{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T10:07:31Z","timestamp":1771841251172,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,9,15]],"date-time":"2018-09-15T00:00:00Z","timestamp":1536969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP-12087"],"award-info":[{"award-number":["MOP-12087"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["FDN-143213"],"award-info":[{"award-number":["FDN-143213"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFB"],"abstract":"<jats:p>There is a high incidence of failure and recurrence for chronic skin wounds following conventional therapies. To promote healing, the use of skin substitutes containing living cells as wound dressings has been proposed. The aim of this study was to produce a scaffold-free cell-based bilayered tissue-engineered skin substitute (TES) containing living fibroblasts and keratinocytes suitable for use as wound dressing, while considering production time, handling effort during the manufacturing process, and stability of the final product. The self-assembly method, which relies on the ability of mesenchymal cells to secrete and organize connective tissue sheet sustaining keratinocyte growth, was used to produce TESs. Three fibroblast-seeding densities were tested to produce tissue sheets. At day 17, keratinocytes were added onto 1 or 3 (reference method) stacked tissue sheets. Four days later, TESs were subjected either to 4, 10, or 17 days of culture at the air\u2013liquid interface (A\/L). All resulting TESs were comparable in terms of their histological aspect, protein expression profile and contractile behavior in vitro. However, signs of extracellular matrix (ECM) digestion that progressed over culture time were noted in TESs produced with only one fibroblast-derived tissue sheet. With lower fibroblast density, the ECM of TESs was almost completely digested after 10 days A\/L and lost histological integrity after grafting in athymic mice. Increasing the fibroblast seeding density 5 to 10 times solved this problem. We conclude that the proposed method allows for a 25-day production of a living TES, which retains its histological characteristics in vitro for at least two weeks.<\/jats:p>","DOI":"10.3390\/jfb9030053","type":"journal-article","created":{"date-parts":[[2018,9,17]],"date-time":"2018-09-17T10:42:20Z","timestamp":1537180940000},"page":"53","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Specialized Living Wound Dressing Based on the Self-Assembly Approach of Tissue Engineering"],"prefix":"10.3390","volume":"9","author":[{"given":"Laurence","family":"Cantin-Warren","sequence":"first","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, D\u00e9partement de Chirurgie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, 1401 18e Rue, Qu\u00e9bec, Qu\u00e9bec G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rina","family":"Guignard","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, D\u00e9partement de Chirurgie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, 1401 18e Rue, Qu\u00e9bec, Qu\u00e9bec G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Cortez Ghio","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, D\u00e9partement de Chirurgie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, 1401 18e Rue, Qu\u00e9bec, Qu\u00e9bec G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle","family":"Larouche","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, D\u00e9partement de Chirurgie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, 1401 18e Rue, Qu\u00e9bec, Qu\u00e9bec G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois A.","family":"Auger","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, D\u00e9partement de Chirurgie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, 1401 18e Rue, Qu\u00e9bec, Qu\u00e9bec G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8883-6491","authenticated-orcid":false,"given":"Lucie","family":"Germain","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Organog\u00e9n\u00e8se Exp\u00e9rimentale de l\u2019Universit\u00e9 Laval\/LOEX, Regenerative Medicine Division, CHU de Qu\u00e9bec-Universit\u00e9 Laval Research Centre, D\u00e9partement de Chirurgie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval, 1401 18e Rue, Qu\u00e9bec, Qu\u00e9bec G1J 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/j.1524-475X.2009.00543.x","article-title":"Human skin wounds: A major and snowballing threat to public health and the economy","volume":"17","author":"Sen","year":"2009","journal-title":"Wound Repair Regen."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhou, K., Krug, K., and Brogan, M.S. 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Part A"}],"container-title":["Journal of Functional Biomaterials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-4983\/9\/3\/53\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:20:42Z","timestamp":1760196042000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-4983\/9\/3\/53"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,15]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,9]]}},"alternative-id":["jfb9030053"],"URL":"https:\/\/doi.org\/10.3390\/jfb9030053","relation":{},"ISSN":["2079-4983"],"issn-type":[{"value":"2079-4983","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,15]]}}}