{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T17:30:54Z","timestamp":1772645454255,"version":"3.50.1"},"reference-count":54,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III (ISCIII)","doi-asserted-by":"publisher","award":["PI22\/01630"],"award-info":[{"award-number":["PI22\/01630"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Commission ERDF\/FEDER Operational Programme of Murcia","award":["50463"],"award-info":[{"award-number":["50463"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Colorectal resection and subsequent anastomosis are the standard curative procedures for a variety of colorectal pathologies. However, anastomotic leakage (AL) is an early and frequent complication that can have life-threatening outcomes. The study aimed to evaluate the effect of silkworm fibroin sheets on colon anastomotic strength and wound healing early after intervention in Wistar rats. Male Wistar rats were randomized into two groups, control (N = 11) and fibroin (N = 11), and subjected to end-to-end colo-colic anastomosis. In the fibroin group, a single layer of fibroin membrane was applied externally around the anastomosis. The animals were sacrificed three days after the operation (POD3) and intestinal adhesions, anastomotic bursting pressure and histological parameters based on the eosin, hematoxylin, and Masson\u2019s trichrome stains were compared between the groups. Fibroin-treated rats showed a significant increase in anastomotic bursting pressure compared to control animals (69 (18) vs. 41 (28) mmHg), whereas no differences in the intestinal adhesion scores were detected. No significant differences in the numbers of granulocytes, monocytes\/macrophages and fibroblasts, nor the amount of collagen fibers, as measured by Masson\u2019s trichrome stain, were found between the groups. These results indicate that fibroin sheets could represent a simple and promising tool to provide mechanical support and improve colonic anastomotic strength early after intervention.<\/jats:p>","DOI":"10.3390\/jfb17030126","type":"journal-article","created":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T13:11:36Z","timestamp":1772629896000},"page":"126","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Silk Fibroin Sheets Improve the Strength of Colon Anastomoses in Wistar Rats"],"prefix":"10.3390","volume":"17","author":[{"given":"Mohamed Hassin","family":"Mohamed Chairi","sequence":"first","affiliation":[{"name":"Servicio de Cirug\u00eda General, Hospital Universitario Virgen de las Nieves, 18012 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3516-2854","authenticated-orcid":false,"given":"Francisco Jos\u00e9","family":"Huertas Pe\u00f1a","sequence":"additional","affiliation":[{"name":"Servicio de Cirug\u00eda General, Hospital Universitario Virgen de las Nieves, 18012 Granada, Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-9441","authenticated-orcid":false,"given":"Jorge","family":"Garc\u00eda-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"},{"name":"Departmento de Farmacologia, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"L\u00f3pez-Esc\u00e1nez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"},{"name":"Departmento de Farmacologia, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9809-7215","authenticated-orcid":false,"given":"Salvador D.","family":"Aznar-Cervantes","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Gen\u00f3mica y Mejora Vegetal, Instituto Murciano de Investigaci\u00f3n y Desarrollo Agrario y Medioambiental (IMIDA), La Alberca, 30150 Murcia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Becerra Massare","sequence":"additional","affiliation":[{"name":"Servicio de Anatom\u00eda Patol\u00f3gica, Hospital 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III e Inmunolog\u00eda, University of Granada, 18016 Granada, Spain"},{"name":"Servicio de An\u00e1lisis Cl\u00ednicos e Inmunolog\u00eda, Hospital Universitario Virgen de las Nieves, 18012 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1554-9226","authenticated-orcid":false,"given":"Jos\u00e9 Alberto","family":"Molina-Tijeras","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"},{"name":"Departmento de Farmacologia, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9085-8147","authenticated-orcid":false,"given":"Mar\u00eda Elena","family":"Rodr\u00edguez-Cabezas","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria de Granada (ibs.GRANADA), 18012 Granada, Spain"},{"name":"Departmento de Farmacologia, Center for Biomedical Research (CIBM), University of Granada, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"2015 European Society of Coloproctology Collaborating Group, Battersby, N., Bhangu, A., Chaudhri, S., El-Hussuna, A., Frasson, M., Nepogodiev, D., Singh, B., Vennix, S., and Zmora, O. 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