{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T03:09:13Z","timestamp":1779332953250,"version":"3.51.4"},"reference-count":36,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of P\u00e9cs, Medical Faculty","award":["\u201c\u00c1OK-TANDEM_palyazat_2019_09_16_ Turz\u00f3 Kinga\u201d"],"award-info":[{"award-number":["\u201c\u00c1OK-TANDEM_palyazat_2019_09_16_ Turz\u00f3 Kinga\u201d"]}]},{"name":"University of P\u00e9cs, Medical Faculty","award":["\u201c\u00c1OK-TANDEM_palyazat_2023_04_04_Turz\u00f3 Kinga\u201d"],"award-info":[{"award-number":["\u201c\u00c1OK-TANDEM_palyazat_2023_04_04_Turz\u00f3 Kinga\u201d"]}]},{"name":"University of P\u00e9cs, Medical Faculty","award":["TKP2021-NVA-06"],"award-info":[{"award-number":["TKP2021-NVA-06"]}]},{"name":"University of P\u00e9cs, Medical Faculty","award":["2022-2.1.1-NL-2022-00012"],"award-info":[{"award-number":["2022-2.1.1-NL-2022-00012"]}]},{"name":"National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-NVA funding scheme","award":["\u201c\u00c1OK-TANDEM_palyazat_2019_09_16_ Turz\u00f3 Kinga\u201d"],"award-info":[{"award-number":["\u201c\u00c1OK-TANDEM_palyazat_2019_09_16_ Turz\u00f3 Kinga\u201d"]}]},{"name":"National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-NVA funding scheme","award":["\u201c\u00c1OK-TANDEM_palyazat_2023_04_04_Turz\u00f3 Kinga\u201d"],"award-info":[{"award-number":["\u201c\u00c1OK-TANDEM_palyazat_2023_04_04_Turz\u00f3 Kinga\u201d"]}]},{"name":"National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-NVA funding scheme","award":["TKP2021-NVA-06"],"award-info":[{"award-number":["TKP2021-NVA-06"]}]},{"name":"National Research, Development and Innovation Fund of Hungary, financed under the TKP2021-NVA funding scheme","award":["2022-2.1.1-NL-2022-00012"],"award-info":[{"award-number":["2022-2.1.1-NL-2022-00012"]}]},{"name":"Ministry of Culture and Innovation from the National Research, Development and Innovation Fund","award":["\u201c\u00c1OK-TANDEM_palyazat_2019_09_16_ Turz\u00f3 Kinga\u201d"],"award-info":[{"award-number":["\u201c\u00c1OK-TANDEM_palyazat_2019_09_16_ Turz\u00f3 Kinga\u201d"]}]},{"name":"Ministry of Culture and Innovation from the National Research, Development and Innovation Fund","award":["\u201c\u00c1OK-TANDEM_palyazat_2023_04_04_Turz\u00f3 Kinga\u201d"],"award-info":[{"award-number":["\u201c\u00c1OK-TANDEM_palyazat_2023_04_04_Turz\u00f3 Kinga\u201d"]}]},{"name":"Ministry of Culture and Innovation from the National Research, Development and Innovation Fund","award":["TKP2021-NVA-06"],"award-info":[{"award-number":["TKP2021-NVA-06"]}]},{"name":"Ministry of Culture and Innovation from the National Research, Development and Innovation Fund","award":["2022-2.1.1-NL-2022-00012"],"award-info":[{"award-number":["2022-2.1.1-NL-2022-00012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Bioactive glass materials have been used for decades in orthopedic surgery, traumatology, and oral and maxillofacial surgery to repair bone defects. This study aimed to evaluate in vitro the survival and proliferation of MG63 human osteosarcoma-derived cells on S53P4 bioactive glass (BonAlive\u00ae granules). Microscopic visualization was performed to directly observe the interactions between the cells and the material. Osteoblast-like cells were examined on non-adherent test plates, on tissue culture (TC)-treated plates and on the surface of the bioglass to assess the differences. Cell survival and proliferation were monitored using a CCK-8 optical density assay. Comparing the mean OD of MG63 cells in MEM on TC-treated plates with cells on BG, we detected a significant difference (p &lt; 0.05), over each time of observation. The sustained cell proliferation confirmed the non-cytotoxic property of the bioglass, as the cell number increased continuously at 48, 72, 96, and 168 h and even did not plateau after 168 h. Since the properties of bioglasses can vary significantly depending on their composition and environment, a thorough characterization of their biocompatibility is crucial to ensure their effective and appropriate application\u2014for example, during hip and knee prosthesis insertion.