{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:08:08Z","timestamp":1787062088036,"version":"build-2736575974"},"reference-count":103,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T00:00:00Z","timestamp":1770681600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Context: The increasing incidence of secondary caries and the failure of restorations have intensified research into dental restorative materials capable of actively interacting with the oral environment. In this context, antibacterial and bioregenerative nanomaterials have attracted growing scientific interest due to their potential to inhibit biofilm formation while simultaneously supporting mineral repair processes. Objective: This narrative review analyzes recent developments in nanostructured materials for restorative dentistry and oral health applications, with particular emphasis on antibacterial agents, bioactive systems, and emerging dual-function approaches that integrate multiple biological functions into restorative materials. Scope of the Review: The analyzed literature indicates that metallic nanoparticles, cationic monomers, and natural nanopolymers can reduce bacterial adhesion and metabolic activity under experimental conditions. In parallel, bioactive nanomaterials such as nanohydroxyapatite, bioactive glass, and calcium phosphate-based systems have demonstrated the ability to release remineralizing ions and to promote mineral deposition at the tooth\u2013material interface. Dual-function hybrid materials aim to combine these antibacterial and bioregenerative effects within a single restorative system. Interpretative Perspective: Despite these advances, most available evidence derives from in vitro and preclinical studies, with significant heterogeneity across experimental models, evaluation methods, and outcome variables. This variability limits direct comparisons between studies and necessitates a cautious interpretation of claims regarding long-term antibacterial efficacy, functional tissue regeneration, and routine clinical applicability. Conclusions: Antibacterial and bioregenerative nanomaterials represent a relevant and continuously evolving research direction in restorative dentistry. Their successful clinical translation will depend on establishing standardized testing protocols, conducting comprehensive safety assessments, and generating clinically relevant evidence supporting long-term efficacy and biological compatibility. Their successful clinical translation will depend on establishing standardized testing protocols, conducting comprehensive safety assessments, and generating clinically relevant evidence supporting long-term efficacy and biological compatibility.<\/jats:p>","DOI":"10.3390\/jfb17020087","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T09:16:08Z","timestamp":1770801368000},"page":"87","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antibacterial and Bioregenerative Nanomaterials in Oral Health: From Material Design to Clinical Translation and Technological Trends"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3959-9901","authenticated-orcid":false,"given":"Dana Emanuela","family":"Pitic (Cot)","sequence":"first","affiliation":[{"name":"Management and Communication in Dental Medicine Department I, Faculty of Dental Medicine, Victor Babes University of Medicine and Pharmacy, Pia\u021ba Eftimie Murgu 2, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aniela-Roxana","family":"Nodi\u021bi-Cuc","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, \u2018Carol Davila\u2019 University of Medicine and Pharmacy, 020021 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Ioana","family":"Talpos-Niculescu","sequence":"additional","affiliation":[{"name":"Management and Communication in Dental Medicine Department I, Faculty of Dental Medicine, Victor Babes University of Medicine and Pharmacy, Pia\u021ba Eftimie Murgu 2, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2585-2632","authenticated-orcid":false,"given":"Diana","family":"Marian","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Faculty of Dentistry, \u201cVasile Goldi\u0219\u201d Western University of Arad, 94-96 Revolutiei Blvd., 310025 Arad, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3852-9060","authenticated-orcid":false,"given":"Ramona