{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:55:39Z","timestamp":1791644139996,"version":"4.3.4"},"reference-count":34,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"Erasmus+ KA220-HED","doi-asserted-by":"publisher","award":["2024-1-PL01-KA220-HED-000251208"],"award-info":[{"award-number":["2024-1-PL01-KA220-HED-000251208"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009687","name":"Wroclaw Medical University","doi-asserted-by":"crossref","award":["SUBZ.B040.26.057"],"award-info":[{"award-number":["SUBZ.B040.26.057"]}],"id":[{"id":"10.13039\/501100009687","id-type":"DOI","asserted-by":"crossref"}]},{"award":["SUBZ.B040.26.057"],"award-info":[{"award-number":["SUBZ.B040.26.057"]}],"id":[{"id":"https:\/\/ror.org\/01qpw1b93","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["2024-1-PL01-KA220-HED-000251208"],"award-info":[{"award-number":["2024-1-PL01-KA220-HED-000251208"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2024-1-PL01-KA220-HED-000251208"],"award-info":[{"award-number":["2024-1-PL01-KA220-HED-000251208"]}],"id":[{"id":"https:\/\/ror.org\/019w4f821","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Funct. Biomater.","JFB"],"abstract":"<jats:p>Background: Peri-implantitis is a chronic condition that reflects an interaction between local tissue destruction, systemic inflammation and corrosion-related implant alterations. This preliminary prospective exploratory clinical study aimed to characterize the systemic cytokine profile of patients with advanced peri-implantitis requiring explantation and to explore associations between clinical severity, cytokine behavior and scanning electron microscopy\/energy-dispersive X-ray spectroscopy (SEM\/EDX)-defined corrosion-related findings. Methods: Thirteen patients with active advanced peri-implantitis were included. Probing depth and cone-beam computed tomography (CBCT)-based bone loss were assessed at four implant surfaces. Peripheral blood was collected before and 4 weeks after explantation. Serum interleukin-1 beta (IL-1\u03b2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), interleukin-12p70 (IL-12p70), tumor necrosis factor alpha (TNF-\u03b1), high-sensitivity C-reactive protein (hsCRP), and fibrinogen were analyzed. Explanted implants underwent SEM\/EDX assessment and were classified according to the presence or absence of SEM\/EDX-defined corrosion-related findings. Results: IL-6 showed the strongest and most consistent associations with clinical and radiographic severity, whereas hsCRP and fibrinogen were not similarly related. Cytokines did not show a uniform post-explantation decrease after explantation. SEM\/EDX-defined corrosion-related findings were not associated with higher baseline cytokines, but exploratory subgroup analysis suggested a divergent TNF-\u03b1 trajectory. Conclusions: These findings suggest that IL-6 may reflect the clinical inflammatory burden of advanced peri-implantitis, while corrosion-related findings may be associated with post-explantation TNF-\u03b1 behavior. Larger controlled studies are required.<\/jats:p>","DOI":"10.3390\/jfb17080395","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T09:11:16Z","timestamp":1786439476000},"page":"395","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinical Severity, Pre- and Post-Explantation Cytokine Profile and SEM\/EDX-Defined Corrosion-Related Findings in Advanced Peri-Implantitis: Preliminary Results from the PERI-EDU Prospective Exploratory Clinical Study"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8943-0549","authenticated-orcid":false,"given":"Marzena","family":"Dominiak","sequence":"first","affiliation":[{"name":"Department of Oral Surgery, Wroclaw Medical University, 50-425 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"Baranowska","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Wroclaw Medical University, 50-425 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3801-0218","authenticated-orcid":false,"given":"Jacek","family":"Matys","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Wroclaw Medical University, 50-425 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artur","family":"Pitu\u0142aj","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Wroclaw Medical University, 50-425 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Stercza\u0142a","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Wroclaw Medical University, 50-425 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9669-3187","authenticated-orcid":false,"given":"Eduard","family":"Valmaseda-Castell\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Odonto-Stomatology, Faculty of Medicine and Health Sciences, University of Barcelona, 08907 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7184-5965","authenticated-orcid":false,"given":"Ricardo Castro","family":"Alves","sequence":"additional","affiliation":[{"name":"Egas Moniz Center for Interdisciplinary Research (CiiEM), Egas Moniz School of Health & Science, 2829-511 