{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T09:14:28Z","timestamp":1776417268004,"version":"3.51.2"},"reference-count":79,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T00:00:00Z","timestamp":1776384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>The aim of this study was to evaluate setting time, hardness, solubility, bioactivity and interaction with dentin of four calcium silicate-based sealers (CSBS). Three single-phase CSBS (AH Plus Bioceramic\/AHB, CeraSeal\/CSL, TotalFill BC\/TFL), one powder\/liquid CSBS (BioRoot RCS\/BRT) and an epoxy control (AH Plus Jet\/AHP) were investigated. Setting time was evaluated on glass (G1) and dentin (G2) surfaces, by adding 1%wt purified water to single-phase products. For hardness measurements, the Shore-D hardness test was used. Solubility was assessed according to the ISO 6876:2012 standard. For bioactivity screening, 1-week set specimens were immersed in SBF or water (30 days\/37 \u00b0C) and examined by ATR\u2013FTIR spectroscopy. Interaction with dentin was tested by ATR\u2013FTIR before and after contact with the sealers. For setting time in G1, all CSBS failed to comply with the ISO standard, while in G2, most materials were set in the range of 6\u20138 h, except for CSL. The ranking of significant differences in hardness was AHP, BRT &gt; CSL, AHB, TFL. Regarding solubility, AHB, BRT and AHP were found to comply with the ISO standard, whereas CSL and TFL failed. For bioactivity, characteristic peaks of calcium phosphates were found in all CSBS, with TFL being the most bioactive. A chemical interaction between CSBS and dentin was registered, with a strong reduction in collagen peaks and an increase in carbonates. The CSBS tested exhibited great variance in their behaviour regarding the properties assessed, although a strong deproteinating effect was registered on dentin for all.<\/jats:p>","DOI":"10.3390\/jfb17040192","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T07:48:12Z","timestamp":1776412092000},"page":"192","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Setting Characteristics, Solubility, Bioactivity and Interaction with Dentin of Four Calcium Silicate-Based Endodontic Sealers"],"prefix":"10.3390","volume":"17","author":[{"given":"Areti Dimitra","family":"Vrochari","sequence":"first","affiliation":[{"name":"Department of Endodontics, School of Dentistry, National and Kapodistrian University of Athens, 2 Thivon str, 11527 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anastasia","family":"Agrafioti","sequence":"additional","affiliation":[{"name":"Department of Endodontics, School of Dentistry, National and Kapodistrian University of Athens, 2 Thivon str, 11527 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Dimitriadi","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, School of Dentistry, National and Kapodistrian University of Athens, 2 Thivon Str, 11527 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2210-1075","authenticated-orcid":false,"given":"George","family":"Eliades","sequence":"additional","affiliation":[{"name":"Department of Biomaterials, School of Dentistry, National and Kapodistrian University of Athens, 2 Thivon Str, 11527 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,17]]},"reference":[{"key":"ref_1","first-page":"e120","article-title":"Biological and physico-chemical properties of new root canal sealers","volume":"10","author":"Colombo","year":"2018","journal-title":"J. Clin. Exp. Dent."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"L\u00f3pez-Garc\u00eda, S., Pecci-Lloret, M.R., Guerrero-Giron\u00e9s, J., Pecci-Lloret, M.P., Lozano, A., Llena, C., Rodr\u00edguez-Lozano, F.J., and Forner, L. (2019). Comparative cytocompatibility and mineralization potential of Bio-C Sealer and TotalFill BC Sealer. Materials, 12.","DOI":"10.3390\/ma12193087"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1111\/iej.13086","article-title":"Cytocompatibility, bioactive potential and antimicrobial activity of an experimental calcium silicate-based endodontic sealer","volume":"52","author":"Rodrigues","year":"2019","journal-title":"Int. Endod. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1111\/iej.13327","article-title":"Chemical composition and bioactivity potential of the new Endosequence BC Sealer formulation Hi Flow","volume":"53","author":"Santos","year":"2020","journal-title":"Int. Endod. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.joen.2022.10.009","article-title":"Physicochemical and biological properties of AH Plus Bioceramic","volume":"49","author":"Souza","year":"2023","journal-title":"J. Endod."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Seo, D.G., Lee, D., Kim, Y.M., Song, D., and Kim, S.Y. (2019). Biocompatibility and mineralization activity of three calcium silicate-based root canal sealers compared to conventional resin-based sealer in human dental pulp stem cells. Materials, 12.","DOI":"10.3390\/ma12152482"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1111\/iej.13805","article-title":"Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study","volume":"55","author":"Sanz","year":"2022","journal-title":"Int. Endod. J."