{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T12:32:25Z","timestamp":1787056345470,"version":"build-2736575974"},"reference-count":44,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004488","name":"Croatian Science Foundation","doi-asserted-by":"publisher","award":["HRZZ-IP-2022-10-6065"],"award-info":[{"award-number":["HRZZ-IP-2022-10-6065"]}],"id":[{"id":"10.13039\/501100004488","id-type":"DOI","asserted-by":"publisher"}]},{"award":["HRZZ-IP-2022-10-6065"],"award-info":[{"award-number":["HRZZ-IP-2022-10-6065"]}],"id":[{"id":"https:\/\/ror.org\/03n51vw80","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Funct. Biomater.","JFB"],"abstract":"<jats:p>The aim of this study was to evaluate the influence of n-butyl cyanoacrylate (NBCA) coating of freshly mixed mineral trioxide aggregate (MTA) on its microhardness after setting under conditions of moisture and human blood exposure. MicroMega MM-MTA samples were prepared using Teflon molds (6 mm \u00d7 4 mm). Four experimental groups (n = 4) were established: control (MTA + phosphate-buffered saline (PBS)), MTA coated with NBCA (PeriAcryl) + PBS, MTA + blood + PBS, and MTA coated with NBCA + blood + PBS. Samples assigned to the blood-exposure groups were exposed to human blood for 15 min, rinsed, and subsequently stored in PBS. Prior to microhardness testing, all samples were incubated in PBS for seven days. Microhardness was measured using a Vickers microhardness tester, with five indentations performed on each specimen (20 measurements per group). Data were analyzed using the Shapiro\u2013Wilk test for normality, Levene\u2019s test, and two-way ANOVA, followed by Tukey\u2019s post hoc test. The analysis revealed significant differences among the experimental groups (p &lt; 0.001). Post hoc analysis demonstrated statistically significant differences between tissue glue-coated and uncoated samples (p &lt; 0.05). Within the limitations of this in vitro study, coating MTA with NBCA tissue glue during the setting period significantly increased its microhardness following exposure to human blood and PBS.<\/jats:p>","DOI":"10.3390\/jfb17080410","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T16:35:08Z","timestamp":1786984508000},"page":"410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of n-Butyl Cyanoacrylate Coating on the Microhardness of MM-MTA After Exposure to Moisture and Human Blood"],"prefix":"10.3390","volume":"17","author":[{"given":"Jurica","family":"Matijevi\u0107","sequence":"first","affiliation":[{"name":"University of Zagreb School of Dental Medicine, Department of Endodontics and Restorative Dentistry, Gunduli\u0107eva 5, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9117-3972","authenticated-orcid":false,"given":"Anja","family":"Baraba","sequence":"additional","affiliation":[{"name":"University of Zagreb School of Dental Medicine, Department of Endodontics and Restorative Dentistry, Gunduli\u0107eva 5, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4176-7480","authenticated-orcid":false,"given":"Adna","family":"Vlahovljak Feru\u0161i\u0107","sequence":"additional","affiliation":[{"name":"Private Dental Office Adenta, Bulevar Me\u0161e Selimovi\u0107a 2C\/3, 71000 Sarajevo, Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matea","family":"Vidov","sequence":"additional","affiliation":[{"name":"Private Dental Office, Vivantadental, Avenida del Doctor Peset Aleixandre 142, 46002 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoran","family":"Karlovi\u0107","sequence":"additional","affiliation":[{"name":"University of Zagreb School of Dental Medicine, Department of Endodontics and Restorative Dentistry, Gunduli\u0107eva 5, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1380-0029","authenticated-orcid":false,"given":"Ana","family":"Ivani\u0161evi\u0107","sequence":"additional","affiliation":[{"name":"University of Zagreb School of Dental Medicine, Department of Endodontics and Restorative Dentistry, Gunduli\u0107eva 5, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/S0099-2399(06)81282-3","article-title":"Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations","volume":"19","author":"Lee","year":"1993","journal-title":"J. Endod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1016\/j.joen.2009.09.009","article-title":"Mineral trioxide aggregate: A comprehensive literature review\u2014Part III: Clinical applications, drawbacks, and mechanism of action","volume":"36","author":"Parirokh","year":"2010","journal-title":"J. Endod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1111\/iej.12843","article-title":"Mineral trioxide aggregate and other bioactive endodontic cements: An updated overview\u2014Part II: Other clinical applications and complications","volume":"51","author":"Torabinejad","year":"2018","journal-title":"Int. Endod. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.joen.2015.12.022","article-title":"A review of tooth discoloration after regenerative endodontic therapy","volume":"42","author":"Kahler","year":"2016","journal-title":"J. Endod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e20170465","DOI":"10.1590\/1678-7757-2017-0465","article-title":"Solubility, porosity and fluid uptake of calcium silicate-based cements","volume":"26","author":"Torres","year":"2018","journal-title":"J. Appl. Oral Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3839","DOI":"10.1007\/s00784-023-05002-5","article-title":"Biocompatibility, bioactive potential, porosity, and interface analysis of calcium silicate repair cements in a dentin tube model","volume":"27","author":"Inada","year":"2023","journal-title":"Clin. Oral Investig."