{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:22:00Z","timestamp":1789428120386,"version":"build-2803163510"},"reference-count":75,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T00:00:00Z","timestamp":1706659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia de Estrat\u00e9gias de Intera\u00e7\u00e3o Pat\u00f3geno-Hospedeiro (INCT-IPH)","award":["20181026700002"],"award-info":[{"award-number":["20181026700002"]}]},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia de Estrat\u00e9gias de Intera\u00e7\u00e3o Pat\u00f3geno-Hospedeiro (INCT-IPH)","award":["202310267000240"],"award-info":[{"award-number":["202310267000240"]}]},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia de Estrat\u00e9gias de Intera\u00e7\u00e3o Pat\u00f3geno-Hospedeiro (INCT-IPH)","award":["202310267000213"],"award-info":[{"award-number":["202310267000213"]}]},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia de Estrat\u00e9gias de Intera\u00e7\u00e3o Pat\u00f3geno-Hospedeiro (INCT-IPH)","award":["202310267000747"],"award-info":[{"award-number":["202310267000747"]}]},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia de Estrat\u00e9gias de Intera\u00e7\u00e3o Pat\u00f3geno-Hospedeiro (INCT-IPH)","award":["407706\/2021-6"],"award-info":[{"award-number":["407706\/2021-6"]}]},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia de Estrat\u00e9gias de Intera\u00e7\u00e3o Pat\u00f3geno-Hospedeiro (INCT-IPH)","award":["312888\/2022-8"],"award-info":[{"award-number":["312888\/2022-8"]}]},{"name":"CNPq (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)","award":["20181026700002"],"award-info":[{"award-number":["20181026700002"]}]},{"name":"CNPq (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)","award":["202310267000240"],"award-info":[{"award-number":["202310267000240"]}]},{"name":"CNPq (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)","award":["202310267000213"],"award-info":[{"award-number":["202310267000213"]}]},{"name":"CNPq (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)","award":["202310267000747"],"award-info":[{"award-number":["202310267000747"]}]},{"name":"CNPq (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)","award":["407706\/2021-6"],"award-info":[{"award-number":["407706\/2021-6"]}]},{"name":"CNPq (Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico)","award":["312888\/2022-8"],"award-info":[{"award-number":["312888\/2022-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The survival of pathogenic fungi in the host after invasion depends on their ability to obtain nutrients, which include the transition metal zinc. This essential micronutrient is required to maintain the structure and function of various proteins and, therefore, plays a critical role in various biological processes. The host\u2019s nutritional immunity limits the availability of zinc to pathogenic fungi mainly by the action of calprotectin, a component of neutrophil extracellular traps. Here we investigated the adaptive responses of Fonsecaea pedrosoi to zinc-limiting conditions. This black fungus is the main etiological agent of chromoblastomycosis, a chronic neglected tropical disease that affects subcutaneous tissues. Following exposure to a zinc-limited environment, F. pedrosoi induces a high-affinity zinc uptake machinery, composed of zinc transporters and the zincophore Pra1. A proteomic approach was used to define proteins regulated by zinc deprivation. Cell wall remodeling, changes in neutral lipids homeostasis, and activation of the antioxidant system were the main strategies for survival in the hostile environment. Furthermore, the downregulation of enzymes required for sulfate assimilation was evident. Together, the adaptive responses allow fungal growth and development and reveals molecules that may be related to fungal persistence in the host.<\/jats:p>","DOI":"10.3390\/jof10020118","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T08:44:06Z","timestamp":1706690646000},"page":"118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Zinc Starvation Induces Cell Wall Remodeling and Activates the Antioxidant Defense System in Fonsecaea pedrosoi"],"prefix":"10.3390","volume":"10","author":[{"given":"Tayn\u00e1 Aparecida de Oliveira","family":"Santos","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Weba","family":"Soares","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"},{"name":"Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06520, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Nojosa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1031-1465","authenticated-orcid":false,"given":"Dayane","family":"Moraes","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Millena Silva","family":"Mendes","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lia Maria de Almeida","family":"Soares","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7754-4047","authenticated-orcid":false,"given":"Alexandre Melo","family":"Bail\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2053-204X","authenticated-orcid":false,"given":"Mirelle Garcia Silva","family":"Bail\u00e3o","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Institute of Biological Sciences, Federal University of Goi\u00e1s, Goi\u00e2nia 74690-900, GO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s10534-008-9184-1","article-title":"The Ins and Outs of Biological Zinc Sites","volume":"22","author":"Auld","year":"2009","journal-title":"Biometals"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1126\/science.1152449","article-title":"Metal chelation and inhibition of bacterial growth in tissue abscesses","volume":"319","author":"Corbin","year":"2008","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1038\/nri.2017.105","article-title":"Neutrophil extracellular traps in immunity and disease","volume":"18","author":"Papayannopoulos","year":"2018","journal-title":"Nat. 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