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Moraes, T.G., Menezes, A.S., Grazziotin-Soares, R., Moraes, R.U.M.E., Ferreira, P.V.C., Carvalho, C.N., Bauer, J., and Carvalho, E.M. (2022). Impact of immersion media on physical properties and bioactivity of epoxy resin-based and bioceramic endodontic sealers. Polymers, 14.","DOI":"10.3390\/polym14040729"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zamparini, F., Prati, C., Taddei, P., Spinelli, A., Di Foggia, M., and Gandolfi, M.G. (2022). Chemical-physical properties and bioactivity of new premixed calcium silicate-bioceramic root canal sealers. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232213914"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6209","DOI":"10.1007\/s00784-022-04570-2","article-title":"A comprehensive in vitro comparison of the biological and physicochemical properties of bioactive root canal sealers","volume":"26","author":"Wuersching","year":"2022","journal-title":"Clin. Oral Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/j.joen.2016.06.002","article-title":"Properties of tricalcium silicate sealers","volume":"42","author":"Khalil","year":"2016","journal-title":"J. Endod."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1016\/j.joen.2017.07.005","article-title":"Physicochemical properties and volumetric change of silicone\/bioactive glass and calcium silicate-based endodontic sealers","volume":"43","author":"Torres","year":"2017","journal-title":"J. Endod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e42","DOI":"10.5395\/rde.2020.45.e42","article-title":"Flow characteristics and alkalinity of novel bioceramic root canal sealers","volume":"45","author":"Katakidis","year":"2020","journal-title":"Restor. Dent. Endod."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kharouf, N., Arntz, Y., Eid, A., Zghal, J., Sauro, S., Haikel, Y., and Mancino, D. (2020). Physicochemical and antibacterial properties of novel, premixed calcium silicate-based sealer compared to powder\u2013liquid bioceramic sealer. J. Clin. Med., 9.","DOI":"10.3390\/jcm9103096"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1002\/cre2.652","article-title":"Sealing ability in vitro study and biocompatibility in vivo animal study of different bioceramic based sealers","volume":"8","author":"Haji","year":"2022","journal-title":"Clin. Exp. Dent. Res."},{"key":"ref_16","first-page":"2686247","article-title":"Penetration of three endodontic sealers in simulated lateral canals during the lateral condensation technique: An in vitro study","volume":"16","year":"2022","journal-title":"Int. J. Dent."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"774","DOI":"10.1111\/iej.12513","article-title":"Porosity and sealing ability of root fillings with gutta-percha and BioRoot RCS or AH Plus sealers. Evaluation by three ex vivo methods","volume":"49","author":"Viapiana","year":"2016","journal-title":"Int. Endod. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1111\/iej.13744","article-title":"Micro-CT assessment of gap containing areas along the gutta-percha-sealer interface in oval-shaped canals","volume":"55","author":"Santos","year":"2022","journal-title":"Int. Endod. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.jdent.2016.06.008","article-title":"The effect of the final irrigant on the antimicrobial activity of root canal sealers","volume":"52","author":"Camilleri","year":"2016","journal-title":"J. Dent."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/j.joen.2019.04.004","article-title":"The antimicrobial effect of bioceramic sealer on an 8-week matured Enterococcus Faecalis biofilm attached to root canal dentinal surface","volume":"45","author":"Bukhari","year":"2019","journal-title":"J. Endod."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Donnermeyer, D., Schemk\u00e4mper, P., B\u00fcrklein, S., and Sch\u00e4fer, E. (2022). short and long-term solubility, alkalizing effect, and thermal persistence of premixed calcium silicate-based sealers: AH Plus Bioceramic Sealer vs. Total Fill BC Sealer. Materials, 15.","DOI":"10.3390\/ma15207320"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.joen.2011.01.003","article-title":"Setting properties and cytotoxicity evaluation of a premixed bioceramic root canal sealer","volume":"37","author":"Loushine","year":"2011","journal-title":"J. Endod."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1784","DOI":"10.1016\/j.joen.2016.09.018","article-title":"Material properties of a tricalcium silicate\u2013containing, a mineral trioxide aggregate\u2013containing, and an epoxy resin\u2013based root canal sealer","volume":"42","author":"Prullage","year":"2016","journal-title":"J. Endod."