{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:39:24Z","timestamp":1785436764489,"version":"3.56.0"},"reference-count":65,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technological Development and Innovation of the Republic of Serbia","award":["451-03-34\/2026-03\/200129"],"award-info":[{"award-number":["451-03-34\/2026-03\/200129"]}]},{"award":["451-03-34\/2026-03\/200129"],"award-info":[{"award-number":["451-03-34\/2026-03\/200129"]}],"id":[{"id":"https:\/\/ror.org\/01znas443","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Although biocompatible calcium silicate cements (CSCs) and calcium aluminate cements (CACs) may induce local and systemic adverse effects. This study aimed to evaluate the subchronic and chronic effects of experimental CAC and CSC mixtures on rat liver, kidney, brain, and spleen tissue. Two experimental mixtures, CAC with added ZrO2 (ECCA + ZrO2) and CSC with added ZrO2 (ECCS + ZrO2), and mineral trioxide aggregate (MTA), were implanted intraalveolary in 36 male Wistar rats. Histomorphometry was conducted after 30 and 180 days on liver, kidney, brain, and spleen. Consistent results were observed in all material groups. Liver tissue inflammation ranged from none to minimal for all three materials. In kidney, ECCA + ZrO2 displayed a slightly better result than other two materials. In brain, after 180 days, both ECCA + ZrO2 and MTA showed a statistically significant reduction in perineural vacuolation (p &lt; 0.05), and MTA showed a reduction in the percentage of intravascular congestion (p &lt; 0.05). In spleen, a larger lymphoid follicle diameter was observed for ECCS + ZrO2 chronic group compared to other two materials (p &lt; 0.05). ECCA + ZrO2, ECCS + ZrO2, and MTA caused none to minimal changes in liver, kidney, brain, and spleen following subchronic and chronic exposure.<\/jats:p>","DOI":"10.3390\/jfb17050253","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T09:12:41Z","timestamp":1779268361000},"page":"253","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Histomorphometric Evaluation of Subchronic and Chronic Effects of Novel Experimental Calcium Aluminate- and Calcium Silicate-Based Dental Cement Materials on Rat Liver, Kidney, Brain, and Spleen Tissues"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8110-1484","authenticated-orcid":false,"given":"Veljko","family":"Ili\u0107","sequence":"first","affiliation":[{"name":"School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1357-4970","authenticated-orcid":false,"given":"Sanja","family":"Milutinovi\u0107-Smiljani\u0107","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1607-4069","authenticated-orcid":false,"given":"Vladimir","family":"Bio\u010danin","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry in Pan\u010devo, University of Business Academy, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5397-9361","authenticated-orcid":false,"given":"Jovana","family":"Kuzmanovi\u0107 Pfi\u0107er","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"Tasi\u0107","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vesna","family":"Danilovi\u0107","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Japund\u017ei\u0107-\u017digon","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8617-3583","authenticated-orcid":false,"given":"Smiljana","family":"Para\u0161","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences and Mathematics, University of Banja Luka, 78000 Banja Luka, Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vukoman","family":"Jokanovi\u0107","sequence":"additional","affiliation":[{"name":"ALBOS d.o.o., 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dejan","family":"\u0106etkovi\u0107","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0110or\u0111e","family":"Antonijevi\u0107","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.joen.2009.09.006","article-title":"Mineral trioxide aggregate: A comprehensive literature review\u2014Part I: Chemical, physical, and antibacterial properties","volume":"36","author":"Parirokh","year":"2010","journal-title":"J. Endod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.14219\/jada.archive.1993.0199","article-title":"Effects of dental restorative materials: Local and systemic responses reviewed","volume":"124","author":"Stanley","year":"1993","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/eos.12238","article-title":"In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and liver aluminium levels in rats","volume":"124","author":"Demirkaya","year":"2016","journal-title":"Eur. J. Oral Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1177\/0960327116679713","article-title":"Brain aluminium accumulation and oxidative stress in the presence of calcium silicate dental cements","volume":"36","author":"Demirkaya","year":"2017","journal-title":"Hum. Exp. Toxicol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1111\/iej.14101","article-title":"Leaching and cytotoxicity of bismuth oxide in ProRoot MTA\u2014A laboratory investigation","volume":"57","author":"Pelepenko","year":"2024","journal-title":"Int. Endod. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.joen.2009.09.009","article-title":"Mineral trioxide aggregate: A comprehensive literature review\u2014Part III: Clinical applications, drawbacks, and mechanism of action","volume":"36","author":"Parirokh","year":"2010","journal-title":"J. Endod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1111\/iej.13617","article-title":"Does MTA provide a more favourable histological response than other materials in the repair of furcal perforations? A systematic review","volume":"54","author":"Pinheiro","year":"2021","journal-title":"Int. Endod. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.sdentj.2023.08.013","article-title":"Local and systemic adverse effects of nanoparticles incorporated in dental materials\u2014A critical review","volume":"36","author":"Karunakaran","year":"2024","journal-title":"Saudi Dent. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1111\/iej.12050","article-title":"Biocompatibility of BioAggregate and mineral trioxide aggregate on the liver and kidney","volume":"46","author":"Khalil","year":"2013","journal-title":"Int. Endod. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1590\/1678-7757-2016-0634","article-title":"Systemic effect of mineral aggregate-based cements: Histopathological analysis in rats","volume":"25","author":"Garcia","year":"2017","journal-title":"J. Appl. Oral Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"28913","DOI":"10.1016\/j.ceramint.2021.07.052","article-title":"Influence of the addition of different radiopacifiers and bioactive nano-hydroxyapatite on physicochemical and biological properties of calcium silicate based endodontic ceramic","volume":"47","year":"2021","journal-title":"Ceram. Int."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.dental.2020.11.014","article-title":"Physicochemical, biological, and antibacterial evaluation of tricalcium silicate based reparative cements with different radiopacifiers","volume":"37","author":"Queiroz","year":"2021","journal-title":"Dent. Mater. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"23948","DOI":"10.1016\/j.ceramint.2025.03.081","article-title":"Physicochemical and biological properties of bioactive calcium aluminate dental cement enriched with zirconium dioxide","volume":"51","year":"2025","journal-title":"Ceram. Int."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"11732","DOI":"10.1016\/j.ceramint.2016.04.092","article-title":"Calcium aluminate cement-based compositions for biomaterial applications","volume":"42","author":"Parreira","year":"2016","journal-title":"Ceram. Int."},{"key":"ref_15","first-page":"12203","article-title":"A new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis","volume":"45","year":"2019","journal-title":"Ceram. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1111\/jace.18051","article-title":"Calcium silicate and calcium aluminate cements for dentistry reviewed","volume":"105","author":"Primus","year":"2022","journal-title":"J. Am. Ceram. Soc."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"4335","DOI":"10.1016\/j.ceramint.2019.10.156","article-title":"Effect of nanosilica addition on bioactivity and in vivo properties of calcium aluminate cement","volume":"46","author":"Mohammadi","year":"2020","journal-title":"Ceram. Int."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"505","DOI":"10.2298\/SOS231203065C","article-title":"The influence of different radiopacifying agents on hermetical sealing ability of calcium silicate and calcium aluminate dental cements","volume":"56","author":"Cetkovic","year":"2024","journal-title":"Sci. Sinter."},{"key":"ref_19","first-page":"323","article-title":"Surface morphology, compressive strength and biocompatibility of calcium aluminate dental cement","volume":"Volume 2","year":"2022","journal-title":"Proceedings of the International Conference on Fundamental and Applied Aspects of Physical Chemistry"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1111\/iej.13105","article-title":"Influence of nanostructured calcium aluminate and calcium silicate on the liver: Histological and unbiased stereological analysis","volume":"52","year":"2019","journal-title":"Int. Endod. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1111\/iej.13567","article-title":"Comparison of Bio-C Pulpo and MTA Repair HP with White MTA: Effect on liver parameters and evaluation of biocompatibility and bioactivity in rats","volume":"54","author":"Delfino","year":"2021","journal-title":"Int. Endod. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1111\/j.1365-2591.2010.01711.x","article-title":"A comparative study of dental pulp response to several pulpotomy agents","volume":"43","author":"Tabarsi","year":"2010","journal-title":"Int. Endod. J."},{"key":"ref_23","first-page":"34","article-title":"Histopathological pulp response of teeth capped with calcium aluminate cement and biodentine: Experimental study on rodents","volume":"73","author":"Arbutina","year":"2024","journal-title":"Veterinaria"},{"key":"ref_24","unstructured":"(2022). Biological Evaluation of Medical Devices. Part 2: Animal Welfare Requirements (Standard No. ISO 10993-2)."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1590\/S1678-77572010000100013","article-title":"Evaluation of subcutaneous and alveolar implantation surgical sites in the study of the biological properties of root-end filling endodontic materials","volume":"18","author":"Cintra","year":"2010","journal-title":"J. Appl. Oral Sci."