Amina","family":"Popovici","sequence":"additional","affiliation":[{"name":"Management and Communication in Dental Medicine Department I, Faculty of Dental Medicine, Victor Babes University of Medicine and Pharmacy, Pia\u021ba Eftimie Murgu 2, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4581-8487","authenticated-orcid":false,"given":"Andreea Mihaela","family":"Kis","sequence":"additional","affiliation":[{"name":"Management and Communication in Dental Medicine Department I, Faculty of Dental Medicine, Victor Babes University of Medicine and Pharmacy, Pia\u021ba Eftimie Murgu 2, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laria-Maria","family":"Trusculescu","sequence":"additional","affiliation":[{"name":"Management and Communication in Dental Medicine Department I, Faculty of Dental Medicine, Victor Babes University of Medicine and Pharmacy, Pia\u021ba Eftimie Murgu 2, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adina","family":"Feher","sequence":"additional","affiliation":[{"name":"Management and Communication in Dental Medicine Department I, Faculty of Dental Medicine, Victor Babes University of Medicine and Pharmacy, Pia\u021ba Eftimie Murgu 2, 300041 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6347-3497","authenticated-orcid":false,"given":"Ioana Elena","family":"Lile","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Faculty of Dentistry, \u201cVasile Goldi\u0219\u201d Western University of Arad, 94-96 Revolutiei Blvd., 310025 Arad, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17030","DOI":"10.1038\/nrdp.2017.30","article-title":"Dental caries","volume":"3","author":"Pitts","year":"2017","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0140-6736(07)60031-2","article-title":"Dental caries","volume":"369","author":"Selwitz","year":"2007","journal-title":"Lancet"},{"key":"ref_3","unstructured":"Rathee, M., and Sapra, A. (2025, November 30). Dental Caries, StatPearls, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK551699\/."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1038\/s41415-021-3775-4","article-title":"Understanding dental caries as a non-communicable disease","volume":"231","author":"Pitts","year":"2021","journal-title":"Br. Dent. J."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Giacaman, R.A., Fern\u00e1ndez, C.E., Mu\u00f1oz-Sandoval, C., Le\u00f3n, S., Garc\u00eda-Manr\u00edquez, N., Echeverr\u00eda, C., Vald\u00e9s, S., Castro, R.J., and Gambetta-Tessini, K. (2022). Understanding dental caries as a non-communicable and behavioral disease: Management implications. Front. Oral Health, 3.","DOI":"10.3389\/froh.2022.764479"},{"key":"ref_6","unstructured":"FDI World Dental Federation (2016). White Paper on Dental Caries Prevention and Management, FDI. Available online: https:\/\/www.fdiworlddental.org\/resources\/white-papers\/white-paper-on-dental-caries-prevention-and-management."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.dental.2010.10.020","article-title":"Resin composite\u2014State of the art","volume":"27","author":"Ferracane","year":"2011","journal-title":"Dent. Mater."},{"key":"ref_8","first-page":"367","article-title":"Developments in resin-based composites","volume":"233","author":"German","year":"2022","journal-title":"Br. Dent. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.identj.2022.11.012","article-title":"Bioactivity of Dental Restorative Materials: FDI Policy Statement","volume":"73","author":"Schmalz","year":"2023","journal-title":"Int. Dent. J."},{"key":"ref_10","first-page":"100022","article-title":"Bioactive dental materials Developing, promising, confusing","volume":"2","author":"Ferracane","year":"2023","journal-title":"JADA Found. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1002\/wnan.1224","article-title":"Dental applications of nanostructured bioactive glass and its composites","volume":"5","author":"Polini","year":"2013","journal-title":"Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"025037","DOI":"10.1088\/1758-5090\/aa6ed6","article-title":"3D-printed bioceramic scaffolds with antibacterial and osteogenic activity","volume":"9","author":"Zhang","year":"2017","journal-title":"Biofabrication"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"105600","DOI":"10.1016\/j.jdent.2025.105600","article-title":"Ion-releasing resin composites prevent demineralization around restorations in an in-vitro biofilm model","volume":"139","author":"Ionescu","year":"2025","journal-title":"J. Dent."