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/aej.70044","article-title":"Influence of drying protocol on the setting and crystalline phase formation of calcium silicate-based sealers","volume":"52","author":"Antunes","year":"2025","journal-title":"Aus. Endod. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/j.1365-2591.2011.01992.x","article-title":"Changes in the surface of four calcium silicate-containing endodontic materials and an epoxy resin-based sealer after a solubility test","volume":"45","author":"Borges","year":"2012","journal-title":"Int. Endod. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e120","DOI":"10.1111\/iej.12856","article-title":"Properties of BioRoot RCS, a tricalcium silicate endodontic sealer modified with povidone and polycarboxylate","volume":"50","author":"Siboni","year":"2017","journal-title":"Int. Endod. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e214","DOI":"10.1016\/j.dental.2018.05.011","article-title":"Synthesis of silver-containing calcium aluminate particles and their effects on a MTA-based endodontic sealer","volume":"34","author":"Almeida","year":"2018","journal-title":"Dent. Mater. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/j.joen.2019.01.011","article-title":"Characterization of leachates from 6 root canal sealers","volume":"45","author":"Elyassi","year":"2019","journal-title":"J. Endod."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/iej.13225","article-title":"Effect of immersion in distilled water or phosphate buffered saline on the solubility, volumetric change and presence of voids within new calcium silicate-based root canal sealers","volume":"53","author":"Torres","year":"2020","journal-title":"Int. Endod. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.joen.2024.01.001","article-title":"Characterization and assessment of physical properties of 3 single syringe hydraulic cement\u2013based sealers","volume":"50","author":"Raman","year":"2024","journal-title":"J. Endod."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1016\/j.joen.2013.06.012","article-title":"Physical properties of 5 root canal sealers","volume":"39","author":"Zhou","year":"2013","journal-title":"J. Endod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1394","DOI":"10.1111\/iej.13526","article-title":"Influence of variations in the environmental pH on the solubility and water sorption of a calcium silicate-based root canal sealer","volume":"54","author":"Silva","year":"2021","journal-title":"Int. Endod. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/j.dental.2019.04.011","article-title":"Esterases affect the physical properties of materials used to seal the endodontic space","volume":"35","author":"Marashdeh","year":"2019","journal-title":"Dent. Mater."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"7136345","DOI":"10.1155\/2018\/7136345","article-title":"Different setting conditions affect surface characteristics and microhardness of calcium silicate-based sealers","volume":"2018","author":"Yang","year":"2018","journal-title":"Scanning"},{"key":"ref_35","first-page":"265","article-title":"Setting time and surface microhardness of Mineral Trioxide Aggregate and 1% and 5% Fluoride-doped Mineral Trioxide Aggregate mixed with water and gel-like polymer","volume":"14","author":"Dianat","year":"2019","journal-title":"Iran. Endod. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"41","DOI":"10.5395\/rde.2025.50.e41","article-title":"Effect of moisture and pH on setting time and microhardness of three premixed calcium silicate-based root canal sealers: An in vitro experimental study","volume":"50","author":"Kim","year":"2025","journal-title":"Restor. Dent. Endod."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1469","DOI":"10.1016\/j.joen.2015.04.011","article-title":"Bioactivity of a calcium silicate based endodontic cement (BioRoot RCS): Interactions with human periodontal ligament cells in vitro","volume":"41","author":"Camps","year":"2015","journal-title":"J. Endod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1055\/s-0042-1750778","article-title":"In vitro cytotoxicity and mineralization potential of an endodontic bioceramic material","volume":"17","author":"Sheela","year":"2023","journal-title":"Eur. J. Dent."},{"key":"ref_39","first-page":"6617930","article-title":"Push-out bond strength, characterization, and ion release of premixed and powder-liquid bioceramic sealers with or without gutta- percha","volume":"2021","year":"2021","journal-title":"Scanning"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1111\/iej.12062","article-title":"Bioactivity evaluation of three calcium silicate-based endodontic materials","volume":"46","author":"Han","year":"2013","journal-title":"Int. Endod. J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/sca.21240","article-title":"Dynamic intratubular biomineralization following root canal obturation with pozzolan-based mineral trioxide aggregate sealer cement","volume":"38","author":"Yoo","year":"2016","journal-title":"Scanning"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1016\/j.joen.2017.11.011","article-title":"Interfacial characteristics and cytocompatibility of hydraulic sealer cements","volume":"44","author":"Benezra","year":"2018","journal-title":"J. Endod."