{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:16:15Z","timestamp":1760145375336,"version":"build-2065373602"},"reference-count":30,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T00:00:00Z","timestamp":1721260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Imaging"],"abstract":"<jats:p>Identifying the detailed anatomies of the coronary microvasculature remains an area of research; one needs to develop methods for non-destructive, high-resolution, three-dimensional imaging of these vessels for computational modeling. Currently employed Micro-Computed Tomography (Micro-CT) protocols for vasa vasorum analyses require organ dissection and, in most cases, non-clearable contrast agents. Here, we describe a method developed for a non-destructive, economical means to achieve high-resolution images of the human coronary microvasculature without organ dissection. Formalin-fixed human hearts were cannulated using venogram balloon catheters, which were then fixed into the specimen\u2019s aortic root. The canulated hearts, protected by a polyethylene bag, were placed in radiolucent containers filled with insulating polyurethane foam to reduce movement. For vasculature staining, iodine potassium iodide (IKI, Lugol\u2019s solution; 6.3% Potassium Iodide, 4.1% Iodide) was injected. Contrast distributions were monitored using a North Star Imaging X3000 micro-CT scanner with low-radiation settings, followed by high-radiation scanning (3600 rad, 60 kV, 900 mA) for the final high-resolution imaging. We successfully imaged four intact human hearts presenting with chronic total coronary occlusions of the right coronary artery. This imaging enabled detailed analyses of the vasa vasorum surrounding stenosed and occluded segments. After imaging, the hearts were cleared of iodine and excess polyurethane foam and returned to their initial formalin-fixed state for indefinite storage. Conclusions: the described methodologies allow for the non-destructive, high-resolution micro-CT imaging of coronary microvasculature in intact human hearts, paving the way for detailed computational 3D microvascular reconstructions with a macrovascular context.<\/jats:p>","DOI":"10.3390\/jimaging10070173","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T16:51:12Z","timestamp":1721321472000},"page":"173","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-Resolution Iodine-Enhanced Micro-Computed Tomography of Intact Human Hearts for Detailed Coronary Microvasculature Analyses"],"prefix":"10.3390","volume":"10","author":[{"given":"Joerg","family":"Reifart","sequence":"first","affiliation":[{"name":"Visible Heart\u00ae Laboratories, Institute for Engineering in Medicine, Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7661-352X","authenticated-orcid":false,"given":"Paul","family":"Iaizzo","sequence":"additional","affiliation":[{"name":"Visible Heart\u00ae Laboratories, Institute for Engineering in Medicine, Department of Surgery, University of Minnesota, Minneapolis, MN 55455, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/nrcardio.2014.160","article-title":"Coronary microvascular dysfunction: Mechanisms and functional assessment","volume":"12","author":"Camici","year":"2015","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.atherosclerosis.2009.03.010","article-title":"Low vasa vasorum densities correlate with inflammation and subintimal thickening: Potential role in location\u2014Determination of atherogenesis","volume":"206","author":"Versari","year":"2009","journal-title":"Atherosclerosis"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"719","DOI":"10.4244\/EIJ-D-22-00043","article-title":"Prognostic value of structural and functional coronary microvascular dysfunction in patients with non-obstructive coronary artery disease; from the multicentre international ILIAS registry","volume":"18","author":"Boerhout","year":"2022","journal-title":"EuroIntervention"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e1596","DOI":"10.4244\/EIJV11I14A310","article-title":"The collateral circulation of coronary chronic total occlusions","volume":"11","author":"McEntegart","year":"2016","journal-title":"EuroIntervention"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.jcmg.2010.03.013","article-title":"Intravascular and Extravascular Microvessel Formation in Chronic Total Occlusions","volume":"3","author":"Munce","year":"2010","journal-title":"JACC Cardiovasc. Imaging"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.atherosclerosis.2020.12.021","article-title":"Vascular imaging of atherosclerosis: Strengths and weaknesses","volume":"319","author":"Mantella","year":"2021","journal-title":"Atherosclerosis"},{"key":"ref_7","first-page":"526","article-title":"Functional anatomy and hemodynamic characteristics of vasa vasorum in the walls of porcine coronary arteries","volume":"272","author":"Rosol","year":"2003","journal-title":"Anat. Record. Part A Discov. Mol. Cell. Evol. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"101297","DOI":"10.1016\/j.mex.2021.101297","article-title":"Iodine-based contrast staining improves micro-computed tomography of atherosclerotic coronary arteries","volume":"8","author":"Self","year":"2021","journal-title":"MethodsX"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.atherosclerosis.2020.09.012","article-title":"Iodine-enhanced micro-computed tomography of atherosclerotic plaque morphology complements conventional histology","volume":"313","author":"Self","year":"2020","journal-title":"Atherosclerosis"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1056\/NEJM198401193100307","article-title":"Hypothesis: Vasa Vasorum and Neovascularization of Human Coronary Arteries","volume":"310","author":"Barger","year":"1984","journal-title":"N. Engl. J. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10136","DOI":"10.1038\/s41598-024-60254-9","article-title":"Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas","volume":"14","author":"Salg","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Keklikoglou, K., Arvanitidis, C., Chatzigeorgiou, G., Chatzinikolaou, E., Karagiannidis, E., Koletsa, T., Magoulas, A., Makris, K., Mavrothalassitis, G., and Papanagnou, E.-D. (2021). Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques. J. Imaging, 7.","DOI":"10.3390\/jimaging7090172"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"107428","DOI":"10.1016\/j.carpath.2022.107428","article-title":"Diffusible contrast-enhanced micro-CT improves visualization of stented vessels","volume":"60","author":"Self","year":"2022","journal-title":"Cardiovasc. Pathol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"gix027","DOI":"10.1093\/gigascience\/gix027","article-title":"Laboratory X-ray micro-computed tomography: A user guideline for biological samples","volume":"6","author":"Broeckhoven","year":"2017","journal-title":"GigaScience"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Metscher, B.D. (2009). MicroCT for comparative morphology: Simple staining methods allow high-contrast 3D imaging of diverse non-mineralized animal tissues. BMC Physiol., 9.","DOI":"10.1186\/1472-6793-9-11"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1016\/j.jtcvs.2008.10.008","article-title":"Mitral leaflet anatomy revisited","volume":"137","author":"Quill","year":"2009","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1002\/jnr.24539","article-title":"Development of barium-based low viscosity contrast agents for micro CT vascular casting: Application to 3D visualization of the adult mouse cerebrovasculature","volume":"98","author":"Hong","year":"2020","journal-title":"J. Neurosci. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/(SICI)1521-4184(19995)332:5<151::AID-ARDP151>3.0.CO;2-E","article-title":"Iodine and disinfection: Theoretical study on mode of action, efficiency, stability, and analytical aspects in the aqueous system","volume":"332","author":"Gottardi","year":"1999","journal-title":"Arch. Pharm."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"110853","DOI":"10.1016\/j.ejrad.2023.110853","article-title":"Shortage of iodinated contrast media: Status and possible chances\u2014A systematic review","volume":"164","author":"Koeppel","year":"2023","journal-title":"Eur. J. Radiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1111\/joa.12449","article-title":"Diffusible iodine-based contrast-enhanced computed tomography (diceCT): An emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues","volume":"228","author":"Gignac","year":"2016","journal-title":"J. Anat."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1177\/028418519203300622","article-title":"Viscosity of Some Contemporary Contrast Media before and after Mixing with Whole Blood","volume":"33","author":"Smedby","year":"1992","journal-title":"Acta Radiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1063\/1.555581","article-title":"Viscosity of liquid water in the range \u22128 \u00b0C to 150 \u00b0C","volume":"7","author":"Kestin","year":"1978","journal-title":"J. Phys. Chem. Ref. Data"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.atherosclerosis.2023.05.013","article-title":"The role of endothelial shear stress, shear stress gradient, and plaque topography in plaque erosion","volume":"376","author":"Hakim","year":"2023","journal-title":"Atherosclerosis"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1940","DOI":"10.1161\/01.CIR.0000143624.72027.11","article-title":"Growth Factors in the Collateral Circulation of Chronic Total Coronary Occlusions","volume":"110","author":"Werner","year":"2004","journal-title":"Circulation"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Zhang, D., Xing, H., Wang, R., Tian, J., Ju, Z., Zhang, L., Chen, H., He, Y., and Song, X. (2022). A Novel Classification for Predicting Chronic Total Occlusion Percutaneous Coronary Intervention. Front. Cardiovasc. Med., 9.","DOI":"10.3389\/fcvm.2022.762351"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1253\/circj.CJ-19-0914","article-title":"Atherosclerotic Coronary Plaque Is Associated With Adventitial Vasa Vasorum and Local Inflammation in Adjacent Epicardial Adipose Tissue in Fresh Cadavers","volume":"84","author":"Ito","year":"2020","journal-title":"Circ. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.bspc.2018.01.010","article-title":"A review on CT image noise and its denoising","volume":"42","author":"Diwakar","year":"2018","journal-title":"Biomed. Signal Process. Control"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.3390\/tomography9040102","article-title":"A Deep Learning Approach for Rapid and Generalizable Denoising of Photon-Counting Micro-CT Images","volume":"9","author":"Nadkarni","year":"2023","journal-title":"Tomography"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1038\/s41592-021-01317-x","article-title":"Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography","volume":"18","author":"Walsh","year":"2021","journal-title":"Nat. Methods"},{"key":"ref_30","unstructured":"Sheng-Zhuang, M., Jing-Wen, F., Cheng-Wen, Y., and Yu, S. (2019, January 26\u201328). A method for metal artifacts reduction using virtual dual-energy CT images generated from single energy CT scans. Proceedings of the 2019 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments, Beijing, China."}],"container-title":["Journal of Imaging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2313-433X\/10\/7\/173\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:19:13Z","timestamp":1760109553000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2313-433X\/10\/7\/173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,18]]},"references-count":30,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,7]]}},"alternative-id":["jimaging10070173"],"URL":"https:\/\/doi.org\/10.3390\/jimaging10070173","relation":{},"ISSN":["2313-433X"],"issn-type":[{"type":"electronic","value":"2313-433X"}],"subject":[],"published":{"date-parts":[[2024,7,18]]}}}