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1016\/j.dental.2025.07.009","article-title":"Ion-releasing restorative materials and dentin remineralization: Current strategies and future perspectives","volume":"41","author":"Vilela","year":"2025","journal-title":"Dent. Mater."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4378","DOI":"10.1039\/D0RA00762E","article-title":"Applications of nano-materials in dentistry in diverse dentistry regimes","volume":"10","author":"Foong","year":"2020","journal-title":"RSC Adv."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Sreenivasalu, P.K.P., Dora, C.P., Swami, R., Jasthi, V.C., Shiroorkar, P.N., Nagaraja, S., Asdaq, S.M.B., and Anwer, K. (2022). Nanomaterials in Dentistry: Current Applications and Future Scope. Nanomaterials, 12.","DOI":"10.3390\/nano12101676"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/17435390.2012.742935","article-title":"The antibacterial effects of silver, titanium dioxide and silica dioxide nanoparticles compared to the dental disinfectant chlorhexidine on Streptococcus mutans using a suite of bioassays","volume":"8","author":"Besinis","year":"2014","journal-title":"Nanotoxicology"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Abozaid, D., Azab, A., Bahnsawy, M.A., Eldebawy, M., Ayad, A., Soomro, R., Elwakeel, E., and Mohamed, M.A. (2025). Bioactive restorative materials in dentistry: A comprehensive review of mechanisms, clinical applications, and future directions. Odontology.","DOI":"10.1007\/s10266-025-01162-w"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/j.dental.2018.03.014","article-title":"Antibacterial quaternary ammonium compounds in dental materials: A systematic review","volume":"34","author":"Makvandi","year":"2018","journal-title":"Dent. Mater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/j.dental.2023.10.018","article-title":"5-year clinical performance of posterior bulk-filled resin composite restorations: A double-blind randomized controlled trial","volume":"39","author":"Loguercio","year":"2023","journal-title":"Dent. Mater."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Yu, K., Zhang, Q., Dai, Z., Zhu, M., Xiao, L., Zhao, Z., Bai, Y., and Zhang, K. (2023). Smart Dental Materials Intelligently Responding to Oral pH to Combat Caries: A Literature Review. Polymers, 15.","DOI":"10.3390\/polym15122611"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Cicci\u00f9, M., Fiorillo, L., and Cervino, G. (2019). Chitosan Use in Dentistry: A Systematic Review of Recent Clinical Studies. Mar. Drugs, 17.","DOI":"10.3390\/md17070417"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Alhussein, A., Alsahafi, R., Alfaifi, A., Alenizy, M., Ba-Armah, I., Schneider, A., Jabra-Rizk, M.-A., Masri, R., Garcia Fay, G., and Oates, T.W. (2023). Novel remineralizing and antibiofilm low-shrinkage-stress nanocomposites to inhibit salivary biofilms and protect tooth structures. Materials, 16.","DOI":"10.3390\/ma16206770"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sun, Q., Zhang, L., Bai, R., Zhuang, Z., Zhang, Y., Yu, T., Peng, L., Xin, T., Chen, S., and Han, B. (2021). Recent progress in antimicrobial strategies for resin-based restoratives. Polymers, 13.","DOI":"10.3390\/polym13101590"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Degli Esposti, L., Ionescu, A.C., Carella, F., Adamiano, A., Brambilla, E., and Iafisco, M. (2022). Antimicrobial activity of remineralizing ion-doped amorphous calcium phosphates for preventive dentistry. Front. Mater., 9.","DOI":"10.3389\/fmats.2022.846130"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Rodr\u00edguez-G\u00f3mez, F.D., Monferrer, D., Penon, O., and Rivera-Gil, P. (2025). Regulatory pathways and guidelines for nanotechnology-enabled health products: A comparative review of EU and US frameworks. Front. Med., 12.","DOI":"10.3389\/fmed.2025.1544393"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1515\/jmbm-2025-0071","article-title":"Mechanical and antibacterial characteristics of a 3D-printed nano-titanium dioxide-hydroxyapatite dental resin-based composite","volume":"34","author":"Rohmadi","year":"2025","journal-title":"J. Mech. Behav. Mater."