Almada, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2564-530X","authenticated-orcid":false,"given":"Piero","family":"Papi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2439-2187","authenticated-orcid":false,"given":"Umberto","family":"Romeo","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2648-5523","authenticated-orcid":false,"given":"Wojciech","family":"Simka","sequence":"additional","affiliation":[{"name":"Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5849-7265","authenticated-orcid":false,"given":"Artur","family":"Maciej","sequence":"additional","affiliation":[{"name":"Department of Inorganic Chemistry, Analytical Chemistry and Electrochemistry, Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Kensy","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry and Preclinical Dentistry, Wroclaw Medical University, 50-425 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawe\u0142","family":"Kubasiewicz-Ross","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Wroclaw Medical University, 50-425 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Scarano, A., Khater, A.G.A., Gehrke, S.A., Serra, P., Inchingolo, F., Di Carmine, M., Tari, S.R., Leo, L., and Lorusso, F. (2023). Current Status of Peri-Implant Diseases: A Clinical Review for Evidence-Based Decision Making. J. Funct. Biomater., 14.","DOI":"10.3390\/jfb14040210"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S246","DOI":"10.1111\/jcpe.12954","article-title":"Peri-implantitis","volume":"45","author":"Schwarz","year":"2018","journal-title":"J. Clin. Periodontol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ting, M., and Suzuki, J.B. (2024). Peri-Implantitis. Dent. J., 12.","DOI":"10.3390\/dj12080251"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1177\/0022034515616557","article-title":"Increasing Prevalence of Peri-implantitis: How Will We Manage?","volume":"95","author":"Tarnow","year":"2016","journal-title":"J. Dent. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S158","DOI":"10.1111\/jcpe.12334","article-title":"Peri-implant health and disease. A systematic review of current epidemiology","volume":"42","author":"Derks","year":"2015","journal-title":"J. Clin. Periodontol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jdent.2017.04.011","article-title":"Prevalences of peri-implantitis and peri-implant mucositis: Systematic review and meta-analysis","volume":"62","author":"Lee","year":"2017","journal-title":"J. Dent."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Diaz, P., Gonzalo, E., Villagra, L.J.G., Miegimolle, B., and Suarez, M.J. (2022). What is the prevalence of peri-implantitis? A systematic review and meta-analysis. BMC Oral Health, 22.","DOI":"10.1186\/s12903-022-02493-8"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Yan, Y., Orlandi, M., Suvan, J., Harden, S., Smith, J., and D\u2019aIuto, F. (2023). Association between peri-implantitis and systemic inflammation: A systematic review. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1235155"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.1111\/clr.14339","article-title":"The systemic impact of non-surgical treatment of peri-implantitis with or without adjunctive systemic metronidazole: Secondary analysis of a randomized clinical trial","volume":"35","author":"Dopico","year":"2024","journal-title":"Clin. Oral Implants Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.1177\/00220345990780091201","article-title":"Effect of fluoride concentration and pH on corrosion behavior of titanium for dental use","volume":"78","author":"Nakagawa","year":"1999","journal-title":"J. Dent. Res."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Soler, M.D., Hsu, S.M., Fares, C., Ren, F., Jenkins, R.J., Gonzaga, L., Clark, A.E., O\u2019Neill, E., Neal, D., and Esquivel-Upshaw, J.F. (2020). Titanium Corrosion in Peri-Implantitis. Materials, 13.","DOI":"10.3390\/ma13235488"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/s41368-023-00256-x","article-title":"Titanium particles in peri-implantitis: Distribution, pathogenesis and prospects","volume":"15","author":"Chen","year":"2023","journal-title":"Int. J. Oral Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"145","DOI":"10.11005\/jbm.2022.29.3.145","article-title":"The Role of Titanium Particles and Ions in the Pathogenesis of Peri-Implantitis","volume":"29","author":"Thomsen","year":"2022","journal-title":"J. Bone Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jdsr.2021.09.001","article-title":"Impact of tribocorrosion and titanium particles release on dental implant complications\u2014A narrative review","volume":"57","author":"Kheder","year":"2021","journal-title":"Jpn. Dent. Sci. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Kheder, W., Bouzid, A., Venkatachalam, T., Talaat, I.M., Elemam, N.M., Raju, T.K., Sheela, S., Jayakumar, M.N., Maghazachi, A.A., and Samsudin, A.R. (2023). Titanium Particles Modulate Lymphocyte and Macrophage Polarization in Peri-Implant Gingival Tissues. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241411644"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/j.joms.2022.10.019","article-title":"Do Titanium Dioxide Particles Stimulate Macrophages to Release Proinflammatory Cytokines and Increase the Risk for Peri-implantitis?","volume":"81","author":"Stolzer","year":"2023","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1111\/jcpe.13836","article-title":"Surface decontamination of explanted peri-implantitis-affected implants","volume":"50","author":"Ichioka","year":"2023","journal-title":"J. Clin. Periodontol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"361","DOI":"10.2105\/AJPH.94.3.361","article-title":"Improving the reporting quality of nonrandomized evaluations of behavioral and public health interventions: The TREND statement","volume":"94","author":"Lyles","year":"2004","journal-title":"Am. J. Public Health"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1111\/clr.14059","article-title":"Effect of laser-assisted reconstructive surgical therapy of peri-implantitis on protein biomarkers and bacterial load","volume":"34","author":"Wang","year":"2023","journal-title":"Clin. Oral Implants Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"434010","DOI":"10.1155\/2013\/434010","article-title":"Cytokine levels in the serum of healthy subjects","volume":"2013","author":"Kleiner","year":"2013","journal-title":"Mediat. Inflamm."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/j.cyto.2013.09.018","article-title":"Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays","volume":"64","author":"Todd","year":"2013","journal-title":"Cytokine"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1672","DOI":"10.1111\/j.1532-5415.2009.02426.x","article-title":"Predictors of interleukin-6 elevation in older adults","volume":"57","author":"Zhu","year":"2009","journal-title":"J. Am. Geriatr. Soc."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"D\u2019Esposito, V., Di Tolla, M.F., Lecce, M., Cavalli, F., Libutti, M., Misso, S., Cabaro, S., Ambrosio, M.R., Parascandolo, A., and Covelli, B. (2022). Lifestyle and dietary habits affect plasma levels of specific cytokines in healthy subjects. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.913176"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.jobcr.2025.01.015","article-title":"Evaluating the role of inflammatory biomarkers as a diagnostic tool in peri-implantitis","volume":"15","author":"Saxena","year":"2025","journal-title":"J. Oral Biol. Craniofac. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1111\/jcpe.12586","article-title":"Peri-implant crevicular fluid biomarkers as discriminants of peri-implant health and disease","volume":"43","author":"Zani","year":"2016","journal-title":"J. Clin. Periodontol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1111\/clr.13725","article-title":"Intra-individual cytokine profile in peri-implantitis and periodontitis: A cross-sectional study","volume":"32","author":"Jansson","year":"2021","journal-title":"Clin. Oral Implants Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1902\/jop.2016.160524","article-title":"Increased Levels of Dissolved Titanium Are Associated with Peri-Implantitis: A Cross-Sectional Study","volume":"88","author":"Safioti","year":"2017","journal-title":"J. Periodontol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1111\/clr.12718","article-title":"Metal elements in tissue with dental peri-implantitis: A pilot study","volume":"27","author":"Fretwurst","year":"2016","journal-title":"Clin. Oral Implants Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1902\/jop.2014.140363","article-title":"Foreign bodies associated with peri-implantitis human biopsies","volume":"86","author":"Wilson","year":"2015","journal-title":"J. Periodontol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"121","DOI":"10.5114\/jos.2025.151575","article-title":"Relationship between toothpaste brushing, surface roughness, and bacterial adherence using contemporary tooth-colored restorative materials: A laboratory study","volume":"78","author":"Sharma","year":"2025","journal-title":"J. Stomatol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1111\/clr.13928","article-title":"Study on the immunopathological effect of titanium particles in peri-implantitis granulation tissue: A case-control study","volume":"33","author":"Rakic","year":"2022","journal-title":"Clin. Oral Implants Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"209","DOI":"10.5114\/jos.2025.154504","article-title":"Nano-structured coatings in dentistry: Surface analysis comparison of zinc oxide-coated stainless steel and zirconia crowns for pediatric use","volume":"78","author":"Jalan","year":"2025","journal-title":"J. Stomatol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"93","DOI":"10.5114\/jos.2024.139890","article-title":"Investigation of cytotoxicity and antibacterial effect of boron-containing nano-hydroxyapatite","volume":"77","author":"Demir","year":"2024","journal-title":"J. Stomatol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Guler, B., Uraz, A., Hatipo\u011flu, H., and Yal\u0131m, M. (2021). Chemical Evaluation of Energy Dispersive X-ray Spectroscopy Analysis of Different Failing Dental Implant Surfaces: A Comparative Clinical Trial. Materials, 14.","DOI":"10.3390\/ma14040986"}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/8\/395\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T04:24:04Z","timestamp":1786595044000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/8\/395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,11]]},"references-count":34,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["jfb17080395"],"URL":"https:\/\/doi.org\/10.3390\/jfb17080395","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,11]]}}}