},{"key":"ref_26","unstructured":"(2018). Dentistry\u2014Evaluation of Biocompatibility of Medical Devices Used in Dentistry (Standard No. ISO 7405)."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"102516","DOI":"10.1016\/j.mex.2023.102516","article-title":"Rodent brain extraction and dissection: A comprehensive approach","volume":"12","author":"Aboghazleh","year":"2024","journal-title":"MethodsX"},{"key":"ref_28","unstructured":"Smith, D.G., and Schenk, M.P. (2001). A Dissection Guide & Atlas to the Rat, Morton Publishing Company."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/joa.12585","article-title":"Stereological analysis of size and density of hepatocytes in the porcine liver","volume":"230","author":"Junatas","year":"2017","journal-title":"J. Anat."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1002\/hep.1840010511","article-title":"Formulation and application of a numerical scoring system for assessing histological activity in asymptomatic chronic active hepatitis","volume":"1","author":"Knodell","year":"1981","journal-title":"Hepatology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s12199-018-0707-2","article-title":"Effect of fluoride on major organs with the different time of exposure in rats","volume":"23","author":"Perera","year":"2018","journal-title":"Environ. Health Prev. Med."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Arjmand, A., Tsipouras, M.G., Tzallas, A.T., Forlano, R., Manousou, P., and Giannakeas, N. (2020). Quantification of liver fibrosis-A comparative study. Appl. Sci., 10.","DOI":"10.3390\/app10020447"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"153","DOI":"10.4081\/aiua.2020.2.153","article-title":"Protective effect of chlorogenic acid on renal ischemia\/reperfusion injury in rats","volume":"92","author":"Toprak","year":"2020","journal-title":"Arch. Ital. Urol. Androl."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.jmau.2015.10.001","article-title":"Histological study on the protective role of ascorbic acid on cadmium-induced cerebral cortical neurotoxicity in adult male albino rats","volume":"4","author":"Afifi","year":"2016","journal-title":"J. Microsc. Ultrastruct."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6493286","DOI":"10.1155\/2019\/6493286","article-title":"Histological, biochemical, and hematological effects of Goniothalamin on selective internal organs of male Sprague-Dawley rats","volume":"2019","author":"Kaid","year":"2019","journal-title":"J. Toxicol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Abd-Elhakim, Y.M., El Sharkawy, N.I., Gharib, H.S.A., Hassan, M.A., Metwally, M.M.M., Elbohi, K.M., Hassan, B.A., and Mohammed, A.T. (2023). Neurobehavioral responses and toxic brain reactions of juvenile rats exposed to Iprodione and Chlorpyrifos, alone and in a mixture. Toxics, 11.","DOI":"10.3390\/toxics11050431"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"121039","DOI":"10.1016\/j.biomaterials.2021.121039","article-title":"Potential neurotoxicity of titanium implants: Prospective, in-vivo and in-vitro study","volume":"276","author":"Shelly","year":"2021","journal-title":"Biomaterials"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1111\/iep.12080","article-title":"A potential link among antioxidant enzymes, histopathology and trace elements in canine visceral leishmaniasis","volume":"95","author":"Souza","year":"2014","journal-title":"Int. J. Exp. Pathol."},{"key":"ref_39","first-page":"187","article-title":"Anatomical and histological alterations of the spleen in rat, Rattus norvegicus exposed to mercury","volume":"9","author":"Kareem","year":"2022","journal-title":"Iran. J. Ichthyol."},{"key":"ref_40","unstructured":"(2018). Biological Evaluation of Medical Devices. Part 1: Evaluation and Testing Within a Risk Management Process (Standard No. ISO 10993-1)."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1046\/j.1440-1681.1999.03048.x","article-title":"Kinetic aspects of drug disposition in the lungs","volume":"26","author":"Upton","year":"1999","journal-title":"Clin. Exp. Pharmacol. Physiol."},{"key":"ref_42","first-page":"60","article-title":"Magnetic cell-homing strategy for autologous dental pulp regeneration as an alternative in necrotic teeth: A proof-of-concept study","volume":"52","author":"Babo","year":"2025","journal-title":"Eur. Cell Mater."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.joen.2010.10.011","article-title":"An animal model to study regenerative endodontics","volume":"37","author":"Torabinejad","year":"2011","journal-title":"J. Endod."},{"key":"ref_44","first-page":"207","article-title":"The mighty mouse: The impact of rodents on advances in biomedical research","volume":"110","author":"Bryda","year":"2013","journal-title":"Mo. Med."},{"key":"ref_45","unstructured":"(2017). Biological Evaluation of Medical Devices. Part 11: Tests for Systemic Toxicity (Standard No. ISO 10993-11)."