{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:57:35Z","timestamp":1791583055072,"version":"4.3.4"},"reference-count":46,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T00:00:00Z","timestamp":1751068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Effective bone tissue regeneration remains pivotal in implant dentistry, particularly for edentulous patients with compromised alveolar bone due to atrophy and sinus pneumatization. Biomaterials are essential for promoting regenerative processes by supporting cellular recruitment, vascularization, and osteogenesis. This study presents the development and characterization of a novel lithography-printed ceramic \u03b2-TCP scaffold, with a macro\/micro-porous lattice, engineered to optimize osteoconduction and mechanical stability. Morphological, structural, and biomechanical assessments confirmed a reproducible microarchitecture with suitable porosity and load-bearing capacity. The scaffold was also employed for maxillary sinus augmentation, with postoperative evaluation using micro computed tomography, synchrotron imaging, histology, and Fourier Transform Infrared Imaging analysis, demonstrating active bone regeneration, scaffold resorption, and formation of mineralized tissue. Advanced imaging supported by deep learning tools revealed a well-organized osteocyte network and high-quality bone, underscoring the scaffold\u2019s biocompatibility and osteoconductive efficacy. These findings support the application of these 3D-printed \u03b2-TCP scaffolds in regenerative dental medicine, facilitating tissue regeneration in complex jawbone deficiencies.<\/jats:p>","DOI":"10.3390\/jfb16070237","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T10:03:48Z","timestamp":1751277828000},"page":"237","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Morphometric, Biomechanical and Macromolecular Performances of \u03b2-TCP Macro\/Micro-Porous Lattice Scaffolds Fabricated via Lithography-Based Ceramic Manufacturing for Jawbone Engineering"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4419-4674","authenticated-orcid":false,"given":"Carlo","family":"Mangano","sequence":"first","affiliation":[{"name":"Department of Dental Sciences, University Vita Salute San Raffaele, 20132 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4798-5236","authenticated-orcid":false,"given":"Nicole","family":"Riberti","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Sciences, Marche Polytechnic University, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Orilisi","sequence":"additional","affiliation":[{"name":"Department of Clinical Specialistic and Dental Sciences, Marche Polytechnic University, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2986-1114","authenticated-orcid":false,"given":"Simona","family":"Tecco","sequence":"additional","affiliation":[{"name":"I.R.C.C.S. San Raffaele Hospital, University Vita Salute San Raffaele, 20132 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9261-2682","authenticated-orcid":false,"given":"Michele","family":"Furlani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health, Marche Polytechnic University, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8451-2569","authenticated-orcid":false,"given":"Christian","family":"Giommi","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Science, Marche Polytechnic University, 60131 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9049-8524","authenticated-orcid":false,"given":"Paolo","family":"Mengucci","sequence":"additional","affiliation":[{"name":"Department Science and Engineering of Materials, Environment and Urban Planning, Marche Polytechnic University, 60131 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0503-5870","authenticated-orcid":false,"given":"Elisabetta","family":"Giorgini","sequence":"additional","affiliation":[{"name":"Department of Life and Environmental Science, Marche Polytechnic University, 60131 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4177-7441","authenticated-orcid":false,"given":"Alessandra","family":"Giuliani","sequence":"additional","affiliation":[{"name":"Department of Clinical Specialistic and Dental Sciences, Marche Polytechnic University, 60126 Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.2174\/1389201017666161221155237","article-title":"Molecular, Cellular and Pharmaceutical Aspects of Bone Grafting Materials and Membranes During Maxillary Sinus-Lift Procedures. Part 1: A General Overview","volume":"18","author":"Iezzi","year":"2017","journal-title":"Curr. Pharm. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhao, R., Yang, R., Cooper, P.R., Khurshid, Z., Shavandi, A., and Ratnayake, J. (2021). Bone Grafts and Substitutes in Dentistry: A Review of Current Trends and Developments. Molecules, 26.","DOI":"10.3390\/molecules26103007"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"012033","DOI":"10.1088\/1742-6596\/2837\/1\/012033","article-title":"A Systematic Review on Bone Grafts and Biomaterials Substitutes for Bone Regeneration","volume":"2837","author":"Chaudhari","year":"2024","journal-title":"J. Phys. Conf. Ser."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Todd, E.A., Mirsky, N.A., Silva, B.L.G., Shinde, A.R., Arakelians, A.R.L., Nayak, V.V., Marcantonio, R.A.C., Gupta, N., Witek, L., and Coelho, P.G. (2024). Functional Scaffolds for Bone Tissue Regeneration: A Comprehensive Review of Materials, Methods, and Future Directions. J. Funct. Biomater., 15.","DOI":"10.3390\/jfb15100280"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Gatto, M.L., Cerqueni, G., Furlani, M., Riberti, N., Tognoli, E., Denti, L., Leonardi, F., Giuliani, A., Mattioli-Belmonte, M., and Mengucci, P. (2023). Influence of Trabecular Geometry on Scaffold Mechanical Behavior and MG-63 Cell Viability. Materials, 16.","DOI":"10.3390\/ma16062342"},{"key":"ref_6","first-page":"271","article-title":"Recent Advances in Biomaterials for 3D Scaffolds: A Review","volume":"4","author":"Nikolova","year":"2019","journal-title":"Bioact. Mater."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Farjaminejad, S., Farjaminejad, R., Hasani, M., Garcia-Godoy, F., Abdouss, M., Marya, A., Harsoputranto, A., and Jamilian, A. (2024). Advances and Challenges in Polymer-Based Scaffolds for Bone Tissue Engineering: A Path Towards Personalized Regenerative Medicine. Polymers, 16.","DOI":"10.3390\/polym16233303"},{"key":"ref_8","first-page":"54","article-title":"Biodegradable Materials for Bone Defect Repair","volume":"7","author":"Wei","year":"2020","journal-title":"Mil. Med. Res."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Bahraminasab, M. (2020). Challenges on Optimization of 3D-Printed Bone Scaffolds. Biomed. Eng. Online, 19.","DOI":"10.1186\/s12938-020-00810-2"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Mangano, C., Mangano, F., Gobbi, L., Admakin, O., Iketani, S., and Giuliani, A. (2019). Comparative Study between Laser Light Stereo-Lithography 3D-Printed and Traditionally Sintered Biphasic Calcium Phosphate Scaffolds by an Integrated Morphological, Morphometric and Mechanical Analysis. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20133118"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e7171","DOI":"10.1002\/ccr3.7171","article-title":"Alveolar Ridge Augmentation with 3D-Printed Synthetic Bone Blocks: A Clinical Case Series","volume":"11","author":"Perez","year":"2023","journal-title":"Clin. Case Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1186\/s13063-022-06843-3","article-title":"The Use of 3D Ceramic Block Graft Compared with Autogenous Block Graft for Rehabilitation of the Atrophic Maxilla: A Randomized Controlled Clinical Trial","volume":"23","author":"Possari","year":"2022","journal-title":"Trials"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ghayor, C., Chen, T.-H., Bhattacharya, I., \u00d6zcan, M., and Weber, F.E. (2020). Microporosities in 3D-Printed Tricalcium-Phosphate-Based Bone Substitutes Enhance Osteoconduction and Affect Osteoclastic Resorption. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21239270"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2702","DOI":"10.1016\/j.matpr.2022.06.498","article-title":"Recent Methods in Fabricating Porous \u03b2-Tricalcium Phosphate Scaffolds: A Mini Review","volume":"66","author":"Mohammed","year":"2022","journal-title":"Mater. Today Proc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s40729-024-00541-2","article-title":"Patient-Specific Beta-Tricalcium Phosphate Scaffold for Customized Alveolar Ridge Augmentation: A Case Report","volume":"10","author":"Essig","year":"2024","journal-title":"Int. J. Implant. Dent."