{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T09:06:23Z","timestamp":1779699983150,"version":"3.53.1"},"reference-count":28,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>The increasing adoption of digital workflows in implant dentistry relies heavily on the accuracy of implant scan bodies (ISBs), which may be affected by repeated sterilization. This in vitro study evaluated the effect of 50 steam sterilization cycles on the dimensional stability of polymer and titanium ISBs. A total of 100 test specimens (n = 50 per material) were scanned before (T0) and after sterilization (T50) using a high-resolution intraoral scanner, generating 900 STL datasets for metrological analysis. Surface deviation, linear displacement, and angular deviation were assessed using validated industrial and dental software, with statistical evaluation performed through paired tests and linear mixed-effects models. Both materials exhibited statistically significant dimensional changes after sterilization (p &lt; 0.001). Titanium scan bodies demonstrated greater linear deformation (69.76 \u03bcm) compared to polymer ones (49.50 \u03bcm), while maintaining superior angular stability (0.21\u00b0 vs. \u22121.69\u00b0 mean angular change in the polymer group). A significant interaction between material type and sterilization was observed. Despite high baseline precision, repeated autoclaving induced clinically relevant deviations in both materials. These findings indicate that cumulative sterilization cycles adversely affect ISB accuracy and highlight the importance of adhering to manufacturer recommendations to ensure optimal prosthetic outcomes.<\/jats:p>","DOI":"10.3390\/jfb17050255","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T14:12:54Z","timestamp":1779286374000},"page":"255","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dimensional Stability of Polymer and Titanium Implant Scan Bodies After Repeated Steam Sterilization: A High-Resolution 3D Metrological In Vitro Study"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1024-5509","authenticated-orcid":false,"given":"Igor","family":"Smojver","sequence":"first","affiliation":[{"name":"Department of Oral Surgery, School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7092-8045","authenticated-orcid":false,"given":"Roko","family":"Bjelica","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1719-3125","authenticated-orcid":false,"given":"Yuval","family":"Reiser","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0020-5247","authenticated-orcid":false,"given":"Marko","family":"Vuleti\u0107","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"},{"name":"Department of Dental Medicine, Clinical Hospital Centre Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6058-1503","authenticated-orcid":false,"given":"Vladimir","family":"Prpi\u0107","sequence":"additional","affiliation":[{"name":"Department of Fixed Prosthodontics, School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0213-1170","authenticated-orcid":false,"given":"Dragana","family":"Gabri\u0107","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia"},{"name":"Department of Dental Medicine, Clinical Hospital Centre Zagreb, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105152","DOI":"10.1016\/j.jdent.2024.105152","article-title":"Intraoral scanners in implant prosthodontics: A narrative review","volume":"148","author":"Pletkus","year":"2024","journal-title":"J. Dent."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e70038","DOI":"10.1111\/cid.70038","article-title":"Digital Workflow and Guided Surgery in Implant Therapy: Literature Review and Practical Tips to Optimize Precision","volume":"27","author":"Chen","year":"2025","journal-title":"Clin. Implant Dent. Relat. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1016\/j.prosdent.2025.10.007","article-title":"Implant scanning workflows: The implant pentagon","volume":"134","year":"2025","journal-title":"J. Prosthet. Dent."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1111\/jerd.13004","article-title":"Digital workflow in implant prosthodontics: The critical aspects for reliable accuracy","volume":"35","author":"Gracis","year":"2023","journal-title":"J. Esthet. Restor. Dent."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Marques, S., Ribeiro, P., Falc\u00e3o, C., Lemos, B.F., R\u00edos-Carrasco, B., R\u00edos-Santos, J.V., and Herrero-Climent, M. (2021). Digital impressions in implant dentistry: A literature review. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18031020"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Meneghetti, P.C., Li, J., Borella, P.S., Mendon\u00e7a, G., and Burnett, L.H. (2023). Influence of scanbody design and intraoral scanner on the trueness of complete arch implant digital impressions: An in vitro study. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0295790"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/jopr.13749","article-title":"Intraoral digital implant scans: Parameters to improve accuracy","volume":"32","author":"Lanis","year":"2023","journal-title":"J. Prosthodont."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Costa Santos, F.H.P., de Melo, P.S., Borella, P.S., Neves, F.D.D., and Zancope, K. (2025). Effect of autoclaving process on trueness and qualitative marginal fit of intraoral scan bodies manufactured from two materials: An in vitro study. PLoS ONE, 20.","DOI":"10.1371\/journal.pone.0325068"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Kato, T., Yasunami, N., Furuhashi, A., Sanda, K., and Ayukawa, Y. (2022). Effects of autoclave sterilization and multiple use on implant scanbody deformation in vitro. Materials, 15.","DOI":"10.3390\/ma15217717"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1324.e1","DOI":"10.1016\/j.prosdent.2025.02.007","article-title":"Effect of reusing impression posts and scan bodies on recording accuracy","volume":"133","author":"Aktas","year":"2025","journal-title":"J. Prosthet. Dent."