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Najeeb, M., and Islam, S. (2025). Artificial intelligence (AI) in restorative dentistry: Current trends and future prospects. BMC Oral Health, 25.","DOI":"10.1186\/s12903-025-05989-1"},{"key":"ref_29","first-page":"100005","article-title":"Pioneering the future of dentistry: AI-driven 3D bioprinting for next-generation clinical applications","volume":"1","author":"Liang","year":"2025","journal-title":"Transl. Dent. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Marta, M.-M., Chivu, O.R., Marian, D., Enache, I.-C., Veja, I., Pitic, D.E., Flueras, R., Popovici, R.A., Stana, A.H., and Cojocariu, C. (2024). Elemental composition and dentin bioactivity at the interface with AH Plus bioceramic sealer: An energy-dispersive X-ray spectroscopy study. Appl. Sci., 14.","DOI":"10.3390\/app142411867"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Marian, D., Popovici, R.A., Olariu, I., Pitic, D.E., Marta, M.-M., and Veja, I. (2025). Patterns and Practices in the Use of Endodontic Materials: Insights from Romanian Dental Practices. Appl. Sci., 15.","DOI":"10.3390\/app15031272"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1016\/j.dental.2015.03.011","article-title":"Cyclic mechanical loading promotes bacterial penetration along composite restoration marginal gaps","volume":"31","author":"Khvostenko","year":"2015","journal-title":"Dent. Mater."},{"key":"ref_33","first-page":"364","article-title":"Models of caries formation around dental composite restorations","volume":"95","author":"Ferracane","year":"2016","journal-title":"J. Dent. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1177\/0022034510379602","article-title":"The role of bacteria in the caries process: Ecological perspectives","volume":"90","author":"Takahashi","year":"2011","journal-title":"J. Dent. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Mo, S., Bao, W., Lai, G.-Y., Wang, J., and Li, M.-Y. (2010). The microfloral analysis of secondary caries biofilm around Class I and Class II composite and amalgam fillings. BMC Infect. Dis., 10.","DOI":"10.1186\/1471-2334-10-241"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Li, X., Liang, X., Wang, Y., Wang, D., Teng, M., Xu, H., Zhao, B., and Han, L. (2022). Graphene-Based Nanomaterials for Dental Applications: Principles, Current Advances, and Future Outlook. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.804201"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"100635","DOI":"10.1016\/j.mtbio.2023.100635","article-title":"Recent advances on nanomaterials for antibacterial treatment of oral diseases","volume":"20","author":"Chen","year":"2023","journal-title":"Mater. Today Bio"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"9322","DOI":"10.1021\/am402618w","article-title":"Role of size and shape on biofilm eradication for nitric oxide-releasing silica nanoparticles","volume":"5","author":"Slomberg","year":"2013","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.2147\/IJN.S557649","article-title":"Antimicrobial mechanisms of metal-based nanomaterials","volume":"20","author":"Liao","year":"2025","journal-title":"Int. J. Nanomed."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4764","DOI":"10.1039\/D0TB00099J","article-title":"Advances and challenges in metallic nanomaterial synthesis and antibacterial applications","volume":"8","author":"Guo","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2555","DOI":"10.2147\/IJN.S246764","article-title":"The antibacterial mechanism of silver nanoparticles and its application in dentistry","volume":"15","author":"Yin","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Mallineni, S.K., Sakhamuri, S., Kotha, S.L., AlAsmari, A.R.G.M., AlJefri, G.H., Almotawah, F.N., Mallineni, S., and Sajja, R. (2023). Silver Nanoparticles in Dental Applications: A Descriptive Review. Bioengineering, 10.","DOI":"10.3390\/bioengineering10030327"},{"key":"ref_43","first-page":"485275","article-title":"Silver nanoparticles in dental biomaterials","volume":"2015","author":"Mori","year":"2015","journal-title":"Int. J. Biomater."