{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:22:01Z","timestamp":1760239321905,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T00:00:00Z","timestamp":1604707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JFMK"],"abstract":"<jats:p>A unilateral posterior crossbite is a malocclusion where the low activity of the affected masseter muscle is compensated by the contralateral muscle hypertrophy. It is still unknown if, in the same condition, myogenesis with new fibre formation takes place. Aim: the aim of the present study was to evaluate the expression of myogenesis markers, such as Myf5 and MyoD, in masseter muscles of unilateral posterior crossbite patients. Materials and methods: biopsies from fifteen surgical patients with unilateral posterior crossbites have been analysed by immunofluorescence reactions. The results show the expression of Myf5 and MyoD in the contralateral muscle but not in the ipsilateral one. Moreover, statistical analysis shows the higher number of satellite cells in the contralateral side if compared to the ipsilateral one. Conclusions: these results suggest that in contralateral muscle, hyperplastic events take place, as well as hypertrophy.<\/jats:p>","DOI":"10.3390\/jfmk5040080","type":"journal-article","created":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T19:03:37Z","timestamp":1604862217000},"page":"80","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Immunofluorescence Evaluation of Myf5 and MyoD in Masseter Muscle of Unilateral Posterior Crossbite Patients"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3226-8522","authenticated-orcid":false,"given":"Giovanna","family":"Vermiglio","sequence":"first","affiliation":[{"name":"Department of Biomedical and Dental Science and Morphofunctional Imaging, University of Messina, 98166 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8801-8285","authenticated-orcid":false,"given":"Antonio","family":"Centofanti","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Dental Science and Morphofunctional Imaging, University of Messina, 98166 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guglielmo","family":"Ramieri","sequence":"additional","affiliation":[{"name":"Department of Surgical Science, University of Turin, 10124 Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8646-9824","authenticated-orcid":false,"given":"Michele","family":"Tepedino","sequence":"additional","affiliation":[{"name":"Department of Biotechnological and Applied Clinical Science, University of L\u2019Aquila, 67100 L\u2019Aquila, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0473-7248","authenticated-orcid":false,"given":"Michele","family":"Runci Anastasi","sequence":"additional","affiliation":[{"name":"I.R.C.C.S. (Istituto di Ricovero e Cura a Carattere Scientifico), Centro Neurolesi \u2018Bonino Pulejo\u2019, 98100 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Girolamo","family":"Micali","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Dental Science and Morphofunctional Imaging, University of Messina, 98166 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alba","family":"Arco","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Dental Science and Morphofunctional Imaging, University of Messina, 98166 Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0220-5283","authenticated-orcid":false,"given":"Maria Grazia","family":"Piancino","sequence":"additional","affiliation":[{"name":"Department of Surgical Science, University of Turin, 10124 Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1600-0722.2008.00601.x","article-title":"Muscular activation during reverse and non-reverse chewing cycles in unilateral posterior crossbite","volume":"117","author":"Piancino","year":"2009","journal-title":"Eur. J. Oral. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1093\/ejo\/cjr109","article-title":"Reverse-sequencing chewing patterns evaluation in anterior versus posterior unilateral crossbite patients","volume":"34","author":"Piancino","year":"2012","journal-title":"Eur. J. Orthod."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0003-9969(94)90125-2","article-title":"The determination of optimal human jaw movements based on their association with chewing performance","volume":"39","author":"Wilding","year":"1994","journal-title":"Arch. Oral Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1067\/mod.2001.118626","article-title":"Changes in the masticatory cycle following treatment of posterior unilateral crossbite in children","volume":"120","author":"Throckmorton","year":"2001","journal-title":"Am. J. Orthod. Dentofac. Orthop."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1177\/00220345990780111101","article-title":"Morphologic and biochemical changes of the masseter muscles induced by occlusal wear: Studies in a rat model","volume":"78","author":"Bani","year":"1999","journal-title":"J. Dent. Res."},{"key":"ref_6","first-page":"32","article-title":"Evaluation of masseter muscle morphology in different types of malocclusions using cone beam computed tomography","volume":"2","author":"Becht","year":"2014","journal-title":"Int. Orthod."},{"key":"ref_7","first-page":"563463","article-title":"Histological, histochemical, and protein changes after induced malocclusion by occlusion alteration of Wistar rats","volume":"2014","author":"Guerra","year":"2014","journal-title":"Biomed. Res. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3892\/ijmm.2012.986","article-title":"Expression of muscle-specific integrins in masseter muscle fibers during malocclusion disease","volume":"30","author":"Cutroneo","year":"2012","journal-title":"Int. J. Mol. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.4081\/ejh.2016.2605","article-title":"Morphofunctional Compensation of Masseter Muscles in Unilateral Posterior Crossbite Patients","volume":"60","author":"Cutroneo","year":"2016","journal-title":"Eur. J. Histochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1242\/dev.103.3.431","article-title":"Myoblast migration specifically inhibited in the chick embryo by grafted CSAT hybridoma cells secreting an anti- integrin antibody","volume":"103","author":"Jaffredo","year":"1988","journal-title":"Development"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0092-8674(87)90009-2","article-title":"Occupation of the extracellular matrix receptor, integrin, is a control point for myogenic differentiation","volume":"51","author":"Menko","year":"1987","journal-title":"Cell"},{"key":"ref_12","first-page":"37","article-title":"Sarcoglycans subcomplex in normal human smooth muscle: An immunohistochemical and molecular study","volume":"48","author":"Anastasi","year":"2005","journal-title":"Int. J. Mol. Med."