{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:40:45Z","timestamp":1785426045038,"version":"3.56.0"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["TO 198\/27-1"],"award-info":[{"award-number":["TO 198\/27-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["PO 1439\/7-1"],"award-info":[{"award-number":["PO 1439\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["467572288"],"award-info":[{"award-number":["467572288"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"award":["TO 198\/27-1"],"award-info":[{"award-number":["TO 198\/27-1"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"award":["PO 1439\/7-1"],"award-info":[{"award-number":["PO 1439\/7-1"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]},{"award":["467572288"],"award-info":[{"award-number":["467572288"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Dental composite dust generated during finishing procedures or mastication may adversely affect gingival epithelia. However, the mechanistic distinction between particulate and chemical (eluate) exposures and their respective signaling consequences remains insufficiently defined. Dust particles and corresponding eluates from three restorative composites, Admira Fusion, Ceram.x Spectra ST, and Filtek Supreme XTE, were evaluated under standardized high-dose in vitro exposure conditions. Human gingival keratinocytes were assessed for proliferation, adhesion, differentiation, fibronectin (FN1) remodeling, and IL-8 secretion, alongside analysis of ERK, p38, and NF-\u03baB signaling and phosphorylation of the stress-responsive regulator SIRT1 at Ser682 (SIRT1-S682). Particulate exposure elicited more pronounced impairment of cellular adhesion, proliferation, and differentiation than eluates. Dusts derived from Ceram.x Spectra ST and Filtek Supreme XTE suppressed ERK activity, reduced FN1 abundance, and decreased nuclear SIRT1-S682, consistent with a generalized stress response. In contrast, Admira Fusion dust preserved FN1, activated ERK signaling, reduced SIRT1-S682, and induced robust IL-8 secretion. Across all materials, particulate exposure reduced nuclear SIRT1-S682 without affecting total SIRT1 levels, indicating a shared permissive stress modification. Notably, only Admira Fusion coupled this permissive state with p38 activation and sustained NF-\u03baB p65 Ser536 phosphorylation, resulting in transcriptionally active NF-\u03baB and elevated IL-8 production, whereas Ceram.x Spectra ST and Filtek Supreme XTE failed to activate this ERK\u2013FN1\u2013p38\u2013NF-\u03baB axis, yielding either transcriptionally inactive NF-\u03baB or no detectable enrichment. These findings support a material-associated in vitro response pattern in which a shared SIRT1-S682 reduction is accompanied by distinct ERK\/FN1, p38, NF-\u03baB, and IL-8 readouts. SIRT1-S682 reduction alone did not define the inflammatory phenotype, because it occurred across particulate exposures, whereas IL-8 secretion was observed only under conditions that also showed p38 activation and comparatively maintained NF-\u03baB p65 Ser536 phosphorylation. This signature arises from the convergence of a permissive SIRT1-S682 background with ERK- and p38-dependent MAPK signaling to enable NF-\u03baB-mediated IL-8 expression, highlighting that both composite composition and particulate properties critically determine inflammatory potential and underscoring the importance of incorporating particulate fractions into cytocompatibility testing strategies.<\/jats:p>","DOI":"10.3390\/jfb17060264","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T15:07:52Z","timestamp":1780326472000},"page":"264","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Dusty Affair: SIRT1-S682 Modulation Orchestrates ERK\u2013FN1\u2013p38\u2013NF-\u03baB Signaling and Composite-Dependent IL-8 Responses in Gingival Keratinocytes Exposed to Dental Dust and Eluates"],"prefix":"10.3390","volume":"17","author":[{"given":"Shuoqiu","family":"Bai","sequence":"first","affiliation":[{"name":"Division of Oral Biotechnology, Center for Dental Medicine, Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0140-5221","authenticated-orcid":false,"given":"Sibylle Johanna","family":"Rau","sequence":"additional","affiliation":[{"name":"Division of Oral Biotechnology, Center for Dental Medicine, Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany"},{"name":"Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Center\u2014University of Freiburg, Medical Faculty, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1924-2694","authenticated-orcid":false,"given":"Thorsten","family":"Steinberg","sequence":"additional","affiliation":[{"name":"Division of Oral Biotechnology, Center for Dental