{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:17:40Z","timestamp":1791937060395,"version":"4.3.4"},"reference-count":65,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,31]],"date-time":"2025-08-31T00:00:00Z","timestamp":1756598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>This study presents the development of a degradable and biocompatible calcium phosphate cement (CPC) co-loaded with gentamicin (1.25 wt%) and vancomycin (4.25 wt%) for the local treatment of polymicrobial bone infections. The antibiotics were incorporated\u2014individually or in combination\u2014into the solid phase of Graftys\u00ae Quickset (GQS), an injectable CPC. Antibiotic loading modifies some of the intrinsic properties of the GQS cement. Porosity exceeded 53%, compressive strength reduced around 5 MPa, which is comparable to calcium sulphates cements, and the setting time, although extended, remained within the clinically acceptable threshold (&lt;20 min), ensuring suitable handling. A burst release of both antibiotics was observed within the first 24 h, with sustained release over time and no cytotoxic effects on human osteoblasts. The dual-loaded cement exhibited broad-spectrum antibacterial activity against both Gram-positive and Gram-negative strains, including methicillin-resistant isolates, in both planktonic and biofilm forms. Notably, the combination of both antibiotics demonstrated superior efficacy compared to either antibiotic alone. These findings suggest that this dual-antibiotic-loaded CPC offers a promising strategy for localised treatment of complex bone infections such as osteomyelitis, where polymicrobial involvement and antibiotic resistance pose significant therapeutic challenges.<\/jats:p>","DOI":"10.3390\/jfb16090320","type":"journal-article","created":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T16:39:01Z","timestamp":1756744741000},"page":"320","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Antibiofilm Activity of a Novel Calcium Phosphate Cement Doped with Two Antibiotics"],"prefix":"10.3390","volume":"16","author":[{"given":"Eneko","family":"Elezgaray","sequence":"first","affiliation":[{"name":"Institut Europ\u00e9en des Membranes, IEM, UMR 5635, University of Montpellier, Ecole Nationale Sup\u00e9rieure de Chimie de Montpellier, ENSCM, Centre National de la Recherche Scientifique (CNRS), 34095 Montpellier, France"},{"name":"IRCM, Institut de Recherche en Canc\u00e9rologie de Montpellier, INSERM U1194, Universit\u00e9 Montpellier, 34000 Montpellier, France"},{"name":"NORAKER SAS, 60 Av. Rockefeller, 69008 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra","family":"Pouget","sequence":"additional","affiliation":[{"name":"VBIC, INSERM U1047, University of Montpellier, Service de Microbiologie et Hygi\u00e8ne Hospitali\u00e8re, CHU N\u00eemes, 30029 N\u00eemes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanny","family":"Salmeron","sequence":"additional","affiliation":[{"name":"IRCM, Institut de Recherche en Canc\u00e9rologie de Montpellier, INSERM U1194, Universit\u00e9 Montpellier, 34000 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Flacard","sequence":"additional","affiliation":[{"name":"NORAKER SAS, 60 Av. Rockefeller, 69008 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9484-0304","authenticated-orcid":false,"given":"Jean-Philippe","family":"Lavigne","sequence":"additional","affiliation":[{"name":"VBIC, INSERM U1047, University of Montpellier, Service de Microbiologie et Hygi\u00e8ne Hospitali\u00e8re, CHU N\u00eemes, 30029 N\u00eemes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7160-3074","authenticated-orcid":false,"given":"Vincent","family":"Cavaill\u00e8s","sequence":"additional","affiliation":[{"name":"IRCM, Institut de Recherche en Canc\u00e9rologie de Montpellier, INSERM U1194, Universit\u00e9 Montpellier, 34000 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2913-2846","authenticated-orcid":false,"given":"Mikhael","family":"Bechelany","sequence":"additional","affiliation":[{"name":"Institut Europ\u00e9en des Membranes, IEM, UMR 5635, University of Montpellier, Ecole Nationale Sup\u00e9rieure de Chimie de Montpellier, ENSCM, Centre National de la Recherche Scientifique (CNRS), 34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/j.bjid.2013.12.005","article-title":"Recommendations for the treatment of osteomyelitis","volume":"18","author":"Lima","year":"2014","journal-title":"Braz. 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