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Syci\u0144ska-Dziarnowska, M., Szyszka-Sommerfeld, L., Wo\u017aniak, K., and Spagnuolo, G. (2023). Silver nanoparticles in dentistry: Investigating research prospects for silver-based biomaterials. Eng. Proc., 56.","DOI":"10.3390\/ASEC2023-15911"},{"key":"ref_45","first-page":"18618","article-title":"Biohybrid nanomaterials in biomedicine: Current trends and future prospects","volume":"4","author":"Asadi","year":"2025","journal-title":"Adv. Appl. NanoBio Technol."},{"key":"ref_46","first-page":"e33779","article-title":"Application of nanomaterials in restorative dentistry","volume":"15","author":"Mandhalkar","year":"2023","journal-title":"Cureus"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Bruna, T., Maldonado-Bravo, F., Jara, P., and Caro, N. (2021). Silver nanoparticles and their antibacterial applications. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22137202"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"546","DOI":"10.25258\/ijpqa.15.1.82","article-title":"Silver nanoparticles as antimicrobial agents: Mechanisms, challenges, and applications","volume":"15","author":"Yadav","year":"2024","journal-title":"Int. J. Pharm. Qual. Assur."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"9132555","DOI":"10.1155\/bmri\/9132555","article-title":"Effect of nanohydroxyapatite and silver nanoparticle incorporation on the flexural strength of resin composites","volume":"2025","author":"Moradaian","year":"2025","journal-title":"BioMed Res. Int."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s43939-025-00204-y","article-title":"Enhancing antimicrobial, physical, and mechanical properties of restorative dental materials through metallic nanoparticle incorporation: A systematic review and meta-analysis","volume":"5","author":"Lima","year":"2025","journal-title":"Discov. Mater."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1590\/0103-644020256255","article-title":"Addition of silver nanoparticles to universal adhesive: In vitro effect on biofilm and shear bond strength","volume":"36","author":"Favaro","year":"2025","journal-title":"Braz. Dent. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"240915","DOI":"10.1098\/rsos.240915","article-title":"Silver nanoparticles incorporated dental restorative resin and its antibiofilm effect","volume":"11","author":"Manisekaran","year":"2024","journal-title":"R. Soc. Open Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3181","DOI":"10.2147\/IJN.S253833","article-title":"Use of silver nanomaterials for caries prevention: A concise review","volume":"15","author":"Yin","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"035004","DOI":"10.1088\/1468-6996\/9\/3\/035004","article-title":"Enhanced bioactivity of ZnO nanoparticles\u2014An antimicrobial study","volume":"9","author":"Padmavathy","year":"2008","journal-title":"Sci. Technol. Adv. Mater."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.ijheh.2016.06.003","article-title":"Antimicrobial applications of copper","volume":"219","author":"Vincent","year":"2016","journal-title":"Int. J. Hyg. Environ. Health"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Oetiker, N., Salinas, D., Lucero-Mora, J., Orellana, R., Quiroz-Mu\u00f1oz, M., Bravo, D., and P\u00e9rez-Donoso, J.M. (2024). Antimicrobial effect of copper nanoparticles on relevant supragingival oral bacteria. Microorganisms, 12.","DOI":"10.3390\/microorganisms12030624"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e115","DOI":"10.1016\/j.dental.2016.11.008","article-title":"Silver-doped titanium dioxide nanoparticles as antimicrobial additives to dental polymers","volume":"33","author":"Chambers","year":"2017","journal-title":"Dent. Mater."