{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:19:29Z","timestamp":1787764769092,"version":"build-2784847793"},"reference-count":199,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T00:00:00Z","timestamp":1707782400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF)","doi-asserted-by":"publisher","award":["P34304-B"],"award-info":[{"award-number":["P34304-B"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF)","doi-asserted-by":"publisher","award":["TAI534"],"award-info":[{"award-number":["TAI534"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF)","doi-asserted-by":"publisher","award":["P35248"],"award-info":[{"award-number":["P35248"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF)","doi-asserted-by":"publisher","award":["P35619"],"award-info":[{"award-number":["P35619"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF)","doi-asserted-by":"publisher","award":["P28781-B21"],"award-info":[{"award-number":["P28781-B21"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund (FWF)","doi-asserted-by":"publisher","award":["I 4978-B"],"award-info":[{"award-number":["I 4978-B"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Even though fungi are ubiquitous in the biosphere, the ecological knowledge of marine fungi remains rather rudimentary. Also, little is known about their tolerance to salinity and how it influences their activities. Extracellular enzymatic activities (EEAs) are widely used to determine heterotrophic microbes\u2019 enzymatic capabilities and substrate preferences. Five marine fungal species belonging to the most abundant pelagic phyla (Ascomycota and Basidiomycota) were grown under non-saline and saline conditions (0 g\/L and 35 g\/L, respectively). Due to their sensitivity and specificity, fluorogenic substrate analogues were used to determine hydrolytic activity on carbohydrates (\u03b2-glucosidase, \u03b2-xylosidase, and N-acetyl-\u03b2-D-glucosaminidase); peptides (leucine aminopeptidase and trypsin); lipids (lipase); organic phosphorus (alkaline phosphatase), and sulfur compounds (sulfatase). Afterwards, kinetic parameters such as maximum velocity (Vmax) and half-saturation constant (Km) were calculated. All fungal species investigated cleaved these substrates, but some species were more efficient than others. Moreover, most enzymatic activities were reduced in the saline medium, with some exceptions like sulfatase. In non-saline conditions, the average Vmax ranged between 208.5 to 0.02 \u03bcmol\/g biomass\/h, and in saline conditions, 88.4 to 0.02 \u03bcmol\/g biomass\/h. The average Km ranged between 1553.2 and 0.02 \u03bcM with no clear influence of salinity. Taken together, our results highlight a potential tolerance of marine fungi to freshwater conditions and indicate that changes in salinity (due to freshwater input or evaporation) might impact their enzymatic activities spectrum and, therefore, their contribution to the oceanic elemental cycles.<\/jats:p>","DOI":"10.3390\/jof10020152","type":"journal-article","created":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T06:59:26Z","timestamp":1707893966000},"page":"152","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Influence of Salinity on the Extracellular Enzymatic Activities of Marine Pelagic Fungi"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-4489","authenticated-orcid":false,"given":"Katherine","family":"Salazar-Alekseyeva","sequence":"first","affiliation":[{"name":"Bio-Oceanography and Marine Biology Unit, Department of Functional and Evolutionary Ecology, University of Vienna, 1030 Vienna, Austria"},{"name":"Bioprocess Engineering Group, Department of Agrotechnology and Food Sciences, Wageningen University and Research, 6708 WG Wageningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2223-2852","authenticated-orcid":false,"given":"Gerhard J.","family":"Herndl","sequence":"additional","affiliation":[{"name":"Bio-Oceanography and Marine Biology Unit, Department of Functional and Evolutionary Ecology, University of Vienna, 1030 Vienna, Austria"},{"name":"Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research (NIOZ), University of Utrecht, 1790 AB Texel, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8907-1494","authenticated-orcid":false,"given":"Federico","family":"Baltar","sequence":"additional","affiliation":[{"name":"Bio-Oceanography and Marine Biology Unit, Department of Functional and Evolutionary Ecology, University of Vienna, 1030 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1111\/j.1469-185X.1983.tb00395.x","article-title":"Some aspects of the physiology and biochemistry of marine fungi","volume":"58","author":"Jennings","year":"1983","journal-title":"Biol. 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