{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:38:42Z","timestamp":1791049122504,"version":"4.1.0"},"reference-count":122,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute on Alcohol Abuse and Alcoholism","award":["AA-011431"],"award-info":[{"award-number":["AA-011431"]}]},{"name":"National Institute on Alcohol Abuse and Alcoholism","award":["AA-028408"],"award-info":[{"award-number":["AA-028408"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMP"],"abstract":"<jats:p>Background: Alcohol-related brain degeneration (ARBD) is associated with cognitive\u2013motor impairments that can progress to disability and dementia. White matter (WM) is prominently targeted in ARBD due to chronic neurotoxic and degenerative effects on oligodendrocytes and myelin. Early detection and monitoring of WM pathology in ARBD could lead to therapeutic interventions. Objective: This study examines the potential utility of a non-invasive strategy for detecting WM ARBD using exosomes isolated from serum. Comparative analyses were made with paired tissue (Tx) and membrane vesicles (MVs) from the temporal lobe (TL). Methods: Long Evans rats were fed for 8 weeks with isocaloric liquid diets containing 37% or 0% caloric ethanol (n = 8\/group). TL-Tx, TL-MVs, and serum exosomes (S-EVs) were used to examine ethanol\u2019s effects on oligodendrocyte glycoprotein, astrocyte, and oxidative stress markers. Results: Ethanol significantly decreased the TL-Tx expression of platelet-derived growth factor receptor alpha (PDGFRA), 2\u2032,3\u2032-cyclic nucleotide 3\u2032 phosphodiesterase (CNPase), proteolipid protein (PLP), myelin oligodendrocyte glycoprotein (MOG), glial fibrillary acidic protein (GFAP), and 8-OHdG, whereas in the TL-MVs, ethanol increased CNPase, PDGFRA, and 8-OHdG, but decreased MOG and GFAP concordantly with TL-Tx. Ethanol modulated the S-EV expression by reducing PLP, nestin, GFAP, and 4-hydroxynonenal (HNE). Conclusion: Chronic ethanol exposures differentially alter the expression of oligodendrocyte\/myelin, astrocyte, and oxidative stress markers in the brain, brain MVs, and S-EVs. However, directionally concordant effects across all three compartments were limited. Future studies should advance these efforts by characterizing the relationship between ABRD and molecular pathological changes in brain WM-specific exosomes in serum.<\/jats:p>","DOI":"10.3390\/jmp5030025","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T05:53:03Z","timestamp":1725947583000},"page":"360-384","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Brain and Serum Membrane Vesicle (Exosome) Profiles in Experimental Alcohol-Related Brain Degeneration: Forging the Path to Non-Invasive Liquid Biopsy Diagnostics"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5886-2306","authenticated-orcid":false,"given":"Suzanne M.","family":"De La Monte","sequence":"first","affiliation":[{"name":"Department of Medicine, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI 02908, USA"},{"name":"Departments of Pathology and Laboratory Medicine, Neurology, and Neurosurgery, Rhode Island Hospital, Women & Infants Hospital, and the Alpert Medical School of Brown University, Providence, RI 02908, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0069-2181","authenticated-orcid":false,"given":"Yiwen","family":"Yang","sequence":"additional","affiliation":[{"name":"Graduate Program in Biotechnology, Brown University, Providence, RI 02912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Tong","sequence":"additional","affiliation":[{"name":"Department of Medicine, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI 02908, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"key":"ref_1","unstructured":"Centers for Disease Control and Prevention (2008). 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