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"17089","DOI":"10.1021\/acsami.8b04045","article-title":"Metal TiO2 nanotube layers for the treatment of dental implant infections","volume":"10","author":"Roguska","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Song, X., Segura-Egea, J.J., and D\u00edaz-Cuenca, A. (2023). Sol\u2013gel technologies to obtain advanced bioceramics for dental therapeutics. Molecules, 28.","DOI":"10.3390\/molecules28196967"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Preda, S., Pandele-Cu\u0219u, J., Petrescu, S.V., Ciobanu, E.M., Petcu, G., Culi\u021b\u0103, D.C., Apostol, N.G., Costescu, R.M., Raut, I., and Constantin, M. (2022). Photocatalytic and antibacterial properties of doped TiO2 nanopowders synthesized by sol\u2013gel method. Gels, 8.","DOI":"10.3390\/gels8100673"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"e202400807","DOI":"10.1002\/cmdc.202400807","article-title":"Nature-inspired novel quaternary ammonium compounds: Synthesis, antibacterial and antibiofilm activity","volume":"20","author":"Muzychka","year":"2025","journal-title":"ChemMedChem"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Liang, X., Yu, B., Ye, L., Lin, D., Zhang, W., Zhong, H.-J., and He, J. (2024). Recent advances in quaternary ammonium monomers for dental applications. Materials, 17.","DOI":"10.3390\/ma17020345"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Zhang, N., Zhang, K., Xie, X., Dai, Z., Zhao, Z., Imazato, S., Al-Dulaijan, Y.A., Al-Qarni, F.D., Weir, M.D., and Reynolds, M.A. (2018). Nanostructured polymeric materials with protein-repellent and anti-caries properties for dental applications. Nanomaterials, 8.","DOI":"10.3390\/nano8060393"},{"key":"ref_64","first-page":"93","article-title":"Evaluation of antibacterial efficiency of chitosan and chitosan nanoparticles on cariogenic Streptococci: In vitro study","volume":"8","author":"Aliasghari","year":"2016","journal-title":"Iran J. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Dobrzy\u0144ski, W., Piszko, P.J., Kiryk, J., Kiryk, S., Michalak, M., Kotela, A., Kensy, J., \u015awienc, W., Grychowska, N., and Matys, J. (2025). Dental resin composites modified with chitosan: A systematic review. Mar. Drugs, 23.","DOI":"10.3390\/md23050199"},{"key":"ref_66","first-page":"e66296","article-title":"Advanced dental care: \u03b2-Chitosan zinc oxide nanoparticles targeting cariogenic microorganisms","volume":"16","author":"Rajasekar","year":"2024","journal-title":"Cureus"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"111184","DOI":"10.1016\/j.msec.2020.111184","article-title":"Chitosan encapsulated ZnO nanocomposites: Fabrication, characterization, and functionalization of bio-dental approaches","volume":"116","author":"Javed","year":"2020","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1007\/s11845-020-02243-1","article-title":"Antibacterial and antifungal impacts of combined silver, zinc oxide, and chitosan nanoparticles within tissue conditioners of complete dentures in vitro","volume":"189","author":"Mousavi","year":"2020","journal-title":"Ir. J. Med. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"152001","DOI":"10.1088\/1361-6528\/aa6150","article-title":"The graphene oxide contradictory effects against human pathogens","volume":"28","author":"Palmieri","year":"2017","journal-title":"Nanotechnology"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Prodan, D., Moldovan, M., Cuc, S., Sarosi, C., Petean, I., Filip, M., Carpa, R., Doukeh, R., and Mirica, I.C. (2024). Advanced dentistry biomaterials containing graphene oxide. Polymers, 16.","DOI":"10.3390\/polym16121743"},{"key":"ref_71","first-page":"103","article-title":"Applications of graphene oxide and reduced graphene oxide in advanced dental materials and therapies","volume":"19","author":"Sindi","year":"2024","journal-title":"J. Taibah Univ. Med. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.18203\/2320-6012.ijrms20221807","article-title":"Graphene: A game changer in prosthodontics and implant dentistry","volume":"10","author":"Madhav","year":"2022","journal-title":"Int. J. Res. Med. Sci."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"He, Y., Vasilev, K., and Zilm, P. (2023). pH-responsive biomaterials for the treatment of dental caries\u2014A focused and critical review. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15071837"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.dental.2024.09.017","article-title":"Applications of smart materials in minimally invasive dentistry\u2014Some research and clinical perspectives","volume":"40","author":"Par","year":"2024","journal-title":"Dent. Mater."},{"key":"ref_75","unstructured":"(2025). Biological Evaluation of Medical Devices. Part 1: Requirements and General Principles for the Evaluation of Biological Safety Within a Risk Management Process (Standard No. ISO 10993-1:2025)."