{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T16:08:26Z","timestamp":1784822906566,"version":"3.55.0"},"reference-count":32,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Funct. Biomater.","JFB"],"abstract":"<jats:p>Acellular bone matrix, with its natural extracellular matrix components, has been considered a potential alternative platform for bone grafting. Our study focused on fabricating acellular bovine bone matrix (ABBM) and evaluating its in vitro characteristics and in vivo effect on bone repair. The bovine cancellous bone was subjected to ABBM preparation, which included partial demineralization and decellularization processing. The effects of the ABBM on human bone marrow-derived stem cells (hBMSCs) were evaluated, including viability, migration, attachment, and proliferation. A rabbit bone defect model was implanted with ABBM and histologically assessed for bone healing. The acellular properties were determined by the absence of nuclear material and the accepted minimum residual DNA content. An in vitro study indicated the ABBM\u2019s positive effect on the migration of hBMSCs. ABBM was also demonstrated to support hBMSC attachment and proliferation. In vivo testing was performed in rabbits with a cranial bone defect, which showed complete bone healing after 8 weeks of grafting with ABBM. Overall, the fabricated ABBM demonstrated in vitro and in vivo biocompatibility and effective support for bone healing in vivo, and therefore represents a potential xenogeneic biomaterial for bone tissue repair.<\/jats:p>","DOI":"10.3390\/jfb17070330","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T16:20:39Z","timestamp":1783354839000},"page":"330","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Partially Demineralized Acellular Bovine Bone Matrix Supports for Bone Healing In Vivo"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0397-7822","authenticated-orcid":false,"given":"Cuc","family":"Bui","sequence":"first","affiliation":[{"name":"Faculty of Odonto\u2014Stomatology, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Vietnam"},{"name":"Asian Cosmetic Dentistry, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan Minh","family":"To","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City 700000, Vietnam"},{"name":"Vietnam National University, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2020-4962","authenticated-orcid":false,"given":"My Thi Ngoc","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City 700000, Vietnam"},{"name":"Vietnam National University, Ho Chi Minh City 700000, Vietnam"},{"name":"Laboratory of Tissue Engineering and Biomedical Materials, University of Science, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thuan Minh","family":"Le","sequence":"additional","affiliation":[{"name":"Cantho Central General Hospital, Can Tho 900000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Triet Minh","family":"Tran","sequence":"additional","affiliation":[{"name":"Cantho Central General Hospital, Can Tho 900000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1393-0314","authenticated-orcid":false,"given":"Lam Nguyen","family":"Le","sequence":"additional","affiliation":[{"name":"Faculty of Odonto\u2014Stomatology, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc Hoang Minh","family":"Bui","sequence":"additional","affiliation":[{"name":"Faculty of Odonto\u2014Stomatology, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lam Van","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5848-1124","authenticated-orcid":false,"given":"Ha Le Bao","family":"Tran","sequence":"additional","affiliation":[{"name":"Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City 700000, Vietnam"},{"name":"Vietnam National University, Ho Chi Minh City 700000, Vietnam"},{"name":"Laboratory of Tissue Engineering and Biomedical Materials, University of Science, Ho Chi Minh City 700000, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Heller, U., Evrard, R., Lengel\u00e9, B., Schubert, T., Kadlub, N., and Boisson, J. 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