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1007\/s10856-021-06569-9","article-title":"Development of Bioinks for 3D Printing Microporous, Sintered Calcium Phosphate Scaffolds","volume":"32","author":"Montelongo","year":"2021","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1111\/prd.12525","article-title":"3D Printing for Bone Regeneration: Challenges and Opportunities for Achieving Predictability","volume":"93","author":"Ivanovski","year":"2023","journal-title":"Periodontol. 2000"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.1089\/ten.tea.2018.0014","article-title":"Lattice Microarchitecture for Bone Tissue Engineering from Calcium Phosphate Compared to Titanium","volume":"24","author":"Chen","year":"2018","journal-title":"Tissue Eng. Part. A"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/ijac.12319","article-title":"Additive Manufacturing of Dense Alumina Ceramics","volume":"12","author":"Schwentenwein","year":"2015","journal-title":"Int. J. Appl. Ceram. Technol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0011-8532(22)02107-3","article-title":"Maxillary and Sinus Implant Reconstructions","volume":"30","author":"Tatum","year":"1986","journal-title":"Dent. Clin. N. Am."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1107\/S0021889809008309","article-title":"ReX: A Computer Program for Structural Analysis Using Powder Diffraction Data","volume":"42","author":"Bortolotti","year":"2009","journal-title":"J. Appl. Crystallogr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1002\/(SICI)1096-9896(199601)178:1<100::AID-PATH429>3.0.CO;2-K","article-title":"Fractal Dimension and Architecture of Trabecular Bone","volume":"178","author":"Fazzalari","year":"1996","journal-title":"J. Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s40679-016-0036-8","article-title":"SYRMEP Tomo Project: A Graphical User Interface for Customizing CT Reconstruction Workflows","volume":"3","author":"Brun","year":"2017","journal-title":"Adv. Struct. Chem. Imaging"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1046\/j.1365-2818.2002.01010.x","article-title":"Simultaneous Phase and Amplitude Extraction from a Single Defocused Image of a Homogeneous Object","volume":"206","author":"Paganin","year":"2002","journal-title":"J. Microsc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1017\/S143192761800315X","article-title":"Dragonfly as a Platform for Easy Image-Based Deep Learning Applications","volume":"24","author":"Makovetsky","year":"2018","journal-title":"Microsc. Microanal."},{"key":"ref_27","unstructured":"Riberti, N. (2025). Artificial Intelligence in Dentistry: Innovative Tools and Advanced Analysis of Bone and Soft Tissues. [Ph.D. Thesis, University G.d\u2019Annunzio]."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1107\/S1600577523009852","article-title":"Deep Learning to Overcome Zernike Phase-Contrast NanoCT Artifacts for Automated Micro-Nano Porosity Segmentation in Bone","volume":"31","author":"Silveira","year":"2024","journal-title":"J. Synchrotron Radiat."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/s11914-015-0256-1","article-title":"Osteocyte Shape and Mechanical Loading","volume":"13","author":"Wang","year":"2015","journal-title":"Curr. Osteoporos. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1038\/s41368-017-0007-5","article-title":"Osteocyte Morphology and Orientation in Relation to Strain in the Jaw Bone","volume":"10","author":"Wu","year":"2018","journal-title":"Int. J. Oral. Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.imavis.2004.06.012","article-title":"Surface Area Estimation of Digitized 3D Objects Using Weighted Local Configurations","volume":"23","author":"Lindblad","year":"2005","journal-title":"Image Vis. Comput."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.bone.2007.10.021","article-title":"Bone Mineralization Density Distribution in Health and Disease","volume":"42","author":"Roschger","year":"2008","journal-title":"Bone"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1080\/10520290701333558","article-title":"A Two-Color Acid-Free Cartilage and Bone Stain for Zebrafish Larvae","volume":"82","author":"Walker","year":"2007","journal-title":"Biotech. Histochem."