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"105783","DOI":"10.1016\/j.jmbbm.2023.105783","article-title":"Multifunctional dental resin composite with antibacterial and remineralization properties containing nMgO-BAG","volume":"146","author":"Tian","year":"2023","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Trifan, A., Liciu, E., Busuioc, C., Stancu, I.-C., Banciu, A., Nicolae, C., Dragomir, M., Cristea, D.-D., Sab\u0103u, R.-E., and Ni\u021bulescu, D.-A. (2025). Developing bioengineered 3D-printed composite scaffolds with antimicrobial potential for bone tissue regeneration. J. Funct. Biomater., 16.","DOI":"10.20944\/preprints202505.1535.v1"},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Talaat, S., Hashem, A.A., Abu-Seida, A., Wahed, A.A., and Aziz, T.M.A. (2024). Regenerative potential of mesoporous silica nanoparticles scaffold on dental pulp and root maturation in immature dog\u2019s teeth: A histologic and radiographic study. BMC Oral Health, 24.","DOI":"10.1186\/s12903-024-04368-6"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Muresan, G.C., Boca, S., Lucaciu, O., and Hedesiu, M. (2024). The applicability of nanostructured materials in regenerating soft and bone tissue in the oral cavity\u2014A review. Biomimetics, 9.","DOI":"10.3390\/biomimetics9060348"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Pecci-Lloret, M.P., Gea-Alcocer, S., Murcia-Flores, L., Rodr\u00edguez-Lozano, F.J., and O\u00f1ate-S\u00e1nchez, R.E. (2024). Use of nanoparticles in regenerative dentistry: A systematic review. Biomimetics, 9.","DOI":"10.3390\/biomimetics9040243"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.2147\/IJN.S165080","article-title":"Remineralization potential of nano-hydroxyapatite on enamel and cementum surrounding margin of CAD\/CAM ceramic restoration","volume":"13","author":"Juntavee","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"Muntean, F.L., Olariu, I., Marian, D., Olariu, T., Petrescu, E.L., and Dr\u0103ghici, G.A. (2024). Hydroxyapatite from mollusk shells: Characteristics, production, and potential applications in dentistry. Dent. J., 12.","DOI":"10.3390\/dj12120409"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1007\/s10856-006-0432-z","article-title":"The story of Bioglass","volume":"17","author":"Hench","year":"2006","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1016\/j.msec.2017.03.083","article-title":"Bioactive glass for dentin remineralization: A systematic review","volume":"76","author":"Fernando","year":"2017","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Skallevold, H.E., Rokaya, D., Khurshid, Z., and Zafar, M.S. (2019). Bioactive glass applications in dentistry. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20235960"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1016\/j.dental.2024.08.006","article-title":"Sol\u2013gel-derived calcium silicate cement incorporating collagen and mesoporous bioglass nanoparticles for dental pulp therapy","volume":"40","author":"Simila","year":"2024","journal-title":"Dent. Mater."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1016\/j.dental.2017.06.015","article-title":"Effect of calcium phosphate nanocomposite on in vitro remineralization of human dentin lesions","volume":"33","author":"Weir","year":"2017","journal-title":"Dent. Mater."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.joen.2017.10.012","article-title":"Outcomes of direct pulp capping by using either ProRoot MTA or Biodentine in permanent teeth with carious pulp exposure in 6\u201318-year-old patients: A randomized controlled trial","volume":"44","author":"Parinyaprom","year":"2018","journal-title":"J. Endod."},{"key":"ref_89","first-page":"13","article-title":"Use of vital pulp therapies in primary teeth 2024","volume":"46","author":"Coll","year":"2024","journal-title":"Pediatr. Dent."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s40824-021-00203-z","article-title":"Bioceramic hydroxyapatite-based scaffold with a porous structure using honeycomb as a natural polymeric porogen for bone tissue engineering","volume":"25","author":"Sari","year":"2021","journal-title":"Biomater. Res."