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Funct. Biomater.","JFB"],"abstract":"<jats:p>Introduction: The clinical translation of biomaterial-based strategies for ear reconstruction remains limited by several challenges, including scaffold design, selection of an optimal cell source, and stability of the new cartilage tissue. Most recent studies still rely on animal-derived cells and fail to demonstrate the long-term maintenance of the chondrogenic phenotype. Methods: In this study, we developed an off-the-shelf poly(lactide-b-ethylene glycol) (PLA-PEG) scaffold combined with clinical-grade alginate (Alg) and cellularized with human nasal chondrocytes for auricular cartilage engineering. Cellularized constructs were evaluated using histological and immunofluorescence analyses to assess extracellular matrix production and quality. In parallel, constructs retrieved after 12 weeks of in vivo implantation were mechanically characterized using a UniVert CellScale testing system to determine variations in compressive strength. Finally, the chondrogenic maturation of the new tissue was evaluated through bulk RNA transcriptomic profiling. Results: Histological, immunofluorescence, and transcriptomic analyses demonstrated robust cartilage matrix deposition with improved compressive properties and high expression of chondrogenic markers, such as ACAN, PRG4, and COL2A1. Furthermore, positive staining for the human-specific Ku80 antibody confirmed the presence of cells of human origin in the newly formed tissue after implantation. Conclusions: Overall, this study provides evidence that the combination of a PLA-PEG + Alg scaffold with human nasal septal chondrocytes represents a promising strategy for future reconstructive applications in auricular tissue engineering.<\/jats:p>","DOI":"10.3390\/jfb17090453","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T10:30:46Z","timestamp":1788777046000},"page":"453","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cellularized Thermoformed Scaffolds with Human Septal Chondrocytes Support In Vivo Cartilage Maturation for Auricular Reconstruction"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6618-4135","authenticated-orcid":false,"given":"Emma","family":"Mui\u00f1os-L\u00f3pez","sequence":"first","affiliation":[{"name":"Experimental Orthopedics Laboratory, Department of Orthopedic Surgery and Traumatology, Cl\u00ednica Universidad de Navarra (CUN), P\u00edo XII, 36, 31008 Pamplona, Spain"},{"name":"Hematology and Cell Therapy Area, Cl\u00ednica Universidad de Navarra (CUN), P\u00edo XII, 36, 31008 Pamplona, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria de Navarra (IdiSNA), Irunlarrea, 3, 31008 Pamplona, Spain"},{"name":"Bioengineering Program, Technological Innovation Division, Cima Universidad de Navarra, P\u00edo XII 55, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9908-2346","authenticated-orcid":false,"given":"I\u00f1igo","family":"Arroyo","sequence":"additional","affiliation":[{"name":"Department of Plastic Aesthetic and Reconstructive Surgery, Cl\u00ednica Universidad de Navarra (CUN), P\u00edo XII, 36, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9166-5865","authenticated-orcid":false,"given":"Olatz","family":"Guaresti","sequence":"additional","affiliation":[{"name":"Leartiker S. 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Am. Acad. Dermatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101141","DOI":"10.1016\/j.mtbio.2024.101141","article-title":"3D printing tissue-engineered scaffolds for auricular reconstruction","volume":"27","author":"Gao","year":"2024","journal-title":"Mater. Today Bio"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1177\/229255031402200102","article-title":"Auricular reconstruction for microtia: A review of available methods","volume":"22","author":"Baluch","year":"2014","journal-title":"Plast. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Federspil, P.A. (2010). Auricular Prostheses, Karger Publishers.","DOI":"10.1159\/000314563"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1097\/SCS.0000000000008130","article-title":"Systematic Review of Medpor Versus Autologous Ear Reconstruction","volume":"33","author":"Ma","year":"2022","journal-title":"J. Craniofac. Surg."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.fsc.2023.09.002","article-title":"Our Evolution of Approaches to Microtia Reconstruction","volume":"32","author":"Quatela","year":"2024","journal-title":"Facial Plast. Surg. Clin."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"456","DOI":"10.3349\/ymj.2023.0444","article-title":"One-Year Results of Ear Reconstruction with 3D Printed Implants","volume":"65","author":"Kim","year":"2024","journal-title":"Yonsei Med. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.cps.2024.10.002","article-title":"Microtia","volume":"52","author":"Camison","year":"2025","journal-title":"Clin. Plast. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Mussi, E., Furferi, R., Volpe, Y., Facchini, F., McGreevy, K.S., and Uccheddu, F. (2019). Ear Reconstruction Simulation: From Handcrafting to 3D Printing. Bioengineering, 6.","DOI":"10.3390\/bioengineering6010014"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s11626-997-0054-x","article-title":"Distribution and viability of cultured human chondrocytes in a three-dimensional matrix as assessed by confocal laser scan microscopy","volume":"33","author":"Aigner","year":"1997","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0142-9612(96)85561-X","article-title":"Tissue engineering and autologous transplant formation: Practical approaches with resorbable biomaterials and new cell culture techniques","volume":"17","author":"Sittinger","year":"1996","journal-title":"Biomaterials"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1089\/1076327041348527","article-title":"Tissue engineering of an auricular cartilage model utilizing cultured chondrocyte-poly(L-lactide-epsilon-caprolactone) scaffolds","volume":"10","author":"Isogai","year":"2004","journal-title":"Tissue Eng."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.1524-475X.2008.00451.x","article-title":"Tissue engineering a model for the human ear: Assessment of size, shape, morphology, and gene expression following seeding of different chondrocytes","volume":"17","author":"Kusuhara","year":"2009","journal-title":"Wound Repair Regen."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.actbio.2024.03.012","article-title":"Bioengineering Full-scale auricles using 3D-printed external scaffolds and decellularized cartilage xenograft","volume":"179","author":"Vernice","year":"2024","journal-title":"Acta Biomater."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1097\/SAP.0000000000002213","article-title":"The History of Alloplastic Ear Reconstruction for Microtia","volume":"85","author":"Kozusko","year":"2020","journal-title":"Ann. Plast. Surg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1011E","DOI":"10.1097\/PRS.0000000000011219","article-title":"Update on Total Auricular Construction","volume":"153","author":"Yamada","year":"2024","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1016\/j.biomaterials.2005.09.019","article-title":"The in vivo degradation, absorption and excretion of PCL-based implant","volume":"27","author":"Sun","year":"2006","journal-title":"Biomaterials"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1080\/10601320701424487","article-title":"Degradation Profile of Poly(\u03f5-caprolactone)\u2013the Influence of Macroscopic and Macromolecular Biomaterial Design","volume":"44","author":"Hakkarainen","year":"2007","journal-title":"J. Macromol. Sci. Part A Pure Appl. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"31","DOI":"10.2147\/CCID.S229054","article-title":"Polycaprolactone: How a Well-Known and Futuristic Polymer Has Become an Innovative Collagen-Stimulator in Esthetics","volume":"13","author":"Christen","year":"2020","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1038\/s41598-025-85169-x","article-title":"Single-Cell RNA sequencing reveals mitochondrial dysfunction in microtia chondrocytes","volume":"15","author":"Li","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"132","DOI":"10.22203\/eCM.v035a10","article-title":"Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture","volume":"35","author":"Otto","year":"2018","journal-title":"Eur. Cell. Mater."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1097\/SCS.0000000000006320","article-title":"Costal Cartilage and Costal Perichondrium Sandwich Graft in Septal Perforation Repair","volume":"31","author":"Hanci","year":"2020","journal-title":"J. Craniofac. Surg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"045028","DOI":"10.1088\/1748-605X\/ac78b7","article-title":"3D printed bioresorbable scaffolds for articular cartilage tissue engineering: A comparative study between neat polycaprolactone (PCL) and poly(lactide-b-ethylene glycol) (PLA-PEG) block copolymer","volume":"17","author":"Urtaza","year":"2022","journal-title":"Biomed. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1007\/s00266-024-04481-5","article-title":"Age and Gender Related Variability in the Strength of Auricular Cartilage in Living Humans","volume":"49","author":"Buyuklu","year":"2025","journal-title":"Aesthetic Plast. Surg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1097\/00006534-199708000-00001","article-title":"Transplantation of chondrocytes utilizing a polymer-cell construct to produce tissue-engineered cartilage in the shape of a human ear","volume":"100","author":"Cao","year":"1997","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1055\/s-0037-1604261","article-title":"Total Ear Reconstruction Using Porous Polyethylene","volume":"31","author":"Ali","year":"2017","journal-title":"Semin. Plast. Surg."