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"105045","DOI":"10.1016\/j.jdent.2024.105045","article-title":"Effect of different impression coping and scan body designs on the accuracy of conventional versus digital implant impressions: An in vitro study","volume":"146","author":"Alkindi","year":"2024","journal-title":"J. Dent."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1111\/jerd.12985","article-title":"A guide for maximizing the accuracy of intraoral digital scans. Part 1: Operator factors","volume":"35","author":"Kois","year":"2023","journal-title":"J. Esthet. Restor. Dent."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Qin, L., Yao, S., Zhao, J., Zhou, C., Oates, T.W., Weir, M.D., Wu, J., and Xu, H.H.K. (2021). Review on development and dental applications of polyetheretherketone-based biomaterials and restorations. Materials, 14.","DOI":"10.3390\/ma14020408"},{"key":"ref_14","first-page":"448","article-title":"High-performance polymers and their potential application as medical and oral implant materials: A review","volume":"24","author":"Wiesli","year":"2015","journal-title":"Implant Dent."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.prosdent.2022.09.010","article-title":"Magnitude of misfit threshold in implant-supported restorations: A systematic review","volume":"132","author":"Abdelrehim","year":"2024","journal-title":"J. Prosthet. Dent."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1111\/cid.13187","article-title":"Effect of material type, torque value, and sterilization on linear displacements of a scan body: An in vitro study","volume":"25","author":"Diker","year":"2023","journal-title":"Clin. Implant Dent. Relat. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/j.prosdent.2022.04.009","article-title":"Misfit simulation on implant prostheses with different combinations of engaging and nonengaging titanium bases. Part 1: Stereomicroscopic assessment of the active and passive fit","volume":"129","author":"Rutkunas","year":"2023","journal-title":"J. Prosthet. Dent."},{"key":"ref_18","first-page":"947","article-title":"Bone loading caused by different types of misfits of implant-supported fixed dental prostheses: A three-dimensional finite element analysis based on experimental results","volume":"25","author":"Winter","year":"2010","journal-title":"Int. J. Oral Maxillofac. Implant."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"891","DOI":"10.11607\/jomi.6945","article-title":"Effect of multiple use of impression copings and scanbodies on implant cast accuracy","volume":"34","author":"Sawyers","year":"2019","journal-title":"Int. J. Oral Maxillofac. Implant."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Revilla-Le\u00f3n, M., Oh, K.C., Barmak, A.B., Park, J.M., and Kois, J.C. (2026). Influence of protocol and number of sterilization cycles on the accuracy of an additively manufactured extraoral scan body fabricated with two biocompatible materials. J. Prosthet. Dent., in press.","DOI":"10.1016\/j.prosdent.2026.01.017"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1111\/jre.12834","article-title":"Remnant oral biofilm and microorganisms after autoclaving sterilization of retrieved healing abutments","volume":"56","author":"Barreiros","year":"2021","journal-title":"J. Periodontal Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Sharon, E., Pietrokovski, Y., Engel, I., Assali, R., Houri-Haddad, Y., and Beyth, N. (2023). Biocompatibility, surface morphology, and bacterial load of dental implant abutments following decontamination protocols: An in vitro study. Materials, 16.","DOI":"10.3390\/ma16114080"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1111\/clr.14136","article-title":"Decontamination of biofilm-contaminated implant surfaces: An in vitro evaluation","volume":"34","author":"Ichioka","year":"2023","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_24","first-page":"1","article-title":"Surface modifications of dental implants for enhanced antimicrobial properties: A literature review","volume":"7","author":"Reiser","year":"2025","journal-title":"Environ. Dent. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/clr.14054","article-title":"Biofilm formation on dental implants with a hybrid surface microtopography: An in vitro study in a validated multispecies dynamic biofilm model","volume":"34","author":"Bravo","year":"2023","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/j.1600-0501.2009.01815.x","article-title":"In vivo and in vitro biofilm formation on two different titanium implant surfaces","volume":"21","author":"Gerlach","year":"2010","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.archoralbio.2013.09.013","article-title":"Microbiological and immuno-pathological aspects of peri-implant diseases","volume":"59","author":"Belibasakis","year":"2014","journal-title":"Arch. Oral Biol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Wilson, T.G. (2025). Peri-implantitis pathogenesis: Novel insights may guide therapeutic interventions. J. Periodontol., Early View.","DOI":"10.1002\/jper.70037"}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/5\/255\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T08:39:22Z","timestamp":1779698362000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/5\/255"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":28,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["jfb17050255"],"URL":"https:\/\/doi.org\/10.3390\/jfb17050255","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]}}}