<\/jats:p>","DOI":"10.3390\/jfb16080275","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T16:16:10Z","timestamp":1753805770000},"page":"275","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interaction of MG63 Human Osteosarcoma-Derived Cells on S53P4 Bioactive Glass: An In Vitro Study"],"prefix":"10.3390","volume":"16","author":[{"given":"Valentin","family":"Schmidt","sequence":"first","affiliation":[{"name":"Department of Traumatology and Hand Surgery, Faculty of Medicine, University of P\u00e9cs, Ifj\u00fas\u00e1g Str. 13, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8687-7430","authenticated-orcid":false,"given":"Be\u00e1ta","family":"Polg\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Faculty of Medicine, University of P\u00e9cs, Szigeti Str. 12, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7504-2390","authenticated-orcid":false,"given":"Vanda \u00c1gnes","family":"Nemes","sequence":"additional","affiliation":[{"name":"Department of Traumatology and Hand Surgery, Faculty of Medicine, University of P\u00e9cs, Ifj\u00fas\u00e1g Str. 13, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1096-775X","authenticated-orcid":false,"given":"T\u00edmea","family":"Dergez","sequence":"additional","affiliation":[{"name":"Insitute of Bioanalysis, Faculty of Medicine, University of P\u00e9cs, Honv\u00e9d Str. 1, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2066-319X","authenticated-orcid":false,"given":"L\u00e1szl\u00f3","family":"Janov\u00e1k","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry and Materials Science, University of Szeged, Rerrich B\u00e9la Square 1, H-6721 Szeged, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7538-0675","authenticated-orcid":false,"given":"P\u00e9ter","family":"Mar\u00f3ti","sequence":"additional","affiliation":[{"name":"3D Printing and Visualisation Centre, University of P\u00e9cs, Boszork\u00e1ny Str. 2, H-7624 P\u00e9cs, Hungary"},{"name":"Medical Simulation Education Centre, Faculty of Medicine, University of P\u00e9cs, Szigeti Str. 12, H-7624 Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Szil\u00e1rd","family":"Rendeki","sequence":"additional","affiliation":[{"name":"Medical Simulation Education Centre, Faculty of Medicine, University of P\u00e9cs, Szigeti Str. 12, H-7624 Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4133-3998","authenticated-orcid":false,"given":"Kinga","family":"Turz\u00f3","sequence":"additional","affiliation":[{"name":"Dental School, Faculty of Medicine, University of P\u00e9cs, T\u00fcz\u00e9r u.1, H-7623 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0318-7020","authenticated-orcid":false,"given":"Bal\u00e1zs","family":"Patczai","sequence":"additional","affiliation":[{"name":"Department of Traumatology and Hand Surgery, Faculty of Medicine, University of P\u00e9cs, Ifj\u00fas\u00e1g Str. 13, H-7624 P\u00e9cs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1007\/s42765-024-00481-x","article-title":"Bioactive Glass-Reinforced Hybrid Microfibrous Spheres Promote Bone Defect Repair via Stem Cell Delivery","volume":"7","author":"Chen","year":"2025","journal-title":"Adv. Fiber Mater."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Du, D., Asaoka, T., Ushida, T., and Furukawa, K.S. (2014). Fabrication and perfusion culture of anatomically shaped artificial bone using stereolithography. Biofabrication, 6.","DOI":"10.1088\/1758-5082\/6\/4\/045002"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2041731418776819","DOI":"10.1177\/2041731418776819","article-title":"Bone substitutes: A review of their characteristics, clinical use, and perspectives for large bone defects management","volume":"9","author":"Keller","year":"2018","journal-title":"J. Tissue Eng."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actbio.2017.08.030","article-title":"Regenerating bone with bioactive glass scaffolds: A review of in vivo studies in bone defect models","volume":"62","author":"Roether","year":"2017","journal-title":"Acta Biomater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1007\/s10856-006-0432-z","article-title":"The story of Bioglass\u00ae","volume":"17","author":"Hench","year":"2006","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_6","first-page":"P79","article-title":"Bioglass\u00ae stimulation of embryonic long-bones in altered loading environments","volume":"8","author":"Maroothynaden","year":"2001","journal-title":"J. Gravit. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1097\/00003086-199701000-00041","article-title":"Particulate bioglass compared with hydroxyapatite as a bone graft substitute","volume":"334","author":"Oonishi","year":"1997","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3867","DOI":"10.3390\/ma3073867","article-title":"Bioactive Glass and Glass-Ceramic Scaffolds for Bone Tissue Engineering","volume":"3","author":"Gerhardt","year":"2010","journal-title":"Materials"},{"key":"ref_9","unstructured":"Bronzino, J.D., and Peterson, D.R. (2000). The Biomedical Engineering Handbook, CRC Press LLC. [2nd ed.]."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sergi, R., Bellucci, D., and Cannillo, V. (2020). A Comprehensive Review of Bioactive Glass Coatings: State of the Art, Challenges and Future Perspectives. Coatings, 10.","DOI":"10.3390\/coatings10080757"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.actbio.2024.04.005","article-title":"Enriched mesoporous bioactive glass scaffolds as bone substitutes in critical diaphyseal bone defects in rabbits","volume":"180","author":"Lozano","year":"2024","journal-title":"Acta Biomater."},{"key":"ref_12","first-page":"02447","article-title":"Revolutionizing bone regeneration: 3D printing of PLA\/MFBG composites with advanced healing properties","volume":"43","author":"Bernardo","year":"2025","journal-title":"Mater. Today Chem."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Garimella, A., Ghosh, S.B., and Bandyopadhyay-Ghosh, S. (2024). Biomaterials for bone tissue engineering: Achievements to date and future directions. Biomed. Mater., 20.","DOI":"10.1088\/1748-605X\/ad967c"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"413","DOI":"10.5194\/jbji-6-413-2021","article-title":"Mid-term clinical results of chronic cavitary long bone osteomyelitis treatment using S53P4 bioactive glass: A multi-center study","volume":"6","author":"Heidotting","year":"2021","journal-title":"J. Bone Jt. Infect."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1530\/EOR-23-0031","article-title":"Staphylococcus aureus colonization and periprosthetic joint infection in patients undergoing elective total joint arthroplasty: A narrative review","volume":"8","author":"Moodley","year":"2023","journal-title":"EFORT Open Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/ijag.12252","article-title":"Bioglass and bioactive glasses and their impact on healthcare","volume":"7","author":"Jones","year":"2016","journal-title":"Int. J. Appl. Glass Sci."},{"key":"ref_17","first-page":"261","article-title":"Bioactive glass coatings on metallic implants for biomedical applications","volume":"4","author":"Oliver","year":"2019","journal-title":"Bioact. Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.actbio.2024.06.012","article-title":"Emerging materials and technologies for advancing bioresorbable surgical meshes","volume":"184","author":"Heidari","year":"2024","journal-title":"Acta Biomater."},{"key":"ref_19","unstructured":"(2023). Biological Evaluation of Medical Devices\u2014Part 17: Toxicological Risk Assessment of Medical Device Constituents (Standard No. ISO 10993-17:2023)."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Thangaraju, P., and Varthya, S.B. (2022). ISO 10993: Biological evaluation of medical devices. Medical Device Guidelines and Regulations Handbook, Springer International Publishing.","DOI":"10.1007\/978-3-030-91855-2_11"},{"key":"ref_21","unstructured":"U.S. Food and Drug Administration (2025, July 28). Use of International Standard ISO 10993-1: Biological Evaluation of Medical Devices\u2014Part 1: Evaluation and Testing Within a Risk Management Process, Available online: https:\/\/www.fda.gov\/media\/142959\/download."},{"key":"ref_22","unstructured":"Anusavice, K.J. (1996). Phillips\u2019 Science of Dental Materials, WB Saunders. [10th ed.]