},{"key":"ref_43","unstructured":"(2012). Dental Root Canal Sealing Materials (Standard No. ISO 6876)."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1080\/00387010.2018.1485703","article-title":"Setting reaction of new bioceramic root canal sealers","volume":"51","author":"Saleh","year":"2018","journal-title":"Spectrosc. Lett."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Abu Zeid, S.T., Alamoudi, R.A., and Mokeem Saleh, A.A. (2022). Impact of water solubility on chemical composition and surface structure of two generations of bioceramic root canal sealers. Appl. Sci., 12.","DOI":"10.3390\/app12020873"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1111\/j.1365-2591.2010.01768.x","article-title":"Apatite-forming ability (Bioactivity) of ProRoot MTA","volume":"43","author":"Gandolfi","year":"2010","journal-title":"Int. Endod. J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"458","DOI":"10.4012\/dmj.2014-309","article-title":"The Hydration Chemistry of ProRoot MTA","volume":"34","author":"Li","year":"2015","journal-title":"Dent. Mater. J."},{"key":"ref_48","first-page":"1639","article-title":"Sol-Gel synthesis of white mineral trioxide aggregate with potential use as biocement","volume":"7","author":"Voicu","year":"2012","journal-title":"Dig. J. Nanomater. Biostructures"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.molstruc.2004.02.047","article-title":"Phase stability of Hydroxyapatite-Zirconia (HAp-ZrO2) composites for bone replacement","volume":"704","author":"Paszkiewicz","year":"2004","journal-title":"J. Molec. Struct."},{"key":"ref_50","first-page":"2","article-title":"Self-setting bone cement formulations based on egg shell derived tetracalcium phosphate bioceramics","volume":"5","author":"Jayasree","year":"2015","journal-title":"Bioceram. Dev. Appl."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1016\/j.joen.2019.12.009","article-title":"Cytotoxicity and the effect of temperature on physical properties and chemical composition of a new calcium silicate\u2013based root canal sealer","volume":"46","author":"Chen","year":"2020","journal-title":"J. Endod."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"15","DOI":"10.2298\/PAC1001015R","article-title":"In vitro bioactivity of biphasic calcium phosphate silicate glass-ceramic in CaO-SiO2-P2O5 system","volume":"4","author":"Radev","year":"2010","journal-title":"Process. Appl. Ceram."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Okamura, T., Chen, L., Tsumano, N., Ikeda, C., Komasa, S., Tominaga, K., and Hashimoto, Y. (2020). Biocompatibility of a high-plasticity, calcium silicate-based, ready-to-use material. Materials, 13.","DOI":"10.3390\/ma13214770"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.cemconres.2009.01.017","article-title":"Early hydration and setting of portland cement monitored by IR, SEM and Vicat techniques","volume":"39","author":"Jaglid","year":"2009","journal-title":"Cem. Conc. Res."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.molstruc.2010.12.058","article-title":"Vibrational investigation of calcium-silicate cements for endodontics in simulated body fluids","volume":"993","author":"Taddei","year":"2011","journal-title":"J. Molec. Struct."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1016\/j.bone.2006.12.061","article-title":"Maturational changes in dentin mineral properties","volume":"40","author":"Verdelis","year":"2007","journal-title":"Bone"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1007\/s00784-020-03496-x","article-title":"Determining the setting of root canal sealers using an in vivo animal experimental model","volume":"25","author":"Silva","year":"2021","journal-title":"Clin. Oral Investig."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1590\/0103-6440202204832","article-title":"Physico-chemical properties of calcium silicate-based sealers in powder\/liquid and ready-to-use forms","volume":"33","author":"Janini","year":"2022","journal-title":"Braz. Dent. J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1111\/iej.13742","article-title":"In vitro physicochemical characterization of five root canal sealers and their influence on an ex vivo oral multi-species biofilm community","volume":"55","author":"Saavedra","year":"2022","journal-title":"Int. Endod. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1177\/0022034512443068","article-title":"Dentin-cement interfacial interaction: Calcium silicates and polyalkenoates","volume":"91","author":"Atmeh","year":"2012","journal-title":"J. Dent. Res."},{"key":"ref_61","unstructured":"(2025). Dental Root Canal Sealing Materials (Standard No. ISO 6876 (2025-11))."