Medicine, Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Tomakidi","sequence":"additional","affiliation":[{"name":"Division of Oral Biotechnology, Center for Dental Medicine, Medical Center\u2014University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Polydorou","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Periodontology, Faculty of Medicine, Ulm University, Ulm University Hospital, Albert-Einstein-Allee 11, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Han, B., and Wang, L. (2024). Global trend and hotspot of resin materials for dental caries repair: A bibliometric analysis. Front. Mater., 11.","DOI":"10.3389\/fmats.2024.1337972"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Kucharska, K., Lehmann, A., Ortarzewska, M., Jankowski, J., and Nijakowski, K. (2025). Composite Dust Toxicity Related to Restoration Polishing: A Systematic Review. J. Compos. Sci., 9.","DOI":"10.3390\/jcs9020090"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s44445-026-00139-x","article-title":"Micro and nanoplastics in dentistry: Emerging sources, health implications, and mitigation pathways: A narrative review","volume":"38","author":"Chaudhary","year":"2026","journal-title":"Saudi Dent. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.dental.2023.06.006","article-title":"Method development for the intraoral release of nanoparticles from dental restorative materials","volume":"39","author":"Kleinvogel","year":"2023","journal-title":"Dent. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5888","DOI":"10.7150\/ijbs.104351","article-title":"Development of Advanced Oral-on-a-Chip: Replicating the Intricate Human Oral Microenvironment","volume":"20","author":"Kim","year":"2024","journal-title":"Int. J. Biol. Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1016\/j.biomaterials.2013.03.019","article-title":"A review of adaptive mechanisms in cell responses towards oxidative stress caused by dental resin monomers","volume":"34","author":"Krifka","year":"2013","journal-title":"Biomaterials"},{"key":"ref_7","first-page":"2223223","article-title":"Biological aspects of modern dental composites","volume":"10","author":"Samuelsen","year":"2023","journal-title":"Biomater. Investig. Dent."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1007\/s00784-023-04910-w","article-title":"Characterization of dental dust particles and their pathogenicity to respiratory system: A narrative review","volume":"27","author":"Ding","year":"2023","journal-title":"Clin. Oral Investig."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/s41415-022-4241-7","article-title":"Resin-based composite materials: Elution and pollution","volume":"232","author":"Mulligan","year":"2022","journal-title":"Br. Dent. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"69","DOI":"10.5334\/aogh.4861","article-title":"Microplastics in the Pathogenesis of Periodontal Diseases: A Narrative Review","volume":"91","author":"Francis","year":"2025","journal-title":"Ann. Glob. Health"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"\u0160imunovi\u0107, L., Ba\u010di\u0107, I., and Me\u0161trovi\u0107, S. (2025). Micro- and Nanoplastics in Dentistry: Challenges in Obtaining High-Quality Evidence. Materials, 18.","DOI":"10.3390\/ma18184269"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Yang, Y., Liu, Y., Wang, Y., Chao, Y., Zhang, J., Jia, Y., Tie, J., and Hu, D. (2022). Regulation of SIRT1 and its roles in inflammation. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.831168"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kelsh, R., You, R., Horzempa, C., Zheng, M., and McKeown-Longo, P.J. (2014). Regulation of the innate immune response by fibronectin: Synergism between the III-1 and EDA domains. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0102974"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Holthaus, K.B., and Eckhart, L. (2024). Development-associated genes of the epidermal differentiation complex (EDC). J. Dev. Biol., 12.","DOI":"10.3390\/jdb12010004"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1038\/labinvest.3700178","article-title":"Extracellular signal-regulated kinase (ERK) in glucose-induced and endothelin-mediated fibronectin synthesis","volume":"84","author":"Xin","year":"2004","journal-title":"Lab. Investig."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/cdd.2015.75","article-title":"HIPK2 restricts SIRT1 activity upon severe DNA damage by a phosphorylation-controlled mechanism","volume":"23","author":"Conrad","year":"2016","journal-title":"Cell Death Differ."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s44466-025-00014-0","article-title":"NF-\u03baB: Master regulator of cellular responses in health and disease","volume":"1","author":"Hoffmann","year":"2025","journal-title":"Immun. Inflamm."