{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:44:52Z","timestamp":1788320692352,"version":"build-2803163510"},"reference-count":77,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>The key to proper implant integration in bone replacement is to orchestrate the complex interactions between materials and tissues. Moreover, due to the rapid demographic shift towards aging societies and the increase in elderly and osteoporotic patients, it is of great importance that implant materials are osteointegrative in not only healthy but also compromised bone tissues. Here, titanium (Ti) scaffolds were subjected to shifted laser surface texturing (sLST) using a nanosecond pulsed laser to create an open pore macrotopography with micro-and nano-Ti droplets. In contrast to conventional laser texturing, which leads to high heat accumulation; in sLST, the frequency of laser pulses is low, allowing for resolidification, thereby creating a surface with abundant coverage micro-\/nanodroplets. The main objective was to compare the cellular responses of human mesenchymal stromal cells (hMSCs) on sLST-textured Ti surfaces (LT-Ti) for the first time with standard sand-blasted, acid-etched surfaces (SLA-Ti). In-depth analyses of cell survival, proliferation, shape, mineralization, and gene expression were performed. Cell survival\/proliferation was found to be similar on both surfaces; however, SEM imaging revealed differences in hMSC morphology. On LT-Ti, cells adopted well-rounded shapes, whereas on SLA-Ti they assumed more planar shapes. Bulk RNA sequencing performed after short-term culture on both surfaces disclosed expression changes in genes such as DUSP6, TNFSF12-TNFSF13 and SULT1A4. Remarkably, the osteogenic differentiation capacity of hMSCs was significantly enhanced on LT-Ti compared to SLA-Ti. Furthermore, aged\/osteoporotic donor cohorts showed significantly enhanced matrix mineralization on LT-Ti. In conclusion, our novel results demonstrate that sLST-Ti surfaces are safe, highly biocompatible, can rescue patient-cohort-specific mineralization behavior, and therefore hold great potential for the development into next-generation implants, which are suitable for both the elderly and bone-compromised populations.<\/jats:p>","DOI":"10.3390\/jfb16110401","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T02:23:28Z","timestamp":1761704608000},"page":"401","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Micropatterning and Nanodropletting of Titanium by Shifted Surface Laser Texturing Significantly Enhances In Vitro Osteogenesis of Healthy and Osteoporotic Mesenchymal Stromal Cells"],"prefix":"10.3390","volume":"16","author":[{"given":"Theresia","family":"Stich","sequence":"first","affiliation":[{"name":"Experimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, 93055 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Alagboso","sequence":"additional","affiliation":[{"name":"Experimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, 93055 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7647-3769","authenticated-orcid":false,"given":"Girish","family":"Pattappa","sequence":"additional","affiliation":[{"name":"Experimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, 93055 Regensburg, Germany"},{"name":"Department of Musculoskeletal Tissue Regeneration and Department of Orthopedic Surgery, Orthopedic Hospital K\u00f6nig-Ludwig-Haus, Julius-Maximilians-University W\u00fcrzburg, 97074 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Spinal Surgery, The Second Hospital of Dalian Medical University, Dalian Medical University, Dalian 116023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3919-8651","authenticated-orcid":false,"given":"Denys","family":"Moskal","sequence":"additional","affiliation":[{"name":"New Technologies Research Centre, University of West Bohemia, 30100 Pilsen, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6814-7066","authenticated-orcid":false,"given":"Michal","family":"Povoln\u00fd","sequence":"additional","affiliation":[{"name":"Department of Machining Technology, Faculty of Mechanical Engineering, University of West Bohemia, 30100 Pilsen, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8774-7892","authenticated-orcid":false,"given":"Maximilian","family":"Saller","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Trauma Surgery, Musculoskeletal University Centre Munich (MUM), Ludwig-Maximilians-University (LMU) Hospital, 80336 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Sch\u00f6nitzer","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Trauma Surgery, Musculoskeletal University Centre Munich (MUM), Ludwig-Maximilians-University (LMU) Hospital, 80336 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4450-4238","authenticated-orcid":false,"given":"Konstantin J.","family":"Scholz","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, 93055 Regensburg, Germany"},{"name":"Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Cieplik","sequence":"additional","affiliation":[{"name":"Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0208-4650","authenticated-orcid":false,"given":"Volker","family":"Alt","sequence":"additional","affiliation":[{"name":"Experimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, 93055 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4982-6594","authenticated-orcid":false,"given":"Maximilian","family":"Rudert","sequence":"additional","affiliation":[{"name":"Department of Musculoskeletal Tissue Regeneration and Department of Orthopedic Surgery, Orthopedic Hospital K\u00f6nig-Ludwig-Haus, Julius-Maximilians-University W\u00fcrzburg, 97074 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4838-4069","authenticated-orcid":false,"given":"Tom\u00e1\u0161","family":"Kov\u00e1\u0159\u00edk","sequence":"additional","affiliation":[{"name":"New Technologies Research Centre, University of West Bohemia, 30100 Pilsen, Czech Republic"},{"name":"Department of Material Science and Metallurgy, University of West Bohemia, 30100 Pilsen, Czech 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