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0043-1648(74)90102-1","article-title":"Principles of abrasive wear","volume":"28","author":"Khruschow","year":"1974","journal-title":"Wear"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Al Akam, H., Kim, H.-C., and Jeong, J.W. (2024). Retreatment strategies for cases containing calcium silicate-based root canal sealers: A comprehensive review. Dent. J., 12.","DOI":"10.20944\/preprints202401.0194.v1"},{"key":"ref_64","first-page":"e4","article-title":"Microhardness of root canal sealers at different canal thirds: A pilot in vitro study","volume":"16","year":"2025","journal-title":"J. Oral. Maxillofac. Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1007\/s00784-025-06150-6","article-title":"Physicochemical properties and biological interaction of calcium silicate-based sealers\u2014In vivo model","volume":"29","author":"Janini","year":"2025","journal-title":"Clin. Oral. Investig."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"740","DOI":"10.1111\/aej.70013","article-title":"In vitro assessment of the effects of endodontic sealers on cell viability, adhesion, and migration","volume":"51","author":"Janini","year":"2025","journal-title":"Aust. Endod. J."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Ferreira, I., Fernandes, B., Braga, A.C., Lopes, M.A., and Pina-Vaz, I. (2025). Achieving patency in straight canals obturated with AH Plus Bioceramic sealer: An ex vivo study. Appl. Sci., 15.","DOI":"10.3390\/app15115855"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/aej.12950","article-title":"Determination of Surface Characteristics, Solubility and Ion Release of a Calcium Silicate Root Canal Sealer","volume":"51","author":"Katakidis","year":"2025","journal-title":"Aust. Endod. J."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"881","DOI":"10.4103\/JCDE.JCDE_406_25","article-title":"A comparative analysis of adhesion abilities between AH Plus\u00ae Bioceramic, Ceraseal\u00ae and AH Plus\u00ae on root canal dentine surfaces","volume":"28","author":"Maharti","year":"2025","journal-title":"J. Conserv. Dent. Endod."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Shim, E., Son, J.W., Kwon, J., Kim, H.-J., Jang, J.-H., Chang, S.W., and Oh, S. (2025). Retrieval of AH Plus Bioceramic and Ceraseal versus AH Plus in endodontic retreatment. J. Clin. Med., 14.","DOI":"10.3390\/jcm14061826"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"101400","DOI":"10.1016\/j.jobcr.2026.01.004","article-title":"Comparative analysis between resin-based root canal sealer and recent bioceramic-based root canal sealers using MicroCT, film thickness, and solubility","volume":"16","author":"Ismail","year":"2026","journal-title":"J. Oral Biol. Craniofac. Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.joen.2008.11.006","article-title":"A mineral trioxide aggregate sealer stimulated mineralization","volume":"35","author":"Watanabe","year":"2009","journal-title":"J. Endod."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.dental.2004.05.010","article-title":"The constitution of mineral trioxide aggregate","volume":"21","author":"Camilleri","year":"2005","journal-title":"Dent. Mater."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"3383","DOI":"10.1002\/jbm.b.34673","article-title":"Morphological and chemical analysis of surface interaction of irrigant-endosequence root repair material","volume":"108","author":"Alamoudi","year":"2020","journal-title":"J. Biomed. Mater. Res. B. Appl. Biomater."},{"key":"ref_75","first-page":"175","article-title":"Effect of sodium hypochlorite treatment on the molecular composition and morphology of human coronal dentin","volume":"6","author":"Mountouris","year":"2004","journal-title":"J. Adhes. Dent."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1034\/j.1600-9657.2002.00097.x","article-title":"Long-term calcium hydroxide as a root canal dressing may increase risk of root fracture","volume":"18","author":"Andreasen","year":"2002","journal-title":"Dent. Traumatol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1097\/00004770-200212000-00008","article-title":"The effect of three commonly used endodontic materials on the strength and hardness of root dentin","volume":"28","author":"White","year":"2002","journal-title":"J. Endod."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"231","DOI":"10.14744\/eej.2023.62207","article-title":"Evaluating the solubility of endodontic sealers in response to static and dynamic stress: An in vitro study","volume":"26","author":"Saghiri","year":"2024","journal-title":"Eur. Endod. J."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/j.identj.2023.04.003","article-title":"Characterization of the bioactivity and the solubility of a new root canal sealer","volume":"73","author":"Alnoury","year":"2023","journal-title":"Int. Dent. 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