{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:29:30Z","timestamp":1785403770732,"version":"3.56.0"},"reference-count":42,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["18.18.160.557"],"award-info":[{"award-number":["18.18.160.557"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education","doi-asserted-by":"publisher","award":["RID\/SP\/0048\/2024\/01"],"award-info":[{"award-number":["RID\/SP\/0048\/2024\/01"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"award":["18.18.160.557"],"award-info":[{"award-number":["18.18.160.557"]}],"id":[{"id":"https:\/\/ror.org\/05dwvd537","id-type":"ROR","asserted-by":"publisher"}]},{"award":["RID\/SP\/0048\/2024\/01"],"award-info":[{"award-number":["RID\/SP\/0048\/2024\/01"]}],"id":[{"id":"https:\/\/ror.org\/05dwvd537","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>Numerous surface modification strategies, particularly nanoengineering approaches, have been explored to tailor the physicochemical and topographical properties of titanium surfaces in order to enhance osteogenic responses at the implant interface. In this study, we propose an alkaline ozonation strategy as a novel approach to generate nanostructured TiO2 layers on Ti-6Al-4V alloy surfaces. Titanium discs were treated in a 6 M KOH solution under continuous bubbling of ozone, allowing the formation of reactive oxygen species (ROS) responsible for oxidative surface restructuring. Scanning electron microscopy (SEM) revealed the formation of a homogeneous three-dimensional TiO2 nanonetwork composed of intertwined nanofibers. X-ray photoelectron spectroscopy (XPS) confirmed the oxidative reconstruction of the Ti alloy surface. The fraction of Ti4+ species characteristic of TiO2 increased markedly from 44.2 at% to 92.2 at%, accompanied by a strong reduction in Ti0 (from 40.2 at% to 5.8 at%) and Ti3+ (from 15.7 at% to 2.1 at%). Concomitantly, lattice oxygen associated with Ti\u2013O\u2013Ti bonding increased from 48 at% to 78 at% as deduced from the O 1s signal, while the surface carbon content decreased from 48 at% to 18 at%. The modification induced a pronounced increase in surface hydrophilicity, with the water contact angle decreasing from 85\u00b0 to 32\u00b0 and the surface free energy increasing from 40.8 mJ\/m2 to 69.8 mJ\/m2. In vitro studies demonstrated good cytocompatibility and enhanced osteogenic differentiation of human mesenchymal stem cells, with twice as much alkaline phosphatase activity after 14 days and mineralization of the extracellular matrix after 28 days than those on TCPS, and also significantly higher than those on the nonmodified Ti alloy control. These findings indicate that the generated three-dimensional TiO2 nanonetwork acts as a surface-confined nanoscaffold providing nanoscale cues that promote osteogenic cell responses on titanium implant surfaces.<\/jats:p>","DOI":"10.3390\/jfb17060274","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T17:11:01Z","timestamp":1780333861000},"page":"274","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alkaline Ozonation-Induced TiO2 Nanoscaffold on Titanium Alloy for Surface-Mediated Osteogenic Guidance"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3568-2473","authenticated-orcid":false,"given":"Mariusz","family":"Winiecki","sequence":"first","affiliation":[{"name":"Department of Constructional Materials and Biomaterials, Faculty of Materials Engineering, Kazimierz Wielki University, 30 J.K. Chodkiewicza Street, 85-064 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6083-9316","authenticated-orcid":false,"given":"Piotr","family":"Krawczyk","sequence":"additional","affiliation":[{"name":"Institute of Chemistry and Technical Electrochemistry, Faculty of Chemical Technology, Poznan University of Technology, 4 Berdychowo Street, 61-131 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Reczy\u0144ska-Kolman","sequence":"additional","affiliation":[{"name":"Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Krakow, 30 A. Mickiewicza Avenue, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1024-9374","authenticated-orcid":false,"given":"Iwona","family":"Pude\u0142ko-Pra\u017cuch","sequence":"additional","affiliation":[{"name":"Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Krakow, 30 A. Mickiewicza Avenue, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0464-6189","authenticated-orcid":false,"given":"El\u017cbieta","family":"Pamu\u0142a","sequence":"additional","affiliation":[{"name":"Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Krakow, 30 A. Mickiewicza Avenue, 30-059 Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Trzcinski","sequence":"additional","affiliation":[{"name":"Division of Surface Science, Faculty of Chemical Technology and Engineering, Bydgoszcz University of Science and Technology, 7 Prof. S. Kaliskiego Avenue, 85-796 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"101745","DOI":"10.1016\/j.isci.2020.101745","article-title":"Titanium for Orthopedic Applications: An Overview of Surface Modification to Improve Biocompatibility and Prevent Bacterial Biofilm Formation","volume":"23","author":"Quinn","year":"2020","journal-title":"iScience"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100009","DOI":"10.1016\/j.nxbio.2025.100009","article-title":"A critical review on multifunctional surface strategies for titanium alloys in bioactive and durable implants","volume":"1","author":"Murugan","year":"2025","journal-title":"Next Bioeng."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Tuikampee, S., Chaijareenont, P., Rungsiyakull, P., and Yavirach, A. (2024). 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