},{"key":"ref_13","first-page":"29","article-title":"Sarcoglycan subcomplex in normal and pathological human muscle fibers","volume":"51","author":"Anastasi","year":"2007","journal-title":"Eur. J. Histochem."},{"key":"ref_14","first-page":"605","article-title":"Distrubution of costameric proteins in normal human ventricular and atrial cardiac muscle","volume":"47","author":"Gaeta","year":"2009","journal-title":"Folia Histochem. Cytobiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.4081\/ejh.2015.2509","article-title":"Sarcoglycan complex in masseter and sternocleidomastoid muscles of baboons: An immunohistochemical study","volume":"59","author":"Cutroneo","year":"2015","journal-title":"Eur. J. Histochem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1007\/s00414-018-1840-6","article-title":"Analysis on sarcoglycans expression as markers of septic cardiomyopathy in sepsis-related death","volume":"132","author":"Mondello","year":"2018","journal-title":"J. Leg. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.1242\/jcs.112.17.2895","article-title":"In vivo satellite cell activation via Myf5 and MyoD in regenerating mouse","volume":"112","author":"Cooper","year":"1999","journal-title":"J. Cell Sci."},{"key":"ref_18","first-page":"853","article-title":"Human calf muscles changes after strength training as revealed by diffusion tensor imaging","volume":"59","author":"Bruschetta","year":"2019","journal-title":"J. Sports Med. Phys. Fit."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/0278-2391(89)90380-7","article-title":"Masseter muscle adaptation following surgical correction of vertical maxillary excess","volume":"47","author":"Boyd","year":"1989","journal-title":"J. Oral Maxillofac. Surg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.cjtee.2016.10.005","article-title":"Histomorhological and clinical evaluation of maxillary alveolar ridge reconstruction after craniofacial trauma by applying combination of allogeneic and autogenous bone graft","volume":"20","author":"Falzea","year":"2017","journal-title":"Chin. J. Traumatol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.3892\/or.2013.2766","article-title":"Sarcoglycans and integrins in bisphosphonate treatment: Immunohistochemical and scanning electron microscopy study","volume":"30","author":"Favaloro","year":"2013","journal-title":"Oncol. Rep."},{"key":"ref_22","first-page":"2642","article-title":"Histochemical and morphological aspects of fresh frozen bone: A preliminary study","volume":"60","author":"Cutroneo","year":"2016","journal-title":"Eur. J. Histochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.3892\/etm.2016.3168","article-title":"Effect of bisphosphonates on the mandibular bone and gingival epithelium of rats without tooth extraction","volume":"11","author":"Catalfamo","year":"2016","journal-title":"Exp. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"506","DOI":"10.3389\/fphar.2018.00506","article-title":"Activation of A2A Receptor by PDRN Reduces Neuronal Damage and Stimulates WNT\/\u03b2-CATENIN Driven Neurogenesis in Spinal Cord Injury","volume":"9","author":"Irrera","year":"2018","journal-title":"Front. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e02572","DOI":"10.1016\/j.heliyon.2019.e02572","article-title":"An immunofluorescence study on VEGF and extracellular matrix proteins in human periodontal ligament during tooth movement","volume":"5","author":"Militi","year":"2019","journal-title":"Heliyon"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"37","DOI":"10.5624\/isd.2013.43.1.37","article-title":"The arterial blood supply of the temporomandibular joint: An anatomical study and clinical implications","volume":"43","author":"Cuccia","year":"2013","journal-title":"Imaging Sci. Dent."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1002\/mus.880090808","article-title":"A quantitative comparison of satellite cell ultrastructure in Duchenne muscular dystrophy, polymyositis, and normal controls","volume":"9","author":"Watkins","year":"1986","journal-title":"Muscle Nerve"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1002\/aja.1002010105","article-title":"Enhancement of skeletal muscle regeneration","volume":"201","author":"Bischoff","year":"1994","journal-title":"Dev. Dyn."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0070-2153(08)60758-9","article-title":"The birth of muscle progenitor cells in the mouse: Spatiotemporal considerations","volume":"48","author":"Tajbakhsh","year":"2000","journal-title":"Curr. Top. Dev. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/BF00318695","article-title":"Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes","volume":"267","author":"Grounds","year":"1992","journal-title":"Cell Tissue Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1006\/dbio.1994.1226","article-title":"Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibers","volume":"164","author":"Rivera","year":"1994","journal-title":"Dev. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1006\/excr.2002.5653","article-title":"Kinetics of myoblast proliferation show that resident satellite cells are competent to fully regenerate skeletal muscle fibers","volume":"281","author":"Zammit","year":"2002","journal-title":"Exp. Cell Res."}],"container-title":["Journal of Functional Morphology and Kinesiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2411-5142\/5\/4\/80\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:30:29Z","timestamp":1760178629000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2411-5142\/5\/4\/80"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,7]]},"references-count":32,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["jfmk5040080"],"URL":"https:\/\/doi.org\/10.3390\/jfmk5040080","relation":{},"ISSN":["2411-5142"],"issn-type":[{"type":"electronic","value":"2411-5142"}],"subject":[],"published":{"date-parts":[[2020,11,7]]}}}