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1016\/j.dental.2022.06.017","article-title":"Novel bioactive adhesive containing dimethylaminohexadecyl methacrylate and calcium phosphate nanoparticles to inhibit metalloproteinases and nanoleakage with three months of aging in artificial saliva","volume":"38","author":"Wu","year":"2022","journal-title":"Dent. Mater."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"103344","DOI":"10.1016\/j.jdent.2020.103344","article-title":"Antibacterial and remineralizing nanocomposite inhibits root caries biofilms and protects root dentin hardness at the margins","volume":"99","author":"Zhou","year":"2020","journal-title":"J. Dent."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.21608\/edj.2022.139190.2113","article-title":"Effect of bioactive glass nanoparticles and nano-hydroxyapatite coated by chitosan on odontogenic differentiation and proliferation of human dental pulp stem cells","volume":"68","author":"Ali","year":"2022","journal-title":"Egypt. Dent. J."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Siew Ching, H., Thirumulu Ponnuraj, K., Luddin, N., Ab Rahman, I., and Nik Abdul Ghani, N.R. (2020). Early odontogenic differentiation of dental pulp stem cells treated with nanohydroxyapatite\u2013silica\u2013glass ionomer cement. Polymers, 12.","DOI":"10.3390\/polym12092125"},{"key":"ref_95","first-page":"1043","article-title":"Metal\/metal oxide nanoparticles: Toxicity concerns associated with their physical state and remediation for biomedical applications","volume":"8","author":"Manuja","year":"2021","journal-title":"Toxicol. Rep."},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Zhou, W., Peng, X., Zhou, X., Bonavente, A., Weir, M.D., Melo, M.A.S., Imazato, S., Oates, T.W., Cheng, L., and Xu, H.H.K. (2020). Novel nanocomposite inhibiting caries at the enamel restoration margins in an in vitro saliva-derived biofilm secondary caries model. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176369"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.3390\/polym16142017","article-title":"Polymer-based hydroxyapatite\u2013silver composite resin with enhanced antibacterial activity for dental applications","volume":"16","year":"2024","journal-title":"Polymers"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Torkian, P., Mortazavi Najafabadi, S., Ghashang, M., and Grzelczyk, D. (2023). Glass\u2013ceramic fillers based on zinc oxide\u2013silica systems for dental composite resins: Effect on mechanical properties. Materials, 16.","DOI":"10.3390\/ma16186268"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"11","DOI":"10.5714\/CL.2016.17.1.011","article-title":"A review: Synthesis and applications of graphene\/chitosan nanocomposites","volume":"17","author":"Yuan","year":"2016","journal-title":"Carbon Lett."},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Vasiliu, S., Racovita, S., Gugoasa, I.A., Lungan, M.A., Popa, M., and Desbrieres, J. (2021). The benefits of smart nanoparticles in dental applications. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052585"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"105153","DOI":"10.1016\/j.identj.2025.105153","article-title":"Ai-Optimized 3D-Printed Abutments In Prosthodontics","volume":"75","author":"Wang","year":"2025","journal-title":"Int. Dent. J."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.sdentj.2023.08.013","article-title":"Local and systemic adverse effects of nanoparticles incorporated in dental materials: A critical review","volume":"36","author":"Karunakaran","year":"2024","journal-title":"Saudi Dent. J."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3934\/molsci.2025010","article-title":"Therapeutic application of curcumin and its nanoformulation in dentistry: Opportunities and challenges","volume":"12","author":"Zhang","year":"2025","journal-title":"AIMS Mol. Sci."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/2\/87\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T09:48:29Z","timestamp":1770803309000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/2\/87"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,10]]},"references-count":103,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["jfb17020087"],"URL":"https:\/\/doi.org\/10.3390\/jfb17020087","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,10]]}}}