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Zadka, \u0141., Chrabaszcz, K., Buzalewicz, I., Wiercigroch, E., Glatzel-Pluci\u0144ska, N., Szleszkowski, \u0141., Gomu\u0142kiewicz, A., Piotrowska, A., Kurnol, K., and Dzi\u0119giel, P. (2021). Molecular profiling of the intestinal mucosa and immune cells of the colon by multi-parametric histological techniques. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-90761-y"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Belloni, A., Montanari, E., Sagrati, A., Lorenzi, T., Balloni, A., Busard\u00f2, F.P., Notarstefano, V., Fabri, M., and Giorgini, E. (2023). Novel Insights from Fourier-Transform InfraRed Imaging on the Morpho-Chemical Profile of Human Corpus Callosum. Appl. Sci., 13.","DOI":"10.3390\/app13063954"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"110303","DOI":"10.1016\/j.isci.2024.110303","article-title":"Biomolecular Alterations Temporally Anticipate Microarchitectural Modifications of Collagen in Oral Tongue Squamous Cell Carcinoma","volume":"27","author":"Togni","year":"2024","journal-title":"iScience"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"37","DOI":"10.12688\/wellcomeopenres.16619.1","article-title":"BoneJ2\u2014Refactoring Established Research Software","volume":"6","author":"Domander","year":"2021","journal-title":"Wellcome Open Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Xidaki, D., Agrafioti, P., Diomatari, D., Kaminari, A., Tsalavoutas-Psarras, E., Alexiou, P., Psycharis, V., Tsilibary, E., Silvestros, S., and Sagnou, M. (2018). Synthesis of Hydroxyapatite, \u03b2-Tricalcium Phosphate and Biphasic Calcium Phosphate Particles to Act as Local Delivery Carriers of Curcumin: Loading, Release and In Vitro Studies. Materials, 11.","DOI":"10.3390\/ma11040595"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2170","DOI":"10.1007\/s11999-010-1751-4","article-title":"Infrared Assessment of Bone Quality: A Review","volume":"469","author":"Paschalis","year":"2011","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Castorina, F., Masi, U., Giorgini, E., Mori, L., Tafuri, M.A., and Notarstefano, V. (2023). Evidence for Mild Diagenesis in Archaeological Human Bones from the Fewet Necropolis (SW Libya): New Insights and Implications from ATR\u2013FTIR Spectroscopy. Appl. Sci., 13.","DOI":"10.3390\/app13020687"},{"key":"ref_41","first-page":"87","article-title":"The Treatment of Sinus Graft Infection after Sinus Floor Elevation: A Series of Four Case Reports","volume":"50","author":"Liu","year":"2024","journal-title":"J. Oral Implantol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Park, W.-B., Okany, K.P., Park, W., Han, J.-Y., Lim, H.-C., and Kang, P. (2024). Atypical and Late-Developed Sinus Graft Complications Following Maxillary Sinus Augmentation: Successful Management with Guided Bone Regeneration. Medicina, 60.","DOI":"10.3390\/medicina60081246"},{"key":"ref_43","first-page":"e30455","article-title":"The Etiology and Management of Dental Implant Failure: A Review","volume":"14","author":"Kochar","year":"2022","journal-title":"Cureus"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"\u0141uczak, J.W., Palusi\u0144ska, M., Matak, D., Pietrzak, D., Nakielski, P., Lewicki, S., Grodzik, M., and Szyma\u0144ski, \u0141. (2024). The Future of Bone Repair: Emerging Technologies and Biomaterials in Bone Regeneration. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms252312766"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1021\/ja01269a023","article-title":"Adsorption of Gases in Multimolecular Layers","volume":"60","author":"Brunauer","year":"1938","journal-title":"J. Am. Chem. Soc."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e2105194","DOI":"10.1002\/advs.202105194","article-title":"Transcriptome Analysis Revealed the Symbiosis Niche of 3D Scaffolds to Accelerate Bone Defect Healing","volume":"9","author":"Ji","year":"2022","journal-title":"Adv. Sci."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/16\/7\/237\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:01:05Z","timestamp":1760032865000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/16\/7\/237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,28]]},"references-count":46,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["jfb16070237"],"URL":"https:\/\/doi.org\/10.3390\/jfb16070237","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,28]]}}}