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1016\/j.dental.2023.09.004","article-title":"Effect of dental composite dust on human gingival keratinocytes","volume":"39","author":"Himmelsbach","year":"2023","journal-title":"Dent. Mater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1016\/j.dental.2022.09.018","article-title":"Cytotoxic and inflammatory response of human lung epithelial cells A549 to particles released from dental restorative materials during dry and wet grinding","volume":"38","author":"Jiang","year":"2022","journal-title":"Dent. Mater."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Hammond, J., Maher, B.A., Gonet, T., Bautista, F., and Allsop, D. (2022). Oxidative Stress, Cytotoxic and Inflammatory Effects of Urban Ultrafine Road-Deposited Dust from the UK and Mexico in Human Epithelial Lung (Calu-3) Cells. Antioxidants, 11.","DOI":"10.3390\/antiox11091814"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1111\/j.1432-0436.2006.00099.x","article-title":"Organotypic co-cultures allow for immortalized human gingival keratinocytes to reconstitute a gingival epithelial phenotype in vitro","volume":"74","author":"Steinberg","year":"2006","journal-title":"Differentiation"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1111\/j.1600-0765.2007.01019.x","article-title":"Establishment and characterization of immortalized human gingival keratinocyte cell lines","volume":"43","author":"Michel","year":"2008","journal-title":"J. Periodontal Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Artaza-Irigaray, C., Molina-Pineda, A., Aguilar-Lemarroy, A., Ortiz-Lazareno, P., Lim\u00f3n-Toledo, L.P., Pereira-Su\u00e1rez, A.L., Rojo-Contreras, W., and Jave-Su\u00e1rez, L.F. (2019). E6\/E7 and E6(*) From HPV16 and HPV18 Upregulate IL-6 Expression Independently of p53 in Keratinocytes. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.01676"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"010502","DOI":"10.11613\/BM.2021.010502","article-title":"Sample size, power and effect size revisited: Simplified and practical approaches in pre-clinical, clinical and laboratory studies","volume":"31","author":"Serdar","year":"2021","journal-title":"Biochem. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3861","DOI":"10.3390\/ijms10093861","article-title":"Molecular toxicology of substances released from resin\u2013based dental restorative materials","volume":"10","author":"Bakopoulou","year":"2009","journal-title":"Int. J. Mol. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.dental.2009.08.014","article-title":"Eluted substances from unpolymerized and polymerized dental restorative materials and their Nernst partition coefficient","volume":"26","author":"Durner","year":"2010","journal-title":"Dent. Mater."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"618","DOI":"10.5604\/12321966.1235159","article-title":"Uncompleted polymerization and cytotoxicity of dental restorative materials as potential health risk factors","volume":"24","author":"Tykarska","year":"2017","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sabourian, P., Yazdani, G., Ashraf, S.S., Frounchi, M., Mashayekhan, S., Kiani, S., and Kakkar, A. (2020). Effect of physico-chemical properties of nanoparticles on their intracellular uptake. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21218019"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.actbio.2013.09.044","article-title":"Nanoparticle release from dental composites","volume":"10","author":"Hellack","year":"2014","journal-title":"Acta Biomater."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s00784-018-2419-9","article-title":"Cytotoxic effects to mouse and human gingival fibroblasts of a nanohybrid ormocer versus dimethacrylate-based composites","volume":"23","author":"Schubert","year":"2019","journal-title":"Clin. Oral Investig."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1093\/toxsci\/kfu273","article-title":"Macrophage solubilization and cytotoxicity of indium-containing particles as in vitro correlates to pulmonary toxicity in vivo","volume":"144","author":"Gwinn","year":"2015","journal-title":"Toxicol. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Pituru, S.M., Greabu, M., Totan, A., Imre, M., Pantea, M., Spinu, T., Tancu, A.M.C., Popoviciu, N.O., Stanescu, I.