{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T19:41:24Z","timestamp":1760211684362,"version":"build-2065373602"},"reference-count":80,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,1,7]],"date-time":"2016-01-07T00:00:00Z","timestamp":1452124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>The present work addresses the question of to what extent a geometrical support acts as a physiological determining template in the setup of artificial cardiac tissue. Surface patterns with alternating concave to convex transitions of cell size dimensions were used to organize and orientate human-induced pluripotent stem cell (hIPSC)-derived cardiac myocytes and mouse neonatal cardiac myocytes. The shape of the cells, as well as the organization of the contractile apparatus recapitulates the anisotropic line pattern geometry being derived from tissue geometry motives. The intracellular organization of the contractile apparatus and the cell coupling via gap junctions of cell assemblies growing in a random or organized pattern were examined. Cell spatial and temporal coordinated excitation and contraction has been compared on plain and patterned substrates. While the \u03b1-actinin cytoskeletal organization is comparable to terminally-developed native ventricular tissue, connexin-43 expression does not recapitulate gap junction distribution of heart muscle tissue. However, coordinated contractions could be observed. The results of tissue-like cell ensemble organization open new insights into geometry-dependent cell organization, the cultivation of artificial heart tissue from stem cells and the anisotropy-dependent activity of therapeutic compounds.<\/jats:p>","DOI":"10.3390\/jfb7010001","type":"journal-article","created":{"date-parts":[[2016,1,7]],"date-time":"2016-01-07T11:11:51Z","timestamp":1452165111000},"page":"1","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tissue-Mimicking Geometrical Constraints Stimulate Tissue-Like Constitution and Activity of Mouse Neonatal and Human-Induced Pluripotent Stem Cell-Derived Cardiac Myocytes"],"prefix":"10.3390","volume":"7","author":[{"given":"G\u00f6tz","family":"Pilarczyk","sequence":"first","affiliation":[{"name":"Kirchhoff Institute f\u00fcr Physik, Im Neuenheimer Feld INF 270, Heidelberg D-69120, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Raulf","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologie der Unversit\u00e4t Bonn, Life &amp; Brain Center, Sigmund Freud Strasse 25, Bonn D-53127, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Gunkel","sequence":"additional","affiliation":[{"name":"ViroQuant Cell Networks RNAi Screening Facility, BioQuant Center, Im Neuenheimer Feld INF 267, Heidelberg D-69120, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Fleischmann","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologie der Unversit\u00e4t Bonn, Life &amp; Brain Center, Sigmund Freud Strasse 25, Bonn D-53127, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Lemor","sequence":"additional","affiliation":[{"name":"Luxembourg Institute for Science and Technology, 5 avenue des Hauts-Fourneaux, Esch-Belval L-4362, Luxembourg"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9430-1987","authenticated-orcid":false,"given":"Michael","family":"Hausmann","sequence":"additional","affiliation":[{"name":"Kirchhoff Institute f\u00fcr Physik, Im Neuenheimer Feld INF 270, Heidelberg D-69120, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2016,1,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.stem.2011.12.013","article-title":"Production of de novo cardiomyocytes: Human pluripotent stem cell differentiation and direct reprogramming","volume":"10","author":"Burridge","year":"2012","journal-title":"Cell Stem Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1111\/j.1755-148X.2010.00786.x","article-title":"Artificial skin perspective: Concepts and applications","volume":"24","author":"Brohem","year":"2010","journal-title":"Pigment Cell Melanoma Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1098\/rsif.2009.0403","article-title":"A review of tissue-engineered skin bioconstructs available for skin reconstruction","volume":"7","author":"Shevchenko","year":"2010","journal-title":"J. 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