylan cold-set gels by glucono-\u03b4-lactone and salt ions double induction","volume":"277","author":"Shen","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1007\/s11947-022-02903-3","article-title":"Cross-linked bovine serum albumin-Crocin I nanoparticle-based gel network for stabilizing high internal phase Pickering emulsion","volume":"15","author":"Huang","year":"2022","journal-title":"Food Bioprocess Technol."},{"key":"ref_39","unstructured":"Plesner, B. (2011). Biophysical Characterisation of PEGylated Proteins and Highly Concentrated Protein Solutions, Roskilde University."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"685690","DOI":"10.1155\/2015\/685690","article-title":"Fibrin gel as an injectable biodegradable scaffold and cell carrier for tissue engineering","volume":"2015","author":"Li","year":"2015","journal-title":"Sci. World J."},{"key":"ref_41","unstructured":"Colombo, G. (2019). Controlling Microstructure and Rheological Properties of Colloidal Gels, ETH Zurich."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4472","DOI":"10.1039\/C7PY01035D","article-title":"Origin of nanostructural inhomogeneity in polymer-network gels","volume":"8","author":"Seiffert","year":"2017","journal-title":"Polym. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1179\/1743280414Y.0000000032","article-title":"Fibre-reinforced hydrogels with high optical transparency","volume":"59","author":"Maranchi","year":"2014","journal-title":"Int. Mater. Rev."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"118871","DOI":"10.1016\/j.carbpol.2021.118871","article-title":"A 4arm-PEG macromolecule crosslinked chitosan hydrogels as antibacterial wound dressing","volume":"277","author":"Lu","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2405255","DOI":"10.1002\/adfm.202405255","article-title":"Multifunctional hydrogel based on silk fibroin promotes tissue repair and regeneration","volume":"34","author":"Lin","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e2303326","DOI":"10.1002\/advs.202303326","article-title":"Tailoring the Swelling-Shrinkable Behavior of Hydrogels for Biomedical Applications","volume":"10","author":"Feng","year":"2023","journal-title":"Adv. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"301","DOI":"10.2478\/v10007-007-0024-6","article-title":"Swelling behavior and release properties of pH-sensitive hydrogels based on methacrylic derivatives","volume":"57","author":"Bartil","year":"2007","journal-title":"Acta Pharm."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"117978","DOI":"10.1016\/j.carbpol.2021.117978","article-title":"Topological cyclodextrin nanoparticles as crosslinkers for self-healing tough hydrogels as strain sensors","volume":"264","author":"Chen","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jmst.2021.05.020","article-title":"Tetradic double-network physical crosslinking hydrogels with synergistic high stretchable, self-healing, adhesive, and strain-sensitive properties","volume":"98","author":"Bai","year":"2021","journal-title":"J. Mater. Sci. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"19","DOI":"10.47583\/ijpsrr.2021.v69i02.002","article-title":"Modalities of protein denaturation and nature of denaturants","volume":"69","author":"Acharya","year":"2021","journal-title":"Int. J. Pharm. Sci. Rev. Res."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"672","DOI":"10.1039\/C3SM52272E","article-title":"Multi-scale multi-mechanism design of tough hydrogels: Building dissipation into stretchy networks","volume":"10","author":"Zhao","year":"2013","journal-title":"Soft Matter"},{"key":"ref_52","first-page":"285","article-title":"Dissolution and swelling of soy protein isolate hydrogels in alkali","volume":"56","author":"Zhao","year":"2015","journal-title":"Food Hydrocoll."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"110011","DOI":"10.1016\/j.foodhyd.2024.110011","article-title":"Optimizing texture and mechanical properties: The impact of pH-modulated metal-phenolic networks on soy protein isolate gels","volume":"153","author":"Lan","year":"2024","journal-title":"Food Hydrocoll."},{"key":"ref_54","unstructured":"Anson, M.L., and Edsall, J.T. (1948). Protein Gels11Supported in part by the Research Committee of the Graduate School of the University of Wisconsin from funds supplied by the Wisconsin Alumni Research Foundation. Advances in Protein Chemistry, Academic Press."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.actbio.2021.10.044","article-title":"Kruzic, Structural aspects controlling the mechanical and biological properties of tough, double network hydrogels","volume":"138","author":"Huang","year":"2022","journal-title":"Acta Biomater."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"113386","DOI":"10.1016\/j.lwt.2022.113386","article-title":"Effect of microwave-ultrasonic combination treatment on heating-induced gel properties of low-sodium tilapia surimi during gel setting stage and comparative analysis","volume":"161","author":"Ye","year":"2022","journal-title":"LWT"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"285","DOI":"10.3109\/03008207.2011.640760","article-title":"Structural mechanism for alteration of collagen gel mechanics by glutaraldehyde crosslinking","volume":"53","author":"Chandran","year":"2011","journal-title":"Connect. Tissue Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.carbpol.2008.07.016","article-title":"Gelation of high methoxy pectin by acidification with d-glucono-\u03b4-lactone (GDL) at room temperature","volume":"75","author":"Agoub","year":"2009","journal-title":"Carbohydr. Polym."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2102557","DOI":"10.1002\/advs.202102557","article-title":"A General Protein Unfolding-Chemical Coupling Strategy for Pure Protein Hydrogels with Mechanically Strong and Multifunctional Properties","volume":"9","author":"Tang","year":"2021","journal-title":"Adv. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"715","DOI":"10.4103\/pm.pm_151_19","article-title":"Antitumor effects and mechanism of protein from Panax ginseng CA Meyer on human breast cancer cell line MCF-7","volume":"15","author":"Wan","year":"2019","journal-title":"Pharmacogn. Mag."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"104016","DOI":"10.1016\/j.jddst.2022.104016","article-title":"Preparation and characterization of magnetic PEG-PEI-PLA-PEI-PEG\/Fe3O4-PCL\/DNA micelles for gene delivery into MCF-7 cells","volume":"79","author":"Afrouz","year":"2022","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"35469","DOI":"10.1039\/C4RA13581D","article-title":"Injectable hydrogels based on poly (ethylene glycol) and derivatives as functional biomaterials","volume":"5","author":"Bakaic","year":"2015","journal-title":"RSC Adv."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/16\/2\/69\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:36:31Z","timestamp":1760027791000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/16\/2\/69"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,18]]},"references-count":62,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["jfb16020069"],"URL":"https:\/\/doi.org\/10.3390\/jfb16020069","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,18]]}}}