-I., and Ionescu, E. (2020). A review on the biocompatibility of PMMA-based dental materials for interim prosthetic restorations with a glimpse into their modern manufacturing techniques. Materials, 13.","DOI":"10.3390\/ma13132894"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1016\/S0142-9612(00)00037-5","article-title":"Organotypic cultures of gingival cells: An epithelial model to assess putative local effects of orthodontic plate and occlusal splint materials under more tissue-like conditions","volume":"21","author":"Tomakidi","year":"2000","journal-title":"Biomaterials"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1038\/sj.ki.5000329","article-title":"Ras modulation of superoxide activates ERK-dependent fibronectin expression in diabetes-induced renal injuries","volume":"69","author":"Lin","year":"2006","journal-title":"Kidney Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"L307","DOI":"10.1152\/ajplung.00086.2002","article-title":"Signaling intermediates required for NF-\u03baB activation and IL-8 expression in CF bronchial epithelial cells","volume":"284","author":"Li","year":"2003","journal-title":"Am. J. Physiol.-Lung Cell. Mol. Physiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1042\/BST20220271","article-title":"ERK1\/2 in immune signalling","volume":"50","author":"Lucas","year":"2022","journal-title":"Biochem. Soc. Trans."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Maddali, P., Ambesi, A., and McKeown-Longo, P.J. (2023). Induction of pro-inflammatory genes by fibronectin DAMPs in three fibroblast cell lines: Role of TAK1 and MAP kinases. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0286390"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1165\/ajrcmb.27.1.4766","article-title":"Role of MAP kinase activation in interleukin-8 production by human BEAS-2B bronchial epithelial cells submitted to cyclic stretch","volume":"27","author":"Oudin","year":"2002","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1111\/j.1523-1755.2005.00667.x","article-title":"Soluble fibronectin induces chemokine gene expression in renal tubular epithelial cells","volume":"68","author":"Ren","year":"2005","journal-title":"Kidney Int."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1165\/rcmb.2002-0261OC","article-title":"Ras and Mitogen-Activated Protein Kinase Kinase Kinase-1 Coregulate Activator Protein-1\u2013and Nuclear Factor-\u03baB\u2013Mediated Gene Expression in Airway Epithelial Cells","volume":"28","author":"Zhou","year":"2003","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1016\/j.cell.2008.10.025","article-title":"DNA damage-induced alterations in chromatin contribute to genomic integrity and age-related changes in gene expression","volume":"135","author":"Oberdoerffer","year":"2008","journal-title":"Cell"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Yang, H., Zhang, W., Pan, H., Feldser, H.G., Lainez, E., Miller, C., Leung, S., Zhong, Z., Zhao, H., and Sweitzer, S. (2012). SIRT1 Activators Suppress Inflammatory Responses through Promotion of p65 Deacetylation and Inhibition of NF-\u03baB Activity. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0046364"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"111394","DOI":"10.1016\/j.exger.2021.111394","article-title":"SIRT1 suppresses cellular senescence and inflammatory cytokine release in human dermal fibroblasts by promoting the deacetylation of NF-\u03baB and activating autophagy","volume":"150","author":"Sung","year":"2021","journal-title":"Exp. Gerontol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Takeuchi, H., Hirano, T., Whitmore, S.E., Morisaki, I., Amano, A., and Lamont, R.J. (2013). The serine phosphatase SerB of Porphyromonas gingivalis suppresses IL-8 production by dephosphorylation of NF-\u03baB RelA\/p65. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003326"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/j.taap.2005.11.001","article-title":"p38 Mitogen-activated protein kinase mediates IL-8 induction by the ribotoxin deoxynivalenol in human monocytes","volume":"213","author":"Islam","year":"2006","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1016\/j.dental.2024.05.011","article-title":"Cytotoxicity effects and differentiation potential of ormocer-based and nanohybrid composite resins on human dental pulp stem cells","volume":"40","author":"Rengo","year":"2024","journal-title":"Dent. Mater."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e212","DOI":"10.1016\/j.dental.2017.01.019","article-title":"Survival of directly placed ormocer-based restorative materials: A systematic review and meta-analysis of clinical trials","volume":"33","author":"Monsarrat","year":"2017","journal-title":"Dent. Mater."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/6\/264\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T04:34:30Z","timestamp":1780461270000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/6\/264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,1]]},"references-count":47,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["jfb17060264"],"URL":"https:\/\/doi.org\/10.3390\/jfb17060264","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,1]]}}}