{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:40:50Z","timestamp":1785436850268,"version":"3.56.0"},"reference-count":27,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T00:00:00Z","timestamp":1761264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["ZIU-MP-14\/09.08"],"award-info":[{"award-number":["ZIU-MP-14\/09.08"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Strategic Research and Innovation Program for the Development of MU-Sofia","award":["BG-RRP-2.004-0004-C01"],"award-info":[{"award-number":["BG-RRP-2.004-0004-C01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Background: This study aims to evaluate the effect of different types of platelet concentrates (autologous blood biomaterials) on the migration potential of human dental pulp stem (hDPSCs). Materials and Methods: Our team created a model of human dental pulp stem cells (hDPSCs). Various types of AB biomaterials were produced from blood samples from volunteers using the protocols presented: A-PRF+, Gel A-PRF+, and Solid PRF. The scratch wound healing assay was used to examine the closure of the experimental wounds on day 1 and day 14. The wound areas were quantified using Image J software. Statistical analysis was performed with the Kruskal\u2013Wallis and Mann\u2013Whitney U tests, as the data did not follow a normal distribution, which was confirmed by the Shapiro\u2013Wilk test (p &lt; 0.05). Results: The results demonstrate significantly faster closure of the experimental wounds on day 14 of the studied biomaterials AB: A-PRF+, Gel A-PRF+, and Solid PRF compared to the control group of cells. Gel A-PRF+ exhibited the most pronounced stimulatory effect on cell migration (p = 0.0036 vs. control), followed by Solid PRF and A-PRF+. Conclusions: The results indicate that autologous blood platelet concentrates stimulate the migration of hDPSCs in vitro. Gel A-PRF+ demonstrated the strongest effect, underscoring its potential clinical relevance for applications in tissue engineering.<\/jats:p>","DOI":"10.3390\/jfb16110398","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T02:23:28Z","timestamp":1761704608000},"page":"398","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Influence of Different Biomaterials Extracted from Autologous Blood on the Cell Migration of Stem Cells from Dental Pulp"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0185-9912","authenticated-orcid":false,"given":"Janet N.","family":"Kirilova","sequence":"first","affiliation":[{"name":"Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3430-4195","authenticated-orcid":false,"given":"Rositsa Z.","family":"Vladova","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0602-5383","authenticated-orcid":false,"given":"Viktoria P.","family":"Petrova","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2698-4189","authenticated-orcid":false,"given":"Sevda","family":"Yantcheva","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry, Faculty of Dental Medicine, Medical University, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2356-9932","authenticated-orcid":false,"given":"Elitsa G.","family":"Deliverska","sequence":"additional","affiliation":[{"name":"Department of Dental, Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Medical University, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4351-5579","authenticated-orcid":false,"given":"Nikolay D.","family":"Ishkitiev","sequence":"additional","affiliation":[{"name":"Department of Medical Chemistry and Biochemistry, Faculty of Medicine, Medical University, 1000 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1177\/0022034511405385","article-title":"Pulp healing and regeneration: More questions than answers","volume":"23","author":"Goldberg","year":"2011","journal-title":"Adv. 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