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1002\/cbin.11073","article-title":"Phenotypic stability of the human MG-63 osteoblastic cell line at different passages","volume":"43","author":"Staehlke","year":"2019","journal-title":"Cell Biol. Int."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"43678","DOI":"10.1021\/acsomega.4c06020","article-title":"Bioresorption and Biomineralization of S53P4 Bioactive Glass in Neutral Tris Buffer and Citric Acid Solution","volume":"9","author":"Yoo","year":"2024","journal-title":"ACS Omega"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.actbio.2018.05.019","article-title":"Tackling bioactive glass excessive in vitro bioreactivity: Preconditioning approaches for cell culture tests","volume":"75","author":"Ciraldo","year":"2018","journal-title":"Acta Biomater."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.acthis.2018.03.007","article-title":"Current methodology of MTT assay in bacteria\u2014A review","volume":"120","author":"Grela","year":"2018","journal-title":"Acta Histochem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.apsusc.2016.02.202","article-title":"Adhesion and inactivation of Gram-negative and Gram-positive bacteria on photoreactive TiO2\/polymer and Ag\u2013TiO2\/polymer nanohybrid films","volume":"371","author":"Nagy","year":"2016","journal-title":"Appl. Surf. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Venkei, A., Ungv\u00e1ri, K., E\u00f6rdegh, G., Janov\u00e1k, L., Urb\u00e1n, E., and Turz\u00f3, K. (2020). Photocatalytic enhancement of antibacterial effects of photoreactive nanohybrid films in an in vitro Streptococcus mitis model. Arch. Oral Biol., 117.","DOI":"10.1016\/j.archoralbio.2020.104837"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"11155","DOI":"10.1007\/s11356-014-2568-6","article-title":"Investigation of the antibacterial effects of silver-modified TiO2 and ZnO plasmonic photocatalysts embedded in polymer thin films","volume":"21","author":"Nagy","year":"2014","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1002\/(SICI)1097-4636(19971205)37:3<394::AID-JBM10>3.0.CO;2-C","article-title":"Human osteoblast-like cells (MG63) proliferate on abioactive glass surface","volume":"37","author":"Price","year":"1997","journal-title":"J. Biomed. Mater. Res."},{"key":"ref_31","unstructured":"(2001). Biological Evaluation of Medical Devices\u2013Part 14: Identification and Quantification of Degradation Products from Ceramics (Standard No. ISO 10993-14:2001)."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.actbio.2022.09.052","article-title":"Engineered Biomaterials to Guide Spheroid Formation, Function, and Fabrication into 3D Tissue Constructs","volume":"165","author":"Burdick","year":"2023","journal-title":"Acta Biomater."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1517\/14712598.2012.707181","article-title":"Advances in the formation, use and understanding of multi-cellular spheroids","volume":"12","author":"Achilli","year":"2012","journal-title":"Expert Opin. Biol. Ther."},{"key":"ref_34","first-page":"148","article-title":"MG-63 human osteosarcoma cells grown in monolayer and as three-dimensional tumor spheroids present a different metabolic profile","volume":"557","author":"Santini","year":"2004","journal-title":"NMR Study"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s13046-017-0663-5","article-title":"Relevance of 3d culture systems to study osteosarcoma environment","volume":"37","author":"Raimondi","year":"2018","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/s41419-021-03890-7","article-title":"Comprehensive understanding of anchorage-independent survival and its implication in cancer metastasis","volume":"12","author":"Deng","year":"2021","journal-title":"Cell Death Dis."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/16\/8\/275\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:18:15Z","timestamp":1760033895000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/16\/8\/275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,29]]},"references-count":36,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["jfb16080275"],"URL":"https:\/\/doi.org\/10.3390\/jfb16080275","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,29]]}}}