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Sanz, J.L., Forner, L., Llena, C., Guerrero-Giron\u00e9s, J., Melo, M., Rengo, S., Spagnuolo, G., and Rodr\u00edguez-Lozano, F.J. (2020). Cytocompatibility and bioactive properties of hydraulic calcium silicate-based cements (HCSCs) on stem cells from human exfoliated deciduous teeth (SHEDs): A systematic review of in vitro studies. J. Clin. Med., 9.","DOI":"10.3390\/jcm9123872"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1111\/j.1365-2591.2010.01745.x","article-title":"The effect of blood contamination on the compressive strength and surface microstructure of mineral trioxide aggregate","volume":"43","author":"Nekoofar","year":"2010","journal-title":"Int. Endod. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1111\/j.1365-2591.2010.01750.x","article-title":"An evaluation of the effect of blood and human serum on the surface microhardness and surface microstructure of mineral trioxide aggregate","volume":"43","author":"Nekoofar","year":"2010","journal-title":"Int. Endod. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.joen.2005.09.007","article-title":"Effect of blood contamination on retention characteristics of MTA when mixed with different liquids","volume":"32","author":"Vanderweele","year":"2006","journal-title":"J. Endod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"273","DOI":"10.2334\/josnusd.50.273","article-title":"Comparison of microleakage with three different thicknesses of mineral trioxide aggregate as a root-end filling material","volume":"50","author":"Rahimi","year":"2008","journal-title":"J. Oral Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/j.joen.2008.07.017","article-title":"Effect of pH on sealing ability of white mineral trioxide aggregate as a root-end filling material","volume":"34","author":"Saghiri","year":"2008","journal-title":"J. Endod."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e1014","DOI":"10.4317\/medoral.17158","article-title":"Effect of alkaline pH on sealing ability of white mineral trioxide aggregate","volume":"16","author":"Lotfi","year":"2011","journal-title":"Med. Oral Patol. Oral Cir. Bucal"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"187","DOI":"10.2334\/josnusd.51.187","article-title":"Effect of phosphate-buffered saline on coronal leakage of mineral trioxide aggregate","volume":"51","author":"Parirokh","year":"2009","journal-title":"J. Oral Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1097\/01.DON.0000133155.04468.41","article-title":"Physicochemical basis of the biologic properties of mineral trioxide aggregate","volume":"31","author":"Sarkar","year":"2005","journal-title":"J. Endod."},{"key":"ref_16","first-page":"540","article-title":"Push-out bond strength of bioceramic materials in a synthetic tissue fluid","volume":"10","author":"Shokouhinejad","year":"2013","journal-title":"J. Dent."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Gerardi, D., Burdo, P., Turkyilmaz, I., Diomede, F., Mendes, G.D., Piattelli, M., and Varvara, G. (2025). Clinical applications and efficacy of cyanoacrylates in oral surgery and periodontology: A scoping review. Medicina, 61.","DOI":"10.3390\/medicina61071286"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1007\/s10856-021-06573-z","article-title":"Comparative analysis of the hemostatic, analgesic and healing effects of cyanoacrylate on free gingival graft surgical wounds in donor and recipient areas: A systematic review","volume":"32","author":"Ribeiro","year":"2021","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"110796","DOI":"10.1016\/j.msec.2020.110796","article-title":"The recent progress of tissue adhesives in design strategies, adhesive mechanism and applications","volume":"111","author":"Bao","year":"2020","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0142-9612(00)00163-0","article-title":"Cytotoxicity, blood compatibility and antimicrobial activity of two cyanoacrylate glues for surgical use","volume":"22","author":"Montanaro","year":"2001","journal-title":"Biomaterials"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e70047","DOI":"10.1111\/iwj.70047","article-title":"Tissue adhesives for closure of intraoral surgical incisions: A systematic review and meta-analysis","volume":"21","author":"Medeiros","year":"2024","journal-title":"Int. Wound J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/S0142-9612(03)00591-X","article-title":"Effects of physiological environments on the hydration behavior of mineral trioxide aggregate","volume":"25","author":"Lee","year":"2004","journal-title":"Biomaterials"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e20180093","DOI":"10.1590\/1678-7757-2018-0093","article-title":"Surface and vertical dimensional changes of mineral trioxide aggregate and Biodentine in different environmental conditions","volume":"27","author":"Aksel","year":"2018","journal-title":"J. Appl. Oral Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Shokouhinejad, N., Hosseini, P., and Razmi, H. (2024). The effect of human blood and platelet-rich fibrin on the surface microhardness of hydraulic calcium silicate-based cements. Front. Dent., 21.","DOI":"10.18502\/fid.v21i41.16847"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1111\/j.1365-2591.2007.01370.x","article-title":"Characterization of hydration products of mineral trioxide aggregate","volume":"41","author":"Camilleri","year":"2008","journal-title":"Int. Endod. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"188","DOI":"10.5395\/rde.2012.37.4.188","article-title":"Chemical characteristics of mineral trioxide aggregate and its hydration reaction","volume":"37","author":"Chang","year":"2012","journal-title":"Restor. Dent. Endod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.dental.2013.03.007","article-title":"Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus","volume":"29","author":"Camilleri","year":"2013","journal-title":"Dent. Mater."