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1111\/iej.13519","article-title":"Inflammatory profile of apical periodontitis associated with liver fibrosis in rats: Histological and immunohistochemical analysis","volume":"54","author":"Estrela","year":"2021","journal-title":"Int. Endod. J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s42826-024-00198-3","article-title":"3R-refinement principles: Elevating rodent well-being and research quality","volume":"40","author":"Rinwa","year":"2024","journal-title":"Lab. Anim. Res."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e15376","DOI":"10.7717\/peerj.15376","article-title":"Systemic effect of calcium silicate-based cements with different radiopacifiers\u2014Histopathological analysis in rats","volume":"11","author":"Atas","year":"2023","journal-title":"PeerJ"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Para\u0161, S., Tri\u0161i\u0107, D., Mitrovi\u0107 Ajti\u0107, O., Antonijevi\u0107, \u0110., \u010colovi\u0107, B., Drobne, D., and Jokanovi\u0107, V. (2021). Biocompatibility study of a new dental cement based on hydroxyapatite and calcium silicates: Focus on liver, kidney, and spleen tissue effects. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22115468"},{"key":"ref_50","first-page":"624","article-title":"The laboratory rat: Relating its age with human\u2019s","volume":"4","author":"Sengupta","year":"2013","journal-title":"Int. J. Prev. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/0030-4220(66)90077-6","article-title":"Reaction of rat connective tissue to polyethylene tube implants","volume":"21","author":"Torneck","year":"1966","journal-title":"Oral Surg. Oral Med. Oral Pathol."},{"key":"ref_52","unstructured":"(2016). Biological Evaluation of Medical Devices (Standard No. ISO 10993-6). Part 6: Tests for Local Effects After Implantation."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/JPER.22-0091","article-title":"Ion release and local effects of titanium metal particles from dental implants: An experimental study in rats","volume":"94","year":"2023","journal-title":"J. Periodontol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.tripleo.2008.07.018","article-title":"Presence of arsenic in different types of MTA and white and gray Portland cement","volume":"106","author":"Bramante","year":"2008","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1016\/j.joen.2011.08.020","article-title":"Heavy metal analysis of ortho MTA and ProRoot MTA","volume":"37","author":"Chang","year":"2021","journal-title":"J. Endod."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3413","DOI":"10.3892\/mmr.2017.6389","article-title":"Kidney and heavy metals\u2014The role of environmental exposure (Review)","volume":"15","author":"Lentini","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1111\/iej.12435","article-title":"In vivo evaluation of the inflammatory response and IL-6 immunoexpression promoted by Biodentine and MTA Angelus","volume":"49","author":"Cerri","year":"2016","journal-title":"Int. Endod. J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1111\/iej.13398","article-title":"Evaluation of the biological properties of two experimental calcium silicate sealers: An in vivo study in rats","volume":"54","author":"Silva","year":"2021","journal-title":"Int. Endod. J."},{"key":"ref_59","first-page":"390","article-title":"Pyramidal layer thinning, shrunken neurons and deep vacuolation in hippocampus due to the organic lead induced toxicity","volume":"2","author":"Zulfiqar","year":"2014","journal-title":"Int. J. Anat. Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/dneu.22919","article-title":"Development of the hippocampal CA2 region and the emergence of social recognition","volume":"83","author":"Diethorn","year":"2023","journal-title":"Dev. Neurobiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1177\/0192623310391480","article-title":"Preparation and Analysis of the Central Nervous System","volume":"39","author":"Jordan","year":"2010","journal-title":"Toxicol. Pathol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0896-6273(03)00601-9","article-title":"Diversity in the mechanisms of neuronal cell death","volume":"40","author":"Yuan","year":"2003","journal-title":"Neuron"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"eaau6085","DOI":"10.1126\/sciimmunol.aau6085","article-title":"Structure and function of the immune system in the spleen","volume":"4","author":"Lewis","year":"2019","journal-title":"Sci. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.3324\/haematol.2009.005983","article-title":"Chronic inflammatory disease, lymphoid tissue neogenesis and extranodal marginal zone B-cell lymphomas","volume":"94","author":"Bende","year":"2009","journal-title":"Haematologica"},{"key":"ref_65","first-page":"243","article-title":"Ovariectomy and chronic stress: Effects on the morphometry of the spleen of rats","volume":"38","author":"Santana","year":"2021","journal-title":"J. Morphol. Sci."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/5\/253\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T10:00:44Z","timestamp":1779271244000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/5\/253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":65,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["jfb17050253"],"URL":"https:\/\/doi.org\/10.3390\/jfb17050253","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]}}}