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Huang, Y., Zhao, H., Wang, Y., Bi, S., Zhou, K., Li, H., Zhou, C., Wang, Y., Wu, W., and Peng, B. (2023). The application and progress of tissue engineering and biomaterial scaffolds for total auricular reconstruction in microtia. Front. Bioeng. Biotechnol., 11.","DOI":"10.3389\/fbioe.2023.1089031"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Shi, W., Chen, S., Liu, B., Omidian, H., Dey Chowdhury, S., and Wilson, R.L. (2024). Advancements and Challenges in Hydrogel Engineering for Regenerative Medicine. Gels, 10.","DOI":"10.3390\/gels10040238"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Ansari, M., Darvishi, A., and Sabzevari, A. (2024). A review of advanced hydrogels for cartilage tissue engineering. Front. Bioeng. Biotechnol., 12.","DOI":"10.3389\/fbioe.2024.1340893"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1007\/s42235-024-00480-z","article-title":"Recent Progress in Bionic Hydrogels for Articular Cartilage: Tribological and Mechanical Characteristics","volume":"21","author":"Xiong","year":"2024","journal-title":"J. Bionic Eng."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e702","DOI":"10.1002\/ctm2.702","article-title":"Single-cell transcriptomics reveals pathogenic dysregulation of previously unrecognised chondral stem\/progenitor cells in children with microtia","volume":"12","author":"Ma","year":"2022","journal-title":"Clin. Transl. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1186\/s13023-024-03149-2","article-title":"Multi-omics analysis of a case of congenital microtia reveals aldob and oxidative stress associated with microtia etiology","volume":"19","author":"Liu","year":"2024","journal-title":"Orphanet J. Rare Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1055\/s-0037-1607332","article-title":"Comparing the Host Reaction to CorMatrix and Different Cardiac Patch Materials Implanted Subcutaneously in Growing Pigs","volume":"67","author":"Poncelet","year":"2019","journal-title":"Thorac. Cardiovasc. Surg."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"13027","DOI":"10.1038\/s41598-017-13417-w","article-title":"Vascular Endothelial Growth Factor in Cartilage Development and Osteoarthritis","volume":"7","author":"Nagao","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5501","DOI":"10.1126\/sciadv.adi5501","article-title":"Nanoparticle-based inhibition of vascular endothelial growth factor receptors alleviates osteoarthritis pain and cartilage damage","volume":"10","author":"Ma","year":"2024","journal-title":"Sci. Adv."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Reinisch, J.F., and Tahiri, Y. (2019). Modern Microtia Reconstruction: Art, Science, and New Clinical Techniques, Springer.","DOI":"10.1007\/978-3-030-16387-7"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3460","DOI":"10.1007\/s10439-016-1688-1","article-title":"Biomechanical Characterisation of the Human Auricular Cartilages; Implications for Tissue Engineering","volume":"44","author":"Griffin","year":"2016","journal-title":"Ann. Biomed. Eng."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"631","DOI":"10.18063\/ijb.v9i1.631","article-title":"Bacterial nanocellulose-reinforced gelatin methacryloyl hydrogel enhances biomechanical property and glycosaminoglycan content of 3D-bioprinted cartilage","volume":"9","author":"Zeng","year":"2022","journal-title":"Int. J. Bioprinting"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e05389","DOI":"10.1002\/adhm.202505389","article-title":"Enhanced Auricular Cartilage Regeneration via 3D-Printed Hydrogel with miR-92a-3p-Enriched Platelet-Rich Plasma-Derived Extracellular Vesicles","volume":"15","author":"Hua","year":"2026","journal-title":"Adv. Healthc. Mater."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"015010","DOI":"10.1088\/1758-5090\/ac3b91","article-title":"Human-engineered auricular reconstruction (hEAR) by 3D-printed molding with human-derived auricular and costal chondrocytes and adipose-derived mesenchymal stem cells","volume":"14","author":"Landau","year":"2021","journal-title":"Biofabrication"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"eadh1890","DOI":"10.1126\/sciadv.adh1890","article-title":"Combining bioengineered human skin with bioprinted cartilage for ear reconstruction","volume":"9","author":"Zielinska","year":"2023","journal-title":"Sci. Adv."},{"key":"ref_42","first-page":"66","article-title":"Bioprinting and regeneration of auricular cartilage using a bioactive bioink based on microporous photocrosslinkable acellular cartilage matrix","volume":"16","author":"Jia","year":"2022","journal-title":"Bioact. Mater."},{"key":"ref_43","first-page":"461","article-title":"Unconventional auricular reconstruction using controlled scaffold buckling and chondrogenic cocktail containing muscle-derived cells","volume":"65","author":"Huang","year":"2026","journal-title":"Bioact. Mater."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zhang, G., Li, J., Wang, H., Shangguan, C., Xie, J., and Zhou, Y. (2025). Research on the Impact Toughness of 3D-Printed CoCrMo Alloy Components Based on Fractal Theory. Biomimetics, 10.","DOI":"10.3390\/biomimetics10050292"}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/9\/453\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T13:34:47Z","timestamp":1789133687000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/17\/9\/453"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,7]]},"references-count":44,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["jfb17090453"],"URL":"https:\/\/doi.org\/10.3390\/jfb17090453","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,7]]}}}