},{"key":"ref_28","first-page":"e393","article-title":"Microhardness and sealing ability of materials used for root canal perforations","volume":"60","author":"Camargo","year":"2012","journal-title":"Gen. Dent."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"319","DOI":"10.14744\/eej.2025.60024","article-title":"Evaluation of void volume and blood contamination-induced changes in surface microhardness of calcium silicate-based cement, sealer, and their combination in retrograde filling","volume":"10","author":"Kiatpattanakrai","year":"2025","journal-title":"Eur. Endodon. J."},{"key":"ref_30","unstructured":"World Health Organization (2010). WHO Guidelines on Drawing Blood: Best Practices in Phlebotomy, World Health Organization."},{"key":"ref_31","first-page":"e63632","article-title":"Comparative evaluation of the mechanical and physical properties of mineral trioxide aggregate vs. bacterial cellulose nanocrystal-reinforced mineral trioxide aggregate: An in vitro study","volume":"16","author":"Priya","year":"2024","journal-title":"Cureus"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"106078","DOI":"10.1016\/j.jmbbm.2023.106078","article-title":"Effect of incorporation of nano-graphene oxide on physicochemical, mechanical, and biological properties of tricalcium silicate cement","volume":"146","author":"Somaie","year":"2023","journal-title":"J. Mech. Behav. Biomed. Mater."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Patri, G., Bansal, S., and Lath, H. (BMC Oral Health, 2026). Physicochemical and mechanical properties of gallium oxide-, cerium oxide-, and graphene oxide-modified mineral trioxide aggregate: An in vitro study, BMC Oral Health, in press.","DOI":"10.1186\/s12903-026-08621-y"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1590\/S1806-83242008000100008","article-title":"In vivo qualitative analysis of the biocompatibility of different cyanoacrylate-based adhesives","volume":"22","author":"Mello","year":"2008","journal-title":"Braz. Oral Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1590\/S0103-64402006000400011","article-title":"Sealer penetration and marginal permeability after apicoectomy varying retrocavity preparation and retrofilling material","volume":"17","author":"Winik","year":"2006","journal-title":"Braz. Dent. J."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Yousefi-Koma, A.A., Assadian, H., Mohaghegh, S., and Nokhbatolfoghahaei, H. (2023). Comparative biocompatibility and odonto-\/osteogenesis effects of hydraulic calcium silicate-based cements in simulated direct and indirect approaches for regenerative endodontic treatments: A systematic review. J. Funct. Biomater., 14.","DOI":"10.3390\/jfb14090446"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.jdent.2013.12.015","article-title":"A review of the bioactivity of hydraulic calcium silicate cements","volume":"42","author":"Niu","year":"2014","journal-title":"J. Dent."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Falkowska, J., Chady, T., Dura, W., Dro\u017adzik, A., Tomasik, M., Marek, E., Safranow, K., and Lipski, M. (2023). The washout resistance of bioactive root-end filling materials. Materials, 16.","DOI":"10.3390\/ma16175757"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Comby, P.-O., Guillen, K., Chevallier, O., Lenfant, M., Pellegrinelli, J., Falvo, N., Midulla, M., and Loffroy, R. (2021). Endovascular use of cyanoacrylate-lipiodol mixture for peripheral embolization: Properties, techniques, pitfalls, and applications. J. Clin. Med., 10.","DOI":"10.3390\/jcm10194320"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e14","DOI":"10.1016\/j.tripleo.2006.05.001","article-title":"Biochemical and histopathological findings of N-butyl-2-cyanoacrylate in oral surgery: An experimental study","volume":"102","author":"Inal","year":"2006","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"633","DOI":"10.4103\/0970-9290.107381","article-title":"The effectiveness of using different pulp-capping agents on the healing response of the pulp","volume":"23","author":"Aljandan","year":"2012","journal-title":"Indian J. Dent. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1111\/iej.13128","article-title":"Management of deep caries and the exposed pulp","volume":"52","author":"Simon","year":"2019","journal-title":"Int. Endod. J."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Pushpalatha, C., Dhareshwar, V., Sowmya, S.V., Augustine, D., Vinothkumar, T.S., Renugalakshmi, A., Shaiban, A., Kakti, A., Bhandi, S.H., and Dubey, A. (2022). Modified Mineral Trioxide Aggregate\u2014A Versatile Dental Material: An Insight on Applications and Newer Advancements. Front. Bioeng. Biotechnol., 10.","DOI":"10.3389\/fbioe.2022.941826"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1542","DOI":"10.1016\/j.dental.2026.03.165","article-title":"Highly alkaline mineral trioxide aggregate (MTA) affects restorative materials","volume":"42","author":"Yoshihara","year":"2026","journal-title":"Dent. Mater."}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/8\/410\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T12:23:02Z","timestamp":1787055782000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/8\/410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,17]]},"references-count":44,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["jfb17080410"],"URL":"https:\/\/doi.org\/10.3390\/jfb17080410","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,17]]}}}